##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294628_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4706926 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.927317319201535 31.0 30.0 33.0 25.0 34.0 2 30.018275409471066 31.0 30.0 34.0 25.0 34.0 3 29.98495366190163 31.0 30.0 34.0 25.0 34.0 4 33.63782987028052 35.0 35.0 37.0 28.0 37.0 5 34.03257221379729 35.0 35.0 37.0 29.0 37.0 6 34.24510200500284 36.0 35.0 37.0 30.0 37.0 7 34.16000463997097 37.0 35.0 37.0 30.0 37.0 8 33.969029893395394 36.0 35.0 37.0 28.0 37.0 9 35.12043677763364 38.0 35.0 39.0 27.0 39.0 10 35.21357952090175 38.0 35.0 39.0 28.0 39.0 11 35.46833878416614 39.0 35.0 39.0 29.0 39.0 12 35.22852898048535 38.0 35.0 39.0 27.0 39.0 13 35.09801853693897 38.0 35.0 39.0 27.0 39.0 14 35.94839476974994 39.0 35.0 41.0 27.0 41.0 15 35.95107061381462 39.0 35.0 41.0 27.0 41.0 16 36.068262173656436 39.0 35.0 41.0 27.0 41.0 17 36.05236623647791 39.0 35.0 41.0 27.0 41.0 18 35.91882982651522 39.0 35.0 41.0 27.0 41.0 19 36.19504704344194 39.0 35.0 41.0 27.0 41.0 20 36.303030045511655 39.0 35.0 41.0 27.0 41.0 21 36.34387474967739 39.0 36.0 41.0 27.0 41.0 22 36.352463794841896 39.0 36.0 41.0 27.0 41.0 23 36.31798396660581 39.0 35.0 41.0 27.0 41.0 24 36.31874518528653 39.0 35.0 41.0 27.0 41.0 25 35.070198681687366 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.090640458717079 1101 2 1.0446395946289968 1101 3 1.3398164076269339 1101 4 1.1878544255872967 1101 5 0.8473599167887187 1101 6 0.8777025290188547 1101 7 0.959128567520203 1101 8 1.1617078354535764 1101 9 1.3640915543482564 1101 10 1.3544145317704235 1101 11 1.0835330587526997 1101 12 1.3160217421408476 1101 13 1.3699021616539255 1101 14 1.4388577110572243 1101 15 1.6051851789638363 1101 16 1.5066396188923434 1101 17 1.4250812753240822 1101 18 1.55463423675549 1101 19 1.3316425600714936 1101 20 1.2891610534527231 1101 21 1.1511006378232977 1101 22 1.1057549125944277 1101 23 1.1409392793368909 1101 24 1.1921779297057284 1101 25 1.1735986223326051 1102 1 0.8732793374719208 1102 2 0.8512485211251217 1102 3 1.1290311271641968 1102 4 0.9742844145370597 1102 5 0.7884128493933176 1102 6 0.7541941361249016 1102 7 0.6981946895275115 1102 8 0.8225200271834439 1102 9 1.107979164950251 1102 10 1.046926074674488 1102 11 0.8113988082575503 1102 12 1.0356118937820682 1102 13 1.0321850611838457 1102 14 1.2063679694066138 1102 15 1.3407284695600197 1102 16 1.2241296738639278 1102 17 1.1369368749462296 1102 18 1.326653373983575 1102 19 0.9908200747359572 1102 20 0.21670806171569268 1102 21 0.5700623448499158 1102 22 0.6461763225584249 1102 23 0.6935651972722994 1102 24 0.8433733503878784 1102 25 0.8901685192138302 1103 1 0.7901227989891773 1103 2 0.8235199579036312 1103 3 1.1014978180054023 1103 4 0.9636741891384872 1103 5 0.7059287699382679 1103 6 0.5389908880082288 1103 7 0.5688824256001368 1103 8 0.716935383838603 1103 9 0.8177608698031236 1103 10 0.8270649313500087 1103 11 0.6606870545899639 1103 12 0.9360873691343059 1103 13 0.9023072258951075 1103 14 0.747623557767426 1103 15 0.9878518412767434 1103 16 1.02228199101053 1103 17 1.043415968312054 1103 18 1.1441868549025287 1103 19 0.8701103161286454 1103 20 0.04005364206863504 1103 21 0.4241890867891698 1103 22 -0.1827892981449466 1103 23 0.4749644371795583 1103 24 0.6365433378109557 1103 25 0.7081912042862939 1104 1 0.41759750319537403 1104 2 0.40702165551601155 1104 3 0.7438099746261173 1104 4 0.5835053214182011 1104 5 0.42186041002826613 1104 6 0.3020770767860128 1104 7 0.3396652581245334 1104 8 0.47596394343108983 1104 9 0.8076075731278181 1104 10 0.7336106355764045 1104 11 0.5439790475612085 1104 12 0.5424557841548037 1104 13 0.5499177428756568 1104 14 0.8696571705823857 1104 15 0.9390726894391648 1104 16 0.7243203793432826 1104 17 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0.32666355072570497 2207 9 -1.3820302737247232 2207 10 -0.33559325281535735 2207 11 0.07810535423880083 2207 12 0.5173159260543017 2207 13 0.6337554047575509 2207 14 0.9097870472599396 2207 15 0.8145240058609247 2207 16 0.7865261502440788 2207 17 0.7344861512878609 2207 18 0.8389052807168724 2207 19 -0.4077358833021023 2207 20 0.23323127695376655 2207 21 -0.38499703368088234 2207 22 0.23448561305806237 2207 23 -0.6661955245581268 2207 24 -1.7049327315099703 2207 25 -0.3481060380573169 2208 1 0.5154845085690489 2208 2 0.16860674310155943 2208 3 0.7028503946703495 2208 4 0.5890069763594568 2208 5 0.4624397124022366 2208 6 0.3722008702847788 2208 7 0.34286624921383435 2208 8 0.5365310292159435 2208 9 -0.11649506023125866 2208 10 0.037285960544814145 2208 11 0.30850928016100454 2208 12 0.28959081133797326 2208 13 0.5626729809809845 2208 14 0.8547981909615316 2208 15 0.8101257355681213 2208 16 0.5404347981594668 2208 17 0.5738979600495782 2208 18 0.6679146436025007 2208 19 -1.503493740446963 2208 20 -0.10674974598168063 2208 21 -0.3071079704905699 2208 22 0.13484903196288656 2208 23 0.3735967818714343 2208 24 0.5299372186827824 2208 25 0.5786580753215276 2209 1 0.10692419728500369 2209 2 -1.4598393523008397 2209 3 -0.27098901772655637 2209 4 0.16412280251532252 2209 5 0.0921924434373338 2209 6 0.1407775716213422 2209 7 0.14101455496815873 2209 8 0.3248701299145438 2209 9 -2.1959362641751596 2209 10 -0.408675976180767 2209 11 0.03982220655376523 2209 12 0.25956109726855914 2209 13 0.48611074772441754 2209 14 0.6382068291138765 2209 15 0.7830206778705247 2209 16 0.7034601751791172 2209 17 0.4813816718920094 2209 18 0.6486650806649976 2209 19 0.36413052712048 2209 20 0.3504152058988552 2209 21 0.36345502246798844 2209 22 0.25260367494805536 2209 23 0.4063647137949218 2209 24 0.44750740258478316 2209 25 0.508066217696495 2210 1 0.1708125200891608 2210 2 0.16312777093855502 2210 3 0.0768301953295989 2210 4 0.283217698410958 2210 5 0.1242481826333588 2210 6 0.06883836036705304 2210 7 0.09815619452093927 2210 8 0.25064554779311976 2210 9 0.33888537840954314 2210 10 -1.7029241970032345 2210 11 -0.5662361642990703 2210 12 -2.432966635527187 2210 13 -0.8439091476143687 2210 14 -0.6332454873990443 2210 15 -0.8953063184535992 2210 16 -0.1923285547406195 2210 17 0.006979009949205306 2210 18 0.31003817403529155 2210 19 0.2091219183846107 2210 20 0.24558934372041108 2210 21 0.25267622525749545 2210 22 0.1834603497186933 2210 23 0.10574477759830359 2210 24 0.16499539955685094 2210 25 0.062326893675908934 2211 1 -0.30089283267312084 2211 2 -0.015293681345699639 2211 3 -1.4187464478760319 2211 4 -0.6097715318966266 2211 5 -0.47202848631250305 2211 6 -0.43895590973897924 2211 7 -0.47694036526209516 2211 8 -0.03271560641957194 2211 9 0.1127701648040329 2211 10 0.13197077889454079 2211 11 -0.22886776951679622 2211 12 -0.25707784864174243 2211 13 -0.2627072099130814 2211 14 -0.8153285165036195 2211 15 -0.3394592739377913 2211 16 -0.09152273868701144 2211 17 -0.20497210720153447 2211 18 -0.1168274472770463 2211 19 -0.10687826639011178 2211 20 -0.027842773045840374 2211 21 -0.11403804577372512 2211 22 -0.2574640810295321 2211 23 -0.4618373219322933 2211 24 -0.23312657018635718 2211 25 -0.052916023269276025 2212 1 -0.39469140160079164 2212 2 -0.35484132897158105 2212 3 -2.556868897490446 2212 4 -1.5579414097473077 2212 5 -0.7063341189277637 2212 6 -0.5018684407294458 2212 7 -0.36831220688164024 2212 8 -0.4335781381129209 2212 9 -0.9282684289249872 2212 10 -0.22341230648017785 2212 11 -0.37570690503497417 2212 12 -0.045004319172797125 2212 13 -0.41151031767839186 2212 14 -0.007800273268586011 2212 15 0.10985914070229796 2212 16 -1.164517429368587 2212 17 -1.387361608563488 2212 18 -0.3074360044590776 2212 19 -0.17739246360507366 2212 20 -0.22360666016921016 2212 21 -0.08470587853034317 2212 22 -0.38260578398944745 2212 23 0.07062872202877912 2212 24 -0.1169653829759767 2212 25 0.14304504232717363 2213 1 -3.270453520106301 2213 2 -1.5170586143052383 2213 3 -2.5983556158304424 2213 4 -1.2412624050644112 2213 5 -0.9073686956505469 2213 6 -0.6833629554846041 2213 7 -0.45882574842215007 2213 8 -0.22084454843144385 2213 9 -0.49250649965285476 2213 10 -0.1945683710314654 2213 11 -0.5515823995648717 2213 12 -0.2620278673743286 2213 13 -0.6870156338747506 2213 14 -0.30015667036599325 2213 15 -0.39382272266615814 2213 16 -1.7070149693346792 2213 17 -1.1236375966140315 2213 18 -0.39172526912339833 2213 19 -0.28743237923222154 2213 20 -0.21629774803405155 2213 21 -0.14996736121170073 2213 22 -0.0868277194179754 2213 23 -0.028771975863918442 2213 24 -0.011409970396748292 2213 25 -0.0975710763196318 2214 1 -0.7277827624219562 2214 2 -0.4990048836156369 2214 3 -6.452223668068058 2214 4 -2.0580748951105967 2214 5 -1.2383529288086947 2214 6 -0.6869040249224128 2214 7 -0.5726986282597011 2214 8 -0.34142007797436236 2214 9 -0.14226661272818575 2214 10 -0.021195321979064374 2214 11 -0.6661731982589316 2214 12 -0.3368444683458165 2214 13 -5.178381944194868 2214 14 -3.778699979412025 2214 15 -1.9410249543113238 2214 16 -2.717325876724388 2214 17 -3.278339778490121 2214 18 -0.9491114970259318 2214 19 -0.6217362094599608 2214 20 -0.2679493346720534 2214 21 -0.2620734390107131 2214 22 -0.22194950433595295 2214 23 -0.12426513759348978 2214 24 -0.012656685638035015 2214 25 0.31264303022599904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1750.0 3 229.0 4 255.0 5 465.0 6 73.0 7 72.0 8 170.0 9 407.0 10 1058.0 11 2604.0 12 5611.0 13 9889.0 14 15458.0 15 20757.0 16 25319.0 17 28455.0 18 29578.0 19 30340.0 20 30706.0 21 31812.0 22 32655.0 23 34493.0 24 38168.0 25 42131.0 26 47637.0 27 55287.0 28 65311.0 29 80582.0 30 100274.0 31 125485.0 32 165257.0 33 219513.0 34 295757.0 35 400421.0 36 554200.0 37 821331.0 38 1393416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24392185834289 20.591219388127254 13.469681223328683 23.69517753020118 2 15.420804596064192 21.17332444547796 40.29072299628423 23.115147962173616 3 18.19844297762673 26.373320601892203 30.04491149069247 25.383324929788596 4 12.374243270283948 17.999459679944326 38.305014710011584 31.32128233976014 5 12.930821016216107 38.65101077269076 35.16543964876475 13.252728562328379 6 29.981678666169483 38.90636646154199 17.96864071349881 13.143314158789712 7 26.198524062743278 33.80053205850177 22.646457457216144 17.3544864215388 8 24.303058930369243 37.15874653070665 21.19102626459144 17.347168274332674 9 25.11588276261445 17.163701887713408 21.49819766270144 36.2222176869707 10 15.014017178617356 30.17365909906968 33.53161683217928 21.28070689013368 11 32.4975548430476 25.714526836354857 23.741161350173048 18.046756970424497 12 21.082031132915958 27.25453445072896 31.100178927003437 20.563255489351643 13 26.93824779070714 23.725757088433575 26.89005585969877 22.445939261160515 14 20.609934538871386 23.46473107110886 28.157681537019563 27.76765285300019 15 22.487195246004553 30.785853576923234 24.4786206899484 22.248330487123816 16 21.51744923770193 30.616706890178015 25.804298392200387 22.06154547991967 17 20.57706391495721 29.84186811820191 27.749131853099023 21.83193611374186 18 20.91436658746315 29.268756084940666 28.867426589233315 20.94945073836287 19 21.660599164751172 28.95374071910315 28.914708015820572 20.4709521003251 20 22.379803215791718 28.0325023022783 28.662493229218484 20.9252012527115 21 22.59547706539425 28.06026809724424 28.26774606227655 21.076508775084957 22 22.366330114928452 27.938279927455678 28.380297724241863 21.315092233374003 23 22.271235862281262 28.005342793839844 28.310535967342886 21.41288537653601 24 22.097454721728788 27.88629113101863 28.325291304296986 21.690962842955596 25 21.983723099222935 28.169846808516063 28.420988404711927 21.42544168754907 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3720.0 1 3720.0 2 3576.0 3 3432.0 4 3432.0 5 3432.0 6 6012.0 7 8592.0 8 8592.0 9 8592.0 10 13477.0 11 18362.0 12 18362.0 13 18362.0 14 30016.0 15 41670.0 16 41670.0 17 41670.0 18 68001.5 19 94333.0 20 94333.0 21 94333.0 22 135922.5 23 177512.0 24 177512.0 25 177512.0 26 231021.5 27 284531.0 28 284531.0 29 284531.0 30 339501.0 31 394471.0 32 394471.0 33 394471.0 34 435356.0 35 476241.0 36 476241.0 37 476241.0 38 496940.0 39 517639.0 40 517639.0 41 517639.0 42 529327.5 43 541016.0 44 541016.0 45 541016.0 46 547912.0 47 554808.0 48 554808.0 49 554808.0 50 522738.0 51 490668.0 52 490668.0 53 490668.0 54 447270.0 55 403872.0 56 403872.0 57 403872.0 58 347977.0 59 292082.0 60 292082.0 61 292082.0 62 248312.0 63 204542.0 64 204542.0 65 204542.0 66 156924.0 67 109306.0 68 109306.0 69 109306.0 70 82226.0 71 55146.0 72 55146.0 73 55146.0 74 39149.5 75 23153.0 76 23153.0 77 23153.0 78 15686.0 79 8219.0 80 8219.0 81 8219.0 82 5389.5 83 2560.0 84 2560.0 85 2560.0 86 1682.0 87 804.0 88 804.0 89 804.0 90 488.0 91 172.0 92 172.0 93 172.0 94 107.5 95 43.0 96 43.0 97 43.0 98 37.5 99 32.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09651734486584239 2 0.0505425409279857 3 0.0544304286916769 4 0.04901288016850063 5 0.045868577496225776 6 0.06628529957768615 7 0.06229118537236405 8 0.05944431673665573 9 0.06445820478163455 10 0.06490435583648436 11 0.057978391842149216 12 0.058913184528501195 13 0.05989046779150554 14 0.061887524894166594 15 0.06392707257348001 16 0.059380580871677184 17 0.062482392967299676 18 0.14442547004138157 19 0.06751752630060469 20 0.061398883262664426 21 0.059741750773222274 22 0.06464941237657018 23 0.06416077074506801 24 0.07459220731322311 25 0.06809114908541158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4706926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.84815957837428 #Duplication Level Percentage of deduplicated Percentage of total 1 73.32785131481629 41.68553393083979 2 14.859344242463775 16.894527454511557 3 5.4545434374964685 9.302422672859203 4 2.4662003056973383 5.607957941220709 5 1.2532714222275592 3.562308690290419 6 0.7389442694170291 2.5204573048406678 7 0.4660508620061907 1.854589364247677 8 0.28960835665143037 1.3170961659320994 9 0.21709851509947764 1.1107485927542884 >10 0.8670045554058277 8.260363993578114 >50 0.034754452064693214 1.3551168109696705 >100 0.020519430058899876 2.3188185452010712 >500 0.002499081400672979 1.0198125283920227 >1k 0.002158295837347729 2.4073648698853547 >5k 1.1359451775500785E-4 0.5038250160455335 >10k+ 3.786483925166928E-5 0.2790561184317823 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12964 0.27542391786061643 No Hit GTACTGGTTCACTATCGGTCAGTCA 7899 0.16781653248850736 No Hit GGTATCAACGCAGAGTACTTTTTTT 7865 0.1670941926854172 No Hit TATCAACGCAGAGTACTTTTTTTTT 7642 0.16235649338867872 No Hit GGATACCACGTGTCCCGCCCTACTC 4996 0.10614146047760258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 2.124528832618146E-5 0.0 11 0.0 0.0 4.249057665236292E-5 4.249057665236292E-5 0.0 12 0.0 0.0 4.249057665236292E-5 4.249057665236292E-5 0.0 13 0.0 2.124528832618146E-5 4.249057665236292E-5 4.249057665236292E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2805 0.0 13.952272 1 TCGACCG 70 1.0955317E-4 12.20983 9 CCGTGCG 70 0.0014969247 10.853183 9 GTATCAA 6460 0.0 10.748935 1 AATCGCC 195 1.8189894E-12 10.725098 18 GTCTAGG 330 0.0 10.65046 1 CGTCTAT 85 6.60425E-4 10.057827 1 TATACTG 670 0.0 9.638581 5 GCGACCA 80 0.0045319414 9.496534 9 CGCGCCT 955 0.0 9.355132 12 TGTCCCG 1270 0.0 9.346884 11 CCGTACA 1205 0.0 9.308119 17 CGCCCTA 1285 0.0 9.172258 16 GTACTGG 2010 0.0 9.073728 1 AAATCGC 210 6.6211214E-10 9.0526905 17 GCGCCTT 1025 0.0 8.994709 13 TCGCGCG 965 0.0 8.955281 9 CCCCGTA 1265 0.0 8.941197 15 CCCGCCC 1340 0.0 8.937273 14 TACACCG 160 6.7821384E-7 8.903285 5 >>END_MODULE