##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294628_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4706926 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48267531718153 31.0 31.0 34.0 29.0 34.0 2 31.57414903059874 31.0 31.0 34.0 28.0 34.0 3 31.728179495492387 33.0 31.0 34.0 29.0 34.0 4 35.33100520382092 37.0 35.0 37.0 33.0 37.0 5 35.48986684727994 37.0 35.0 37.0 33.0 37.0 6 35.11562556963929 37.0 35.0 37.0 32.0 37.0 7 34.95500524121263 37.0 35.0 37.0 32.0 37.0 8 34.998358801476805 37.0 35.0 37.0 32.0 37.0 9 36.462444278920046 39.0 37.0 39.0 32.0 39.0 10 36.23409482112105 39.0 37.0 39.0 32.0 39.0 11 36.4082469110413 39.0 37.0 39.0 32.0 39.0 12 36.36001288314284 39.0 37.0 39.0 32.0 39.0 13 36.23992304106757 39.0 37.0 39.0 31.0 39.0 14 37.34050694657192 40.0 37.0 41.0 31.0 41.0 15 37.39652716018905 40.0 37.0 41.0 31.0 41.0 16 37.44288862837444 40.0 37.0 41.0 32.0 41.0 17 37.49498844893674 40.0 37.0 41.0 32.0 41.0 18 37.493320481350246 40.0 37.0 41.0 32.0 41.0 19 37.509405714047766 40.0 37.0 41.0 31.0 41.0 20 37.55832745192935 40.0 37.0 41.0 32.0 41.0 21 37.43878573829289 40.0 37.0 41.0 31.0 41.0 22 37.43676042495675 40.0 37.0 41.0 31.0 41.0 23 37.43478525050107 40.0 37.0 41.0 31.0 41.0 24 37.34133678753395 40.0 37.0 41.0 31.0 41.0 25 36.3014491836073 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5179668554638539 1101 2 0.43518567329210356 1101 3 0.3558934249704713 1101 4 0.30597361958825786 1101 5 0.3320070993626416 1101 6 0.39055889154214896 1101 7 0.4414575484130623 1101 8 0.4039793687203712 1101 9 0.6621329922727384 1101 10 0.7064441837601336 1101 11 0.6267380149779314 1101 12 0.724923142246638 1101 13 0.7477992854582425 1101 14 0.9151371053386512 1101 15 0.8766512382178888 1101 16 0.8785415415711881 1101 17 0.8661673775067698 1101 18 0.9549432497581947 1101 19 1.0436147772426807 1101 20 0.9090257092536831 1101 21 1.1620293690185548 1101 22 1.161876590624395 1101 23 1.055784869582908 1101 24 1.1717911274104011 1101 25 1.0533313755352296 1102 1 0.3373814124666268 1102 2 0.36308342936209925 1102 3 0.2624932768082786 1102 4 0.2657393437944293 1102 5 0.28822448500999087 1102 6 0.31280290248226095 1102 7 0.349007651870302 1102 8 0.410799771544454 1102 9 0.5726317342675671 1102 10 0.6613714718340589 1102 11 0.6000706599452883 1102 12 0.573610695261344 1102 13 0.6356789878634075 1102 14 0.7392860464793145 1102 15 0.7733952508135147 1102 16 0.723242584923895 1102 17 0.6602437471333005 1102 18 0.6652571752996366 1102 19 0.8125553243734558 1102 20 0.7989576088482337 1102 21 1.0554979082205236 1102 22 1.0091928401367838 1102 23 0.9140209742882774 1102 24 1.0094530073583172 1102 25 0.9320197244219912 1103 1 0.3022964698627426 1103 2 0.299971565335607 1103 3 0.2695540965990837 1103 4 0.19356968992237 1103 5 0.19836014790750767 1103 6 0.23553465047336175 1103 7 0.2800892135616806 1103 8 0.31070624033220895 1103 9 0.32417371407122175 1103 10 0.5113144846969746 1103 11 0.5227038200090632 1103 12 0.48316912392256484 1103 13 0.5805144182120827 1103 14 0.7319514674244232 1103 15 0.6432324738337485 1103 16 0.6484659241743458 1103 17 0.5790174564908597 1103 18 0.6228203575132625 1103 19 0.6965463409569566 1103 20 0.6773084422993776 1103 21 0.9139208761327282 1103 22 0.844903422111841 1103 23 0.8375794051115122 1103 24 0.9506479511717245 1103 25 0.9616368566207427 1104 1 0.18255121270730612 1104 2 0.19094473459786343 1104 3 0.11841037758987127 1104 4 0.12101533234043416 1104 5 0.14293954498230477 1104 6 0.12629829956873095 1104 7 0.21154163649602964 1104 8 0.19664446899068366 1104 9 -0.011486580492523046 1104 10 0.39795709749930097 1104 11 0.3540567829468131 1104 12 0.3739765265238333 1104 13 -0.06924950442547129 1104 14 0.3248902286458346 1104 15 0.4194171378467928 1104 16 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0.022057132179554628 2206 25 -0.007951298040168808 2207 1 0.21258869932452384 2207 2 0.23941799433297462 2207 3 0.2098770226158173 2207 4 0.1937550700509476 2207 5 0.1568291266198969 2207 6 0.3288943143149794 2207 7 0.25926342490028986 2207 8 0.2803257793614833 2207 9 0.3749160145606183 2207 10 0.6520661158652459 2207 11 0.45533818681847293 2207 12 0.3721696656424811 2207 13 0.3931241078790393 2207 14 0.3878301034801481 2207 15 0.4351633684026126 2207 16 0.5592925952959362 2207 17 0.5476432301513299 2207 18 0.49594779374508136 2207 19 0.6094576315772215 2207 20 0.5355019401548518 2207 21 0.90669694627897 2207 22 0.7592952369805843 2207 23 0.670658067505002 2207 24 0.8221907228749146 2207 25 0.8735109163993044 2208 1 0.15220517695589209 2208 2 0.13627414600735221 2208 3 0.15012298740738217 2208 4 0.12924865012777786 2208 5 0.09465118648193993 2208 6 0.19653058545908664 2208 7 0.22152790225486285 2208 8 0.08994273390604235 2208 9 0.2773594040719445 2208 10 0.3015931089394641 2208 11 -0.010282747783470825 2208 12 -0.24787709990418705 2208 13 0.007747749834713602 2208 14 -0.45796424474996655 2208 15 0.1646345719844433 2208 16 0.20282892957791177 2208 17 0.1724861399610944 2208 18 0.3017596228414945 2208 19 0.4267288090945982 2208 20 0.40158135750805 2208 21 0.6601164606317198 2208 22 0.6990082874083257 2208 23 0.6214413060380224 2208 24 0.6484907342579262 2208 25 0.6055243393379115 2209 1 0.036707674050216355 2209 2 0.009569451924377859 2209 3 0.08210537611889279 2209 4 -0.4782072118216121 2209 5 -0.04968323322968615 2209 6 0.07709496819788342 2209 7 0.1451961645518267 2209 8 0.14978375607604733 2209 9 0.1907516655350392 2209 10 0.37074181676122464 2209 11 0.30098405193683675 2209 12 0.28544705731603415 2209 13 0.334093247531392 2209 14 0.41854071018407524 2209 15 -0.215789767844889 2209 16 -0.21687799967926225 2209 17 0.15513564406679592 2209 18 -0.22842475021560915 2209 19 0.2596779344517728 2209 20 0.2882364688424701 2209 21 0.5739120423332622 2209 22 0.4976413875971204 2209 23 0.45836045056147157 2209 24 0.6200269450488349 2209 25 0.6684561818089918 2210 1 -0.173297691657325 2210 2 -0.10519950848807014 2210 3 -0.04890658917621593 2210 4 0.03819189808263701 2210 5 -0.04423285538613442 2210 6 -0.44033506234409714 2210 7 -0.17908482980620022 2210 8 -0.26012035434183645 2210 9 0.02389366130564241 2210 10 0.2086163732342996 2210 11 0.10361049762766328 2210 12 0.11501187316821415 2210 13 0.18163015006866345 2210 14 0.1525642245496428 2210 15 0.06421512163117882 2210 16 0.10621935583144193 2210 17 -0.8840568352325135 2210 18 -1.4055591731576982 2210 19 -0.34906495241191493 2210 20 -0.11979471376412221 2210 21 -0.3922423194982727 2210 22 -0.25563453227763944 2210 23 -0.30459695190415914 2210 24 0.20952041843356284 2210 25 0.1329033618371227 2211 1 -0.2617037538525757 2211 2 -0.17621787302562808 2211 3 -0.11428754119975792 2211 4 -0.07335607142935885 2211 5 -0.10465216621798845 2211 6 -0.10723753706717076 2211 7 -0.1773928522043775 2211 8 -0.10492337155291409 2211 9 -0.012575144906747937 2211 10 0.06017336178354071 2211 11 -0.2923816611317207 2211 12 -0.12868383270789252 2211 13 -0.1385783753417229 2211 14 -0.170185642618911 2211 15 -0.22898194960628615 2211 16 -0.153352752822137 2211 17 -0.3828342016241848 2211 18 -0.42188859568132386 2211 19 -0.22934578898626512 2211 20 -0.30067288868526276 2211 21 0.0031195832664963064 2211 22 -0.05335024708568881 2211 23 -0.7968982530451427 2211 24 -0.14904190422761587 2211 25 -0.7344732228594282 2212 1 -0.16702705219224612 2212 2 -0.23577192138466074 2212 3 -0.1542560160934805 2212 4 -0.07148001056017961 2212 5 -0.07971437147394766 2212 6 -0.14241143212565532 2212 7 -0.22454878812167323 2212 8 -0.35284360286463823 2212 9 -0.1314669088082283 2212 10 -0.45942433718280284 2212 11 -0.1692472639805871 2212 12 -0.2500553124168192 2212 13 -0.20714585237909233 2212 14 -0.27337645595169846 2212 15 -0.11336324546761034 2212 16 -0.3070342361890539 2212 17 -0.5011698674365164 2212 18 -0.3427443437939246 2212 19 -0.4155896204292375 2212 20 -0.5984076213664196 2212 21 -0.07647218156218827 2212 22 -0.3167811178216837 2212 23 -0.22715359743300212 2212 24 -0.45673616573106557 2212 25 -0.21216577650370283 2213 1 -0.20629437840031173 2213 2 -0.26061556415241327 2213 3 -0.18104013360992965 2213 4 -0.12064916249187263 2213 5 -0.13887118538941223 2213 6 -0.35891574916978186 2213 7 -0.42372309981680445 2213 8 -0.3375395174421669 2213 9 -0.36658931757250457 2213 10 -0.3538821961711349 2213 11 -0.11573008411299668 2213 12 -0.04201226308493489 2213 13 0.00872885998617079 2213 14 -0.7470655606507393 2213 15 -0.30785562062404637 2213 16 -0.43476727078596156 2213 17 -0.3701936124407581 2213 18 -0.3189725062621349 2213 19 -0.3661556426931796 2213 20 -0.4179834610579931 2213 21 -0.13972825721517523 2213 22 -0.47468876436401075 2213 23 -0.5741725157245554 2213 24 -0.7564074799694893 2213 25 -0.5709496492783188 2214 1 -0.150026710379926 2214 2 -0.09926672277639881 2214 3 -0.12371860550035763 2214 4 -0.061552122756246774 2214 5 -0.03921484082817983 2214 6 -0.011150629325854311 2214 7 -0.054070786550362016 2214 8 -0.13080874821140043 2214 9 -0.13945945015731098 2214 10 -0.002460293798144164 2214 11 0.12595723671834236 2214 12 -0.013969879743640945 2214 13 -0.05661793090409617 2214 14 -0.10308736945787444 2214 15 0.09160338956866099 2214 16 -0.08414416201068065 2214 17 -0.24396556060129626 2214 18 -0.20969331172479144 2214 19 -0.3537743419287267 2214 20 -0.3301982842782536 2214 21 -0.44182154436280996 2214 22 -0.6250946311421615 2214 23 -0.9066473325743729 2214 24 -0.3228865172365687 2214 25 -0.7185385866091067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 25.0 9 60.0 10 117.0 11 294.0 12 600.0 13 1515.0 14 3105.0 15 5291.0 16 8198.0 17 10875.0 18 13043.0 19 14792.0 20 16244.0 21 17660.0 22 19308.0 23 21621.0 24 24209.0 25 27942.0 26 32646.0 27 36881.0 28 44405.0 29 56067.0 30 72414.0 31 96621.0 32 131673.0 33 180526.0 34 252612.0 35 358896.0 36 520742.0 37 838869.0 38 1899670.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.402258451543155 20.809167181639438 12.631541341503716 25.157033025313698 2 15.218021273332107 21.44397426260791 39.36579840005982 23.972206064000158 3 17.624144275460495 26.38778890841022 30.485204668696515 25.50286214743277 4 12.288394619771656 17.75828918546736 38.500211310565334 31.45310488419565 5 12.898965502146472 38.21938528531885 35.28929262208932 13.592356590445357 6 30.00105605797992 39.145306990955085 17.552979794019823 13.30065715704517 7 26.272293262306 33.81391110618102 22.577762647493707 17.336032984019276 8 24.585553412153303 36.97485759408268 20.966426500781782 17.473162492982237 9 25.33291975742073 16.346333001551972 21.593655292266803 36.727091948760496 10 14.98388979248978 29.916151002833498 33.54183802444009 21.55812118023664 11 32.804373970386244 25.02575030926945 23.803915407603213 18.365960312741095 12 21.395546668286997 26.78017904937859 31.438594493396653 20.385679788937757 13 27.40916463030405 22.877222099707623 27.216618418575877 22.496994851412452 14 20.85477708413712 22.804621652352893 28.741856615241325 27.598744648268664 15 22.744024847101056 30.04890514221031 25.00308591681048 22.203984093878155 16 21.423108349787825 30.087475424491846 26.331687671901083 22.157728553819247 17 20.466716593104838 29.283938027480787 28.285154320495863 21.96419105891851 18 20.66564717219882 28.468157864993326 29.817515022600826 21.048679940207034 19 21.421580177029355 28.198732797340416 30.139010725311316 20.240676300318913 20 22.069852986479038 27.348916933685214 29.90058879342176 20.680641286413987 21 22.250336898090104 27.477945736409403 29.377343494557902 20.89437387094259 22 21.95776014508411 27.431196695325283 29.55648938437197 21.054553775218636 23 21.983080230809108 27.440789305958035 29.484758610726335 21.091371852506523 24 21.72226629439256 27.377443367497172 29.502120917133603 21.39816942097666 25 21.65398796050515 27.65064134296414 29.547008376316857 21.148362320213852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1353.0 1 1353.0 2 1869.5 3 2386.0 4 2386.0 5 2386.0 6 6674.0 7 10962.0 8 10962.0 9 10962.0 10 14777.5 11 18593.0 12 18593.0 13 18593.0 14 27177.0 15 35761.0 16 35761.0 17 35761.0 18 61967.0 19 88173.0 20 88173.0 21 88173.0 22 131658.0 23 175143.0 24 175143.0 25 175143.0 26 232696.0 27 290249.0 28 290249.0 29 290249.0 30 347419.0 31 404589.0 32 404589.0 33 404589.0 34 444097.5 35 483606.0 36 483606.0 37 483606.0 38 497991.5 39 512377.0 40 512377.0 41 512377.0 42 524781.5 43 537186.0 44 537186.0 45 537186.0 46 546480.0 47 555774.0 48 555774.0 49 555774.0 50 523771.0 51 491768.0 52 491768.0 53 491768.0 54 448110.5 55 404453.0 56 404453.0 57 404453.0 58 348636.0 59 292819.0 60 292819.0 61 292819.0 62 250064.5 63 207310.0 64 207310.0 65 207310.0 66 157223.5 67 107137.0 68 107137.0 69 107137.0 70 80908.0 71 54679.0 72 54679.0 73 54679.0 74 38326.5 75 21974.0 76 21974.0 77 21974.0 78 14783.0 79 7592.0 80 7592.0 81 7592.0 82 4908.5 83 2225.0 84 2225.0 85 2225.0 86 1436.5 87 648.0 88 648.0 89 648.0 90 398.0 91 148.0 92 148.0 93 148.0 94 83.0 95 18.0 96 18.0 97 18.0 98 10.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09373421209511261 2 0.0 3 0.1292775794648142 4 0.012386003094163793 5 0.0226899679323618 6 0.015827739803005187 7 0.024049666385237416 8 0.021500231786095638 9 2.3369817158799605E-4 10 0.37525552770534315 11 0.06583914852283634 12 0.0014446796061803394 13 8.498115330472584E-5 14 8.073209563948955E-4 15 0.0012534720112447064 16 8.498115330472584E-5 17 2.124528832618146E-5 18 3.824151898712663E-4 19 4.886416315021736E-4 20 2.5494345991417753E-4 21 2.76188748240359E-4 22 0.002953095077339223 23 1.2747172995708877E-4 24 0.0 25 0.0017846042193992429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4706926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.65537037448147 #Duplication Level Percentage of deduplicated Percentage of total 1 71.22536848779313 38.216235262714626 2 15.889678900749294 17.051332131025738 3 6.001883994798688 9.660999260567882 4 2.635100752083188 5.655492273083927 5 1.401246416205 3.759219772369705 6 0.7744738208348795 2.493280782134116 7 0.4853050240908203 1.8227454568532733 8 0.3179877040728793 1.364939842924908 9 0.24702729173190457 1.1928906747432384 >10 0.9494390751989389 8.5884014691322 >50 0.04435398856247557 1.6323623486569139 >100 0.022481930151363246 2.4482263806417217 >500 0.002905922119863719 1.1125518271032884 >1k 0.002428234609550163 2.5601771657234007 >5k 1.5922849898666842E-4 0.5644453135878541 >10k+ 1.5922849898666842E-4 1.876700038737291 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 33665 0.7152226315008989 No Hit GGTATCAACGCAGAGTACTTTTTTT 21917 0.4656329842449191 No Hit TATCAACGCAGAGTACTTTTTTTTT 20884 0.44368660140397365 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11400 0.24219628691846867 No Hit GTACTGGTTCACTATCGGTCAGTCA 7910 0.16805023066009536 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6953 0.1477184897319397 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6038 0.12827905091348366 No Hit GGATACCACGTGTCCCGCCCTACTC 5526 0.11740146329047875 No Hit ATACAGGGTGACAGCCCCGTACACA 4835 0.10272096905708736 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.124528832618146E-5 0.0 10 0.0 0.0 0.0 4.249057665236292E-5 0.0 11 0.0 0.0 2.124528832618146E-5 4.249057665236292E-5 0.0 12 0.0 0.0 6.373586497854438E-5 4.249057665236292E-5 0.0 13 0.0 0.0 6.373586497854438E-5 6.373586497854438E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4500 0.0 15.32395 1 AAATCGC 225 0.0 10.958161 17 CCGATAC 70 0.0014908148 10.858504 3 GTATCAA 10445 0.0 10.845474 1 CCGTACA 1215 0.0 10.770842 17 CCCCGTA 1195 0.0 10.712926 15 CGGGTCT 110 5.9059457E-6 10.384943 6 CCCGTAC 1255 0.0 10.276424 16 CGCCCTA 1195 0.0 10.157549 16 CTACGCT 75 0.0026045197 10.154167 4 TGTCCCG 1225 0.0 9.914374 11 AATCGCC 270 0.0 9.834248 18 TCCCGCC 1285 0.0 9.519922 13 GTCCCGC 1285 0.0 9.51972 12 ATCGCCG 270 0.0 9.483025 19 CCCGCCC 1300 0.0 9.483025 14 GTCCTAT 260 0.0 9.145593 1 TACACCG 115 1.0869706E-4 9.105639 5 GCCCCGT 1205 0.0 9.050189 14 GTACTGG 2305 0.0 9.036878 1 >>END_MODULE