FastQCFastQC Report
Wed 25 May 2016
SRR1294631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830175
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC67450.3685439916947833No Hit
CCCATGTACTCTGCGTTGATACCAC66160.36149548540440124No Hit
GTATCAACGCAGAGTACTTTTTTTT45470.24844618684005626No Hit
GGTATCAACGCAGAGTACTTTTTTT29330.16025789883481087No Hit
GAGTACATGGGAAGCAGTGGTATCA28710.15687024464873578No Hit
TATCAACGCAGAGTACTTTTTTTTT27190.14856502793448714No Hit
CATGTACTCTGCGTTGATACCACTG23340.12752878823063368No Hit
GCGTTGATACCACTGCTTCCCATGT20790.11359569440080866No Hit
ATTCCATTCCATTCCATTCCATTCC19880.10862349228898875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGC752.0785544E-411.3974246
GTACGAC600.005886223611.080833
CTGCCGA600.005887421311.0805269
TGTATCG1355.7065336E-810.5531715
TGTCCCG2300.010.322906511
TCGCGCG1303.854875E-710.2281789
TCGATCT750.00263971310.13714114
TATCGCG1252.5773606E-69.8777687
CGCGCGC1252.578261E-69.87749810
CGCGCCT1356.414248E-79.85501512
GTGCTAG1356.445516E-79.8515121
GCGCGCC1356.4696724E-79.84881911
GTCTAGA1752.57387E-99.77109051
CCCCCGG1804.149115E-99.50408918
CGCCCTA2500.09.5035716
GTCTAGG1401.0589429E-69.4996721
GTCTAAG1502.6491216E-79.4996721
TAAGACT2106.730261E-119.4978544
CTAGACT1304.2504853E-69.4978534
CTTGGAC1502.6565067E-79.4978533
Produced by FastQC (version 0.11.5)