##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2475249 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.638778563287975 31.0 30.0 33.0 25.0 34.0 2 29.84480874449399 31.0 30.0 34.0 25.0 34.0 3 29.752251187658292 31.0 30.0 34.0 25.0 34.0 4 33.46155922091071 35.0 33.0 37.0 27.0 37.0 5 33.745663567584515 35.0 35.0 37.0 28.0 37.0 6 33.883664633335876 35.0 35.0 37.0 28.0 37.0 7 33.80257137766746 36.0 35.0 37.0 28.0 37.0 8 33.699585375047114 36.0 35.0 37.0 27.0 37.0 9 34.76326583709356 38.0 34.0 39.0 27.0 39.0 10 34.871333348685326 38.0 34.0 39.0 27.0 39.0 11 35.092435952908176 38.0 35.0 39.0 27.0 39.0 12 34.8647412846142 38.0 34.0 39.0 27.0 39.0 13 34.718974131491414 38.0 34.0 39.0 27.0 39.0 14 35.54300718836772 39.0 34.0 41.0 25.0 41.0 15 35.52186790096673 39.0 34.0 41.0 26.0 41.0 16 35.629087821063656 39.0 34.0 41.0 27.0 41.0 17 35.59004286033446 39.0 34.0 41.0 26.0 41.0 18 35.48103362530396 39.0 34.0 41.0 25.0 41.0 19 35.77267418348619 39.0 34.0 41.0 27.0 41.0 20 35.87404459106942 39.0 34.0 41.0 27.0 41.0 21 35.918696260457025 39.0 34.0 41.0 27.0 41.0 22 35.90152950268842 39.0 34.0 41.0 27.0 41.0 23 35.876363549687326 39.0 34.0 41.0 27.0 41.0 24 35.89497218259658 39.0 34.0 41.0 27.0 41.0 25 34.49926411443859 38.0 32.0 40.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0337480690873306 1101 2 1.063788798059658 1101 3 1.3910353121360899 1101 4 1.1674490490355254 1101 5 0.9019605351022975 1101 6 0.8904801079180515 1101 7 0.8836554488202637 1101 8 0.984358940164519 1101 9 1.3623594625554176 1101 10 1.32642825093275 1101 11 1.1345456778477114 1101 12 1.3803353133963867 1101 13 1.3459615175675879 1101 14 1.3765260527949081 1101 15 1.535700867851581 1101 16 1.4805914654899865 1101 17 1.5551419616737405 1101 18 1.4967927272357144 1101 19 1.2655306874602772 1101 20 1.3229744925460452 1101 21 1.161873097232423 1101 22 1.1177073501881267 1101 23 1.18561240830131 1101 24 1.1621599851143216 1101 25 1.1275289428450819 1102 1 0.9103227754298722 1102 2 0.9519627131119002 1102 3 1.2437522072856808 1102 4 1.1679976923760407 1102 5 0.7955851217872763 1102 6 0.8374945330175763 1102 7 0.7800363130290364 1102 8 0.8803221246415092 1102 9 1.1066390227700253 1102 10 1.044051430247606 1102 11 0.8676706747935228 1102 12 1.0658784503124394 1102 13 1.0854823187023683 1102 14 1.3353750479335247 1102 15 1.4224933122997712 1102 16 1.265621454290553 1102 17 1.3319152458083536 1102 18 1.426759226673525 1102 19 1.0817717298026537 1102 20 0.35306084945209193 1102 21 0.661607127379817 1102 22 0.8449609748992017 1102 23 0.8445590486271755 1102 24 0.9339287090676436 1102 25 0.8976395200223166 1103 1 0.7030220422948297 1103 2 0.7713291560451694 1103 3 1.0029379133928167 1103 4 0.8814677772991146 1103 5 0.6318614980038504 1103 6 0.5631176144788341 1103 7 0.47258723143898607 1103 8 0.6639898687783443 1103 9 0.7036106950007337 1103 10 0.6434852941431402 1103 11 0.6821266513446176 1103 12 0.9389290748930748 1103 13 0.6981073213671962 1103 14 0.7652537768056362 1103 15 0.9489244542797195 1103 16 0.9422250135099759 1103 17 0.7249613992372019 1103 18 1.0084250489419588 1103 19 0.8714802712493892 1103 20 -0.023540569039433024 1103 21 0.34502648946151737 1103 22 -0.29971513451974374 1103 23 0.38696086290072884 1103 24 0.5383596878558663 1103 25 0.5302194752124052 1104 1 0.2689170637973035 1104 2 0.23126754320673015 1104 3 0.5110638263252625 1104 4 0.29431081324877084 1104 5 0.055845802199705474 1104 6 0.024055016134987284 1104 7 0.09900774149559766 1104 8 0.2490007740761797 1104 9 0.4752518678719113 1104 10 0.3388713577396203 1104 11 0.09757768662969113 1104 12 0.2833797724071303 1104 13 0.24609832940339516 1104 14 0.5470527739664917 1104 15 0.5593373985460985 1104 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0.3501921994060382 2207 8 0.43689449685910375 2207 9 -1.339071034638856 2207 10 -0.1796265485891908 2207 11 0.15257915776481212 2207 12 0.47984835784815516 2207 13 0.702560063927784 2207 14 0.9469513044707583 2207 15 0.9426953658428943 2207 16 1.0150912864898558 2207 17 0.8428989375615643 2207 18 0.9191448516142842 2207 19 -0.4726254566304462 2207 20 0.348067679136868 2207 21 -0.2420300664121271 2207 22 0.3303135544955751 2207 23 -0.44612959924885587 2207 24 -1.599182837523493 2207 25 -0.002869749138092459 2208 1 0.5384555237516011 2208 2 0.15659382419695334 2208 3 0.6663323608217055 2208 4 0.5870785861916943 2208 5 0.4041364756647212 2208 6 0.3656371539365537 2208 7 0.44007988871612014 2208 8 0.6255056824852474 2208 9 -0.07599989728758061 2208 10 0.17908927788762696 2208 11 0.35607912664516306 2208 12 0.21120394893537764 2208 13 0.599071591652681 2208 14 0.8997932082107454 2208 15 0.8674037448025302 2208 16 0.5747351580790223 2208 17 0.560070761801029 2208 18 0.7735024319131796 2208 19 -1.4951884277228729 2208 20 -0.1919903800785292 2208 21 -0.27787120887572314 2208 22 0.1731209874449533 2208 23 0.34609929354108715 2208 24 0.5020560507901095 2208 25 0.6684270869580899 2209 1 0.10464348679602153 2209 2 -1.4231478616608868 2209 3 -0.19776837549994397 2209 4 0.17813655490645175 2209 5 0.22736172727394433 2209 6 0.22802664882160428 2209 7 0.2086983407990317 2209 8 0.3899203017052528 2209 9 -2.0667366108434706 2209 10 -0.3396698363777517 2209 11 -0.01217816689111828 2209 12 0.36602032346593916 2209 13 0.5204479406187659 2209 14 0.7466088712770613 2209 15 0.8091505539703334 2209 16 0.6054396639005049 2209 17 0.49730596678413264 2209 18 0.8018428812593044 2209 19 0.5293140611687264 2209 20 0.43175630137650955 2209 21 0.523579290231794 2209 22 0.5416961925870112 2209 23 0.44708035231280263 2209 24 0.5370381120796281 2209 25 0.5923993486223651 2210 1 0.24717113381491274 2210 2 0.17531923760014934 2210 3 0.09461475144315656 2210 4 0.2681747422046925 2210 5 0.11810239749166129 2210 6 0.13674250364253737 2210 7 0.17956307144308425 2210 8 0.2452719046721512 2210 9 0.41356207685795 2210 10 -1.591849779739789 2210 11 -0.47972255086360605 2210 12 -2.246945696516846 2210 13 -0.7206367685114685 2210 14 -0.44506553605816634 2210 15 -0.6990161963121153 2210 16 -0.13884755712496855 2210 17 -0.03648805361149954 2210 18 0.3695887709780976 2210 19 0.23215407244995134 2210 20 0.31093646936971453 2210 21 0.2877678943119122 2210 22 0.16341372137926413 2210 23 0.24022512792723916 2210 24 0.15446750366032092 2210 25 0.17272237596610296 2211 1 -0.30399152189099254 2211 2 -0.12988060742159746 2211 3 -1.471041550742246 2211 4 -0.7473871330454287 2211 5 -0.641485929721128 2211 6 -0.5415664116728109 2211 7 -0.64273551375517 2211 8 -0.3049488002271801 2211 9 0.033833764104613806 2211 10 -0.02932885589517298 2211 11 -0.39162015425689845 2211 12 -0.525001327218142 2211 13 -0.3287966186936373 2211 14 -1.0022723105521365 2211 15 -0.4855558016773287 2211 16 -0.23540792755579076 2211 17 -0.48251171785354074 2211 18 -0.20128421881127423 2211 19 -0.243333056182081 2211 20 -0.22696116234296682 2211 21 -0.33132286625653506 2211 22 -0.31585615682170953 2211 23 -0.5571816692056828 2211 24 -0.3170087884178727 2211 25 -0.4270824272833025 2212 1 -0.3083939506916309 2212 2 -0.33638153750139566 2212 3 -2.508413602225204 2212 4 -1.4241714433344406 2212 5 -0.7237791721209348 2212 6 -0.5142582266009512 2212 7 -0.24042999951295485 2212 8 -0.26705033610974027 2212 9 -0.8610306158991392 2212 10 -0.29837561267856216 2212 11 -0.2787785709223698 2212 12 0.10861101653156169 2212 13 -0.24606477422724993 2212 14 0.09159173218330352 2212 15 0.17147333962096667 2212 16 -1.0385462357815172 2212 17 -1.413817889232547 2212 18 -0.23999975395222606 2212 19 0.04246825446728053 2212 20 -0.10450864924327874 2212 21 0.11434145990688904 2212 22 -0.28707507118197384 2212 23 0.03767096365535849 2212 24 -0.025582868306919693 2212 25 0.06888040522596839 2213 1 -3.007973306232575 2213 2 -1.3799373248586306 2213 3 -2.304681185768292 2213 4 -1.1013642368218228 2213 5 -0.8793847414082236 2213 6 -0.5533725867149357 2213 7 -0.26848467951325716 2213 8 -0.09696361834592437 2213 9 -0.3248266581374324 2213 10 -0.06275566286866763 2213 11 -0.4865834531192448 2213 12 -0.12221281167315823 2213 13 -0.54638205277476 2213 14 -0.18186477098759468 2213 15 -0.4479477356868671 2213 16 -1.4277177589453132 2213 17 -1.0723375457629274 2213 18 -0.41154663332212493 2213 19 -0.12306951282903356 2213 20 -0.2631550093270292 2213 21 -0.0376113432556906 2213 22 -0.040625983145204714 2213 23 -0.0027012670074668677 2213 24 0.10815929893799137 2213 25 0.01563722888788277 2214 1 -0.887278931217697 2214 2 -0.6841911752240293 2214 3 -6.283737756979221 2214 4 -2.0353004171641302 2214 5 -1.1149759242499897 2214 6 -0.8988196932873365 2214 7 -0.6137475993637622 2214 8 -0.3692472361421082 2214 9 -0.1902001495321386 2214 10 0.05716358062677784 2214 11 -0.7314152153606699 2214 12 -0.40668518664043063 2214 13 -5.191273028518054 2214 14 -4.0424761875888 2214 15 -2.2306050234109236 2214 16 -2.94386132341959 2214 17 -3.5095777799984518 2214 18 -1.2786861153378837 2214 19 -0.7171910106033224 2214 20 -0.15210886445331084 2214 21 -0.30878418717009026 2214 22 -0.26492494289193047 2214 23 -0.2801507421199432 2214 24 -0.24891150731781408 2214 25 0.14531211998264837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 969.0 3 131.0 4 125.0 5 299.0 6 33.0 7 31.0 8 70.0 9 212.0 10 578.0 11 1555.0 12 3279.0 13 5917.0 14 9262.0 15 12522.0 16 15536.0 17 17376.0 18 18787.0 19 19646.0 20 20310.0 21 20556.0 22 21717.0 23 22933.0 24 24206.0 25 26451.0 26 29720.0 27 33451.0 28 38694.0 29 47002.0 30 57245.0 31 69658.0 32 90241.0 33 117001.0 34 153355.0 35 203797.0 36 278141.0 37 404157.0 38 710286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.0154864456193 22.734914222788216 12.852002816305616 22.397596515286867 2 13.99097708376534 22.465169692023558 42.33583664487537 21.208016579335734 3 16.99195096198778 29.69546081470235 30.732756738926913 22.579831484382957 4 11.136571896762959 17.51194543801691 40.76141986299681 30.590062802223322 5 12.701800904190927 38.5686944606685 35.55034589243887 13.179158742701699 6 29.080204469082215 40.84704114932194 18.05853824981969 12.01421613177615 7 23.411042686848702 34.25664987211046 26.428847773242364 15.903459667798478 8 22.26249044949044 39.60039455226807 22.534796195157842 15.602318803083653 9 23.6568125082118 16.63394075376732 23.597626916507483 36.11161982151339 10 14.460958803042523 30.722326321300287 34.12052449764615 20.69619037801104 11 31.55907523517308 27.664681793743 23.489333834604704 17.286909136479213 12 18.850535495338747 28.995739564885024 34.16550409893556 17.988220840840672 13 24.893772271844348 26.044520444112955 28.28291280857179 20.778794475470903 14 18.85404294438349 23.201642290765996 30.01134370788052 27.93297105696999 15 22.132850792295514 31.316331200385516 24.740981867517906 21.809836139801064 16 21.044729418670364 32.721490382322 26.417587766213334 19.816192432794296 17 18.609332217012998 31.581381490885967 30.53954090450246 19.269745387598576 18 19.032607050929855 31.469805484056558 30.679005402816067 18.81858206219752 19 19.78947972723572 29.122847020668356 30.846807204877642 20.240866047218283 20 21.609871775902537 29.184100502983863 29.145412546217354 20.060615174896245 21 22.110993787413772 30.441955493174568 28.160180265255764 19.2868704541559 22 19.669187206696144 29.87401881672612 30.94414330207993 19.512650674497806 23 19.705189265629226 30.52017171823752 30.213936279581027 19.560702736552223 24 19.771602542331763 28.42588100205392 30.299597302411335 21.50291915320298 25 21.072890493449577 28.75017434773732 28.996868815708883 21.18006634310422 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2475.0 1 2475.0 2 2533.5 3 2592.0 4 2592.0 5 2592.0 6 4343.0 7 6094.0 8 6094.0 9 6094.0 10 10029.0 11 13964.0 12 13964.0 13 13964.0 14 23530.0 15 33096.0 16 33096.0 17 33096.0 18 49856.5 19 66617.0 20 66617.0 21 66617.0 22 90361.0 23 114105.0 24 114105.0 25 114105.0 26 144243.0 27 174381.0 28 174381.0 29 174381.0 30 206705.5 31 239030.0 32 239030.0 33 239030.0 34 267380.5 35 295731.0 36 295731.0 37 295731.0 38 315586.0 39 335441.0 40 335441.0 41 335441.0 42 321198.0 43 306955.0 44 306955.0 45 306955.0 46 291359.5 47 275764.0 48 275764.0 49 275764.0 50 246272.5 51 216781.0 52 216781.0 53 216781.0 54 187134.5 55 157488.0 56 157488.0 57 157488.0 58 131920.0 59 106352.0 60 106352.0 61 106352.0 62 86544.0 63 66736.0 64 66736.0 65 66736.0 66 50911.5 67 35087.0 68 35087.0 69 35087.0 70 25746.0 71 16405.0 72 16405.0 73 16405.0 74 11504.0 75 6603.0 76 6603.0 77 6603.0 78 4526.5 79 2450.0 80 2450.0 81 2450.0 82 1624.0 83 798.0 84 798.0 85 798.0 86 500.0 87 202.0 88 202.0 89 202.0 90 139.0 91 76.0 92 76.0 93 76.0 94 44.5 95 13.0 96 13.0 97 13.0 98 13.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10124233965956556 2 0.05357036807206063 3 0.05708516597724108 4 0.05219676889072574 5 0.04904557076883982 6 0.06952835856109829 7 0.06585196075223139 8 0.06298356246179677 9 0.06762955969278242 10 0.06641756041513398 11 0.06217556294336449 12 0.06294316248587516 13 0.0627815625821887 14 0.06589236072815301 15 0.06775075962054726 16 0.0636299620765426 17 0.06649836036697722 18 0.1444299139197713 19 0.07102315767019803 20 0.06508436120972072 21 0.06306436241364 22 0.06888195894635246 23 0.06714475998172305 24 0.07793155355279409 25 0.07045755800729542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2475249.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.29853300279656 #Duplication Level Percentage of deduplicated Percentage of total 1 89.7185520568278 71.1454956124148 2 7.582726393446137 12.025981583237302 3 1.3781187630358336 3.278483886371107 4 0.45441270316521193 1.4413704295534624 5 0.22605445081977146 0.8962893164380351 6 0.13600752102054495 0.6471118136565739 7 0.09503773812881078 0.5275447249463047 8 0.06429532995868166 0.407882027572337 9 0.046750252212157783 0.33365037761413713 >10 0.2633652359888261 3.7929025152895006 >50 0.018240972035162076 1.0056643973039026 >100 0.014798106717987838 2.3296762885775597 >500 9.227681117358242E-4 0.5865315251724527 >1k 6.664436362534946E-4 1.3342901108405043 >5k 5.126489509642266E-5 0.24712539101210854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCCATTCCATTCCATTCCATTCC 6079 0.24559145362749363 No Hit GTACATGGGAAGCAGTGGTATCAAC 4607 0.1861226890708773 No Hit GAATGGAATGGAATGGAATGGAATG 4442 0.17945669304381096 No Hit GTATCAACGCAGAGTACTTTTTTTT 4252 0.1717806976187042 No Hit CCCATGTACTCTGCGTTGATACCAC 4213 0.17020509855776128 No Hit GGTATCAACGCAGAGTACTTTTTTT 3079 0.12439152586265058 No Hit TATCAACGCAGAGTACTTTTTTTTT 2681 0.10831233544584806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.07999518432287E-5 0.0 0.0 0.0 6 0.0 8.07999518432287E-5 0.0 4.039997592161435E-5 0.0 7 0.0 8.07999518432287E-5 0.0 4.039997592161435E-5 0.0 8 0.0 8.07999518432287E-5 0.0 4.039997592161435E-5 0.0 9 0.0 8.07999518432287E-5 0.0 4.039997592161435E-5 0.0 10 0.0 8.07999518432287E-5 0.0 4.039997592161435E-5 0.0 11 0.0 8.07999518432287E-5 0.0 8.07999518432287E-5 0.0 12 0.0 8.07999518432287E-5 0.0 8.07999518432287E-5 0.0 13 0.0 8.07999518432287E-5 0.0 1.2119992776484305E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGTG 40 2.7692644E-4 16.619234 9 GCGTCGG 50 0.0014936327 13.307492 18 GCGATAA 55 0.003077778 12.086472 11 TAGACGT 60 0.005891631 11.079714 4 TATTCCG 215 1.0913936E-11 9.717754 5 TAGACTG 285 0.0 9.663509 5 CCGCCCT 110 6.800081E-5 9.504968 15 CTTAGAC 150 2.6622365E-7 9.496898 3 TGTACGG 80 0.004529989 9.496705 10 TAGACAG 430 0.0 9.276039 5 TCTAGAC 205 4.2564352E-10 9.265266 3 GTATAGG 330 0.0 9.2117195 1 TTTAGAC 250 1.8189894E-12 9.117022 3 CTAGGAC 220 1.6916601E-10 9.06522 3 CGCCCTA 105 4.470421E-4 9.05235 16 TAGAACT 390 0.0 9.009876 4 CCTATAC 275 0.0 8.978885 3 TATCGGT 265 1.8189894E-12 8.967132 13 CTAATAC 350 0.0 8.954217 3 GCCTAAG 170 1.6867853E-7 8.940786 1 >>END_MODULE