##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294625_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4972210 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.61888898497851 31.0 30.0 33.0 25.0 34.0 2 29.801063510994105 31.0 30.0 34.0 25.0 34.0 3 29.6658461730297 31.0 30.0 34.0 23.0 34.0 4 33.193860677646356 35.0 33.0 37.0 26.0 37.0 5 33.64508297115367 35.0 33.0 37.0 28.0 37.0 6 33.75318258882871 36.0 35.0 37.0 28.0 37.0 7 33.61296345085988 36.0 35.0 37.0 27.0 37.0 8 33.41611275469057 35.0 33.0 37.0 26.0 37.0 9 34.43810639534533 38.0 34.0 39.0 25.0 39.0 10 34.55440498289493 37.0 34.0 39.0 26.0 39.0 11 34.81337252449112 38.0 34.0 39.0 27.0 39.0 12 34.560279030853486 38.0 34.0 39.0 26.0 39.0 13 34.42312352052709 38.0 34.0 39.0 25.0 39.0 14 35.20421040141104 38.0 33.0 41.0 25.0 41.0 15 35.20198302163424 38.0 33.0 41.0 25.0 41.0 16 35.31213585106019 38.0 34.0 41.0 25.0 41.0 17 35.28301137723467 38.0 34.0 40.0 25.0 41.0 18 35.16355564225968 38.0 33.0 40.0 25.0 41.0 19 35.43640715094495 39.0 34.0 41.0 25.0 41.0 20 35.56091597096663 39.0 34.0 41.0 25.0 41.0 21 35.599935240064276 39.0 34.0 41.0 25.0 41.0 22 35.59335205069778 39.0 34.0 41.0 26.0 41.0 23 35.55880946299533 39.0 34.0 40.0 25.0 41.0 24 35.560961021356704 39.0 34.0 41.0 25.0 41.0 25 34.152150854449026 38.0 32.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2002903113922123 1101 2 1.1764020218696167 1101 3 1.4656053258454484 1101 4 1.3903266397058118 1101 5 1.0192036033537377 1101 6 1.0317717043015548 1101 7 0.9944228772587067 1101 8 1.257801482502984 1101 9 1.5351861425909448 1101 10 1.4603124812963486 1101 11 1.2607627909502597 1101 12 1.512176893542481 1101 13 1.4558067716566754 1101 14 1.532703799150724 1101 15 1.663938479360155 1101 16 1.6263925794610756 1101 17 1.6387141051578524 1101 18 1.6075473714768833 1101 19 1.4690708815893245 1101 20 1.4650281530149272 1101 21 1.3657188184218683 1101 22 1.3108046355192684 1101 23 1.3695598400856426 1101 24 1.4270968775074735 1101 25 1.3903108677492853 1102 1 1.0064411850288266 1102 2 0.9390388982305851 1102 3 1.2417105425767225 1102 4 1.1145225842436375 1102 5 0.9709605035341156 1102 6 0.7943181456757031 1102 7 0.6770628058004817 1102 8 0.7977404717060281 1102 9 1.1563687514324599 1102 10 1.0903293211660667 1102 11 0.8122632824170068 1102 12 1.098009724104692 1102 13 1.113492523934461 1102 14 1.2937245603616958 1102 15 1.4280144297981394 1102 16 1.3180666167614916 1102 17 1.299472048804823 1102 18 1.3403251586749718 1102 19 1.0652765686736956 1102 20 0.21954944653425912 1102 21 0.6111720444803481 1102 22 0.7861911402489596 1102 23 0.8389794589714583 1102 24 0.9618945647785466 1102 25 1.0558831981440306 1103 1 0.6978923244385236 1103 2 0.7193617287704832 1103 3 1.0863438383325672 1103 4 0.8499513171611852 1103 5 0.6475243190577018 1103 6 0.49839217130679714 1103 7 0.46446091519798216 1103 8 0.5539851753748266 1103 9 0.6593828642510431 1103 10 0.6849144832532801 1103 11 0.6127934981254413 1103 12 0.7932710789246755 1103 13 0.7289505910003768 1103 14 0.562086432425545 1103 15 0.8830304632039798 1103 16 0.8423058589651333 1103 17 0.8901284573425698 1103 18 0.9281623126856076 1103 19 0.853701739205853 1103 20 -0.24073843590895194 1103 21 0.2794612356025752 1103 22 -0.3571474788077609 1103 23 0.23123389183762555 1103 24 0.36256749546745226 1103 25 0.4900117899338383 1104 1 0.34253438287440474 1104 2 0.3562485668974027 1104 3 0.6499313626933194 1104 4 0.48389014630598126 1104 5 0.38519120551989516 1104 6 0.22771964808168121 1104 7 0.27328837538050266 1104 8 0.43557569468854496 1104 9 0.686075414046428 1104 10 0.7284396866589518 1104 11 0.37164849852124604 1104 12 0.5428310806163097 1104 13 0.4337539027590296 1104 14 0.7561824795135408 1104 15 0.7990860630089429 1104 16 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-0.17379958710395726 2207 11 0.2484357801966368 2207 12 0.5584937073873419 2207 13 0.721453401543549 2207 14 0.9492567501750884 2207 15 0.9445461863583944 2207 16 0.933407522088828 2207 17 0.9211218732780253 2207 18 0.8709431584715333 2207 19 -0.3455277219541415 2207 20 0.3493059332964563 2207 21 -0.3303225286328413 2207 22 0.3127136132354025 2207 23 -0.47715995014957713 2207 24 -1.6955497146874023 2207 25 -0.25983394844045193 2208 1 0.4832367943035152 2208 2 0.13414508810170744 2208 3 0.6241944612635955 2208 4 0.5868802935719515 2208 5 0.42034080702912746 2208 6 0.32427443454887594 2208 7 0.3397084452243675 2208 8 0.5176120298459992 2208 9 -0.09838343047259457 2208 10 0.08260325952816316 2208 11 0.2839025435633502 2208 12 0.3498533947543905 2208 13 0.5783930315677352 2208 14 0.8297745141468269 2208 15 0.7619296322985392 2208 16 0.483914956886224 2208 17 0.48041873374879174 2208 18 0.7942891632401299 2208 19 -1.5646431030025383 2208 20 -0.1745545379592457 2208 21 -0.23607551072190347 2208 22 0.1572785979722653 2208 23 0.348589292827441 2208 24 0.5168635288352732 2208 25 0.5839591991705646 2209 1 0.13270813640085066 2209 2 -1.447213040948732 2209 3 -0.19666503291155024 2209 4 0.2014575907221925 2209 5 0.033158599316415405 2209 6 0.10339939446959079 2209 7 0.17983893365724413 2209 8 0.3046637491258366 2209 9 -1.9137833002756466 2209 10 -0.4024032989001469 2209 11 -0.03803671081622184 2209 12 0.2994921468069336 2209 13 0.4634862584116277 2209 14 0.6656140415513434 2209 15 0.7654744870900814 2209 16 0.6339394416852429 2209 17 0.47597658880266636 2209 18 0.6964732656099031 2209 19 0.4486154156671205 2209 20 0.3679924641103014 2209 21 0.4198732266591918 2209 22 0.320912161579308 2209 23 0.3925593280972137 2209 24 0.47313503805107615 2209 25 0.42283707637735546 2210 1 0.07143536877674705 2210 2 0.14709618613204256 2210 3 0.030198575461870547 2210 4 0.2795104919434408 2210 5 0.11099562423225251 2210 6 0.11991293548179272 2210 7 0.07902946588904314 2210 8 0.22729942494175503 2210 9 0.38214001733485503 2210 10 -1.5855246241188752 2210 11 -0.49532742997150336 2210 12 -2.3602278743235487 2210 13 -0.861408401765857 2210 14 -0.49918456246157206 2210 15 -0.8818597824235752 2210 16 -0.13453386545379686 2210 17 -0.024180522260515147 2210 18 0.32216086583121495 2210 19 0.2468251466285878 2210 20 0.2632924950558291 2210 21 0.21835486408389926 2210 22 0.08339977555418443 2210 23 0.11016053403500337 2210 24 0.17476792251798656 2210 25 0.04740977804684832 2211 1 -0.387450042775324 2211 2 -0.1815683188522179 2211 3 -1.6273130975906227 2211 4 -0.6812326645071423 2211 5 -0.5620348761565523 2211 6 -0.5397861655737444 2211 7 -0.534767990361992 2211 8 -0.1749555766228852 2211 9 0.0671563152525394 2211 10 0.11140441827976133 2211 11 -0.24703614421511588 2211 12 -0.3488196568975255 2211 13 -0.37595649890150895 2211 14 -0.9037966164541302 2211 15 -0.505733360072405 2211 16 -0.15038566602356696 2211 17 -0.38297235198488977 2211 18 -0.14684132661322025 2211 19 -0.16611136005711558 2211 20 -0.06827061351647501 2211 21 -0.2947714505460084 2211 22 -0.32253730678223036 2211 23 -0.5373340368460688 2211 24 -0.3557541770749495 2211 25 -0.2416530291333956 2212 1 -0.47118977371352244 2212 2 -0.3999928687699139 2212 3 -2.508963224328429 2212 4 -1.5355983559106114 2212 5 -0.6735123419235123 2212 6 -0.532100088174623 2212 7 -0.4124620654052862 2212 8 -0.393322246163855 2212 9 -0.9897548501560536 2212 10 -0.21150491229292356 2212 11 -0.39982044306490394 2212 12 -0.0748408718300766 2212 13 -0.6726552998958439 2212 14 -0.2490285650197066 2212 15 -0.015486096006405603 2212 16 -1.3231532671052122 2212 17 -1.4848361366805563 2212 18 -0.370993807658472 2212 19 -0.29286789022080484 2212 20 -0.27783563721570204 2212 21 -0.17829137586031862 2212 22 -0.4963482280027378 2212 23 -0.1604398767825046 2212 24 -0.21065199799168255 2212 25 -0.08628339603540525 2213 1 -3.3226215951830618 2213 2 -1.500596112726452 2213 3 -2.5305214774703337 2213 4 -1.1752125061820209 2213 5 -0.9171458342656109 2213 6 -0.6797838659711957 2213 7 -0.3974228792232992 2213 8 -0.18270868891549696 2213 9 -0.3818006229752484 2213 10 -0.1677922020816922 2213 11 -0.3900948533425961 2213 12 -0.12731944739596202 2213 13 -0.6258714296082672 2213 14 -0.16802801316094218 2213 15 -0.3057972734793424 2213 16 -1.6739115144934757 2213 17 -1.0618460679163135 2213 18 -0.355061765691012 2213 19 -0.19774424473936136 2213 20 -0.19536402332645508 2213 21 0.05375539799955931 2213 22 0.03819627100388345 2213 23 0.01102258167752268 2213 24 0.038378156137007124 2213 25 0.08955090881025285 2214 1 -0.792890318527725 2214 2 -0.6311879183040325 2214 3 -6.247728348594542 2214 4 -2.1522119539108324 2214 5 -1.2758922049780637 2214 6 -0.7341804732226649 2214 7 -0.5163485120449991 2214 8 -0.43586071274184945 2214 9 -0.15313643279878875 2214 10 -0.2710146769598083 2214 11 -0.8226864355242043 2214 12 -0.45187379755407164 2214 13 -5.142654757284948 2214 14 -4.041564201513882 2214 15 -2.399234096071133 2214 16 -3.054901043824003 2214 17 -3.5876985543445272 2214 18 -1.0997942011830375 2214 19 -0.649072816916231 2214 20 -0.34579219494526114 2214 21 -0.3769345200255998 2214 22 -0.2674338096698037 2214 23 -0.18045062862537975 2214 24 -0.16033997196742433 2214 25 0.21386208265082018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1945.0 3 258.0 4 260.0 5 530.0 6 80.0 7 115.0 8 261.0 9 789.0 10 1985.0 11 4825.0 12 9418.0 13 16228.0 14 24317.0 15 31961.0 16 38133.0 17 41556.0 18 42997.0 19 42951.0 20 43203.0 21 43924.0 22 45258.0 23 47272.0 24 50190.0 25 54187.0 26 60490.0 27 68587.0 28 79174.0 29 95930.0 30 116620.0 31 144184.0 32 184000.0 33 238185.0 34 309944.0 35 409303.0 36 552930.0 37 792732.0 38 1377488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.297509382119884 20.058359292159043 12.94292655301489 23.70120477270618 2 15.753377796451643 20.999607204892552 39.96255567951139 23.284459319144414 3 18.485059292546318 26.78581454329073 29.357422097850762 25.371704066312184 4 12.454136240067127 17.665289630373408 38.06342952465083 31.817144604908638 5 12.853480989289 38.733047188883305 35.001170078742376 13.412301743085317 6 30.188901767096223 38.86959617595732 17.765371853373054 13.176130203573397 7 26.46845026544683 33.54104866592339 22.29006757926174 17.700433489368038 8 24.99215155310702 36.39709196930814 20.954085378222693 17.656671099362143 9 25.53062344934625 16.944650616880452 20.9823678291572 36.5423581046161 10 15.125288194645698 30.076753962273873 33.17802620478243 21.61993163829801 11 32.51099124785352 25.674250947993244 23.335958179023866 18.478799625129373 12 21.333719987667845 27.408915535213048 30.825936321975462 20.431428155143642 13 27.366514318212324 23.686827228561523 26.426382612031052 22.5202758411951 14 20.885784394717888 23.65376755107721 27.80959618208665 27.650851872118253 15 22.922684138668316 30.60476197376302 24.28309569350258 22.189458194066088 16 21.79570256875077 30.411051641461604 25.58988454265836 22.203361247129262 17 20.63737683539413 29.686312628799588 27.3928548428645 22.283455692941782 18 21.027572359846697 28.997412126811223 28.659230332798195 21.315785180543884 19 21.68363521974858 28.72028322895662 28.813748301618467 20.782333249676324 20 22.79047714496276 27.7386821610887 28.39786726300228 21.07297343094626 21 23.050854347647157 27.889605247455634 27.8291920509757 21.230348353921517 22 22.504904537612994 27.81919342594732 28.082512958685964 21.593389077753724 23 22.455813545367402 27.87974449811396 28.019111724617606 21.645330231901028 24 22.332982916526532 27.60738986979429 28.132451514772754 21.927175698906424 25 22.264974286035727 27.9792646005465 28.105273390369035 21.65048772304874 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4728.0 1 4728.0 2 4546.5 3 4365.0 4 4365.0 5 4365.0 6 7227.0 7 10089.0 8 10089.0 9 10089.0 10 15401.0 11 20713.0 12 20713.0 13 20713.0 14 32510.0 15 44307.0 16 44307.0 17 44307.0 18 70463.0 19 96619.0 20 96619.0 21 96619.0 22 138223.5 23 179828.0 24 179828.0 25 179828.0 26 234095.0 27 288362.0 28 288362.0 29 288362.0 30 341834.0 31 395306.0 32 395306.0 33 395306.0 34 439307.5 35 483309.0 36 483309.0 37 483309.0 38 503736.5 39 524164.0 40 524164.0 41 524164.0 42 538687.0 43 553210.0 44 553210.0 45 553210.0 46 571019.5 47 588829.0 48 588829.0 49 588829.0 50 564770.0 51 540711.0 52 540711.0 53 540711.0 54 492647.0 55 444583.0 56 444583.0 57 444583.0 58 387893.5 59 331204.0 60 331204.0 61 331204.0 62 283315.0 63 235426.0 64 235426.0 65 235426.0 66 180237.0 67 125048.0 68 125048.0 69 125048.0 70 93461.5 71 61875.0 72 61875.0 73 61875.0 74 43891.5 75 25908.0 76 25908.0 77 25908.0 78 17658.0 79 9408.0 80 9408.0 81 9408.0 82 6236.0 83 3064.0 84 3064.0 85 3064.0 86 1956.0 87 848.0 88 848.0 89 848.0 90 536.5 91 225.0 92 225.0 93 225.0 94 138.0 95 51.0 96 51.0 97 51.0 98 40.5 99 30.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10154438368451855 2 0.05426158589440108 3 0.05766047693078129 4 0.05229063132892617 5 0.04945487016839595 6 0.06912419226058433 7 0.0649610535355506 8 0.06182361565581502 9 0.06713312591382907 10 0.0672940201640719 11 0.060918585498198986 12 0.06206495703117929 13 0.06272864581343104 14 0.06461915325378453 15 0.0672940201640719 16 0.06238674553166499 17 0.06536328916115772 18 0.1433768887476595 19 0.07057224051276997 20 0.06427725297201849 21 0.06228618662526322 22 0.06721357303895048 23 0.06683144919462372 24 0.07724935189784825 25 0.07057224051276997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4972210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.77538062041868 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1490352187939 44.920621834606536 2 14.516269872368104 17.354311136190397 3 5.0331041241025245 9.025671441612822 4 2.1892072521677473 5.23442787021232 5 1.0945316014410418 3.2713021538607334 6 0.6045686016035027 2.168299096320218 7 0.343464571398079 1.43715078394644 8 0.235278926991755 1.1251109930316685 9 0.16820729320311936 0.9049189476912143 >10 0.6119240717097052 6.070491552984128 >50 0.029895865092896323 1.2128208520434023 >100 0.019462599463638354 2.436212947940503 >500 0.0023543953110931625 0.9806768373956091 >1k 0.0025249983559437923 2.9447181106331226 >5k 1.3648639761720314E-4 0.6379967619055092 >10k+ 3.4121599404300785E-5 0.27526867962521584 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 13496 0.27142860015968756 No Hit GTACTGGTTCACTATCGGTCAGTCA 8612 0.17320266038642776 No Hit GGTATCAACGCAGAGTACTTTTTTT 8588 0.1727199776356992 No Hit TATCAACGCAGAGTACTTTTTTTTT 8058 0.16206073355711043 No Hit GGATACCACGTGTCCCGCCCTACTC 6022 0.12111314687030515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.022356256071244E-5 0.0 0.0 0.0 6 0.0 4.022356256071244E-5 0.0 2.011178128035622E-5 0.0 7 0.0 4.022356256071244E-5 0.0 2.011178128035622E-5 0.0 8 0.0 4.022356256071244E-5 0.0 2.011178128035622E-5 0.0 9 0.0 4.022356256071244E-5 0.0 4.022356256071244E-5 0.0 10 0.0 4.022356256071244E-5 0.0 1.005589064017811E-4 0.0 11 0.0 4.022356256071244E-5 0.0 1.6089425024284977E-4 0.0 12 0.0 4.022356256071244E-5 0.0 1.8100603152320597E-4 0.0 13 0.0 4.022356256071244E-5 0.0 2.4134137536427463E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3925 0.0 12.536797 1 TGTCCCG 1420 0.0 10.967612 11 CGCCCTA 1535 0.0 10.526873 16 CAACGCG 100 2.410425E-5 10.4464035 4 GTCTTAC 455 0.0 10.438907 1 TCTACGC 75 0.0026562342 10.130152 3 GTATCAA 8105 0.0 10.067847 1 CCGTACA 1230 0.0 9.968913 17 CCCCGTA 1240 0.0 9.964672 15 GTCCCGC 1590 0.0 9.922914 12 TCCCGCC 1575 0.0 9.836479 13 CCCGCCC 1605 0.0 9.830274 14 CCCGTAC 1260 0.0 9.731461 16 CCGCCCT 1695 0.0 9.644953 15 GTACTGG 2635 0.0 9.625584 1 TACTGGT 2240 0.0 9.624208 2 TTACGCA 90 0.0011179347 9.49673 4 CACGTGT 1655 0.0 9.23851 7 GTCCTAA 445 0.0 9.179201 1 GCGATAA 450 0.0 9.074287 11 >>END_MODULE