FastQCFastQC Report
Wed 25 May 2016
SRR1294630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3913933
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC744191.901386661447705No Hit
GTACATGGGAAGCAGTGGTATCAAC677071.7298967560252054No Hit
GTATCAACGCAGAGTACTTTTTTTT321870.8223697237535746No Hit
GAGTACATGGGAAGCAGTGGTATCA321670.8218587288029714No Hit
CATGTACTCTGCGTTGATACCACTG287230.7338653983090666No Hit
GCGTTGATACCACTGCTTCCCATGT225540.5762490057954492No Hit
GGTATCAACGCAGAGTACTTTTTTT219890.5618133984409034No Hit
TATCAACGCAGAGTACTTTTTTTTT205960.5262226001313768No Hit
ACGCAGAGTACATGGGAAGCAGTGG195250.49885882052656494No Hit
GTATCAACGCAGAGTACATGGGAAG181690.46421336287565473No Hit
TATCAACGCAGAGTACATGGGAAGC157560.40256182208535507No Hit
ACTCTGCGTTGATACCACTGCTTCC157150.40151428243661813No Hit
ACGCAGAGTACTTTTTTTTTTTTTT135600.3464545765091022No Hit
GGTATCAACGCAGAGTACATGGGAA127690.3262447262127379No Hit
GCTTCCCATGTACTCTGCGTTGATA122610.3132654544674117No Hit
CAGTGGTATCAACGCAGAGTACATG104110.2659984215365976No Hit
GTACTGGTTCACTATCGGTCAGTCA100440.2566216641930253No Hit
GTGGTATCAACGCAGAGTACATGGG91020.23255380201960535No Hit
GTACTTTTTTTTTTTTTTTTTTTTT89990.22992217802399786No Hit
ATACCACTGCTTCCCATGTACTCTG82490.2107598673763705No Hit
CATGGGAAGCAGTGGTATCAACGCA79570.20329934109756093No Hit
GCAGTGGTATCAACGCAGAGTACAT78340.20015672215135008No Hit
GGGAAGCAGTGGTATCAACGCAGAG74640.19070331556518724No Hit
GAGTACTTTTTTTTTTTTTTTTTTT74020.18911923121831672No Hit
GGATACCACGTGTCCCGCCCTACTC73430.1876117961140367No Hit
GTACTCTGCGTTGATACCACTGCTT70280.1795636256420332No Hit
GTTAATGATAGTGTGTCGAAACACA70030.17892488195377895No Hit
CTGCTTCCCATGTACTCTGCGTTGA67570.1726396440613572No Hit
ACATGGGAAGCAGTGGTATCAACGC66980.1711322089570772No Hit
GCAGAGTACATGGGAAGCAGTGGTA65190.16655880414917681No Hit
TACCACTGCTTCCCATGTACTCTGC62580.1598903200438025No Hit
AAGCAGTGGTATCAACGCAGAGTAC60140.15365618164644107No Hit
AGCGTACACGGTGGATGCCCTGGCA59790.15276194048288513No Hit
CCATGTACTCTGCGTTGATACCACT56120.1433851831393128No Hit
ATACAGGGTGACAGCCCCGTACACA55360.1414434023270199No Hit
GATACCACTGCTTCCCATGTACTCT54300.1387351290888219No Hit
CTTCCCATGTACTCTGCGTTGATAC53880.13766203969255478No Hit
CCACTGCTTCCCATGTACTCTGCGT53450.13656340054875746No Hit
GTACATGGTAAGCAGTGGTATCAAC51950.132730938419232No Hit
GTTGATACCACTGCTTCCCATGTAC51740.13219439372109845No Hit
ATGGGAAGCAGTGGTATCAACGCAG51270.13099355558718045No Hit
ACTTAGATGTTTCAGTTCCCCCGGT48240.12325198208553903No Hit
ACCCTGTATCGCGCGCCTTTCCAGA47830.12220444243680206No Hit
GTACATGGGGGTTAAGCGACTAAGC46090.11775878636655253No Hit
GTGGATGCCCTGGCAGTCAGAGGCG44100.11267438660804874No Hit
TCATTAACCTATGGATTCAGTTAAT43150.11024716059268261No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42110.10758998684954496No Hit
ATCATTAACTGAATCCATAGGTTAA41300.10552045729960119No Hit
ACCATGTACTCTGCGTTGATACCAC41220.10531605931935985No Hit
ATTCCATTCCATTCCATTCCATTCC40110.10248003734351098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG2000.014.22562319
GACCGTT759.4648385E-713.9615847
GGACCGT1101.8007995E-1013.8461996
AATCGCC2200.013.36295118
AAATCGC2400.013.03965517
GAAATCG2700.011.23956716
CAGTGCG1053.387835E-610.8788789
CCGAACA1053.5435278E-610.83815517
CCCGAAC803.8349553E-410.66880816
GTCCTAT2550.010.4419441
TTAGACA3200.010.4116944
TGTCCCG13450.010.15905611
CGTTATT856.477504E-410.07895910
GTCCGGG856.5365504E-410.0690171
CTAATAC4400.09.7165443
GTCTTAG2600.09.5096271
TACGGCC1002.8050516E-49.48374819
TAACGCC800.00458228879.48374819
CCCGTAC13350.09.44786616
CCGTACA13350.09.44786617
Produced by FastQC (version 0.11.5)