FastQCFastQC Report
Wed 25 May 2016
SRR1294630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3913933
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC669571.7107344453775781No Hit
CCCATGTACTCTGCGTTGATACCAC665271.699748053939605No Hit
GAGTACATGGGAAGCAGTGGTATCA300470.7676932640390114No Hit
CATGTACTCTGCGTTGATACCACTG249690.6379516460808092No Hit
GCGTTGATACCACTGCTTCCCATGT207380.5298506642806609No Hit
GTATCAACGCAGAGTACATGGGAAG170580.43582759336963617No Hit
ACGCAGAGTACATGGGAAGCAGTGG159650.4079017193191606No Hit
TATCAACGCAGAGTACATGGGAAGC141330.36109458184388954No Hit
ACTCTGCGTTGATACCACTGCTTCC139600.35667447552117015No Hit
GGTATCAACGCAGAGTACATGGGAA114870.29348994987906024No Hit
GTATCAACGCAGAGTACTTTTTTTT113410.28975968673965546No Hit
GCTTCCCATGTACTCTGCGTTGATA109540.2798719344454798No Hit
GTACTGGTTCACTATCGGTCAGTCA99020.25299360004374116No Hit
CAGTGGTATCAACGCAGAGTACATG83070.21224175273312035No Hit
GTGGTATCAACGCAGAGTACATGGG77510.19803609310634598No Hit
ATACCACTGCTTCCCATGTACTCTG76740.19606876254652292No Hit
GGTATCAACGCAGAGTACTTTTTTT69170.1767276036661844No Hit
GTACTCTGCGTTGATACCACTGCTT68860.1759355614927491No Hit
TATCAACGCAGAGTACTTTTTTTTT67430.17228194759593482No Hit
GTTAATGATAGTGTGTCGAAACACA67040.1712855074422582No Hit
GCAGTGGTATCAACGCAGAGTACAT66570.17008466930834024No Hit
GGATACCACGTGTCCCGCCCTACTC65670.16778519203062495No Hit
CATGGGAAGCAGTGGTATCAACGCA61870.15807628796916043No Hit
CTGCTTCCCATGTACTCTGCGTTGA61670.15756529301855704No Hit
GGGAAGCAGTGGTATCAACGCAGAG61520.1571820468056045No Hit
TACCACTGCTTCCCATGTACTCTGC58560.14961932153667423No Hit
GCAGAGTACATGGGAAGCAGTGGTA55120.14083020838629584No Hit
ACATGGGAAGCAGTGGTATCAACGC52530.13421282377598184No Hit
GTTGATACCACTGCTTCCCATGTAC51010.13032926215139604No Hit
AGCGTACACGGTGGATGCCCTGGCA50740.12963941896808145No Hit
AAGCAGTGGTATCAACGCAGAGTAC49970.1276720884082584No Hit
GTACATGGTAAGCAGTGGTATCAAC49720.12703334472000416No Hit
GTACATGGGGGTTAAGCGACTAAGC49600.12672674774964213No Hit
ATACAGGGTGACAGCCCCGTACACA48180.123098683600358No Hit
CCATGTACTCTGCGTTGATACCACT47850.1222555419318624No Hit
CTTCCCATGTACTCTGCGTTGATAC47640.12171899723372884No Hit
GATACCACTGCTTCCCATGTACTCT47500.12136130076830645No Hit
CCACTGCTTCCCATGTACTCTGCGT46670.11924067172330237No Hit
ACTTAGATGTTTCAGTTCCCCCGGT45580.11645574924251385No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43590.11137134948401006No Hit
ATGGGAAGCAGTGGTATCAACGCAG42450.10845867826557072No Hit
ACCCTGTATCGCGCGCCTTTCCAGA41460.10592925326008391No Hit
ATTCCATTCCATTCCATTCCATTCC41130.10508611159158832No Hit
TCATTAACCTATGGATTCAGTTAAT41030.10483061411628661No Hit
GTGGATGCCCTGGCAGTCAGAGGCG40560.10362977598236862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC2900.013.11056918
ATCGCCG2950.012.879456519
CGTCCTG604.1044547E-412.66301810
AAATCGC3100.012.26347117
CCGATGT752.0757254E-411.3997771
ATACCGT855.3430682E-511.1732516
CCGAACA1658.0035534E-1110.94421917
ATGCGTT700.001496028410.85387711
GAAATCG3250.010.81987816
CTTAACG909.480149E-510.56021117
TGTCCCG12850.010.34708211
GTACTGG23200.010.2368691
CCCGAAC1606.1409082E-910.09794316
ACCGTTC856.616757E-410.0556698
CGATGTA951.6500591E-49.997122
AACGTAC1054.089828E-59.95651516
CTAGAAC3250.09.9355993
TACTGGT20950.09.9279272
AGCGTAC11850.09.8605671
GTACAAA7850.09.8023571
Produced by FastQC (version 0.11.5)