FastQCFastQC Report
Wed 25 May 2016
SRR1294628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4706926
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129640.27542391786061643No Hit
GTACTGGTTCACTATCGGTCAGTCA78990.16781653248850736No Hit
GGTATCAACGCAGAGTACTTTTTTT78650.1670941926854172No Hit
TATCAACGCAGAGTACTTTTTTTTT76420.16235649338867872No Hit
GGATACCACGTGTCCCGCCCTACTC49960.10614146047760258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28050.013.9522721
TCGACCG701.0955317E-412.209839
CCGTGCG700.001496924710.8531839
GTATCAA64600.010.7489351
AATCGCC1951.8189894E-1210.72509818
GTCTAGG3300.010.650461
CGTCTAT856.60425E-410.0578271
TATACTG6700.09.6385815
GCGACCA800.00453194149.4965349
CGCGCCT9550.09.35513212
TGTCCCG12700.09.34688411
CCGTACA12050.09.30811917
CGCCCTA12850.09.17225816
GTACTGG20100.09.0737281
AAATCGC2106.6211214E-109.052690517
GCGCCTT10250.08.99470913
TCGCGCG9650.08.9552819
CCCCGTA12650.08.94119715
CCCGCCC13400.08.93727314
TACACCG1606.7821384E-78.9032855
Produced by FastQC (version 0.11.5)