##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2369242 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.397648277381542 31.0 31.0 34.0 28.0 34.0 2 31.48110534930581 31.0 31.0 34.0 28.0 34.0 3 31.628742019599517 31.0 31.0 34.0 28.0 34.0 4 35.21697656887731 37.0 35.0 37.0 32.0 37.0 5 35.405534343895646 37.0 35.0 37.0 33.0 37.0 6 34.986111169732766 37.0 35.0 37.0 32.0 37.0 7 34.820104911191 37.0 35.0 37.0 32.0 37.0 8 34.85817615929483 37.0 35.0 37.0 32.0 37.0 9 36.281907040310784 39.0 37.0 39.0 32.0 39.0 10 36.07844492035849 39.0 35.0 39.0 31.0 39.0 11 36.25082916814745 39.0 37.0 39.0 31.0 39.0 12 36.21434070474861 39.0 35.0 39.0 31.0 39.0 13 36.074765262476355 39.0 35.0 39.0 30.0 39.0 14 37.14783715635634 40.0 37.0 41.0 31.0 41.0 15 37.20323715348622 40.0 37.0 41.0 31.0 41.0 16 37.25610638339182 40.0 37.0 41.0 31.0 41.0 17 37.33197832893389 40.0 37.0 41.0 31.0 41.0 18 37.35063070804924 40.0 37.0 41.0 31.0 41.0 19 37.3791128132964 40.0 37.0 41.0 31.0 41.0 20 37.43654088522827 40.0 37.0 41.0 31.0 41.0 21 37.26888177737859 40.0 37.0 41.0 31.0 41.0 22 37.28064461123009 40.0 37.0 41.0 31.0 41.0 23 37.29596470094655 40.0 37.0 41.0 31.0 41.0 24 37.211805716765106 40.0 37.0 41.0 30.0 41.0 25 36.176470786859255 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5018663836055559 1101 2 0.4409475218431318 1101 3 0.42059611388294726 1101 4 0.2734181046433761 1101 5 0.3396022846515976 1101 6 0.3797617799612709 1101 7 0.3807123694246073 1101 8 0.39420047998965657 1101 9 0.5752694617872436 1101 10 0.6622823440901797 1101 11 0.5716148885422712 1101 12 0.6836390157823757 1101 13 0.7315384779351461 1101 14 0.8749409456599366 1101 15 0.8340032245985967 1101 16 0.8619334463844126 1101 17 0.7442083721237296 1101 18 0.8369498566044413 1101 19 0.9654102949884518 1101 20 0.8883248874235576 1101 21 1.135257014819949 1101 22 1.1430866030749627 1101 23 1.0085727763984877 1101 24 1.138026648695785 1101 25 1.101127743395601 1102 1 0.36695820788105493 1102 2 0.39601084487941307 1102 3 0.33009225838597445 1102 4 0.2881978196860189 1102 5 0.31264883939340393 1102 6 0.4281335770497279 1102 7 0.40961801019953725 1102 8 0.45063059977272246 1102 9 0.5412907115195225 1102 10 0.5742843412305305 1102 11 0.5275622794464638 1102 12 0.5355937515483049 1102 13 0.6552126780585823 1102 14 0.7022472638890065 1102 15 0.7166226154188138 1102 16 0.6836368972352318 1102 17 0.7707737223400173 1102 18 0.7467616340761651 1102 19 0.86665055318376 1102 20 0.850707949969383 1102 21 1.1483625094579324 1102 22 1.033909444567641 1102 23 0.9767457216591993 1102 24 1.1192431747977452 1102 25 0.9871174869038128 1103 1 0.3496553096553505 1103 2 0.29739121547007485 1103 3 0.23649292663225197 1103 4 0.2641221024425988 1103 5 0.2709074988055704 1103 6 0.24690492696571198 1103 7 0.26162876194409534 1103 8 0.30906704649665784 1103 9 0.291377004995347 1103 10 0.4218403344830648 1103 11 0.5090301460962294 1103 12 0.4909844123515654 1103 13 0.5384262405977864 1103 14 0.7426092089537022 1103 15 0.688423662641334 1103 16 0.6366997468375644 1103 17 0.6068317193563075 1103 18 0.6329036494107285 1103 19 0.6121982323605479 1103 20 0.6282716462553495 1103 21 0.9176225307144037 1103 22 0.740030593457206 1103 23 0.8083151578426566 1103 24 0.9580879147959891 1103 25 0.9234195758234662 1104 1 0.12145207098785349 1104 2 0.12348710435152199 1104 3 0.0731914283270676 1104 4 0.07491715362084506 1104 5 0.13813155453367898 1104 6 0.10457508780462632 1104 7 0.125110404349833 1104 8 0.1544466730111438 1104 9 -0.1261984924622439 1104 10 0.30023842865123385 1104 11 0.36335533454079894 1104 12 0.4403659077905431 1104 13 -0.18007171931505894 1104 14 0.26531097292296835 1104 15 0.4062408491394365 1104 16 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2207 1 0.14087810537154155 2207 2 0.15578520230483406 2207 3 0.17668367969345056 2207 4 0.1557968151615583 2207 5 0.06920589079233253 2207 6 0.25976579329137905 2207 7 0.1775836082732951 2207 8 0.15788366059108938 2207 9 0.3396266981185647 2207 10 0.4308023338760023 2207 11 0.27627197345506005 2207 12 0.35834884957510127 2207 13 0.4186261911594613 2207 14 0.4107912800251654 2207 15 0.4127775311903221 2207 16 0.5165668690129053 2207 17 0.5491629688149189 2207 18 0.49142538713266504 2207 19 0.5953917369251371 2207 20 0.4734157392557421 2207 21 0.8153211132711249 2207 22 0.7480717160029826 2207 23 0.625649840518328 2207 24 0.7967899545142672 2207 25 0.8294430935364048 2208 1 0.11431137528305158 2208 2 0.19382973948735938 2208 3 0.1524232880234031 2208 4 0.0803843496990524 2208 5 0.10949722066155942 2208 6 0.22145671404081213 2208 7 0.23205438434733594 2208 8 0.17277467396912982 2208 9 0.29191604785454217 2208 10 0.34750485545625054 2208 11 0.06289606621987787 2208 12 -0.18689732903002465 2208 13 0.14194578187372997 2208 14 -0.35786689752887924 2208 15 0.14147909675477166 2208 16 0.21794342546076706 2208 17 0.19591167463510573 2208 18 0.25625375433900643 2208 19 0.4826782349045402 2208 20 0.41615093744213283 2208 21 0.721649497956605 2208 22 0.6636485726252843 2208 23 0.6625325013327412 2208 24 0.6819081322662228 2208 25 0.6871313441480567 2209 1 -0.027346089601937962 2209 2 0.00862016851066727 2209 3 0.02617297053160783 2209 4 -0.6088600221669154 2209 5 -0.07306740474835038 2209 6 0.032673016545928135 2209 7 0.06719859681508211 2209 8 0.15646614145398985 2209 9 0.16699558840863205 2209 10 0.18293945559408797 2209 11 0.24523802702962882 2209 12 0.2006936762395597 2209 13 0.3238342833094734 2209 14 0.23409066653997002 2209 15 -0.2683866689694625 2209 16 -0.24341102827605 2209 17 0.10104897815785563 2209 18 -0.2866212274691975 2209 19 0.14757571618098098 2209 20 0.16530187701140164 2209 21 0.5064552504418529 2209 22 0.4731984023534821 2209 23 0.4211890186481213 2209 24 0.6013898290104933 2209 25 0.4755094779309559 2210 1 -0.04737656045006844 2210 2 -0.09541729999224202 2210 3 -0.006975925444542952 2210 4 0.057405968474242286 2210 5 -0.09213751698973027 2210 6 -0.4485439711269663 2210 7 -0.0926382173698741 2210 8 -0.17955198597653066 2210 9 0.04352076400541449 2210 10 0.06554509482734261 2210 11 0.11181244085948094 2210 12 0.21556587023712126 2210 13 0.2825673853403572 2210 14 0.3059406651181362 2210 15 0.17472147709352015 2210 16 0.22312184914739674 2210 17 -0.761541518732507 2210 18 -1.3134924387345137 2210 19 -0.20211979309643624 2210 20 0.006461330271370969 2210 21 -0.18828008690353926 2210 22 -0.1735406951297449 2210 23 -0.052492492928656986 2210 24 0.2931058351039795 2210 25 0.3189700429815048 2211 1 -0.18901341634154534 2211 2 -0.21706045287450237 2211 3 -0.09243089238311342 2211 4 -0.059967694584543096 2211 5 -0.07935760136120962 2211 6 -0.03331582860293736 2211 7 -0.09324141171177303 2211 8 -0.03569296408949896 2211 9 0.02192425945894172 2211 10 0.09297624955451056 2211 11 -0.2422585396260004 2211 12 -0.0783077314821341 2211 13 -0.05308823804257656 2211 14 -0.1672466982432681 2211 15 -0.15645275221334742 2211 16 -0.21308147345069983 2211 17 -0.32297339456668084 2211 18 -0.2979322876388082 2211 19 -0.08996408850655513 2211 20 -0.25815580903636715 2211 21 0.027003942750049248 2211 22 -0.07422196930640723 2211 23 -0.7230411605689753 2211 24 -0.07136306477306675 2211 25 -0.7998333167321334 2212 1 -0.20734878394294398 2212 2 -0.31784918020510133 2212 3 -0.2235729602452281 2212 4 -0.12834529328566902 2212 5 -0.08349960777545107 2212 6 -0.12279580976400695 2212 7 -0.2903323606692041 2212 8 -0.37662541455812004 2212 9 -0.22828303169367103 2212 10 -0.6092455013129197 2212 11 -0.24130371220864077 2212 12 -0.30159431863296504 2212 13 -0.21353488201550874 2212 14 -0.3581770686441672 2212 15 -0.1844963588294064 2212 16 -0.44810837311548113 2212 17 -0.6242333240275784 2212 18 -0.4390534326912814 2212 19 -0.43459619704258046 2212 20 -0.602039856519923 2212 21 -0.05960535412217638 2212 22 -0.3805322173229939 2212 23 -0.3374132549147433 2212 24 -0.41209724089640787 2212 25 -0.21129313497620217 2213 1 -0.20841910331377278 2213 2 -0.20509084699018842 2213 3 -0.1810049200645345 2213 4 -0.034428173070423895 2213 5 -0.12027493662777289 2213 6 -0.26585525344267324 2213 7 -0.35828352900085747 2213 8 -0.3075920848563385 2213 9 -0.2633694940779563 2213 10 -0.1998393617889036 2213 11 0.05845676361867902 2213 12 0.0012154720162769195 2213 13 0.11682788579130232 2213 14 -0.5922037905103394 2213 15 -0.18791435456313366 2213 16 -0.3934636646733978 2213 17 -0.2824467768545773 2213 18 -0.31029261107757833 2213 19 -0.34704829615537847 2213 20 -0.34825081147453574 2213 21 -0.15857071864260064 2213 22 -0.47976402682785846 2213 23 -0.525687820352843 2213 24 -0.585693056091749 2213 25 -0.5060748395609451 2214 1 -0.3139371661148438 2214 2 -0.19200719254692444 2214 3 -0.19503838732699563 2214 4 -0.10510915467753534 2214 5 -0.11158361550934615 2214 6 -0.05505077755872634 2214 7 -0.11455491864449385 2214 8 -0.1377239831694581 2214 9 -0.24318824898765712 2214 10 -0.29953287892706726 2214 11 -0.17744482883845336 2214 12 -0.2973223432085419 2214 13 -0.30019361796308885 2214 14 -0.22791105187195626 2214 15 -0.06094366682303587 2214 16 -0.3720856769797294 2214 17 -0.3549884982911422 2214 18 -0.05899909472301346 2214 19 -0.2986185188329529 2214 20 -0.4851831227412333 2214 21 -0.408163642013065 2214 22 -0.7308202124140593 2214 23 -0.8239328655955518 2214 24 -0.31585806121448456 2214 25 -0.8352444855139822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 11.0 9 33.0 10 88.0 11 149.0 12 377.0 13 781.0 14 1722.0 15 3183.0 16 4771.0 17 6641.0 18 7971.0 19 9062.0 20 9654.0 21 10231.0 22 11043.0 23 12321.0 24 13731.0 25 15343.0 26 17363.0 27 19683.0 28 23236.0 29 29295.0 30 38272.0 31 49926.0 32 67904.0 33 92768.0 34 128082.0 35 181233.0 36 261519.0 37 418606.0 38 934241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50630355060585 21.068857418079194 12.85522958105893 24.56960945025603 2 15.451186497622446 21.81094206501489 38.904130519381305 23.833740917981363 3 17.617214458474926 26.38706751804689 30.820045837265454 25.17567218621273 4 12.08511029885102 18.146175579037834 38.163668391324 31.60504573078714 5 12.584118154660883 38.466250572045666 35.060700167012 13.88893110628144 6 29.69237893538873 39.563835180658195 17.218303185289844 13.52548269866323 7 25.683504657174304 34.4624988020911 22.22998077857535 17.62401576215925 8 24.40103784083176 36.6261054288228 20.877301411200104 18.095555319145344 9 24.903502525922946 16.254332098193807 21.954742315340027 36.88742306054322 10 14.908072829943423 30.142680526285908 33.566699629444145 21.382547014326523 11 31.942369110356715 25.35322444270065 23.831109429257552 18.873297017685083 12 21.12557635391465 27.471927280217322 31.412069201301367 19.990427164566665 13 27.36370002038628 23.303285735811595 27.204661746103497 22.128352497698632 14 20.790005170467136 23.46037206259431 29.019489495510136 26.730133271428418 15 22.62591950403763 30.32717207240213 25.604884662073296 21.442023761486944 16 20.752730833516235 30.55752055511472 27.031410916580846 21.6583376947882 17 19.633325764105145 29.488080998057608 28.891687721220542 21.986905516616705 18 19.880999412467837 28.281654139400448 30.908074852948133 20.92927159518359 19 20.55448277608595 28.289933248495824 31.468934065215144 19.686649910203084 20 21.564320605022807 27.253967845290017 31.23124656798213 19.95046498170505 21 21.826838255466967 27.26940820435331 30.57651642094689 20.32723711923283 22 21.313290384559377 27.310811044669904 30.864236091912005 20.51166247885871 23 21.32660374348208 27.33874773239328 30.736295804811505 20.59835271931313 24 21.181298145693074 27.139830857223895 30.762805472301046 20.916065524781988 25 21.09737464122911 27.418664528110753 30.934366030727674 20.549594799932468 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1368.0 1 1368.0 2 1709.0 3 2050.0 4 2050.0 5 2050.0 6 5259.5 7 8469.0 8 8469.0 9 8469.0 10 10457.0 11 12445.0 12 12445.0 13 12445.0 14 16970.5 15 21496.0 16 21496.0 17 21496.0 18 36115.0 19 50734.0 20 50734.0 21 50734.0 22 74946.0 23 99158.0 24 99158.0 25 99158.0 26 130591.5 27 162025.0 28 162025.0 29 162025.0 30 189632.5 31 217240.0 32 217240.0 33 217240.0 34 233346.0 35 249452.0 36 249452.0 37 249452.0 38 249121.5 39 248791.0 40 248791.0 41 248791.0 42 251587.0 43 254383.0 44 254383.0 45 254383.0 46 258043.0 47 261703.0 48 261703.0 49 261703.0 50 249181.5 51 236660.0 52 236660.0 53 236660.0 54 217068.5 55 197477.0 56 197477.0 57 197477.0 58 171817.5 59 146158.0 60 146158.0 61 146158.0 62 124326.0 63 102494.0 64 102494.0 65 102494.0 66 78175.5 67 53857.0 68 53857.0 69 53857.0 70 40155.0 71 26453.0 72 26453.0 73 26453.0 74 18793.5 75 11134.0 76 11134.0 77 11134.0 78 7519.5 79 3905.0 80 3905.0 81 3905.0 82 2606.0 83 1307.0 84 1307.0 85 1307.0 86 832.5 87 358.0 88 358.0 89 358.0 90 230.5 91 103.0 92 103.0 93 103.0 94 60.0 95 17.0 96 17.0 97 17.0 98 11.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09800602893246026 2 0.0 3 0.12852211804450536 4 0.011353842283734629 5 0.023214175673063366 6 0.013548637074642437 7 0.022412231422539362 8 0.02017522903949871 9 3.798683291955824E-4 10 0.38003715956411377 11 0.06263606672513825 12 0.0016038885010480144 13 4.2207592132842485E-5 14 7.175290662583222E-4 15 0.0012662277639852748 16 8.441518426568497E-5 17 0.0 18 4.2207592132842485E-4 19 2.954531449298974E-4 20 3.798683291955824E-4 21 5.064911055941098E-4 22 0.0026590783043690764 23 1.6883036853136994E-4 24 4.2207592132842485E-5 25 0.0017727188695793846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2369242.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.51481867965792 #Duplication Level Percentage of deduplicated Percentage of total 1 84.10135531070468 58.46290465137122 2 11.238481728850179 15.624820392313374 3 2.5405068841329226 5.298086262147685 4 0.8345800927562775 2.3206273528641894 5 0.3744100717369869 1.3013524124317182 6 0.21487969533257995 0.8962393835390661 7 0.13818743836474462 0.6724252305212138 8 0.09598593454546903 0.5337955868580635 9 0.07103388920201184 0.4444117135189887 >10 0.3361553606733804 4.123146034768312 >50 0.029213005574898906 1.4053008690609037 >100 0.02112490699928543 2.8864381192270914 >500 0.0024392126128714273 1.1978242789296574 >1k 0.0013415669370770921 1.6241440854604525 >5k 1.2196063064337201E-4 0.6198741703212454 >10k+ 1.8294094596505803E-4 2.588609456666701 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 26250 1.1079492934871153 No Hit GGTATCAACGCAGAGTACTTTTTTT 17854 0.7535743499397698 No Hit TATCAACGCAGAGTACTTTTTTTTT 16962 0.7159251777572743 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 8952 0.37784236477320593 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5671 0.23935925498534974 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4688 0.1978691919187656 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3288 0.1387785629327861 No Hit GTACTGGTTCACTATCGGTCAGTCA 3122 0.13177210263873423 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2520 0.10636313217476306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.2207592132842485E-5 0.0 6 0.0 0.0 0.0 4.2207592132842485E-5 0.0 7 0.0 0.0 0.0 4.2207592132842485E-5 0.0 8 0.0 0.0 0.0 4.2207592132842485E-5 0.0 9 0.0 0.0 0.0 8.441518426568497E-5 0.0 10 0.0 0.0 0.0 8.441518426568497E-5 0.0 11 0.0 0.0 0.0 8.441518426568497E-5 0.0 12 0.0 0.0 0.0 8.441518426568497E-5 0.0 13 0.0 0.0 0.0 1.6883036853136994E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCGCC 100 1.8189894E-12 16.119646 18 GGACCGT 60 1.4321031E-6 15.870565 6 GACGAAC 50 8.8611836E-5 15.170793 13 GGTATCA 3140 0.0 15.015559 1 AAATCGC 125 0.0 14.412861 17 ATCGCCG 100 6.7848305E-10 14.223217 19 GGCGATC 55 0.0031128074 12.068185 18 TTTCGTC 70 0.0015154977 10.836737 17 GTATCAA 7070 0.0 10.823458 1 CTAGTAC 160 6.206392E-9 10.090752 3 GAAATCG 165 1.0546501E-8 9.769483 16 AGGACTG 340 0.0 9.522339 5 CGAGACT 80 0.00443024 9.522339 4 GTTCTAG 160 6.5760105E-8 9.505818 1 CCCCCGG 320 0.0 9.482145 18 TAGACTG 185 6.4355845E-9 9.264978 5 GTCTAAG 195 1.6643753E-9 9.262079 1 CTAGACT 145 1.6532867E-6 9.193982 4 GCGCCTT 375 0.0 9.102475 13 GTCCTAC 115 1.1035548E-4 9.092522 1 >>END_MODULE