FastQCFastQC Report
Wed 25 May 2016
SRR1294628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4706926
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT336650.7152226315008989No Hit
GGTATCAACGCAGAGTACTTTTTTT219170.4656329842449191No Hit
TATCAACGCAGAGTACTTTTTTTTT208840.44368660140397365No Hit
ACGCAGAGTACTTTTTTTTTTTTTT114000.24219628691846867No Hit
GTACTGGTTCACTATCGGTCAGTCA79100.16805023066009536No Hit
GTACTTTTTTTTTTTTTTTTTTTTT69530.1477184897319397No Hit
GAGTACTTTTTTTTTTTTTTTTTTT60380.12827905091348366No Hit
GGATACCACGTGTCCCGCCCTACTC55260.11740146329047875No Hit
ATACAGGGTGACAGCCCCGTACACA48350.10272096905708736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45000.015.323951
AAATCGC2250.010.95816117
CCGATAC700.001490814810.8585043
GTATCAA104450.010.8454741
CCGTACA12150.010.77084217
CCCCGTA11950.010.71292615
CGGGTCT1105.9059457E-610.3849436
CCCGTAC12550.010.27642416
CGCCCTA11950.010.15754916
CTACGCT750.002604519710.1541674
TGTCCCG12250.09.91437411
AATCGCC2700.09.83424818
TCCCGCC12850.09.51992213
GTCCCGC12850.09.5197212
ATCGCCG2700.09.48302519
CCCGCCC13000.09.48302514
GTCCTAT2600.09.1455931
TACACCG1151.0869706E-49.1056395
GCCCCGT12050.09.05018914
GTACTGG23050.09.0368781
Produced by FastQC (version 0.11.5)