FastQCFastQC Report
Wed 25 May 2016
SRR1294627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902615
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC100970.34785874116960047No Hit
CCCATGTACTCTGCGTTGATACCAC99460.34265653557223397No Hit
GAGTACATGGGAAGCAGTGGTATCA46540.16033817781552154No Hit
GTATCAACGCAGAGTACTTTTTTTT42810.1474876964392453No Hit
ATTCCATTCCATTCCATTCCATTCC37180.1280913934503887No Hit
CATGTACTCTGCGTTGATACCACTG36870.12702339097675716No Hit
GCGTTGATACCACTGCTTCCCATGT30580.10535327626984632No Hit
GGTATCAACGCAGAGTACTTTTTTT29700.10232152731244068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCACG500.001501376413.2984921
AGCGTCG550.00305830312.09683617
CGATAAG550.00305869812.09662612
CGCCCTA1951.8189894E-1210.72320416
TGTCCCG1951.8189894E-1210.71414811
GTCTAGG2550.010.0576831
TGTGCGC951.6499744E-49.9969710
GTCCCGC2105.456968E-129.957087512
CCCCGTA1701.6530976E-89.50465715
GTATTAG5000.09.4989221
GGACCGT800.00452874559.4971226
CTCGCTC1901.0586518E-99.4969583
GTCGCCC2106.730261E-119.49663111
TTTAGAC2750.09.3242863
CTAGGAC2750.09.3242863
CCGCCCT2357.2759576E-129.3024315
TGTCGCC2252.7284841E-119.28607510
TAGACAG3800.09.2471985
TCGCTCT1752.6851922E-89.2257754
TAGGACC1451.7144794E-69.1696354
Produced by FastQC (version 0.11.5)