##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2902615 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.471588894841375 31.0 31.0 34.0 29.0 34.0 2 31.56717821688374 31.0 31.0 34.0 28.0 34.0 3 31.73411251578318 33.0 31.0 34.0 30.0 34.0 4 35.32855752485259 37.0 35.0 37.0 33.0 37.0 5 35.47582369690779 37.0 35.0 37.0 33.0 37.0 6 35.13399090130796 37.0 35.0 37.0 32.0 37.0 7 34.96987440635427 37.0 35.0 37.0 32.0 37.0 8 35.01796690225883 37.0 35.0 37.0 32.0 37.0 9 36.492856613777576 39.0 37.0 39.0 32.0 39.0 10 36.26001278157799 39.0 37.0 39.0 32.0 39.0 11 36.44670650430732 39.0 37.0 39.0 32.0 39.0 12 36.38661276125149 39.0 37.0 39.0 32.0 39.0 13 36.27588812157313 39.0 37.0 39.0 32.0 39.0 14 37.392454734782255 40.0 37.0 41.0 31.0 41.0 15 37.4546148903661 40.0 37.0 41.0 32.0 41.0 16 37.48935873341797 40.0 37.0 41.0 32.0 41.0 17 37.518603741798344 40.0 37.0 41.0 32.0 41.0 18 37.524960079101085 40.0 37.0 41.0 32.0 41.0 19 37.53555121847024 40.0 37.0 41.0 31.0 41.0 20 37.59339871116217 40.0 37.0 41.0 32.0 41.0 21 37.4413516777113 40.0 37.0 41.0 31.0 41.0 22 37.45110839708332 40.0 37.0 41.0 31.0 41.0 23 37.46199375390811 40.0 37.0 41.0 31.0 41.0 24 37.39955006089337 40.0 37.0 41.0 31.0 41.0 25 36.388551357999596 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5136122903702329 1101 2 0.49747379993609897 1101 3 0.3933298744438751 1101 4 0.36718341959581835 1101 5 0.33807654489006467 1101 6 0.38646438071263844 1101 7 0.4660396759226799 1101 8 0.5105473939832024 1101 9 0.6703078321597147 1101 10 0.7341754421781843 1101 11 0.7197484367932461 1101 12 0.7754723797009433 1101 13 0.67542082428794 1101 14 0.9519748458100779 1101 15 0.8442240288008804 1101 16 0.9143648972953358 1101 17 0.9262171790037215 1101 18 0.9306788668268311 1101 19 1.109219449692901 1101 20 0.9491883755461785 1101 21 1.1904968099500621 1101 22 1.122909944284963 1101 23 1.06044095654876 1101 24 1.1703682754496114 1101 25 1.046605420764628 1102 1 0.32037205448061457 1102 2 0.3528601182143518 1102 3 0.21289209965321731 1102 4 0.16217102085076363 1102 5 0.260820244454365 1102 6 0.31047939595687524 1102 7 0.33608784089767596 1102 8 0.3236767422802629 1102 9 0.48498748920847845 1102 10 0.6363351162455828 1102 11 0.5241703475542323 1102 12 0.5423999323533835 1102 13 0.6202062076521315 1102 14 0.7182788467373982 1102 15 0.7944979489843647 1102 16 0.7751971679106617 1102 17 0.7070329234306669 1102 18 0.6959763082286585 1102 19 0.7727306346381582 1102 20 0.7313327936991527 1102 21 1.0029092615895223 1102 22 0.9307269336687654 1102 23 0.846006275493906 1102 24 0.8955451052233698 1102 25 0.8883811198051603 1103 1 0.20456151395760003 1103 2 0.17835680269884335 1103 3 0.1742492335883341 1103 4 0.13663965731625183 1103 5 0.1141502919430124 1103 6 0.17983913516891903 1103 7 0.18458249766189283 1103 8 0.21506290232373004 1103 9 0.06875186286294621 1103 10 0.26117358072381336 1103 11 0.28375872481086617 1103 12 0.34495041600663967 1103 13 0.41998827957177554 1103 14 0.573019017098936 1103 15 0.4936123631660294 1103 16 0.45079702147410217 1103 17 0.44574949264344355 1103 18 0.4525878741204039 1103 19 0.5697947837171569 1103 20 0.5186623150897844 1103 21 0.7237440350606548 1103 22 0.6847119822621366 1103 23 0.6676725609913277 1103 24 0.7949711991658006 1103 25 0.6919532996993993 1104 1 0.09766334430491952 1104 2 0.0934627627520257 1104 3 0.04947946092901745 1104 4 0.056906401963402686 1104 5 0.05690440707454769 1104 6 0.08816797083382966 1104 7 0.1771167755369376 1104 8 0.1532661292362718 1104 9 -0.08404973009523786 1104 10 0.3572058222677299 1104 11 0.3901223144454775 1104 12 0.39906043869243746 1104 13 -0.03694881763615854 1104 14 0.2908410673963502 1104 15 0.397953506457668 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2206 25 0.26029287687618563 2207 1 0.14398062153403757 2207 2 0.1934509622252527 2207 3 0.22814494714366518 2207 4 0.1106659180100209 2207 5 0.14310090561266264 2207 6 0.23864053101502236 2207 7 0.2362709293523082 2207 8 0.25213130192830135 2207 9 0.31708770291280786 2207 10 0.4684757146912517 2207 11 0.41750058482369923 2207 12 0.3955915330804771 2207 13 0.47289953061955003 2207 14 0.4415278640445166 2207 15 0.46833592139653746 2207 16 0.5059428905432952 2207 17 0.49001145145258107 2207 18 0.40450904807269694 2207 19 0.5695978070118599 2207 20 0.44821279951264614 2207 21 0.7969481270156393 2207 22 0.6599894810898235 2207 23 0.5993289218668494 2207 24 0.6956368691604169 2207 25 0.6253260945339036 2208 1 0.1399305725132045 2208 2 0.10635699345334615 2208 3 0.15838106480676117 2208 4 0.14543442686081676 2208 5 0.10971631338906462 2208 6 0.18626111486266694 2208 7 0.18958678963825548 2208 8 0.14575770728907145 2208 9 0.2366071700161072 2208 10 0.24687219309706876 2208 11 -0.04302649329871144 2208 12 -0.16123081590108512 2208 13 -0.019978388028569327 2208 14 -0.40001838319371785 2208 15 0.12237079716845756 2208 16 0.20939950007715424 2208 17 0.1538703988526251 2208 18 0.23379198415904057 2208 19 0.41096273194917643 2208 20 0.35486916884951114 2208 21 0.682166762408059 2208 22 0.6266886043165982 2208 23 0.5373882977770563 2208 24 0.7531713500438713 2208 25 0.6609679545856153 2209 1 0.04628993061139042 2209 2 0.0345426385071832 2209 3 0.11601504358793235 2209 4 -0.5451364425595671 2209 5 -0.09892158840400356 2209 6 -0.02999547572569128 2209 7 0.015464072166743392 2209 8 0.052709972087448875 2209 9 0.07994313152563848 2209 10 0.19292383966960358 2209 11 0.21069336102780056 2209 12 0.2114604633894217 2209 13 0.19467745520855573 2209 14 0.3201039098295695 2209 15 -0.2971278748638113 2209 16 -0.37026625142900826 2209 17 -0.00916148222307811 2209 18 -0.28919649842962514 2209 19 0.18380738121525297 2209 20 0.2390089090227292 2209 21 0.4447818962256562 2209 22 0.379839169710273 2209 23 0.3768118809796519 2209 24 0.5374142024791624 2209 25 0.48647507618196784 2210 1 -0.1942839071122009 2210 2 -0.1737392606510184 2210 3 -0.07002712609137163 2210 4 -7.364754944916285E-5 2210 5 -0.09065235519465631 2210 6 -0.4078161983962403 2210 7 -0.13870839500601306 2210 8 -0.22579634321355968 2210 9 0.03114580755287477 2210 10 0.10218353195276109 2210 11 0.09107706903757418 2210 12 0.08148992814772527 2210 13 0.12501575613156035 2210 14 0.14905083701146538 2210 15 0.11484434705629809 2210 16 0.14042457978351308 2210 17 -0.9147091904793712 2210 18 -1.4495408400359366 2210 19 -0.28949522303071973 2210 20 -0.12067163716742613 2210 21 -0.3678029588341971 2210 22 -0.33698250188682266 2210 23 -0.21513407099215698 2210 24 0.1761917639464059 2210 25 0.1265202521548261 2211 1 -0.16897168397947837 2211 2 -0.17187118327786166 2211 3 -0.12952765374559405 2211 4 -0.10591839323221564 2211 5 -0.05713669641306751 2211 6 -0.019535050922179664 2211 7 -0.12882072941481226 2211 8 -0.15823619214419438 2211 9 -0.07959569025940283 2211 10 -0.004621967746373912 2211 11 -0.2620561387070026 2211 12 -0.11883035342913217 2211 13 -0.16742941469838257 2211 14 -0.07201209142410647 2211 15 -0.2920799277593247 2211 16 -0.18699638547597175 2211 17 -0.3149668187870347 2211 18 -0.23801545064526408 2211 19 -0.15374333844336263 2211 20 -0.3337321223674081 2211 21 0.10884004150749149 2211 22 -0.019193841765684283 2211 23 -0.5613461184417261 2211 24 -0.025266134084795056 2211 25 -0.7571124581735944 2212 1 -0.19351570245262906 2212 2 -0.3346934337670646 2212 3 -0.18322084524410798 2212 4 -0.029499915447800618 2212 5 -0.06924491521274234 2212 6 -0.19472471998345497 2212 7 -0.1744296007627355 2212 8 -0.2942350210309925 2212 9 -0.0839893166246668 2212 10 -0.5016897517123766 2212 11 -0.1525382635165684 2212 12 -0.2597878211780298 2212 13 -0.2735408722546282 2212 14 -0.34075941382594976 2212 15 -0.21732165180998209 2212 16 -0.3570649816122895 2212 17 -0.6321398375298131 2212 18 -0.4725808237391007 2212 19 -0.49310571031150374 2212 20 -0.6176975646032403 2212 21 -0.13318085657753898 2212 22 -0.4652241153727985 2212 23 -0.37544185529192475 2212 24 -0.5069319599098279 2212 25 -0.3110495323424374 2213 1 -0.16200505313502234 2213 2 -0.2017881904258374 2213 3 -0.14861727001648362 2213 4 -0.03616972208862279 2213 5 -0.10957084116772364 2213 6 -0.1948978061554314 2213 7 -0.2984177891550246 2213 8 -0.263300578451819 2213 9 -0.3092848204609311 2213 10 -0.3146554680418632 2213 11 -0.04233357633462731 2213 12 0.03330002352157635 2213 13 0.04896938017660091 2213 14 -0.6668611868139394 2213 15 -0.1974888204102072 2213 16 -0.3215313921777323 2213 17 -0.23766867637586842 2213 18 -0.30199147758174405 2213 19 -0.28348182495212626 2213 20 -0.4128971866855906 2213 21 -0.14589228823624012 2213 22 -0.4620114090685803 2213 23 -0.49653072772132845 2213 24 -0.6239345697214773 2213 25 -0.4294277254180656 2214 1 -0.2843023395892956 2214 2 -0.1393506010522323 2214 3 -0.17399543485962 2214 4 -0.0595242899139663 2214 5 -0.10745739164249812 2214 6 -0.07078633757799935 2214 7 -0.08336206542018232 2214 8 -0.1183161483082813 2214 9 -0.2975509359743924 2214 10 -0.25326488085464405 2214 11 0.008375477776532136 2214 12 -0.28484406751585567 2214 13 -0.2920067173215415 2214 14 -0.1616947760666818 2214 15 -0.05019395917847902 2214 16 -0.3067929786332684 2214 17 -0.30012918066891103 2214 18 -0.2450541194536484 2214 19 -0.6135515051017748 2214 20 -0.35564516406444113 2214 21 -0.6273997313924227 2214 22 -0.7360528823258576 2214 23 -0.9370967999638964 2214 24 -0.5323470342232355 2214 25 -0.7979359178993448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 13.0 9 47.0 10 101.0 11 180.0 12 397.0 13 944.0 14 1885.0 15 3273.0 16 4712.0 17 6092.0 18 7447.0 19 8392.0 20 9497.0 21 10505.0 22 11828.0 23 13087.0 24 15050.0 25 17224.0 26 20035.0 27 22986.0 28 27332.0 29 34539.0 30 44864.0 31 59243.0 32 81041.0 33 110717.0 34 155537.0 35 220237.0 36 320929.0 37 517237.0 38 1177242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89707788109999 22.179626619290026 12.289637992166577 24.633657507443406 2 14.287771543935381 22.142826382417233 40.497103473936434 23.07229859971095 3 17.47473883341221 27.90815601417909 30.405572289780043 24.21153286262865 4 11.608142996885544 17.89011578166492 39.874264068197576 30.627477153251952 5 12.602819219431979 38.28741344577313 36.01992875106222 13.089838583732663 6 29.66228045541987 40.08120044063109 17.71455526340181 12.541963840547227 7 24.638167586445736 33.686243631470404 24.742407299881286 16.933181482202578 8 23.09093985321244 38.806354339068314 21.688294727425784 16.414411080293466 9 23.88490090993905 16.5722804150193 23.020920467496172 36.521898207545476 10 13.974686923675186 30.720297734175695 34.632381496465584 20.672633845683535 11 31.88378916653078 26.17119075354945 23.559060199768396 18.385959880151372 12 19.923853714517232 28.090613459584624 33.199532414538695 18.786000411359446 13 26.19699822401524 24.21561247357986 27.691960525250508 21.895428777154393 14 19.486994682665294 23.327747502051622 29.68629310041887 27.498964714864215 15 22.690894357904398 30.578755270322134 24.88008918980188 21.85026118197159 16 20.696234257729667 31.695143861655783 27.0883324174925 20.520289463122047 17 19.183019168239387 30.798342459589872 29.893847407888202 20.12479096428254 18 19.506271090968287 30.229845259689142 30.69776976198966 19.566113887352913 19 20.041349291959694 29.22449578634798 31.04338919495664 19.690765726735684 20 21.371546337482133 28.7482253022886 30.043681421563445 19.836546938665826 21 21.41476857544368 29.514550530229872 29.238695256577792 19.83198563774866 22 19.920655154830975 29.282554469908906 30.49501812218348 20.301772253076635 23 19.81728870410513 29.665315240204336 30.256127928913685 20.261268126776848 24 19.99738167135497 28.88901903972797 30.337023683816145 20.776575605100916 25 20.742574120980432 28.649374757110298 29.32016377312103 21.28788734878824 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1215.0 1 1215.0 2 1453.0 3 1691.0 4 1691.0 5 1691.0 6 4005.5 7 6320.0 8 6320.0 9 6320.0 10 9736.0 11 13152.0 12 13152.0 13 13152.0 14 21972.0 15 30792.0 16 30792.0 17 30792.0 18 49448.5 19 68105.0 20 68105.0 21 68105.0 22 97282.0 23 126459.0 24 126459.0 25 126459.0 26 163617.5 27 200776.0 28 200776.0 29 200776.0 30 237534.0 31 274292.0 32 274292.0 33 274292.0 34 302648.5 35 331005.0 36 331005.0 37 331005.0 38 345345.0 39 359685.0 40 359685.0 41 359685.0 42 351100.5 43 342516.0 44 342516.0 45 342516.0 46 345174.0 47 347832.0 48 347832.0 49 347832.0 50 316573.0 51 285314.0 52 285314.0 53 285314.0 54 245866.0 55 206418.0 56 206418.0 57 206418.0 58 174266.5 59 142115.0 60 142115.0 61 142115.0 62 115875.5 63 89636.0 64 89636.0 65 89636.0 66 67290.0 67 44944.0 68 44944.0 69 44944.0 70 32255.0 71 19566.0 72 19566.0 73 19566.0 74 13470.5 75 7375.0 76 7375.0 77 7375.0 78 4939.5 79 2504.0 80 2504.0 81 2504.0 82 1585.5 83 667.0 84 667.0 85 667.0 86 415.5 87 164.0 88 164.0 89 164.0 90 108.5 91 53.0 92 53.0 93 53.0 94 33.0 95 13.0 96 13.0 97 13.0 98 9.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09415647614306409 2 0.0 3 0.12743681128913067 4 0.011782478902644684 5 0.023323795956404828 6 0.01426300077688567 7 0.02239360025356446 8 0.0206021122332793 9 1.0335507809337442E-4 10 0.3757990639475094 11 0.06738751091688013 12 0.001515874478702825 13 0.0 14 0.0011369058590271187 15 0.0014125194006094504 16 0.0 17 3.4451692697791474E-5 18 3.4451692697791474E-4 19 8.957440101425783E-4 20 2.756135415823318E-4 21 2.0671015618674884E-4 22 0.00327291080629019 23 1.0335507809337442E-4 24 0.0 25 0.002170456639960863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2902615.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.78769607391732 #Duplication Level Percentage of deduplicated Percentage of total 1 89.55564364592897 70.55882833279496 2 8.097169747794096 12.75914698296238 3 1.3036566895186938 3.0813632111558418 4 0.3695895212586585 1.1647642749212717 5 0.18440835298739947 0.7264554634331446 6 0.10088005097090838 0.4768864077490331 7 0.0675914391120375 0.3727761633370528 8 0.04480138892197963 0.2823838571259438 9 0.03935120367300532 0.2790351607618406 >10 0.20438372649736464 3.0049126536224695 >50 0.017942746815794604 0.9973968308145356 >100 0.01190555170517629 1.8094688498540483 >500 0.0013599013004465352 0.7706381947613462 >1k 0.0010528268123612583 1.8451837143543106 >5k 1.316033515451573E-4 0.7281609847718848 >10k+ 1.316033515451573E-4 1.1425989175799194 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11546 0.3977792438887004 No Hit CCCATGTACTCTGCGTTGATACCAC 10906 0.37573016056211384 No Hit GTACATGGGAAGCAGTGGTATCAAC 10607 0.36542910444547416 No Hit GGTATCAACGCAGAGTACTTTTTTT 8784 0.3026236686574003 No Hit TATCAACGCAGAGTACTTTTTTTTT 7198 0.24798328403870304 No Hit GAGTACATGGGAAGCAGTGGTATCA 5086 0.17522130906096745 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4638 0.15978695073235685 No Hit CATGTACTCTGCGTTGATACCACTG 4075 0.14039064774350027 No Hit ATTCCATTCCATTCCATTCCATTCC 3535 0.12178673368669285 No Hit GCGTTGATACCACTGCTTCCCATGT 3195 0.11007315816944375 No Hit GAATGGAATGGAATGGAATGGAATG 3078 0.10604231012380216 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2987 0.10290720608830313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.4451692697791474E-5 0.0 8 0.0 0.0 0.0 6.890338539558295E-5 0.0 9 0.0 0.0 0.0 1.0335507809337442E-4 0.0 10 0.0 0.0 0.0 1.378067707911659E-4 0.0 11 0.0 0.0 0.0 1.378067707911659E-4 0.0 12 0.0 0.0 0.0 1.378067707911659E-4 0.0 13 0.0 3.4451692697791474E-5 0.0 1.378067707911659E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACGAG 65 3.4370278E-6 14.58957 14 TGGACCG 60 4.0273689E-4 12.6913 5 GGACCGT 70 1.07190834E-4 12.238041 6 ATACCGC 55 0.0030252018 12.114423 6 GACCGTG 100 1.8866667E-6 11.422171 7 CCCCGTA 155 3.237801E-10 11.012773 15 CCCGTAC 170 1.4370016E-10 10.598893 16 AGCACCG 90 9.334954E-5 10.576084 5 TATACCG 100 2.349415E-5 10.470324 5 TAACCCG 110 5.9112317E-6 10.383791 5 CGCCCTA 120 1.5599144E-6 10.273489 16 CCGTACA 180 4.0745363E-10 10.010066 17 TAGACAG 475 0.0 9.819059 5 GTACTAA 380 0.0 9.261744 1 CCGAATA 135 6.6861376E-6 9.165781 9 TATACTG 1020 0.0 9.145202 5 CCTATAC 250 1.8189894E-12 9.122278 3 ATACCGT 115 1.0878998E-4 9.1046295 6 CGCCTTT 125 2.8135926E-5 9.103892 14 CTAAGAC 355 0.0 9.100865 3 >>END_MODULE