##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1830175 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379841272009507 31.0 31.0 34.0 28.0 34.0 2 31.46421353149289 31.0 31.0 34.0 28.0 34.0 3 31.637031431420493 31.0 31.0 34.0 28.0 34.0 4 35.22291201661044 37.0 35.0 37.0 32.0 37.0 5 35.40130807163249 37.0 35.0 37.0 33.0 37.0 6 35.00057590121163 37.0 35.0 37.0 32.0 37.0 7 34.8357698034341 37.0 35.0 37.0 32.0 37.0 8 34.87496004480446 37.0 35.0 37.0 32.0 37.0 9 36.31478355894928 39.0 37.0 39.0 32.0 39.0 10 36.09512478314915 39.0 35.0 39.0 31.0 39.0 11 36.27301760760583 39.0 37.0 39.0 32.0 39.0 12 36.22038821424181 39.0 35.0 39.0 31.0 39.0 13 36.09161091152485 39.0 35.0 39.0 30.0 39.0 14 37.18805469422323 40.0 37.0 41.0 31.0 41.0 15 37.235445790703075 40.0 37.0 41.0 31.0 41.0 16 37.26819675714071 40.0 37.0 41.0 31.0 41.0 17 37.317511166964906 40.0 37.0 41.0 31.0 41.0 18 37.3180985424891 40.0 37.0 41.0 31.0 41.0 19 37.31794883002991 40.0 37.0 41.0 31.0 41.0 20 37.38337645307143 40.0 37.0 41.0 31.0 41.0 21 37.22670892127802 40.0 37.0 41.0 30.0 41.0 22 37.23580477276763 40.0 37.0 41.0 31.0 41.0 23 37.24933954403267 40.0 37.0 41.0 31.0 41.0 24 37.18269345827585 40.0 37.0 41.0 30.0 41.0 25 36.13243815482126 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4581567792526151 1101 2 0.4551568410388569 1101 3 0.42852080104932355 1101 4 0.30880395474687816 1101 5 0.37008491578019687 1101 6 0.3928847489647751 1101 7 0.5738952380441162 1101 8 0.5455704908129277 1101 9 0.7046602914777651 1101 10 0.7429062097405605 1101 11 0.7259538684294284 1101 12 0.8070848356985039 1101 13 0.6522575386292999 1101 14 0.817298681889028 1101 15 0.8961366948239231 1101 16 1.0264243933347643 1101 17 0.9023478650951375 1101 18 1.0162222697970407 1101 19 0.9521494071215315 1101 20 0.9379364175520877 1101 21 1.2283521109627102 1101 22 1.2181692205635173 1101 23 1.1240182748195693 1101 24 1.3104251070246065 1101 25 1.1392185275791746 1102 1 0.44204384859282086 1102 2 0.3663039438496334 1102 3 0.3303529553835567 1102 4 0.2748289310471108 1102 5 0.3316673413355531 1102 6 0.3874077933492188 1102 7 0.43620405709427246 1102 8 0.4564439768514603 1102 9 0.6349706642741992 1102 10 0.8024442953471862 1102 11 0.7070120243995461 1102 12 0.7283261557668084 1102 13 0.7136590930600448 1102 14 0.9569460773106044 1102 15 0.877073870770289 1102 16 0.8843281363574533 1102 17 0.82922596445961 1102 18 0.7812433001293115 1102 19 0.9828001324883218 1102 20 0.8993547414910736 1102 21 1.2558345970936458 1102 22 1.1454537238267264 1102 23 0.9552930654573899 1102 24 1.1156895347908034 1102 25 0.9967297272150404 1103 1 0.2695004959916645 1103 2 0.30936752766466213 1103 3 0.21937029642402095 1103 4 0.1826323992552048 1103 5 0.21345924885000755 1103 6 0.25706097253996774 1103 7 0.31915203397288394 1103 8 0.3133803930364323 1103 9 0.338149855025641 1103 10 0.5839471855206 1103 11 0.5026767642839332 1103 12 0.5121411846685433 1103 13 0.6945839485513758 1103 14 0.7919171755765007 1103 15 0.7299640441806972 1103 16 0.6253685987851938 1103 17 0.6338502419162566 1103 18 0.6815323174703565 1103 19 0.8599677625461268 1103 20 0.6528229495835589 1103 21 0.934158296515605 1103 22 0.8000780012833744 1103 23 0.8145416203706901 1103 24 0.8723369336347346 1103 25 0.8767875306832451 1104 1 0.07713372110845995 1104 2 0.16953097422944552 1104 3 0.008097960266500337 1104 4 -0.03662618548362673 1104 5 0.0857947465141109 1104 6 -0.08529953370227616 1104 7 0.027300937290647198 1104 8 0.007531751022021638 1104 9 -0.11373502633207977 1104 10 0.24177935750571322 1104 11 0.21759448012313953 1104 12 0.22480383940499848 1104 13 -0.19619224117069223 1104 14 0.08067948190848995 1104 15 0.28404657908712494 1104 16 0.42446327505938086 1104 17 0.4066139867363745 1104 18 0.43732487164460565 1104 19 0.4842537973337855 1104 20 0.4733382318703576 1104 21 0.6519421009868012 1104 22 0.6245791494344601 1104 23 0.5996021292523608 1104 24 0.5770440231377307 1104 25 0.6223826556601466 1105 1 0.01683281077150056 1105 2 0.010205995555150338 1105 3 -0.10836383436760855 1105 4 -0.11066269931826156 1105 5 0.00848443966162904 1105 6 -0.044997085009420346 1105 7 -0.02403574873421377 1105 8 -0.13764629551688046 1105 9 0.11583801718292364 1105 10 0.17281826272450473 1105 11 0.023944027595618422 1105 12 -0.1985451019684561 1105 13 -0.5617744329515162 1105 14 -0.759305590097128 1105 15 -0.1627814366846536 1105 16 0.042871783157863774 1105 17 0.0895555344940746 1105 18 -0.6389011583936082 1105 19 -0.792951663631591 1105 20 -0.6343646726512162 1105 21 0.11287281290886142 1105 22 0.20055841530196972 1105 23 0.11614985393815402 1105 24 0.32197553658943434 1105 25 0.21044643338687052 1106 1 -0.46998672101805283 1106 2 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0.13507014243023718 2207 1 0.27322665955056635 2207 2 0.302427957989881 2207 3 0.25138783613434157 2207 4 0.24266743452698591 2207 5 0.17518802349847107 2207 6 0.3111326009074986 2207 7 0.3223067851783057 2207 8 0.40693734033215634 2207 9 0.5082798418755914 2207 10 0.5537870513080705 2207 11 0.4277050668549762 2207 12 0.40002374240106064 2207 13 0.5178764126567756 2207 14 0.5903943553474988 2207 15 0.4742981690399688 2207 16 0.6308843678109923 2207 17 0.570190427782947 2207 18 0.6128124135887205 2207 19 0.812210997522115 2207 20 0.5474667471421313 2207 21 0.9899509419466739 2207 22 0.6299440725711634 2207 23 0.7160661750190869 2207 24 0.910367547713804 2207 25 0.8196064036890576 2208 1 0.1103282416564042 2208 2 0.06792321167236892 2208 3 0.09648243515234256 2208 4 0.060791166114547934 2208 5 0.06823766888854266 2208 6 0.19224247543014172 2208 7 0.23696166788425188 2208 8 0.134258235040285 2208 9 0.2835841517501123 2208 10 0.3191880333047976 2208 11 -0.07508816827868259 2208 12 -0.2528692199557341 2208 13 0.022758900381816716 2208 14 -0.5297876413791798 2208 15 -0.0650995396343319 2208 16 0.2327628184383741 2208 17 0.188199374864233 2208 18 0.31421208625648234 2208 19 0.449388957151136 2208 20 0.393021793514194 2208 21 0.5751594525849697 2208 22 0.6105088297997554 2208 23 0.5925724488870685 2208 24 0.7237257582975474 2208 25 0.7234780894501043 2209 1 0.07948823087182433 2209 2 0.032021063743904676 2209 3 0.06189543056889946 2209 4 -0.5414983279089967 2209 5 -0.111706610791515 2209 6 0.08581184722847013 2209 7 0.1285473962926318 2209 8 0.19514393648721295 2209 9 0.19415275065367865 2209 10 0.23887707797092617 2209 11 0.28320966581855345 2209 12 0.2702621901278519 2209 13 0.32862729258097545 2209 14 0.3918029400893204 2209 15 -0.20474149164296307 2209 16 -0.25057867522568245 2209 17 0.13864984668951763 2209 18 -0.16146027826225406 2209 19 0.22327978127264458 2209 20 0.3206046460350933 2209 21 0.4212566216332334 2209 22 0.5658562909303342 2209 23 0.46938704401603104 2209 24 0.5500503381483597 2209 25 0.6068591265670307 2210 1 -0.2779078239173671 2210 2 -0.30285006525341984 2210 3 -0.11298176368424961 2210 4 -0.08190314024892587 2210 5 -0.1201564788287115 2210 6 -0.572870586441887 2210 7 -0.17405796899218018 2210 8 -0.36517684340145706 2210 9 -0.14560266298635582 2210 10 0.11358605904074182 2210 11 0.05680324719813257 2210 12 0.04705970653818525 2210 13 0.07545454642447424 2210 14 0.0813566561706125 2210 15 -0.13377643016844587 2210 16 0.05268539908353631 2210 17 -0.8707645595619979 2210 18 -1.5256351903167626 2210 19 -0.4377436358331721 2210 20 -0.3465788410706807 2210 21 -0.48497507940868445 2210 22 -0.5041696824512627 2210 23 -0.34272034152365194 2210 24 0.05824477821646212 2210 25 0.13866215431878004 2211 1 -0.2839311549669006 2211 2 -0.2773210359519531 2211 3 -0.22809614220719254 2211 4 -0.1320906864548732 2211 5 -0.19577930684722844 2211 6 -0.046555863900053396 2211 7 -0.19721484286397128 2211 8 -0.26391074733816566 2211 9 -0.06519732255563326 2211 10 -0.14333987517384372 2211 11 -0.4848621326665423 2211 12 -0.33997112972600263 2211 13 -0.19435123892992578 2211 14 -0.25048062043629216 2211 15 -0.33681943999414443 2211 16 -0.3591428555167866 2211 17 -0.47047053933449234 2211 18 -0.442323092265525 2211 19 -0.25313872990486175 2211 20 -0.38032069195494245 2211 21 -0.039714391865622645 2211 22 -0.3115708454470436 2211 23 -0.9145027425512566 2211 24 -0.44085075245067884 2211 25 -1.0980602812483014 2212 1 -0.14045669558209184 2212 2 -0.2958525205452034 2212 3 -0.20907070292296837 2212 4 -0.08629279483384522 2212 5 -0.004342526369477184 2212 6 -0.1795366040677635 2212 7 -0.16147292067920205 2212 8 -0.2929953214450691 2212 9 -0.08998237544653875 2212 10 -0.6027366528876712 2212 11 -0.23132247790211835 2212 12 -0.3008089689097062 2212 13 -0.24615379138945315 2212 14 -0.1955248798729059 2212 15 -0.06024040434841993 2212 16 -0.31434568923247497 2212 17 -0.501242187199928 2212 18 -0.4479837296430986 2212 19 -0.5491784068656003 2212 20 -0.6512426416322512 2212 21 -0.08611362970233927 2212 22 -0.45524402933553176 2212 23 -0.1677678579469628 2212 24 -0.4860014383551743 2212 25 -0.2431651462542206 2213 1 -0.1605830196048572 2213 2 -0.2798704820213267 2213 3 -0.08552477205486397 2213 4 -0.08486063568724944 2213 5 -0.11201007885920689 2213 6 -0.24947869574103265 2213 7 -0.3398586023860162 2213 8 -0.3144593325272851 2213 9 -0.3660451275291692 2213 10 -0.3942466513798877 2213 11 -0.13835393404445284 2213 12 0.06350555481904507 2213 13 0.02831565713857742 2213 14 -0.6137971008386387 2213 15 -0.24463107116586968 2213 16 -0.291128172552618 2213 17 -0.28285287738802367 2213 18 -0.32249016599577374 2213 19 -0.35411239420021445 2213 20 -0.3769178461254441 2213 21 -0.10209189876638192 2213 22 -0.3290375665966394 2213 23 -0.6024680916534777 2213 24 -0.7380553227835307 2213 25 -0.5152322709102535 2214 1 -0.13343818860364465 2214 2 -0.07509522804394564 2214 3 -0.011296288251912756 2214 4 0.011489038454975287 2214 5 0.020341687801071373 2214 6 -0.05488849383699801 2214 7 -0.003025093345058849 2214 8 0.04750835324359315 2214 9 0.005506610378951393 2214 10 0.12680032644900763 2214 11 0.21562105157946831 2214 12 -0.03810137125596924 2214 13 -0.09664440931085494 2214 14 0.029837890535709732 2214 15 0.11846074405360696 2214 16 -0.18917382458762688 2214 17 -0.2019954241901445 2214 18 -0.06026450948819928 2214 19 -0.13300631471647506 2214 20 -0.3293290339089694 2214 21 -0.382980500133705 2214 22 -0.6878864420678568 2214 23 -0.8185764872831456 2214 24 -0.029757929645711556 2214 25 -0.5419138727248338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 13.0 9 27.0 10 47.0 11 134.0 12 280.0 13 692.0 14 1345.0 15 2597.0 16 3692.0 17 4851.0 18 5899.0 19 6653.0 20 7224.0 21 7784.0 22 8537.0 23 9310.0 24 10777.0 25 11905.0 26 13934.0 27 15639.0 28 18369.0 29 23101.0 30 29963.0 31 39322.0 32 52803.0 33 71971.0 34 99004.0 35 139622.0 36 201401.0 37 323650.0 38 719626.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.439597208989944 21.669596301113188 12.142304760008795 24.74850172988807 2 14.512710533145738 21.92593604436734 39.89033835562173 23.671015066865188 3 17.67689339211772 27.8440612651627 30.19738796437268 24.2816573783469 4 11.962187110305264 17.995440332510363 38.553781015739155 31.488591541445217 5 12.912895516482484 38.33290250088671 35.02137675832923 13.732825224301573 6 30.146909351618493 39.203714592354416 17.445622118808377 13.203753937218712 7 25.055648188680834 33.935366671421285 23.918802010494048 17.09018312940383 8 23.92040812422978 37.97205758989198 21.288039631988674 16.819494653889556 9 24.566134148993484 16.65256964638335 22.42868710843887 36.352609096184295 10 15.106965876774654 30.180365227931517 33.170089593005876 21.542579302287955 11 32.23961044357643 25.985755777078897 23.006399293147116 18.76823448619756 12 20.85429016512845 27.74880324082548 32.15603521239505 19.240871381651022 13 26.856174955946837 23.759039436119497 27.23570150395454 22.149084103979128 14 20.35453692077532 22.856173387929594 28.91274105789542 27.87654863339967 15 23.332639402279483 29.658831802587 24.762028092733228 22.24650070240029 16 21.249357301195023 30.72403537807628 26.698951410604355 21.327655910124342 17 19.958102344367255 29.903659433474633 29.280166803812097 20.858071418346018 18 20.31368670669938 29.013042504248236 30.324778572482337 20.348492216570047 19 20.709783385532663 28.413246026039197 30.69555515243443 20.181415435993713 20 22.05593566513348 27.579126356291805 29.75525824320529 20.609679735369426 21 22.0207171784947 28.442408691642097 29.19818465149724 20.338689478365968 22 20.857760304680305 28.180141685085363 29.973744819314636 20.988353190919696 23 20.588174226247588 28.522947569955175 30.057393512740877 20.83148469105636 24 20.75446337098911 27.892961055636757 29.92959689647165 21.42297867690248 25 21.36874774607407 27.53292097872294 29.25907307637667 21.839258198826318 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1283.0 1 1283.0 2 1391.5 3 1500.0 4 1500.0 5 1500.0 6 3431.5 7 5363.0 8 5363.0 9 5363.0 10 7088.0 11 8813.0 12 8813.0 13 8813.0 14 13145.0 15 17477.0 16 17477.0 17 17477.0 18 27726.5 19 37976.0 20 37976.0 21 37976.0 22 54718.5 23 71461.0 24 71461.0 25 71461.0 26 93272.5 27 115084.0 28 115084.0 29 115084.0 30 137250.5 31 159417.0 32 159417.0 33 159417.0 34 176083.0 35 192749.0 36 192749.0 37 192749.0 38 200791.0 39 208833.0 40 208833.0 41 208833.0 42 209195.0 43 209557.0 44 209557.0 45 209557.0 46 217529.5 47 225502.0 48 225502.0 49 225502.0 50 208515.5 51 191529.0 52 191529.0 53 191529.0 54 167727.0 55 143925.0 56 143925.0 57 143925.0 58 123171.0 59 102417.0 60 102417.0 61 102417.0 62 86219.5 63 70022.0 64 70022.0 65 70022.0 66 53739.5 67 37457.0 68 37457.0 69 37457.0 70 28042.0 71 18627.0 72 18627.0 73 18627.0 74 12983.0 75 7339.0 76 7339.0 77 7339.0 78 5010.5 79 2682.0 80 2682.0 81 2682.0 82 1763.5 83 845.0 84 845.0 85 845.0 86 544.0 87 243.0 88 243.0 89 243.0 90 153.5 91 64.0 92 64.0 93 64.0 94 35.5 95 7.0 96 7.0 97 7.0 98 5.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09469039845916374 2 0.0 3 0.12304834237163112 4 0.011856789651262857 5 0.020981600120207083 6 0.011091835480213641 7 0.020544483451036103 8 0.01857745843976669 9 5.463958364637262E-5 10 0.3786523146693622 11 0.06682421079951371 12 0.0013113500075129427 13 0.0 14 7.10314587402844E-4 15 0.0012020708402201976 16 1.0927916729274524E-4 17 5.463958364637262E-5 18 2.731979182318631E-4 19 6.010354201100988E-4 20 5.463958364637262E-5 21 1.6391875093911784E-4 22 0.0032783750187823572 23 1.6391875093911784E-4 24 0.0 25 0.0019123854276230415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1830175.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.75010138774344 #Duplication Level Percentage of deduplicated Percentage of total 1 85.77724546076215 62.403033040318014 2 10.332261593303164 15.033461569549855 3 2.167931693896824 4.731517515980889 4 0.6997560529559166 2.036292951969204 5 0.3236497492581676 1.1772776036324717 6 0.17797703949189175 0.7768708600635327 7 0.10456711496874586 0.532508775106006 8 0.06601779857606077 0.38422412318432364 9 0.049571959221807356 0.3245728553438012 >10 0.25586623020376703 3.4036395138140176 >50 0.024501573919500648 1.2382640489888037 >100 0.016132142264181654 2.230999740088793 >500 0.0026375948537349365 1.2761468473817696 >1k 0.0015071970592771054 2.155145962562931 >5k 3.0143941185542105E-4 1.640345133550824 >10k+ 7.535985296385526E-5 0.6556994584648347 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11960 0.6534894204106164 No Hit GGTATCAACGCAGAGTACTTTTTTT 8105 0.44285382545385005 No Hit CCCATGTACTCTGCGTTGATACCAC 7476 0.4084855273402817 No Hit TATCAACGCAGAGTACTTTTTTTTT 7476 0.4084855273402817 No Hit GTACATGGGAAGCAGTGGTATCAAC 6863 0.3749914625650553 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4352 0.23779146802901363 No Hit GAGTACATGGGAAGCAGTGGTATCA 3289 0.17970959061291952 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3029 0.16550329886486265 No Hit CATGTACTCTGCGTTGATACCACTG 2875 0.15708880298332126 No Hit GCGTTGATACCACTGCTTCCCATGT 2349 0.12834838198532927 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2337 0.1276927069815728 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1921 0.1049626401846818 No Hit ATTCCATTCCATTCCATTCCATTCC 1918 0.10479872143374268 No Hit GTATCAACGCAGAGTACATGGGAAG 1867 0.10201210266777766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 205 0.0 12.511745 3 CCCTATA 150 1.7644197E-10 11.39959 2 GTCTAGA 190 9.094947E-12 10.5102625 1 TCAAGAC 215 1.8189894E-12 10.162425 3 TATCGCG 155 3.9530278E-8 9.823838 7 CTTAGAC 165 1.0224539E-8 9.787526 3 GTACTGG 565 0.0 9.7617445 1 CGCCTTT 175 2.6411726E-9 9.756661 14 TAAGACA 235 0.0 9.71933 4 TATACTG 530 0.0 9.696406 5 CTATACT 290 0.0 9.516844 4 ACGCTGA 80 0.0044505717 9.516844 6 TCCGTAC 80 0.0044505717 9.516844 8 ATCGCGC 160 6.455048E-8 9.516844 8 TCGCGCG 160 6.457776E-8 9.516583 9 CCGGGGG 130 4.3206746E-6 9.485643 16 TCTAGAC 195 1.6825652E-9 9.256077 3 TCAGGAC 195 1.6825652E-9 9.256077 3 CGCGCGC 175 2.5940608E-8 9.24468 10 TGTATCG 165 1.0367148E-7 9.228455 5 >>END_MODULE