FastQCFastQC Report
Wed 25 May 2016
SRR1294625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4972210
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT134960.27142860015968756No Hit
GTACTGGTTCACTATCGGTCAGTCA86120.17320266038642776No Hit
GGTATCAACGCAGAGTACTTTTTTT85880.1727199776356992No Hit
TATCAACGCAGAGTACTTTTTTTTT80580.16206073355711043No Hit
GGATACCACGTGTCCCGCCCTACTC60220.12111314687030515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.012.5367971
TGTCCCG14200.010.96761211
CGCCCTA15350.010.52687316
CAACGCG1002.410425E-510.44640354
GTCTTAC4550.010.4389071
TCTACGC750.002656234210.1301523
GTATCAA81050.010.0678471
CCGTACA12300.09.96891317
CCCCGTA12400.09.96467215
GTCCCGC15900.09.92291412
TCCCGCC15750.09.83647913
CCCGCCC16050.09.83027414
CCCGTAC12600.09.73146116
CCGCCCT16950.09.64495315
GTACTGG26350.09.6255841
TACTGGT22400.09.6242082
TTACGCA900.00111793479.496734
CACGTGT16550.09.238517
GTCCTAA4450.09.1792011
GCGATAA4500.09.07428711
Produced by FastQC (version 0.11.5)