FastQCFastQC Report
Wed 25 May 2016
SRR1294626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114124
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144240.46318001466865166No Hit
CCCATGTACTCTGCGTTGATACCAC122760.3942039559118391No Hit
GTACATGGGAAGCAGTGGTATCAAC117820.3783407468681401No Hit
GGTATCAACGCAGAGTACTTTTTTT115900.37217528910216807No Hit
TATCAACGCAGAGTACTTTTTTTTT93460.3001165014623695No Hit
GAGTACATGGGAAGCAGTGGTATCA57970.18615186806947956No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56320.18085342780184732No Hit
CATGTACTCTGCGTTGATACCACTG47840.15362265600213737No Hit
GCGTTGATACCACTGCTTCCCATGT36270.1164693506103161No Hit
ACGCAGAGTACATGGGAAGCAGTGG34030.10927631655001535No Hit
GTATCAACGCAGAGTACATGGGAAG33100.10628992294462263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA853.862715E-612.3178496
ACCGTCC951.0169097E-612.0231648
AATCGCC700.001512916410.83915118
CCGGGCG803.7101857E-410.7069239
TAGGACT2500.010.2798054
GCTACAC2350.010.10716153
GTAGGAC1554.0630766E-89.8072073
TTAGACT3350.09.6604034
TTAGGAC1804.1818566E-99.5007313
GTATTAC3900.09.2657671
TAAGACA5400.09.1658074
TCCGTAC1151.1103068E-49.0876573
AGACTGT5450.09.0817176
CTAGTAC2106.675691E-109.0483163
GGTGCGC850.0073215128.9584368
AGACAGT5050.08.8586516
CCTATAC3250.08.7699073
GTCTTAG2502.5465852E-118.7488351
CTGCGCA1201.7302764E-48.7241599
TAGACAG5150.08.6866385
Produced by FastQC (version 0.11.5)