FastQCFastQC Report
Wed 25 May 2016
SRR1294629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010033
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC709502.3571170149961813No Hit
GTACATGGGAAGCAGTGGTATCAAC625972.079611751764848No Hit
GTATCAACGCAGAGTACTTTTTTTT374221.2432421837235672No Hit
GAGTACATGGGAAGCAGTGGTATCA305371.0145071499216123No Hit
CATGTACTCTGCGTTGATACCACTG293270.9743082550922199No Hit
GGTATCAACGCAGAGTACTTTTTTT242940.8071007859382272No Hit
TATCAACGCAGAGTACTTTTTTTTT238590.792649117135925No Hit
GCGTTGATACCACTGCTTCCCATGT206940.6875007682640024No Hit
ACGCAGAGTACATGGGAAGCAGTGG179490.596305754787406No Hit
GTATCAACGCAGAGTACATGGGAAG159160.5287649670286007No Hit
ACGCAGAGTACTTTTTTTTTTTTTT157750.5240806330030269No Hit
ACTCTGCGTTGATACCACTGCTTCC150650.5004928517394992No Hit
TATCAACGCAGAGTACATGGGAAGC148480.4932836284519139No Hit
GTACTGGTTCACTATCGGTCAGTCA127940.42504517392334235No Hit
GGTATCAACGCAGAGTACATGGGAA117730.39112528002184693No Hit
GTACTTTTTTTTTTTTTTTTTTTTT110040.36557738735754725No Hit
GGATACCACGTGTCCCGCCCTACTC101020.3356109384847276No Hit
GCTTCCCATGTACTCTGCGTTGATA98200.32624227043358No Hit
GTTAATGATAGTGTGTCGAAACACA97110.3226210476762215No Hit
CAGTGGTATCAACGCAGAGTACATG96140.3193984916444438No Hit
GAGTACTTTTTTTTTTTTTTTTTTT90610.30102660004059756No Hit
GTGGTATCAACGCAGAGTACATGGG83450.2772394854142795No Hit
ATACAGGGTGACAGCCCCGTACACA82470.27398370715537007No Hit
ATACCACTGCTTCCCATGTACTCTG79380.2637180389716658No Hit
AGCGTACACGGTGGATGCCCTGGCA75620.2512264815701356No Hit
GCAGTGGTATCAACGCAGAGTACAT73190.24315348037712545No Hit
CATGGGAAGCAGTGGTATCAACGCA72690.24149236902053897No Hit
ACCCTGTATCGCGCGCCTTTCCAGA70220.2332864789190019No Hit
GGGAAGCAGTGGTATCAACGCAGAG68220.22664203349265608No Hit
GTACTCTGCGTTGATACCACTGCTT63620.21135980901206067No Hit
GCAGAGTACATGGGAAGCAGTGGTA63440.21076180892368956No Hit
ACTTAGATGTTTCAGTTCCCCCGGT63290.21026347551671362No Hit
CTGCTTCCCATGTACTCTGCGTTGA62180.20657580830509167No Hit
ACATGGGAAGCAGTGGTATCAACGC60650.20149280755393711No Hit
TACCACTGCTTCCCATGTACTCTGC60520.20106091860122463No Hit
AAGCAGTGGTATCAACGCAGAGTAC58560.19454936208340573No Hit
CCCATATTCAGACAGGATACCACGT55270.18361924935706683No Hit
GTGGATGCCCTGGCAGTCAGAGGCG55090.18302124926869573No Hit
TCATTAACCTATGGATTCAGTTAAT54830.18215747136327076No Hit
GTACATGGGGGTTAAGCGACTAAGC54120.179798693236918No Hit
GTTGATACCACTGCTTCCCATGTAC53470.1776392484733556No Hit
CTATCGGTCAGTCAGGAGTATTTAG52930.17584524820824224No Hit
GCAGAGTACTTTTTTTTTTTTTTTT51790.17205791431522513No Hit
CCACTGCTTCCCATGTACTCTGCGT51260.17029713627724347No Hit
ATCATTAACTGAATCCATAGGTTAA50620.1681709137408128No Hit
CTTCCCATGTACTCTGCGTTGATAC50090.16641013570283117No Hit
GATACCACTGCTTCCCATGTACTCT49330.16388524644081975No Hit
GTACATGGTAAGCAGTGGTATCAAC48860.16232380176562847No Hit
CCATGTACTCTGCGTTGATACCACT48330.16056302372764686No Hit
GCCCAGAGCCTGAATCAGTGTGTGT47910.15916769018811422No Hit
ATGGGAAGCAGTGGTATCAACGCAG47200.15680891206176145No Hit
GTATTTAGCCTTGGAGGATGGTCCC46470.15438368948114523No Hit
GTCAGGAGTATTTAGCCTTGGAGGA46250.15365280048424718No Hit
GTTCACTATCGGTCAGTCAGGAGTA46110.15318768930440296No Hit
CCTATGGATTCAGTTAATGATAGTG46050.15298835594161259No Hit
GCCTTGGAGGATGGTCCCCCCATAT44190.14680902169511098No Hit
TATTCAGACAGGATACCACGTGTCC43890.14581235488115912No Hit
ACCATGTACTCTGCGTTGATACCAC43580.1447824658400755No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG42730.14195857653387853No Hit
GGTATCAACGCAGAGTACATGGGGG42300.1405300207672142No Hit
GTACTTAGATGTTTCAGTTCCCCCG41800.13886890941062774No Hit
GTATCAACGCAGAGTACATGGGGGT40370.13411813093079047No Hit
GCCTGAATCAGTGTGTGTGTTAGTG37980.1261780186463072No Hit
ATCAACGCAGAGTACTTTTTTTTTT37250.12375279606569096No Hit
GGTTAATGAGGCGAACCGGGGGAAC36630.12169301798352376No Hit
CACACACACTGATTCAGGCTCTGGG34590.11491568364865101No Hit
TTCCCATGTACTCTGCGTTGATACC33260.11049712744013107No Hit
CGGTACTGGTTCACTATCGGTCAGT31930.10607857123161109No Hit
ATGATAGTGTGTCGAAACACACTGG31540.10478290437347365No Hit
TGATACCACTGCTTCCCATGTACTC31090.10328790415254584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAC500.001476191313.32840310
GACCCGA751.44682235E-512.6943546
GACGGAC550.00301980112.1173387
TGTCCCG18850.011.67715911
GACCGTT907.2876264E-611.6364917
CGCAAAA1157.0836904E-811.5650362
CGAACAC1407.021299E-1011.51483518
AAATCGC3650.011.43132817
GGTCTAC752.0603187E-411.4087441
GAAATCG3750.011.37936716
AATCGCC3700.011.27685118
CACACCG1303.1666787E-810.9854985
GTACTGG28500.010.9750781
ACACCGG700.001465170310.8808756
CCCCGGT11950.010.79214819
ATCGCCG3800.010.73054419
CGCAGCG1605.2386895E-1010.710868
TAGGACT1605.2386895E-1010.710864
CCCCCGG12150.010.69254718
TACTGGT27950.010.67246152
Produced by FastQC (version 0.11.5)