FastQCFastQC Report
Wed 25 May 2016
SRR1294631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830175
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT119600.6534894204106164No Hit
GGTATCAACGCAGAGTACTTTTTTT81050.44285382545385005No Hit
CCCATGTACTCTGCGTTGATACCAC74760.4084855273402817No Hit
TATCAACGCAGAGTACTTTTTTTTT74760.4084855273402817No Hit
GTACATGGGAAGCAGTGGTATCAAC68630.3749914625650553No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43520.23779146802901363No Hit
GAGTACATGGGAAGCAGTGGTATCA32890.17970959061291952No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30290.16550329886486265No Hit
CATGTACTCTGCGTTGATACCACTG28750.15708880298332126No Hit
GCGTTGATACCACTGCTTCCCATGT23490.12834838198532927No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23370.1276927069815728No Hit
ACGCAGAGTACATGGGAAGCAGTGG19210.1049626401846818No Hit
ATTCCATTCCATTCCATTCCATTCC19180.10479872143374268No Hit
GTATCAACGCAGAGTACATGGGAAG18670.10201210266777766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC2050.012.5117453
CCCTATA1501.7644197E-1011.399592
GTCTAGA1909.094947E-1210.51026251
TCAAGAC2151.8189894E-1210.1624253
TATCGCG1553.9530278E-89.8238387
CTTAGAC1651.0224539E-89.7875263
GTACTGG5650.09.76174451
CGCCTTT1752.6411726E-99.75666114
TAAGACA2350.09.719334
TATACTG5300.09.6964065
CTATACT2900.09.5168444
ACGCTGA800.00445057179.5168446
TCCGTAC800.00445057179.5168448
ATCGCGC1606.455048E-89.5168448
TCGCGCG1606.457776E-89.5165839
CCGGGGG1304.3206746E-69.48564316
TCTAGAC1951.6825652E-99.2560773
TCAGGAC1951.6825652E-99.2560773
CGCGCGC1752.5940608E-89.2446810
TGTATCG1651.0367148E-79.2284555
Produced by FastQC (version 0.11.5)