FastQCFastQC Report
Wed 25 May 2016
SRR1294629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010033
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC638832.1223355358562515No Hit
GTACATGGGAAGCAGTGGTATCAAC620842.0625687492462705No Hit
GAGTACATGGGAAGCAGTGGTATCA285840.9496241403333452No Hit
CATGTACTCTGCGTTGATACCACTG257380.8550736819164441No Hit
GCGTTGATACCACTGCTTCCCATGT191200.6352089827586608No Hit
GTATCAACGCAGAGTACATGGGAAG154830.514379742680562No Hit
ACGCAGAGTACATGGGAAGCAGTGG150410.4996955182883377No Hit
ACTCTGCGTTGATACCACTGCTTCC134470.4467392882403615No Hit
TATCAACGCAGAGTACATGGGAAGC133450.4433506210729251No Hit
GTACTGGTTCACTATCGGTCAGTCA129770.4311248414884488No Hit
GTATCAACGCAGAGTACTTTTTTTT125140.4157429503264582No Hit
GGTATCAACGCAGAGTACATGGGAA105950.35198949646067007No Hit
GGATACCACGTGTCCCGCCCTACTC97120.3226542699033532No Hit
GTTAATGATAGTGTGTCGAAACACA94810.31497993543592384No Hit
GCTTCCCATGTACTCTGCGTTGATA90220.29973093318246014No Hit
CAGTGGTATCAACGCAGAGTACATG77190.25644237122981706No Hit
GGTATCAACGCAGAGTACTTTTTTT75930.2522563706112192No Hit
GTGGTATCAACGCAGAGTACATGGG75720.2515587038414529No Hit
TATCAACGCAGAGTACTTTTTTTTT75270.2500637036205251No Hit
ATACAGGGTGACAGCCCCGTACACA74940.248967370125178No Hit
ATACCACTGCTTCCCATGTACTCTG72890.24215681356317356No Hit
GTACTCTGCGTTGATACCACTGCTT64770.2151803651322095No Hit
AGCGTACACGGTGGATGCCCTGGCA64090.21292125368725193No Hit
GCAGTGGTATCAACGCAGAGTACAT63570.21119369787640205No Hit
ACCCTGTATCGCGCGCCTTTCCAGA62550.20780503070896564No Hit
ACTTAGATGTTTCAGTTCCCCCGGT59450.1975061402981296No Hit
GTACATGGGGGTTAAGCGACTAAGC59060.1962104734399922No Hit
GGGAAGCAGTGGTATCAACGCAGAG58640.19481513990045957No Hit
CATGGGAAGCAGTGGTATCAACGCA57990.19265569513689718No Hit
CTGCTTCCCATGTACTCTGCGTTGA55850.18554613853070712No Hit
TACCACTGCTTCCCATGTACTCTGC54860.18225713804466595No Hit
GCAGAGTACATGGGAAGCAGTGGTA54690.18169236018342655No Hit
GTTGATACCACTGCTTCCCATGTAC52870.17564591484545186No Hit
TCATTAACCTATGGATTCAGTTAAT51210.17013102514158485No Hit
AAGCAGTGGTATCAACGCAGAGTAC51140.16989846955166274No Hit
GTATTTAGCCTTGGAGGATGGTCCC50810.16880213605631567No Hit
CCCATATTCAGACAGGATACCACGT50610.16813769151368108No Hit
GTACATGGTAAGCAGTGGTATCAAC50440.1675729136524417No Hit
GTGGATGCCCTGGCAGTCAGAGGCG50290.16707458024546573No Hit
GTTCACTATCGGTCAGTCAGGAGTA47720.15853646787261136No Hit
ATCATTAACTGAATCCATAGGTTAA47200.15680891206176145No Hit
CCACTGCTTCCCATGTACTCTGCGT47190.15677568983462972No Hit
CTATCGGTCAGTCAGGAGTATTTAG47090.15644346756331243No Hit
ACATGGGAAGCAGTGGTATCAACGC46740.1552806896137019No Hit
GATACCACTGCTTCCCATGTACTCT45610.15152657794781652No Hit
CTTCCCATGTACTCTGCGTTGATAC45230.15026413331681082No Hit
GCCCAGAGCCTGAATCAGTGTGTGT44320.14724091064782346No Hit
GTACTTAGATGTTTCAGTTCCCCCG43080.14312135448348906No Hit
TATTCAGACAGGATACCACGTGTCC41960.13940046504473538No Hit
GCCTTGGAGGATGGTCCCCCCATAT41950.13936724281760365No Hit
GTCAGGAGTATTTAGCCTTGGAGGA40780.13548024224319136No Hit
CCATGTACTCTGCGTTGATACCACT40740.13534735333466444No Hit
CCTATGGATTCAGTTAATGATAGTG40490.1345167976563712No Hit
ATGGGAAGCAGTGGTATCAACGCAG40350.134051686476527No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39850.13239057511994054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39470.13112813048893485No Hit
GTATCAACGCAGAGTACATGGGGGT38760.12876935236258208No Hit
ACCATGTACTCTGCGTTGATACCAC38470.12780590777576192No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG37860.12577935192072645No Hit
GGTATCAACGCAGAGTACATGGGGG36820.12232424029902662No Hit
GGTTAATGAGGCGAACCGGGGGAAC34740.11541401705562697No Hit
TTCCCATGTACTCTGCGTTGATACC33190.11026457185020896No Hit
GCCTGAATCAGTGTGTGTGTTAGTG31890.10594568232308417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC801.2899909E-714.24981
AAATCGC3850.012.590939517
ATCGCCG3800.012.49691619
AATCGCC3900.012.43034518
TGTCCCG19500.011.88246811
GTACTAG2100.011.7617411
GAAATCG4200.011.54169516
TAACGCC752.079806E-411.39718619
TCTCGCT1104.991525E-711.2241292
GACCGTT855.344393E-511.1727847
CCGGATC951.35395785E-511.00533713
CGCCCTA22200.011.00344916
GTGTCCC21150.011.00091610
CCCGCCC22450.010.88055514
GACGTAT700.001492279210.8569911
TCCCGCC21800.010.85618213
GTCCCGC21650.010.84305412
GTACAAA5550.010.7836331
CCGAATG5050.010.72904518
CCGCCCT22800.010.67201815
Produced by FastQC (version 0.11.5)