##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4972210 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.367960122360078 31.0 31.0 34.0 28.0 34.0 2 31.449254757944658 31.0 31.0 34.0 28.0 34.0 3 31.60709402861102 31.0 31.0 34.0 28.0 34.0 4 35.214734293201616 37.0 35.0 37.0 32.0 37.0 5 35.381840670446344 37.0 35.0 37.0 33.0 37.0 6 34.98062752779951 37.0 35.0 37.0 32.0 37.0 7 34.804891386325195 37.0 35.0 37.0 32.0 37.0 8 34.84152479480955 37.0 35.0 37.0 32.0 37.0 9 36.277645553989075 39.0 37.0 39.0 32.0 39.0 10 36.045168647341924 39.0 35.0 39.0 30.0 39.0 11 36.2253243527526 39.0 37.0 39.0 31.0 39.0 12 36.170712419628295 39.0 35.0 39.0 31.0 39.0 13 36.05303617506099 39.0 35.0 39.0 30.0 39.0 14 37.11616806208909 40.0 36.0 41.0 31.0 41.0 15 37.18140525038162 40.0 37.0 41.0 31.0 41.0 16 37.22067088879995 40.0 37.0 41.0 31.0 41.0 17 37.26911795760839 40.0 37.0 41.0 31.0 41.0 18 37.27530836388648 40.0 37.0 41.0 31.0 41.0 19 37.27695008859239 40.0 37.0 41.0 31.0 41.0 20 37.352416329961926 40.0 37.0 41.0 31.0 41.0 21 37.18820504363251 40.0 37.0 41.0 30.0 41.0 22 37.19153314119878 40.0 37.0 41.0 30.0 41.0 23 37.1979658944413 40.0 37.0 41.0 30.0 41.0 24 37.130806422094 40.0 36.0 41.0 30.0 41.0 25 36.04060649087629 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4616913686390731 1101 2 0.47379509085968863 1101 3 0.4147093031082214 1101 4 0.33790004791207906 1101 5 0.3394140015508569 1101 6 0.44039819489884735 1101 7 0.5160235897439591 1101 8 0.4850134330434983 1101 9 0.6685739867420324 1101 10 0.7430351874377976 1101 11 0.6983067962460794 1101 12 0.7928572126593494 1101 13 0.7281106864454401 1101 14 0.9581137726237756 1101 15 0.8729979226087892 1101 16 0.954807710206687 1101 17 0.9069522440948958 1101 18 0.931799774831596 1101 19 1.0565912508092907 1101 20 0.8927321109890158 1101 21 1.2053119523732363 1101 22 1.1696054649977086 1101 23 1.116560112003313 1101 24 1.2627018200824054 1101 25 1.1467058364529947 1102 1 0.45835375240686815 1102 2 0.4365784500198693 1102 3 0.34403914703676364 1102 4 0.3060538048932102 1102 5 0.3601730245831547 1102 6 0.40648507484257124 1102 7 0.46674773513789347 1102 8 0.5068923639342131 1102 9 0.6262366471023171 1102 10 0.7664570741267696 1102 11 0.6316101729900296 1102 12 0.7237520741470433 1102 13 0.7186815171874343 1102 14 0.8559111154761183 1102 15 0.818210046439809 1102 16 0.8195441368906202 1102 17 0.8114741392986176 1102 18 0.8328337238703867 1102 19 0.9283491063915434 1102 20 0.8122582371279492 1102 21 1.1630256191600594 1102 22 1.1026275087080748 1102 23 1.0014321802277593 1102 24 1.0868768788788188 1102 25 1.0196435404217183 1103 1 0.3040976584801207 1103 2 0.2924609253817856 1103 3 0.15865297335225748 1103 4 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0.24752282698467454 2207 2 0.24522529910007052 2207 3 0.2455679749509727 2207 4 0.2523634619490309 2207 5 0.16582128649300643 2207 6 0.2963548305715946 2207 7 0.30734480892201077 2207 8 0.3076142331002032 2207 9 0.3786665499812969 2207 10 0.54328568059627 2207 11 0.4254089047384113 2207 12 0.372294244224193 2207 13 0.4659838635343476 2207 14 0.48201410416331214 2207 15 0.4818528117010388 2207 16 0.5637017063595309 2207 17 0.6340374469308259 2207 18 0.528366170623265 2207 19 0.6959472672954874 2207 20 0.4918118429529912 2207 21 0.8764123963284263 2207 22 0.7380482454291055 2207 23 0.6454649252034201 2207 24 0.7797690825542887 2207 25 0.8344294290351186 2208 1 0.11486965219884837 2208 2 0.17753850910813185 2208 3 0.16522078711162536 2208 4 0.1351280887714239 2208 5 0.09783075498460647 2208 6 0.19945939272992064 2208 7 0.26261946436676453 2208 8 0.16759699981739828 2208 9 0.24837342224351744 2208 10 0.30496701501764534 2208 11 -0.10389150347251785 2208 12 -0.2492568788097671 2208 13 0.01699965073274967 2208 14 -0.4452717764687222 2208 15 0.10620667890956526 2208 16 0.26564888085191285 2208 17 0.2322552644376259 2208 18 0.2700204381915725 2208 19 0.45128275936901474 2208 20 0.39814179652813664 2208 21 0.6550746382156092 2208 22 0.6509308701876222 2208 23 0.627980837284241 2208 24 0.6444486058125918 2208 25 0.704049830651087 2209 1 -0.0031127809294702047 2209 2 0.014528822066615987 2209 3 0.11803459576097808 2209 4 -0.5916552693381973 2209 5 -0.09984629828926472 2209 6 0.03572050562232931 2209 7 0.04392619477163606 2209 8 0.0828708506350111 2209 9 0.1553621806115828 2209 10 0.21622011205323588 2209 11 0.2292494492970789 2209 12 0.22578222885532995 2209 13 0.31102254207659286 2209 14 0.2057448285001371 2209 15 -0.38623684573555295 2209 16 -0.3519528719487752 2209 17 0.09670780570714044 2209 18 -0.29921003955349335 2209 19 0.20387873103660326 2209 20 0.17128760739278448 2209 21 0.5001197699185624 2209 22 0.4580658572165035 2209 23 0.45780672315787285 2209 24 0.509571855683852 2209 25 0.5542762178801226 2210 1 -0.1362046453818131 2210 2 -0.12457702413724547 2210 3 -0.06203031169939521 2210 4 0.005319297502580866 2210 5 -0.06762137745042196 2210 6 -0.517149809178072 2210 7 -0.13779343875830818 2210 8 -0.27729850579181203 2210 9 0.012168633535310391 2210 10 0.12437812647572599 2210 11 0.11358730098986314 2210 12 0.07909508031367807 2210 13 0.1700421862342978 2210 14 0.1362521163279169 2210 15 0.04718948046780724 2210 16 0.19853303533342626 2210 17 -0.8803077952143497 2210 18 -1.380730754301382 2210 19 -0.2810721312182949 2210 20 -0.14383799549796805 2210 21 -0.3967784489944677 2210 22 -0.32057434477338376 2210 23 -0.16548724840350104 2210 24 0.24719114443687573 2210 25 0.2125676653493116 2211 1 -0.21756197872987926 2211 2 -0.20652564645871507 2211 3 -0.12089075889046796 2211 4 -0.08659599865614354 2211 5 -0.10492039119162655 2211 6 -0.037793804392194374 2211 7 -0.12636661788722847 2211 8 -0.11125032920563882 2211 9 -0.08495710983073224 2211 10 0.0035536047304915996 2211 11 -0.3468122807007106 2211 12 -0.22790118388708436 2211 13 -0.10033396811353157 2211 14 -0.20518446607137264 2211 15 -0.21224141008966768 2211 16 -0.2358969697325648 2211 17 -0.3352933072737656 2211 18 -0.3449972935764265 2211 19 -0.17518875515931853 2211 20 -0.2929324829641189 2211 21 0.03795369957833117 2211 22 -0.04744813006590931 2211 23 -0.832506601997963 2211 24 -0.1979288550951921 2211 25 -0.8516909491830802 2212 1 -0.24845705477030577 2212 2 -0.2923822483953593 2212 3 -0.2026879912620707 2212 4 -0.10563416875351805 2212 5 -0.1360910174015899 2212 6 -0.13767161033488406 2212 7 -0.21374891945319519 2212 8 -0.360526206116468 2212 9 -0.15304975955846345 2212 10 -0.5658328252055043 2212 11 -0.2475252589674355 2212 12 -0.32227510587582486 2212 13 -0.3198099900328799 2212 14 -0.3722384501803191 2212 15 -0.19801884001639536 2212 16 -0.38831371420936733 2212 17 -0.6248034773248534 2212 18 -0.45633198344568626 2212 19 -0.45880446163513255 2212 20 -0.6408492012968878 2212 21 -0.15600832534524756 2212 22 -0.5683685093593951 2212 23 -0.33082461889882353 2212 24 -0.6186606144794808 2212 25 -0.40111785494610785 2213 1 -0.2295384734086774 2213 2 -0.2327570024767347 2213 3 -0.17906256571745516 2213 4 -0.04601167101900927 2213 5 -0.08506197447483999 2213 6 -0.20721320503713514 2213 7 -0.3566526578139033 2213 8 -0.2073786991463109 2213 9 -0.22172812039175938 2213 10 -0.2131427213229813 2213 11 -0.016509919539210216 2213 12 0.057631984450907225 2213 13 0.09473320153976061 2213 14 -0.5726532505668374 2213 15 -0.18448742458814138 2213 16 -0.2683365440180694 2213 17 -0.19909872889537894 2213 18 -0.1701859421347507 2213 19 -0.3116902596160003 2213 20 -0.4342522262406945 2213 21 -0.1409787636548856 2213 22 -0.45462602169610733 2213 23 -0.4184774720226443 2213 24 -0.7406939877257983 2213 25 -0.5120174104536375 2214 1 -0.37797598125683507 2214 2 -0.18412520710744218 2214 3 -0.21703074351194118 2214 4 -0.09133546003467785 2214 5 -0.15682194961905083 2214 6 -0.18728805464548515 2214 7 -0.12889733523753932 2214 8 -0.1625035198495226 2214 9 -0.19317285368830994 2214 10 -0.1607903067689449 2214 11 -0.059493432486249276 2214 12 -0.2985344498504645 2214 13 -0.2697985267868219 2214 14 -0.2747790282371483 2214 15 -0.1712921136073149 2214 16 -0.3451525391693693 2214 17 -0.5489464058523694 2214 18 -0.387966610825373 2214 19 -0.5107900743217186 2214 20 -0.526376800825652 2214 21 -0.6171274410861756 2214 22 -0.7612684400937937 2214 23 -1.101951025790875 2214 24 -0.688445665168814 2214 25 -1.132329389859109 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 31.0 9 78.0 10 190.0 11 352.0 12 873.0 13 2008.0 14 4147.0 15 7097.0 16 10472.0 17 13876.0 18 16228.0 19 18290.0 20 20105.0 21 21520.0 22 23317.0 23 26000.0 24 28855.0 25 33128.0 26 38244.0 27 43319.0 28 50736.0 29 64067.0 30 82096.0 31 108063.0 32 145341.0 33 198031.0 34 273270.0 35 382366.0 36 551354.0 37 879421.0 38 1929328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.34092787726545 20.310617074980602 12.190271731055951 25.158183316697997 2 15.690186054088626 21.1935738836453 38.78044169494048 24.335798367325594 3 18.016370262408284 26.325361784490696 29.977197297344098 25.681070655756915 4 12.19713091507396 17.494458546711133 38.212896399966205 32.095514138248696 5 12.934370507131963 38.221023911738975 35.06810300311422 13.776502578014846 6 30.49121303733026 38.679612275397915 17.42039235090726 13.408782336364569 7 26.569866875980907 33.54492739512797 22.197260434901033 17.687945293990087 8 25.053965267911327 36.28028989874384 20.680963129416266 17.98478170392857 9 25.579622235934004 15.849631270157058 21.169988198392502 37.40075829551643 10 14.999528601760142 29.68553195020177 33.39691994244702 21.918019505591065 11 32.93727436160144 24.70558710811947 23.456995099776474 18.90014343050262 12 21.654845128022366 26.76160099305349 31.20252671938576 20.381027159538377 13 27.870537894261783 22.69870665000873 26.79280657028553 22.637948885443958 14 21.093783939026803 22.787819036907962 28.427570194957834 27.690826829107394 15 23.1596779338338 29.742409012124742 24.837927124424745 22.259985929616708 16 21.62862487422587 29.84483550262489 26.140786174026946 22.38575344912229 17 20.46249875306906 29.03502830131 27.985152672615467 22.517320273005474 18 20.759338529695935 28.113754968629557 29.623309298346246 21.50359720332827 19 21.37490846553749 27.954207528260795 30.026541725938227 20.64434228026349 20 22.391119100422166 26.95069123339593 29.764737895647382 20.893451770534526 21 22.67338857515558 27.19482868646247 29.073413996503355 21.058368741878596 22 22.06505511497144 27.132651831906323 29.37852130132756 21.42377175179468 23 22.04102163104264 27.28905439557412 29.288368181044795 21.38155579233845 24 21.858489484555157 27.092681121674268 29.3427067641954 21.706122629575177 25 21.84531959705044 27.435452288513428 29.27635125024738 21.44287686418875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1757.0 1 1757.0 2 2210.0 3 2663.0 4 2663.0 5 2663.0 6 7043.5 7 11424.0 8 11424.0 9 11424.0 10 15446.0 11 19468.0 12 19468.0 13 19468.0 14 28069.5 15 36671.0 16 36671.0 17 36671.0 18 62551.0 19 88431.0 20 88431.0 21 88431.0 22 132315.0 23 176199.0 24 176199.0 25 176199.0 26 234445.0 27 292691.0 28 292691.0 29 292691.0 30 348639.5 31 404588.0 32 404588.0 33 404588.0 34 447692.5 35 490797.0 36 490797.0 37 490797.0 38 505417.5 39 520038.0 40 520038.0 41 520038.0 42 535674.0 43 551310.0 44 551310.0 45 551310.0 46 571849.5 47 592389.0 48 592389.0 49 592389.0 50 567059.0 51 541729.0 52 541729.0 53 541729.0 54 494623.0 55 447517.0 56 447517.0 57 447517.0 58 389763.5 59 332010.0 60 332010.0 61 332010.0 62 285899.0 63 239788.0 64 239788.0 65 239788.0 66 182140.0 67 124492.0 68 124492.0 69 124492.0 70 92859.0 71 61226.0 72 61226.0 73 61226.0 74 43035.5 75 24845.0 76 24845.0 77 24845.0 78 16732.5 79 8620.0 80 8620.0 81 8620.0 82 5653.0 83 2686.0 84 2686.0 85 2686.0 86 1695.5 87 705.0 88 705.0 89 705.0 90 416.5 91 128.0 92 128.0 93 128.0 94 80.5 95 33.0 96 33.0 97 33.0 98 19.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0979845983978955 2 0.0 3 0.12332544281114435 4 0.01186595095541017 5 0.022867095315765022 6 0.012871540019427983 7 0.02123804103205617 8 0.019246974685300903 9 1.2067068768213732E-4 10 0.37932830672879864 11 0.06542362450499879 12 0.0014279364709052916 13 1.2067068768213732E-4 14 0.0011664833142606607 15 0.0012871540019427981 16 6.033534384106866E-5 17 4.022356256071244E-5 18 2.8156493792498707E-4 19 7.843594699338925E-4 20 2.212295940839184E-4 21 2.212295940839184E-4 22 0.002996655410773077 23 8.044712512142488E-5 24 0.0 25 0.0014882718147463602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4972210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.31139538811644 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43607759927399 40.065405284577274 2 15.60039671473809 17.257594218007025 3 5.742472520977469 9.528725043395356 4 2.5156318207531094 5.565724251544099 5 1.2941242011440912 3.5789907685405575 6 0.7214978136257869 2.3944230504670467 7 0.42930865847401284 1.6621962666678578 8 0.26718956453500514 1.1822902118059455 9 0.18971674913378603 0.9444148310778243 >10 0.7376183830179719 6.786565752966543 >50 0.03688837377964347 1.3800332723518574 >100 0.022969529191911572 2.665590232163549 >500 0.0028528737400964604 1.0951271011200923 >1k 0.0029260203282100877 3.2427728187791947 >5k 1.8287627051241724E-4 0.6870001943484744 >10k+ 1.463010164099338E-4 1.9631467021872244 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 36077 0.7255727332514114 No Hit GGTATCAACGCAGAGTACTTTTTTT 25126 0.5053286164502304 No Hit TATCAACGCAGAGTACTTTTTTTTT 22584 0.4542044684355649 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 13253 0.266541437308561 No Hit GTACTGGTTCACTATCGGTCAGTCA 8344 0.1678127030032923 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 7197 0.14474448987472371 No Hit GGATACCACGTGTCCCGCCCTACTC 6780 0.13635787708081518 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6599 0.1327176446690707 No Hit ATACAGGGTGACAGCCCCGTACACA 5039 0.101343265871715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.011178128035622E-5 0.0 2 0.0 0.0 0.0 2.011178128035622E-5 0.0 3 0.0 4.022356256071244E-5 0.0 4.022356256071244E-5 0.0 4 0.0 4.022356256071244E-5 0.0 6.033534384106866E-5 0.0 5 0.0 6.033534384106866E-5 0.0 6.033534384106866E-5 0.0 6 0.0 6.033534384106866E-5 0.0 6.033534384106866E-5 0.0 7 0.0 6.033534384106866E-5 0.0 1.005589064017811E-4 0.0 8 0.0 6.033534384106866E-5 0.0 1.2067068768213732E-4 0.0 9 0.0 6.033534384106866E-5 0.0 1.6089425024284977E-4 0.0 10 0.0 6.033534384106866E-5 0.0 1.6089425024284977E-4 0.0 11 0.0 8.044712512142488E-5 0.0 2.2122959408391843E-4 0.0 12 0.0 8.044712512142488E-5 0.0 2.4134137536427463E-4 0.0 13 0.0 1.005589064017811E-4 0.0 2.4134137536427463E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6140 0.0 14.513031 1 GTCTAGG 375 0.0 10.14415 1 CGCCCTA 1395 0.0 10.061403 16 GCGCCTT 1010 0.0 10.046528 13 ACCGGGG 710 0.0 10.017333 15 TGTCCCG 1380 0.0 9.970218 11 CCCCGTA 1130 0.0 9.902681 15 TATCGCG 1020 0.0 9.799238 7 GTATCAA 12445 0.0 9.77378 1 CCGTACA 1150 0.0 9.730949 17 TCGCGCG 1020 0.0 9.705619 9 GTCTTAG 510 0.0 9.696614 1 CGCGCCT 1045 0.0 9.619584 12 CGGGGGA 765 0.0 9.545537 17 GTACTGG 2495 0.0 9.452966 1 CCCGTAC 1185 0.0 9.443538 16 TACTGGT 2185 0.0 9.434862 2 CGCCTTT 1120 0.0 9.3985 14 ATCGCGC 1110 0.0 9.347742 8 GTATTAG 560 0.0 9.340316 1 >>END_MODULE