FastQCFastQC Report
Wed 25 May 2016
SRR1294624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475249
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115080.46492292290593795No Hit
GGTATCAACGCAGAGTACTTTTTTT88950.3593577858227596No Hit
TATCAACGCAGAGTACTTTTTTTTT75890.3065954172691313No Hit
ATTCCATTCCATTCCATTCCATTCC59430.2400970569021541No Hit
GAATGGAATGGAATGGAATGGAATG51330.20737307640564645No Hit
CCCATGTACTCTGCGTTGATACCAC48510.1959802831957512No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46520.18794068798734995No Hit
GTACATGGGAAGCAGTGGTATCAAC45980.18575908928758278No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27390.1106555340493017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA402.723407E-416.65565110
AGCGTCG456.8488927E-414.7535817
GTACCGT657.896562E-411.7150536
GTGCGTC600.005921771311.0721211
AGGGCCG803.708702E-410.70720410
TCGCGCG1252.185534E-710.6596169
CGTTGGA750.002685214410.11653714
GCGTCGG750.002685214410.11653718
TATCGCG1252.5082081E-69.8992197
CGCGCCT1151.0471194E-59.896811512
ATCGCGC1401.0314016E-69.518488
CCGTTGC800.0044489219.5175159
CTAGTAC1901.0531949E-99.4997993
GGTATCA34500.09.3980231
TGTACTG3150.09.3673935
GTACTAG1951.6570993E-99.26446151
TATTCCG1651.0347867E-79.23004155
TCAGGAC2401.0913936E-119.1039723
GTGTAGG2451.6370905E-118.9261251
GCGCCTT1606.906739E-78.89148713
Produced by FastQC (version 0.11.5)