FastQCFastQC Report
Wed 25 May 2016
SRR1294627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902615
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115460.3977792438887004No Hit
CCCATGTACTCTGCGTTGATACCAC109060.37573016056211384No Hit
GTACATGGGAAGCAGTGGTATCAAC106070.36542910444547416No Hit
GGTATCAACGCAGAGTACTTTTTTT87840.3026236686574003No Hit
TATCAACGCAGAGTACTTTTTTTTT71980.24798328403870304No Hit
GAGTACATGGGAAGCAGTGGTATCA50860.17522130906096745No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46380.15978695073235685No Hit
CATGTACTCTGCGTTGATACCACTG40750.14039064774350027No Hit
ATTCCATTCCATTCCATTCCATTCC35350.12178673368669285No Hit
GCGTTGATACCACTGCTTCCCATGT31950.11007315816944375No Hit
GAATGGAATGGAATGGAATGGAATG30780.10604231012380216No Hit
ACGCAGAGTACATGGGAAGCAGTGG29870.10290720608830313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAG653.4370278E-614.5895714
TGGACCG604.0273689E-412.69135
GGACCGT701.07190834E-412.2380416
ATACCGC550.003025201812.1144236
GACCGTG1001.8866667E-611.4221717
CCCCGTA1553.237801E-1011.01277315
CCCGTAC1701.4370016E-1010.59889316
AGCACCG909.334954E-510.5760845
TATACCG1002.349415E-510.4703245
TAACCCG1105.9112317E-610.3837915
CGCCCTA1201.5599144E-610.27348916
CCGTACA1804.0745363E-1010.01006617
TAGACAG4750.09.8190595
GTACTAA3800.09.2617441
CCGAATA1356.6861376E-69.1657819
TATACTG10200.09.1452025
CCTATAC2501.8189894E-129.1222783
ATACCGT1151.0878998E-49.10462956
CGCCTTT1252.8135926E-59.10389214
CTAAGAC3550.09.1008653
Produced by FastQC (version 0.11.5)