FastQCFastQC Report
Wed 25 May 2016
SRR1294623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917418
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC376700.9616027699877828No Hit
CCCATGTACTCTGCGTTGATACCAC364640.930817186218065No Hit
GAGTACATGGGAAGCAGTGGTATCA164110.4189238932378419No Hit
CATGTACTCTGCGTTGATACCACTG129740.33118753219595154No Hit
GTATCAACGCAGAGTACTTTTTTTT112330.28674499376885487No Hit
GCGTTGATACCACTGCTTCCCATGT112120.2862089263897802No Hit
GTATCAACGCAGAGTACATGGGAAG104070.2656596768585839No Hit
ACGCAGAGTACATGGGAAGCAGTGG88730.2265012311680806No Hit
TATCAACGCAGAGTACATGGGAAGC84560.2158564646407404No Hit
ACTCTGCGTTGATACCACTGCTTCC75480.192677932250273No Hit
GGTATCAACGCAGAGTACATGGGAA70630.18029732849545288No Hit
TATCAACGCAGAGTACTTTTTTTTT66890.17075022374431323No Hit
GGTATCAACGCAGAGTACTTTTTTT66880.17072469672626203No Hit
GCTTCCCATGTACTCTGCGTTGATA66530.16983125109447092No Hit
GTGGTATCAACGCAGAGTACATGGG49210.12561845582983486No Hit
CAGTGGTATCAACGCAGAGTACATG45300.11563739177182521No Hit
ATACCACTGCTTCCCATGTACTCTG42360.10813244846477961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGC551.9671406E-413.81337910
TGGACCG701.09500426E-412.2103525
GTCCGGA550.003071995712.0897951
TACGCTG658.0499204E-411.6885425
CAGTGCG600.00589359111.0794819
TAGACTG5200.010.9580085
TACCGTA803.7846787E-410.6840582
TGTCCCG4550.010.64424311
TAGGACG909.553772E-510.5521564
GGACCGT1002.4098154E-510.4465026
GTCCCGC4850.010.38685612
TCCCGCC5050.010.35193113
CGCCCTA5100.09.50519816
CACACCG1106.865966E-59.4969415
TATACCG800.0045300559.4969415
AGTGCGA800.00453100969.49669810
CCCGCCC5250.09.4143114
CTACACT5200.09.3143074
GTCTTAC3800.09.2491471
TAGGACC2501.8189894E-129.1170634
Produced by FastQC (version 0.11.5)