##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294626_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3114124 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.47774751422872 31.0 30.0 33.0 25.0 34.0 2 29.743492873116164 31.0 30.0 34.0 25.0 34.0 3 29.695295691501045 31.0 30.0 34.0 24.0 34.0 4 33.36894516724446 35.0 33.0 37.0 27.0 37.0 5 33.65578538298411 35.0 33.0 37.0 28.0 37.0 6 33.73290434163829 35.0 35.0 37.0 28.0 37.0 7 33.62064195260047 35.0 33.0 37.0 27.0 37.0 8 33.47019258064226 35.0 33.0 37.0 27.0 37.0 9 34.58086415312942 38.0 34.0 39.0 26.0 39.0 10 34.654950798362556 38.0 34.0 39.0 27.0 39.0 11 34.899707269203155 38.0 34.0 39.0 27.0 39.0 12 34.66522688242343 38.0 34.0 39.0 27.0 39.0 13 34.518978370803474 38.0 34.0 39.0 26.0 39.0 14 35.32471346677268 38.0 34.0 41.0 25.0 41.0 15 35.298154472975384 38.0 33.0 40.0 25.0 41.0 16 35.3922001821379 38.0 34.0 40.0 25.0 41.0 17 35.36096667955419 38.0 34.0 40.0 25.0 41.0 18 35.23824581166325 38.0 33.0 40.0 25.0 41.0 19 35.50888018588855 39.0 34.0 40.0 25.0 41.0 20 35.623439207944195 39.0 34.0 41.0 26.0 41.0 21 35.65146859919515 39.0 34.0 41.0 26.0 41.0 22 35.64351901208815 39.0 34.0 41.0 26.0 41.0 23 35.61468040450541 39.0 34.0 40.0 26.0 41.0 24 35.617890295954815 39.0 34.0 41.0 26.0 41.0 25 34.12831345187282 38.0 32.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.050748139234802 1101 2 1.0911486357193318 1101 3 1.4468650635244948 1101 4 1.2640313927934699 1101 5 0.9441260987825757 1101 6 0.9227362586877206 1101 7 0.9546842334410997 1101 8 1.0771234905233271 1101 9 1.3970912445004728 1101 10 1.3133438274829174 1101 11 1.1381534647014888 1101 12 1.377998762195098 1101 13 1.4259865419969486 1101 14 1.460197231665667 1101 15 1.6393346638343118 1101 16 1.5524934459003603 1101 17 1.5111509642514207 1101 18 1.4328766621792752 1101 19 1.4570299543667602 1101 20 1.283211678107925 1101 21 1.1377993085881215 1101 22 1.0668307307099596 1101 23 1.2351261163960388 1101 24 1.2611824135407588 1101 25 1.11425070955098 1102 1 0.9846190920872147 1102 2 0.9162393222110268 1102 3 1.3042625470163998 1102 4 1.1996130425029392 1102 5 0.9410541983501375 1102 6 0.8896140382115476 1102 7 0.8955842467042174 1102 8 0.9900896234716967 1102 9 1.4344757339980205 1102 10 1.2553704493682716 1102 11 1.0798645725430305 1102 12 1.3056380584248473 1102 13 1.2044810536589736 1102 14 1.5917193873022626 1102 15 1.6470220035249383 1102 16 1.3791555221971237 1102 17 1.3869099621126821 1102 18 1.423024665099767 1102 19 1.1640549675190428 1102 20 0.24606885061913886 1102 21 0.691529845564915 1102 22 0.7262429561320332 1102 23 0.969449548567141 1102 24 1.0677581184221836 1102 25 0.9482724592882334 1103 1 0.7063190305549973 1103 2 0.7120307954721952 1103 3 1.0333394311449524 1103 4 0.770497924204804 1103 5 0.5869354325237595 1103 6 0.4699164629960535 1103 7 0.43324836384955745 1103 8 0.5730191336187502 1103 9 0.7516725753827345 1103 10 0.7458312258967581 1103 11 0.5900217132499677 1103 12 0.8881215032600878 1103 13 0.7188687504452886 1103 14 0.6477583965355578 1103 15 0.8706913189026295 1103 16 0.872790369153762 1103 17 0.893794419374899 1103 18 0.9917737964690332 1103 19 0.7950975377779343 1103 20 -0.12595826969047152 1103 21 0.3271734915707114 1103 22 -0.30359959175150664 1103 23 0.35517147946767125 1103 24 0.4781344568343755 1103 25 0.5220453760017563 1104 1 0.29497394833860824 1104 2 0.29052738514734955 1104 3 0.6108370435605863 1104 4 0.5483198123474367 1104 5 0.2987451278168578 1104 6 0.23493016070014505 1104 7 0.20575825054721975 1104 8 0.4106716163574191 1104 9 0.6943310857329479 1104 10 0.6337071131875476 1104 11 0.32822878788683596 1104 12 0.46606526878867527 1104 13 0.4074913665854467 1104 14 0.6861507215322931 1104 15 0.7138576060295918 1104 16 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1107 14 0.6590619982497756 1107 15 0.7388419736467071 1107 16 0.6627108174780219 1107 17 0.14573891295892594 1107 18 -0.15741905053811678 1107 19 0.31724588631396955 1107 20 0.22449818394810706 1107 21 0.4533127953223399 1107 22 0.45708804276396364 1107 23 0.5881353897460428 1107 24 0.4708667852780053 1107 25 0.1440789586135125 1108 1 0.024478025592344466 1108 2 0.1774793432105355 1108 3 0.6649453075007052 1108 4 -0.03299826435650033 1108 5 0.21924384411985898 1108 6 0.24437389394626052 1108 7 0.205131682419875 1108 8 0.3940598843365919 1108 9 0.5082514359810588 1108 10 0.5679889759295946 1108 11 0.26404282435464665 1108 12 0.2785739911956071 1108 13 0.3390669927803529 1108 14 0.6412834955585325 1108 15 0.12784866097340597 1108 16 0.41919426226680656 1108 17 0.40654465427356 1108 18 0.5700316100392797 1108 19 0.37889977638084105 1108 20 0.32024112963396334 1108 21 0.3596902215170914 1108 22 0.203458944737541 1108 23 0.3283082816070788 1108 24 0.3661530354818936 1108 25 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6 0.3333758171098893 2207 7 0.2428209637043679 2207 8 0.35460339232142246 2207 9 -1.3726233869753628 2207 10 -0.33300558462234875 2207 11 0.08583763146798873 2207 12 0.44120318080124576 2207 13 0.5371335089267149 2207 14 0.8042943802023075 2207 15 0.8011409032938133 2207 16 0.8178002145074075 2207 17 0.7743984064734732 2207 18 0.8376551113874839 2207 19 -0.48766043529615644 2207 20 0.2504286422388802 2207 21 -0.3190639996527338 2207 22 0.21850675177284273 2207 23 -0.668151038379527 2207 24 -1.7972632245172662 2207 25 -0.32187478659980684 2208 1 0.5047791914012407 2208 2 0.23891011818641772 2208 3 0.7731662601033626 2208 4 0.6869004825897349 2208 5 0.5220545684488016 2208 6 0.34934340546434584 2208 7 0.4185682342413628 2208 8 0.5568321831469447 2208 9 -0.029540702230335114 2208 10 0.09634700354215653 2208 11 0.30686157378193 2208 12 0.30145381713469277 2208 13 0.6753931721877535 2208 14 0.9266620675760038 2208 15 0.8202361762278585 2208 16 0.6336882688405439 2208 17 0.5626437928262149 2208 18 0.868775340112137 2208 19 -1.480930803174708 2208 20 -0.10046541084670935 2208 21 -0.1972430014456208 2208 22 0.24194845480580085 2208 23 0.3840131080758127 2208 24 0.6105531683146239 2208 25 0.6457321952372652 2209 1 0.16664047394182901 2209 2 -1.493751985627899 2209 3 -0.3269390288557865 2209 4 0.03165523671868442 2209 5 0.019102706640310885 2209 6 0.05063857874456801 2209 7 0.12126327434742024 2209 8 0.26992894876141804 2209 9 -2.0627303424318626 2209 10 -0.5075253675830638 2209 11 -0.12568457324750426 2209 12 0.2552450135487163 2209 13 0.3959825224228908 2209 14 0.531955343471779 2209 15 0.6676137269546061 2209 16 0.6292016294239673 2209 17 0.3005119940720178 2209 18 0.47528712979620025 2209 19 0.3064048502866754 2209 20 0.26911605313623 2209 21 0.3718178726412944 2209 22 0.21926701989888642 2209 23 0.26874417466984113 2209 24 0.3833677277631935 2209 25 0.36589746218596275 2210 1 0.055610708948844234 2210 2 0.07961516719391426 2210 3 0.008239003849197957 2210 4 0.18263619404079634 2210 5 0.08828469776269543 2210 6 0.025121198217675555 2210 7 -0.008945599425182138 2210 8 0.14462486159936816 2210 9 0.31256605848280117 2210 10 -1.4875430459520445 2210 11 -0.46757366069778783 2210 12 -2.412869549968512 2210 13 -0.8542576728077904 2210 14 -0.6668984448874014 2210 15 -0.7040317064536694 2210 16 -0.1531834131386276 2210 17 -0.016897356247923767 2210 18 0.31648445498210975 2210 19 0.18633413681076405 2210 20 0.2596629296374502 2210 21 0.15254172498848106 2210 22 0.18003353091904728 2210 23 0.04372063553723393 2210 24 0.10006129629569926 2210 25 0.018922961311652386 2211 1 -0.29943024736957824 2211 2 -0.013912340896698794 2211 3 -1.3991563601123396 2211 4 -0.5260892824925492 2211 5 -0.5261421460458564 2211 6 -0.4670638097568087 2211 7 -0.5245740350331758 2211 8 -0.05106427297581462 2211 9 0.13225296330001157 2211 10 0.06977412163594465 2211 11 -0.2662760654298282 2211 12 -0.2449083849199667 2211 13 -0.44604937624998087 2211 14 -0.7410564789841132 2211 15 -0.30990185402028914 2211 16 -0.12260785686046205 2211 17 -0.14716322391797831 2211 18 0.018811764872012304 2211 19 -0.004138293160899309 2211 20 -0.04831155202123938 2211 21 -0.16444624360909899 2211 22 -0.33105332125109754 2211 23 -0.4613396763191915 2211 24 -0.24119542007598938 2211 25 -0.22575581417959256 2212 1 -0.48064112900289757 2212 2 -0.4307710467882444 2212 3 -2.4057318351625057 2212 4 -1.496538592162434 2212 5 -0.7449988215947059 2212 6 -0.5210932934038155 2212 7 -0.4293633858449297 2212 8 -0.45627433727302247 2212 9 -1.0400152819376984 2212 10 -0.2012319831453908 2212 11 -0.47023134952742396 2212 12 -0.18474800386723444 2212 13 -0.4440354770020676 2212 14 -0.13295998496005268 2212 15 0.05512154086564891 2212 16 -1.29093094402117 2212 17 -1.4259925108481397 2212 18 -0.26220988108391907 2212 19 -0.16101489641600608 2212 20 -0.23334245225318995 2212 21 -0.16190814899065487 2212 22 -0.4467767188725702 2212 23 -0.047624752212207966 2212 24 -0.18963456956885238 2212 25 -0.06886539721877938 2213 1 -3.0190156557136802 2213 2 -1.4145190232440115 2213 3 -2.4938811473267997 2213 4 -1.0376095973982444 2213 5 -0.8545820109939299 2213 6 -0.7750953389614992 2213 7 -0.43330142305782005 2213 8 -0.21325056951832266 2213 9 -0.441705463486592 2213 10 -0.14657021136731885 2213 11 -0.48605845700500083 2213 12 -0.14281655488538547 2213 13 -0.5723967053301067 2213 14 -0.2023493205150544 2213 15 -0.32868807325547067 2213 16 -1.7438239631604517 2213 17 -1.0925997976393944 2213 18 -0.3982534654609111 2213 19 -0.17639450992752614 2213 20 -0.22357796232179794 2213 21 0.04628875738556815 2213 22 -0.013582013841535456 2213 23 0.04539258084115971 2213 24 0.06729582954067581 2213 25 0.12539507497540825 2214 1 -0.6378354752949207 2214 2 -0.48640036093412675 2214 3 -6.583680843216488 2214 4 -2.1058184097988644 2214 5 -1.1883277285550022 2214 6 -0.8068385819536985 2214 7 -0.5046863186488508 2214 8 -0.3886655789076414 2214 9 -0.026181713778257176 2214 10 -0.06258710937099465 2214 11 -0.6734002638460694 2214 12 -0.42002762275105 2214 13 -5.285828006926721 2214 14 -3.870185353384052 2214 15 -2.150518916934317 2214 16 -2.8173170907707075 2214 17 -3.394065250447156 2214 18 -0.9540126220047327 2214 19 -0.6278212473661782 2214 20 -0.18042781585952383 2214 21 -0.14904467370785568 2214 22 -0.03292790658026945 2214 23 -0.021364256996584174 2214 24 -0.004071213634105675 2214 25 0.26114557276796546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1209.0 3 159.0 4 166.0 5 302.0 6 45.0 7 50.0 8 81.0 9 289.0 10 931.0 11 2274.0 12 4851.0 13 8442.0 14 12843.0 15 17155.0 16 20989.0 17 23261.0 18 24928.0 19 25913.0 20 26877.0 21 27674.0 22 28887.0 23 30531.0 24 32793.0 25 35815.0 26 39382.0 27 44732.0 28 51872.0 29 62332.0 30 75410.0 31 92592.0 32 117196.0 33 151236.0 34 197303.0 35 259582.0 36 350495.0 37 500486.0 38 845041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79394951025156 20.809929134093647 13.063504813590876 23.332616542063917 2 15.045318831821206 20.994903458727592 40.71331015584563 23.24646755360557 3 18.585438435110664 26.753832011898215 29.28705477671725 25.373674776273862 4 12.11898944432932 17.20922910351497 38.44487388937349 32.22690756278222 5 12.78734113530249 38.53152620305703 35.22628591292324 13.454846748717245 6 30.67174073290973 38.401990466685135 17.553857059402308 13.372411741002828 7 25.81787300002121 33.090158018969476 23.27182617334981 17.820142807659508 8 24.121145210254248 37.50575149924556 21.074939143354353 17.29816414714584 9 24.634650434049423 16.864979396469508 21.75317664539049 36.74719352409058 10 14.674909329742189 30.37597430160525 33.500519424016126 21.448596944636435 11 32.199714935307796 26.13495718246068 22.77352651243013 18.891801369801392 12 20.55480850639972 28.04997678524666 31.547998309901693 19.847216398451923 13 26.635895540971493 24.024676879081685 26.53169356328672 22.807734016660106 14 20.02992125427636 23.44602751328736 28.172056209029776 28.351995023406506 15 23.193181938679736 30.480221150629788 23.971212887043325 22.355384023647154 16 21.257591044334912 31.205955877155212 26.117690908740382 21.41876216976949 17 20.058937050911048 30.365305607020776 28.479686286001826 21.09607105606635 18 20.430032149832755 29.665857829439318 29.300723451627654 20.603386569100273 19 20.931176391766193 29.031532465145393 29.489469630292298 20.547821512796116 20 22.292105602234326 28.379499956461235 28.60387723160278 20.72451720970166 21 22.211554619891157 28.847863603779544 28.08686512905389 20.85371664727541 22 21.196932077107448 28.570023903680923 28.827440744481255 21.40560327473038 23 20.90891213440211 28.997903019638866 28.72095123090755 21.37223361505147 24 21.01438470225061 28.587739870903388 28.730455707315883 21.66741971953012 25 21.58257984782688 28.271178946407787 27.920277173839697 22.225964031925635 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3145.0 1 3145.0 2 3176.5 3 3208.0 4 3208.0 5 3208.0 6 4898.5 7 6589.0 8 6589.0 9 6589.0 10 10269.0 11 13949.0 12 13949.0 13 13949.0 14 22930.0 15 31911.0 16 31911.0 17 31911.0 18 49178.0 19 66445.0 20 66445.0 21 66445.0 22 92327.0 23 118209.0 24 118209.0 25 118209.0 26 151734.5 27 185260.0 28 185260.0 29 185260.0 30 219819.0 31 254378.0 32 254378.0 33 254378.0 34 283226.5 35 312075.0 36 312075.0 37 312075.0 38 330889.0 39 349703.0 40 349703.0 41 349703.0 42 352112.5 43 354522.0 44 354522.0 45 354522.0 46 367920.5 47 381319.0 48 381319.0 49 381319.0 50 357275.0 51 333231.0 52 333231.0 53 333231.0 54 296940.0 55 260649.0 56 260649.0 57 260649.0 58 226021.0 59 191393.0 60 191393.0 61 191393.0 62 159195.5 63 126998.0 64 126998.0 65 126998.0 66 97945.0 67 68892.0 68 68892.0 69 68892.0 70 50916.5 71 32941.0 72 32941.0 73 32941.0 74 23015.0 75 13089.0 76 13089.0 77 13089.0 78 8690.5 79 4292.0 80 4292.0 81 4292.0 82 2846.5 83 1401.0 84 1401.0 85 1401.0 86 887.5 87 374.0 88 374.0 89 374.0 90 242.5 91 111.0 92 111.0 93 111.0 94 63.5 95 16.0 96 16.0 97 16.0 98 20.0 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0975234126836311 2 0.05205316165958709 3 0.0553927846161553 4 0.050704467773280706 5 0.047878632963876844 6 0.06711357672334178 7 0.06313171858281816 8 0.06075544840218308 9 0.06537954172666215 10 0.06496208885709111 11 0.05934253099748115 12 0.060337995532612064 13 0.06098023071656748 14 0.06277848923164267 15 0.06541165348585991 16 0.06114078951255634 17 0.06390240080356467 18 0.13840168214239382 19 0.06823748829526377 20 0.06277848923164267 21 0.06091600719817194 22 0.06586121811462871 23 0.06518687117147551 24 0.07533418707797121 25 0.06878338820162588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3114124.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.08088762718513 #Duplication Level Percentage of deduplicated Percentage of total 1 90.83194881012767 73.64735034432194 2 6.921401657154709 11.22386779972748 3 1.1718418612973898 2.8504193481805533 4 0.3744873424149601 1.214550645126023 5 0.17920038031684551 0.7264862949609495 6 0.11376578338460153 0.5534538419055338 7 0.0750679132145537 0.42606011250295606 8 0.05525891168934233 0.3584353287267289 9 0.03760703440188822 0.27442905572980664 >10 0.21296142903036558 3.0832007592330233 >50 0.01555033383214112 0.8858873805030136 >100 0.00883559687360033 1.4602702666376242 >500 9.567444291759875E-4 0.598254742621934 >1k 9.567444279703601E-4 1.6384855684695572 >5k 7.972870233086334E-5 0.3504068106955431 >10k+ 7.972870233086334E-5 0.7084417006572191 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 11394 0.3658813842994049 No Hit CCCATGTACTCTGCGTTGATACCAC 10524 0.33794415379734394 No Hit GTATCAACGCAGAGTACTTTTTTTT 5564 0.1786698281763989 No Hit GAGTACATGGGAAGCAGTGGTATCA 5277 0.16945375328663853 No Hit CATGTACTCTGCGTTGATACCACTG 4102 0.13172243622925742 No Hit GGTATCAACGCAGAGTACTTTTTTT 3943 0.12661666651681178 No Hit GCGTTGATACCACTGCTTCCCATGT 3295 0.10580824655665606 No Hit TATCAACGCAGAGTACTTTTTTTTT 3227 0.10362464693120761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.211175919777119E-5 0.0 4 0.0 0.0 0.0 6.422351839554238E-5 0.0 5 0.0 3.211175919777119E-5 0.0 9.633527759331356E-5 0.0 6 0.0 3.211175919777119E-5 0.0 1.2844703679108476E-4 0.0 7 0.0 3.211175919777119E-5 0.0 1.6055879598885593E-4 3.211175919777119E-5 8 0.0 3.211175919777119E-5 0.0 1.6055879598885593E-4 3.211175919777119E-5 9 0.0 3.211175919777119E-5 0.0 2.2478231438439832E-4 3.211175919777119E-5 10 0.0 3.211175919777119E-5 0.0 2.8900583277994066E-4 3.211175919777119E-5 11 0.0 3.211175919777119E-5 0.0 2.8900583277994066E-4 3.211175919777119E-5 12 0.0 3.211175919777119E-5 0.0 3.2111759197771185E-4 3.211175919777119E-5 13 0.0 3.211175919777119E-5 0.0 3.5322935117548305E-4 3.211175919777119E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCG 55 0.0030762902 12.087412 9 ACCGTCC 90 7.491253E-6 11.607754 8 CGCCCTA 205 4.1836756E-11 9.736245 16 TGTCCCG 225 1.8189894E-12 9.707835 11 AGCGACT 130 4.216712E-6 9.50443 15 CTAGTAC 265 0.0 9.317909 3 CTAGAAC 320 0.0 9.200315 3 CGGAACG 145 1.6968315E-6 9.176691 14 TTACACC 270 0.0 9.14565 4 AACAGTC 345 0.0 9.084329 7 GTTTACG 105 4.5004475E-4 9.046456 1 TCTATAC 520 0.0 8.94919 3 CACCGCT 85 0.007455921 8.938591 7 TAGGACT 410 0.0 8.802473 4 GTATTAG 400 0.0 8.78637 1 GGACAGT 400 0.0 8.784959 6 GTCCCGC 240 1.0186341E-10 8.711973 12 CTATACT 550 0.0 8.634006 4 TAGACAG 385 0.0 8.633866 5 TCTTAGG 385 0.0 8.633727 2 >>END_MODULE