FastQCFastQC Report
Wed 25 May 2016
SRR1294626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114124
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC113940.3658813842994049No Hit
CCCATGTACTCTGCGTTGATACCAC105240.33794415379734394No Hit
GTATCAACGCAGAGTACTTTTTTTT55640.1786698281763989No Hit
GAGTACATGGGAAGCAGTGGTATCA52770.16945375328663853No Hit
CATGTACTCTGCGTTGATACCACTG41020.13172243622925742No Hit
GGTATCAACGCAGAGTACTTTTTTT39430.12661666651681178No Hit
GCGTTGATACCACTGCTTCCCATGT32950.10580824655665606No Hit
TATCAACGCAGAGTACTTTTTTTTT32270.10362464693120761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG550.003076290212.0874129
ACCGTCC907.491253E-611.6077548
CGCCCTA2054.1836756E-119.73624516
TGTCCCG2251.8189894E-129.70783511
AGCGACT1304.216712E-69.5044315
CTAGTAC2650.09.3179093
CTAGAAC3200.09.2003153
CGGAACG1451.6968315E-69.17669114
TTACACC2700.09.145654
AACAGTC3450.09.0843297
GTTTACG1054.5004475E-49.0464561
TCTATAC5200.08.949193
CACCGCT850.0074559218.9385917
TAGGACT4100.08.8024734
GTATTAG4000.08.786371
GGACAGT4000.08.7849596
GTCCCGC2401.0186341E-108.71197312
CTATACT5500.08.6340064
TAGACAG3850.08.6338665
TCTTAGG3850.08.6337272
Produced by FastQC (version 0.11.5)