FastQCFastQC Report
Wed 25 May 2016
SRR1294624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475249
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC60790.24559145362749363No Hit
GTACATGGGAAGCAGTGGTATCAAC46070.1861226890708773No Hit
GAATGGAATGGAATGGAATGGAATG44420.17945669304381096No Hit
GTATCAACGCAGAGTACTTTTTTTT42520.1717806976187042No Hit
CCCATGTACTCTGCGTTGATACCAC42130.17020509855776128No Hit
GGTATCAACGCAGAGTACTTTTTTT30790.12439152586265058No Hit
TATCAACGCAGAGTACTTTTTTTTT26810.10831233544584806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTG402.7692644E-416.6192349
GCGTCGG500.001493632713.30749218
GCGATAA550.00307777812.08647211
TAGACGT600.00589163111.0797144
TATTCCG2151.0913936E-119.7177545
TAGACTG2850.09.6635095
CCGCCCT1106.800081E-59.50496815
CTTAGAC1502.6622365E-79.4968983
TGTACGG800.0045299899.49670510
TAGACAG4300.09.2760395
TCTAGAC2054.2564352E-109.2652663
GTATAGG3300.09.21171951
TTTAGAC2501.8189894E-129.1170223
CTAGGAC2201.6916601E-109.065223
CGCCCTA1054.470421E-49.0523516
TAGAACT3900.09.0098764
CCTATAC2750.08.9788853
TATCGGT2651.8189894E-128.96713213
CTAATAC3500.08.9542173
GCCTAAG1701.6867853E-78.9407861
Produced by FastQC (version 0.11.5)