##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3114124 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.495071808315917 31.0 31.0 34.0 30.0 34.0 2 31.587532159926837 31.0 31.0 34.0 29.0 34.0 3 31.74716677948598 33.0 31.0 34.0 30.0 34.0 4 35.349483193347474 37.0 35.0 37.0 33.0 37.0 5 35.503962912202596 37.0 35.0 37.0 33.0 37.0 6 35.16599339011549 37.0 35.0 37.0 32.0 37.0 7 35.011768959745986 37.0 35.0 37.0 32.0 37.0 8 35.062549853506155 37.0 35.0 37.0 32.0 37.0 9 36.5404482930031 39.0 37.0 39.0 32.0 39.0 10 36.31470487366592 39.0 37.0 39.0 32.0 39.0 11 36.48874868181228 39.0 37.0 39.0 32.0 39.0 12 36.429870486852806 39.0 37.0 39.0 32.0 39.0 13 36.312368100949094 39.0 37.0 39.0 32.0 39.0 14 37.446680671675246 40.0 37.0 41.0 32.0 41.0 15 37.50087857773165 40.0 37.0 41.0 32.0 41.0 16 37.53079453483548 40.0 37.0 41.0 32.0 41.0 17 37.57343413428624 40.0 37.0 41.0 32.0 41.0 18 37.573213205382956 40.0 37.0 41.0 32.0 41.0 19 37.57849687424136 40.0 37.0 41.0 32.0 41.0 20 37.64171657904438 40.0 37.0 41.0 32.0 41.0 21 37.48692858730096 40.0 37.0 41.0 31.0 41.0 22 37.503610003969015 40.0 37.0 41.0 31.0 41.0 23 37.51447565992876 40.0 37.0 41.0 31.0 41.0 24 37.44973289438699 40.0 37.0 41.0 31.0 41.0 25 36.42977350934002 39.0 35.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5228911852087244 1101 2 0.49617225122025843 1101 3 0.3730371771837824 1101 4 0.3421523788765697 1101 5 0.3645546684418761 1101 6 0.47456294794471177 1101 7 0.4985933654547381 1101 8 0.4980816222283835 1101 9 0.7007187722575665 1101 10 0.6314303275122626 1101 11 0.674086981721544 1101 12 0.76039522366532 1101 13 0.7769736396050675 1101 14 0.9930908930797386 1101 15 0.964865310679734 1101 16 1.0106310855625793 1101 17 0.9672496612605457 1101 18 0.9762124406801007 1101 19 1.114894342158479 1101 20 0.9511094041222989 1101 21 1.213976907387071 1101 22 1.1400755775533185 1101 23 1.1422258540414418 1101 24 1.2390219244232341 1101 25 1.1256195666819195 1102 1 0.35556075425341405 1102 2 0.36546781389687766 1102 3 0.32304198330314904 1102 4 0.29413139697099666 1102 5 0.32501741298482045 1102 6 0.40207808826384195 1102 7 0.422258107882989 1102 8 0.4375904164880353 1102 9 0.5271161077846216 1102 10 0.6719711570218649 1102 11 0.6083291244987024 1102 12 0.6030279068521338 1102 13 0.5880405496009757 1102 14 0.7937834436677278 1102 15 0.7855868617518809 1102 16 0.8446736974566278 1102 17 0.6713973918255789 1102 18 0.7097110092492898 1102 19 0.8040209808222158 1102 20 0.8047627563344903 1102 21 1.024924538001514 1102 22 0.9856277891948864 1102 23 0.9400039091700023 1102 24 1.0088874568700703 1102 25 0.9129353284116775 1103 1 0.3006151384670268 1103 2 0.2760298446560654 1103 3 0.21081719868032422 1103 4 0.18359427897389224 1103 5 0.18432287747231157 1103 6 0.1862231335941118 1103 7 0.2392162019644175 1103 8 0.27475120356984206 1103 9 0.21372682453978342 1103 10 0.4015451430234336 1103 11 0.43373477865765864 1103 12 0.463491641771121 1103 13 0.5774188896843953 1103 14 0.6860109502404867 1103 15 0.6215268081548402 1103 16 0.6619544464080747 1103 17 0.5892397736240369 1103 18 0.5728713199898579 1103 19 0.628678387092279 1103 20 0.604272978352185 1103 21 0.7823875537951608 1103 22 0.6671455184888941 1103 23 0.7773829712621776 1103 24 0.8991594269563024 1103 25 0.7717829055590641 1104 1 0.17756824386261982 1104 2 0.13711510031392038 1104 3 0.14783450006861898 1104 4 0.14576141707172496 1104 5 0.14240105587986562 1104 6 0.16078297463451463 1104 7 0.22156495164633583 1104 8 0.22452374188283386 1104 9 -0.08364464470235333 1104 10 0.3843347504669197 1104 11 0.4216326087254103 1104 12 0.43684019094099824 1104 13 -0.06280608529506537 1104 14 0.26402500431382236 1104 15 0.44390033165083054 1104 16 0.4320079750339687 1104 17 0.4134964663356655 1104 18 0.41304720890614277 1104 19 0.5176194567074148 1104 20 0.49760065382649543 1104 21 0.6637331926820735 1104 22 0.5888264284652607 1104 23 0.5101174451404376 1104 24 0.6442448732468051 1104 25 0.6771185411320317 1105 1 0.042696493775331135 1105 2 -0.017704523666367322 1105 3 -0.04739695528225596 1105 4 -0.11022850122551375 1105 5 0.0022717411990171854 1105 6 -0.035262189150998324 1105 7 -0.07117380763802572 1105 8 -0.12293666122952374 1105 9 0.1215775583101788 1105 10 0.1355825432549267 1105 11 0.1330614029669306 1105 12 -0.2583713386101181 1105 13 -0.4406996027879089 1105 14 -0.8138268408662555 1105 15 -0.22596174045940387 1105 16 -0.06562968981913286 1105 17 0.08869749805168681 1105 18 -0.70145258611592 1105 19 -0.6750914118281202 1105 20 -0.5715244429760986 1105 21 -0.016131013261109217 1105 22 0.13518594993885102 1105 23 0.1110204061196498 1105 24 0.27588066808239375 1105 25 0.05625747481816745 1106 1 -0.3371325064223534 1106 2 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2207 3 0.23776322797058214 2207 4 0.1622781143840939 2207 5 0.06332272608342038 2207 6 0.1915891273352699 2207 7 0.1812098426159281 2207 8 0.18223053797139954 2207 9 0.2853965830675236 2207 10 0.4099106924959415 2207 11 0.33257781477514214 2207 12 0.3083442482550538 2207 13 0.3741855123556519 2207 14 0.43720913682241047 2207 15 0.46848231162314846 2207 16 0.5257270346644347 2207 17 0.5036593177899391 2207 18 0.4122504504288358 2207 19 0.54096049273533 2207 20 0.49016392733971514 2207 21 0.763690581940935 2207 22 0.6828425792983737 2207 23 0.5808861329804103 2207 24 0.7514193788087979 2207 25 0.8105145280837505 2208 1 0.15589414682278502 2208 2 0.19871173625412908 2208 3 0.18865789379901798 2208 4 0.17362929814199646 2208 5 0.1589910018128009 2208 6 0.21511710456083932 2208 7 0.22239657844900762 2208 8 0.11993787483168461 2208 9 0.3182877611636812 2208 10 0.371518431683171 2208 11 -0.003397788255732337 2208 12 -0.17373571958820122 2208 13 0.10388381003348002 2208 14 -0.40552906389498844 2208 15 0.18998627668209878 2208 16 0.2798790414585284 2208 17 0.25419565160243707 2208 18 0.3545544615660532 2208 19 0.4611435067683658 2208 20 0.429734492644009 2208 21 0.6256317757845835 2208 22 0.6239782123781552 2208 23 0.6410144658419341 2208 24 0.6966437223467494 2208 25 0.6351822070015984 2209 1 -0.005297841292762229 2209 2 -0.018559175909473424 2209 3 0.06903455239783085 2209 4 -0.6192730252608314 2209 5 -0.1500146774399127 2209 6 0.034567230242714686 2209 7 0.11234143323260781 2209 8 0.12724826291229618 2209 9 0.18741160023134285 2209 10 0.2273217288848315 2209 11 0.21032277226656504 2209 12 0.29213646695883 2209 13 0.3010576300975245 2209 14 0.2736224817489159 2209 15 -0.33641927138116756 2209 16 -0.28062717912432333 2209 17 0.06962658710624936 2209 18 -0.3856202724577642 2209 19 0.20178330837657654 2209 20 0.19390821248011036 2209 21 0.4962547668841424 2209 22 0.4775805138499152 2209 23 0.4375144266438724 2209 24 0.5688342884163191 2209 25 0.5477929663225538 2210 1 -0.13653571143361276 2210 2 -0.08981549304404268 2210 3 -0.024435980451269046 2210 4 0.0029027592167878424 2210 5 0.024667784465123077 2210 6 -0.39149010916275273 2210 7 -0.07336983288449517 2210 8 -0.2856876914313915 2210 9 0.014261583898068864 2210 10 0.2334370994580297 2210 11 0.15097245232652057 2210 12 0.16724263325079392 2210 13 0.1515764517958047 2210 14 0.16930377150122666 2210 15 0.11291010353475883 2210 16 0.2429965022038587 2210 17 -0.8978279199170558 2210 18 -1.4224258680458561 2210 19 -0.25398329872167125 2210 20 -0.06353399654739178 2210 21 -0.3360697464803195 2210 22 -0.31376092847440873 2210 23 -0.16330223714044934 2210 24 0.1814637536456516 2210 25 0.283336095778175 2211 1 -0.24714874003335296 2211 2 -0.21723687084865873 2211 3 -0.09153311428320166 2211 4 -0.07763021487775745 2211 5 -0.07405165314036566 2211 6 -0.10099135565940287 2211 7 -0.11857507900951703 2211 8 -0.07531467150293025 2211 9 0.036758912833825264 2211 10 0.011336171940648399 2211 11 -0.3086611860500952 2211 12 -0.07440583921015076 2211 13 -0.07176477859703567 2211 14 -0.01941117561944594 2211 15 -0.13110593833513917 2211 16 -0.09652131263332109 2211 17 -0.20738026503403972 2211 18 -0.3484660346583297 2211 19 -0.12874832249027435 2211 20 -0.30411094642428793 2211 21 0.016650093720357972 2211 22 -0.0857263672257389 2211 23 -0.76771220291986 2211 24 -0.15035365395100797 2211 25 -0.744689174812251 2212 1 -0.2980160639240985 2212 2 -0.4133847210264747 2212 3 -0.27076156289976794 2212 4 -0.1529143668847439 2212 5 -0.12976374891378128 2212 6 -0.22714488698817803 2212 7 -0.36368568462565065 2212 8 -0.5193634958492694 2212 9 -0.29422971776093476 2212 10 -0.6099682801683386 2212 11 -0.3714833200802161 2212 12 -0.535551194910802 2212 13 -0.498541785638551 2212 14 -0.5158404838293649 2212 15 -0.37296409636363137 2212 16 -0.6538112993440564 2212 17 -0.7451562916651753 2212 18 -0.5817858716686928 2212 19 -0.5691404745522703 2212 20 -0.7068878639940976 2212 21 -0.23198542500469443 2212 22 -0.568888442346605 2212 23 -0.5066040263395877 2212 24 -0.6169095087027614 2212 25 -0.3857534830531506 2213 1 -0.2560216226029297 2213 2 -0.17833050020107422 2213 3 -0.16269244743413225 2213 4 -0.05264659299788832 2213 5 -0.14237734397012503 2213 6 -0.3481256257822025 2213 7 -0.3509167536214477 2213 8 -0.32132258017998083 2213 9 -0.3782601437381601 2213 10 -0.356998044613114 2213 11 -0.1585285729639807 2213 12 -0.014536589267684974 2213 13 0.02577461201244091 2213 14 -0.6757410056046211 2213 15 -0.30950123362145376 2213 16 -0.3736789476977833 2213 17 -0.19411217670754155 2213 18 -0.2524127018896607 2213 19 -0.40680515210600277 2213 20 -0.4572286773855012 2213 21 -0.09589262080700678 2213 22 -0.46995502231413866 2213 23 -0.5226871671963309 2213 24 -0.6777147269068706 2213 25 -0.3316218403529234 2214 1 -0.2993715053237551 2214 2 -0.09079127593928504 2214 3 -0.15959324162291466 2214 4 -0.03623706041366859 2214 5 -0.04492969930988977 2214 6 -0.16031563767810297 2214 7 -0.1481439374100333 2214 8 -0.075998826281932 2214 9 -0.1766694736875749 2214 10 0.03419905409915458 2214 11 0.07297352406910562 2214 12 -0.07633526434172211 2214 13 -0.11988588720435445 2214 14 -0.1973617968316148 2214 15 -0.0029117420938433725 2214 16 -0.21719276961815837 2214 17 -0.3394671426897915 2214 18 -0.2517481440479017 2214 19 -0.616289291057619 2214 20 -0.43861736875595625 2214 21 -0.5839087746745477 2214 22 -0.7079060576818179 2214 23 -0.8959453543737155 2214 24 -0.5679261219131249 2214 25 -1.1637931197282185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 22.0 9 49.0 10 94.0 11 197.0 12 404.0 13 935.0 14 1842.0 15 3234.0 16 4783.0 17 6303.0 18 7770.0 19 8897.0 20 9969.0 21 11294.0 22 11956.0 23 13704.0 24 15466.0 25 17801.0 26 20755.0 27 23738.0 28 28586.0 29 35943.0 30 46945.0 31 62160.0 32 85639.0 33 118005.0 34 165213.0 35 236749.0 36 346860.0 37 562014.0 38 1266793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80223720722276 21.118629325665335 12.161346862905246 24.917786604206665 2 15.130001245936256 21.200825657552492 39.444511522341436 24.224661574169815 3 18.227420017607027 26.40970354217616 29.768028392455342 25.59484804776147 4 12.162467261986007 17.166329183440464 38.293046775014574 32.37815677955895 5 12.941418307140356 37.9522263227373 35.4233112676373 13.68304410248504 6 30.96217901592021 38.55865369514088 17.112502645660683 13.366664643278222 7 26.03237191470445 33.08719705934123 23.04828677847029 17.832144247484035 8 24.34435107040397 37.59769050209995 20.612294453273652 17.445663974222427 9 24.533110177584796 16.061401655300152 21.94107609281344 37.46441207430162 10 14.545942128624617 30.074865406973277 33.79022279122553 21.588969673176575 11 32.74143685571772 25.2167114865362 22.739692045884322 19.30215961186175 12 20.761032778820354 27.483041562141548 31.802224988150606 19.95370067088749 13 27.15994198045485 23.139452102566278 26.821419706286488 22.879186210692385 14 20.228863006528393 22.756631953475974 28.534178524063208 28.480326515932425 15 23.48826477872969 29.82215975340445 24.23500049934379 22.454574968522074 16 21.300367358997086 30.711147932642284 26.43298909483257 21.55549561352806 17 20.07853256873045 29.945583377915185 28.77186571367467 21.204018339679692 18 20.40365843555061 29.167905647762865 29.76858937265704 20.65984654402948 19 20.847526858516886 28.478152247072664 30.272798269545966 20.401522624864487 20 22.11056228440926 27.758871051129084 29.447278491052682 20.68328817340897 21 22.10386510971332 28.38860209446276 28.728602274289077 20.778930521534843 22 21.0001541415919 28.073422778273745 29.56689423960026 21.3595288405341 23 20.74918709029383 28.45505089395984 29.484843561042247 21.310918454704087 24 20.85984428355729 28.208946213411036 29.46962257740705 21.461586925624623 25 21.355350807560004 27.812105821573812 28.67952314525804 22.15302022560814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1337.0 1 1337.0 2 1522.5 3 1708.0 4 1708.0 5 1708.0 6 3814.0 7 5920.0 8 5920.0 9 5920.0 10 8974.0 11 12028.0 12 12028.0 13 12028.0 14 19341.5 15 26655.0 16 26655.0 17 26655.0 18 43325.0 19 59995.0 20 59995.0 21 59995.0 22 87240.0 23 114485.0 24 114485.0 25 114485.0 26 149903.5 27 185322.0 28 185322.0 29 185322.0 30 221238.5 31 257155.0 32 257155.0 33 257155.0 34 286358.5 35 315562.0 36 315562.0 37 315562.0 38 332892.5 39 350223.0 40 350223.0 41 350223.0 42 351823.0 43 353423.0 44 353423.0 45 353423.0 46 369555.5 47 385688.0 48 385688.0 49 385688.0 50 362228.5 51 338769.0 52 338769.0 53 338769.0 54 301788.0 55 264807.0 56 264807.0 57 264807.0 58 229088.5 59 193370.0 60 193370.0 61 193370.0 62 161035.0 63 128700.0 64 128700.0 65 128700.0 66 98784.5 67 68869.0 68 68869.0 69 68869.0 70 50485.0 71 32101.0 72 32101.0 73 32101.0 74 22221.5 75 12342.0 76 12342.0 77 12342.0 78 8203.0 79 4064.0 80 4064.0 81 4064.0 82 2636.5 83 1209.0 84 1209.0 85 1209.0 86 760.0 87 311.0 88 311.0 89 311.0 90 187.5 91 64.0 92 64.0 93 64.0 94 38.5 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09675273046288459 2 0.0 3 0.1285112603094803 4 0.01249147432793299 5 0.022285560883253206 6 0.017179791170807587 7 0.025946301431799122 8 0.023120466622395253 9 1.9267055518662712E-4 10 0.3732349771556945 11 0.06541165348585991 12 0.0012844703679108474 13 3.211175919777119E-5 14 0.0010917998127242202 15 0.001188135090317534 16 1.2844703679108476E-4 17 6.422351839554238E-5 18 1.9267055518662712E-4 19 8.991292575375933E-4 20 1.9267055518662712E-4 21 2.568940735821695E-4 22 0.003339622956568203 23 6.422351839554238E-5 24 6.422351839554238E-5 25 0.0016698114782841016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3114124.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.7858047161619 #Duplication Level Percentage of deduplicated Percentage of total 1 89.34926659257629 69.50104602702427 2 8.011629751942333 12.463821346855578 3 1.3597303935575011 3.173031685796813 4 0.4602197061778514 1.4319424076511904 5 0.22498837424394189 0.8750450871173007 6 0.12388920305251741 0.5782092813050428 7 0.08971614751767404 0.4885049911487316 8 0.05606497990745382 0.34888476627973947 9 0.048282800521479395 0.33801448432618764 >10 0.24533529704124155 3.4239472569536544 >50 0.017489716057747108 0.9466816142702946 >100 0.010736093061213002 1.5836483959678338 >500 0.0011597881521577158 0.629024468456119 >1k 0.0012012091535006405 1.9355317726739836 >5k 1.242630158793766E-4 0.66936348404944 >10k+ 1.6568402117250213E-4 1.6133029301238777 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 14424 0.46318001466865166 No Hit CCCATGTACTCTGCGTTGATACCAC 12276 0.3942039559118391 No Hit GTACATGGGAAGCAGTGGTATCAAC 11782 0.3783407468681401 No Hit GGTATCAACGCAGAGTACTTTTTTT 11590 0.37217528910216807 No Hit TATCAACGCAGAGTACTTTTTTTTT 9346 0.3001165014623695 No Hit GAGTACATGGGAAGCAGTGGTATCA 5797 0.18615186806947956 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5632 0.18085342780184732 No Hit CATGTACTCTGCGTTGATACCACTG 4784 0.15362265600213737 No Hit GCGTTGATACCACTGCTTCCCATGT 3627 0.1164693506103161 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3403 0.10927631655001535 No Hit GTATCAACGCAGAGTACATGGGAAG 3310 0.10628992294462263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 3.211175919777119E-5 0.0 0.0 4 0.0 0.0 3.211175919777119E-5 0.0 0.0 5 0.0 0.0 3.211175919777119E-5 0.0 0.0 6 0.0 0.0 3.211175919777119E-5 0.0 3.211175919777119E-5 7 0.0 0.0 3.211175919777119E-5 0.0 3.211175919777119E-5 8 0.0 0.0 3.211175919777119E-5 0.0 3.211175919777119E-5 9 0.0 0.0 3.211175919777119E-5 3.211175919777119E-5 3.211175919777119E-5 10 0.0 0.0 3.211175919777119E-5 6.422351839554238E-5 3.211175919777119E-5 11 0.0 3.211175919777119E-5 3.211175919777119E-5 9.633527759331356E-5 3.211175919777119E-5 12 0.0 3.211175919777119E-5 3.211175919777119E-5 9.633527759331356E-5 3.211175919777119E-5 13 0.0 3.211175919777119E-5 3.211175919777119E-5 1.9267055518662712E-4 3.211175919777119E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGA 85 3.862715E-6 12.317849 6 ACCGTCC 95 1.0169097E-6 12.023164 8 AATCGCC 70 0.0015129164 10.839151 18 CCGGGCG 80 3.7101857E-4 10.706923 9 TAGGACT 250 0.0 10.279805 4 GCTACAC 235 0.0 10.1071615 3 GTAGGAC 155 4.0630766E-8 9.807207 3 TTAGACT 335 0.0 9.660403 4 TTAGGAC 180 4.1818566E-9 9.500731 3 GTATTAC 390 0.0 9.265767 1 TAAGACA 540 0.0 9.165807 4 TCCGTAC 115 1.1103068E-4 9.087657 3 AGACTGT 545 0.0 9.081717 6 CTAGTAC 210 6.675691E-10 9.048316 3 GGTGCGC 85 0.007321512 8.958436 8 AGACAGT 505 0.0 8.858651 6 CCTATAC 325 0.0 8.769907 3 GTCTTAG 250 2.5465852E-11 8.748835 1 CTGCGCA 120 1.7302764E-4 8.724159 9 TAGACAG 515 0.0 8.686638 5 >>END_MODULE