FastQCFastQC Report
Wed 25 May 2016
SRR1294625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4972210
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT360770.7255727332514114No Hit
GGTATCAACGCAGAGTACTTTTTTT251260.5053286164502304No Hit
TATCAACGCAGAGTACTTTTTTTTT225840.4542044684355649No Hit
ACGCAGAGTACTTTTTTTTTTTTTT132530.266541437308561No Hit
GTACTGGTTCACTATCGGTCAGTCA83440.1678127030032923No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71970.14474448987472371No Hit
GGATACCACGTGTCCCGCCCTACTC67800.13635787708081518No Hit
GAGTACTTTTTTTTTTTTTTTTTTT65990.1327176446690707No Hit
ATACAGGGTGACAGCCCCGTACACA50390.101343265871715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61400.014.5130311
GTCTAGG3750.010.144151
CGCCCTA13950.010.06140316
GCGCCTT10100.010.04652813
ACCGGGG7100.010.01733315
TGTCCCG13800.09.97021811
CCCCGTA11300.09.90268115
TATCGCG10200.09.7992387
GTATCAA124450.09.773781
CCGTACA11500.09.73094917
TCGCGCG10200.09.7056199
GTCTTAG5100.09.6966141
CGCGCCT10450.09.61958412
CGGGGGA7650.09.54553717
GTACTGG24950.09.4529661
CCCGTAC11850.09.44353816
TACTGGT21850.09.4348622
CGCCTTT11200.09.398514
ATCGCGC11100.09.3477428
GTATTAG5600.09.3403161
Produced by FastQC (version 0.11.5)