##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294623_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3917418 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.996542365404967 31.0 30.0 33.0 25.0 34.0 2 30.210305869835693 31.0 30.0 34.0 25.0 34.0 3 30.183330448780293 31.0 30.0 34.0 25.0 34.0 4 33.82481879646237 35.0 35.0 37.0 28.0 37.0 5 34.194671592360066 37.0 35.0 37.0 30.0 37.0 6 34.227190971195824 37.0 35.0 37.0 30.0 37.0 7 34.19705453949515 37.0 35.0 37.0 30.0 37.0 8 33.98045115430623 37.0 35.0 37.0 28.0 37.0 9 35.15922451982403 39.0 35.0 39.0 27.0 39.0 10 35.308019465882886 39.0 35.0 39.0 28.0 39.0 11 35.513110421200906 39.0 35.0 39.0 29.0 39.0 12 35.322480521608874 39.0 35.0 39.0 27.0 39.0 13 35.15580798372806 39.0 35.0 39.0 27.0 39.0 14 36.090292636629535 39.0 36.0 41.0 27.0 41.0 15 36.05636263477627 39.0 35.0 41.0 27.0 41.0 16 36.16644304998854 39.0 36.0 41.0 27.0 41.0 17 36.174835567713224 39.0 36.0 41.0 27.0 41.0 18 36.06454404406168 39.0 35.0 41.0 27.0 41.0 19 36.32683007021462 39.0 36.0 41.0 27.0 41.0 20 36.450740513266645 39.0 36.0 41.0 27.0 41.0 21 36.48975779454733 39.0 36.0 41.0 27.0 41.0 22 36.507221593406676 39.0 36.0 41.0 27.0 41.0 23 36.49925563215363 39.0 36.0 41.0 27.0 41.0 24 36.50974213116905 39.0 36.0 41.0 27.0 41.0 25 35.358324284005434 39.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9042685044829071 1101 2 0.9751980318737843 1101 3 1.248676701079546 1101 4 1.0164012383951402 1101 5 0.7594142798631935 1101 6 0.6753939842394701 1101 7 0.7522706768491716 1101 8 0.8753835421662544 1101 9 1.1523521609299223 1101 10 1.07183101374563 1101 11 0.9313185063138789 1101 12 1.0999962703675905 1101 13 1.1322119398644475 1101 14 1.220214476100665 1101 15 1.392028794564105 1101 16 1.3682780707986097 1101 17 1.253799289315097 1101 18 1.2126145417943945 1101 19 1.2169306963940656 1101 20 1.163822284459691 1101 21 1.0384237698843108 1101 22 1.0018066925889073 1101 23 0.9979114431818488 1101 24 1.0261225809427756 1101 25 1.0094031544861295 1102 1 0.8710097806584436 1102 2 0.8358870546237895 1102 3 1.0578795948359527 1102 4 0.9681938616116383 1102 5 0.7181795496210555 1102 6 0.692627768777335 1102 7 0.6564588496550812 1102 8 0.751757410491372 1102 9 1.0954465501381563 1102 10 0.9376766674959924 1102 11 0.7612669713798397 1102 12 0.9813921250837367 1102 13 0.9893125314301869 1102 14 1.1540919484905459 1102 15 1.2402198449632635 1102 16 1.1770044488673221 1102 17 1.2300461956085442 1102 18 1.2994531509329903 1102 19 1.011712978382242 1102 20 0.24477969541215572 1102 21 0.5793529925235248 1102 22 0.6942638050888306 1102 23 0.79851244346942 1102 24 0.88328213393428 1102 25 0.906803690133728 1103 1 0.6627119604901424 1103 2 0.7012506292100262 1103 3 0.9944463157468029 1103 4 0.8052238865036756 1103 5 0.6537553863744279 1103 6 0.49062888607458177 1103 7 0.46187393271415544 1103 8 0.5601944147150277 1103 9 0.7619944209122167 1103 10 0.7584755755461856 1103 11 0.6586673509756622 1103 12 0.860170721791448 1103 13 0.7321322273029764 1103 14 0.6815009809004309 1103 15 0.9109847268229387 1103 16 0.9331873050803736 1103 17 0.8355746290732 1103 18 0.9403837328043494 1103 19 0.7410003148535438 1103 20 -0.10074585648227696 1103 21 0.3484290116845088 1103 22 -0.21087337985692756 1103 23 0.35015178627044463 1103 24 0.5061518504950584 1103 25 0.5483185273841613 1104 1 0.2936849388992009 1104 2 0.29923188816340485 1104 3 0.6282107182135199 1104 4 0.41321025713465787 1104 5 0.2724226391408706 1104 6 0.17620804180439364 1104 7 0.1903580407896044 1104 8 0.28046693244119325 1104 9 0.5003533444087793 1104 10 0.49927111802831803 1104 11 0.22460147550061293 1104 12 0.3951793942657602 1104 13 0.383894549697132 1104 14 0.647818860034981 1104 15 0.7019594796163986 1104 16 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0.28238757789829805 2207 7 0.24488239755518748 2207 8 0.28251540679525533 2207 9 -1.2911711479559358 2207 10 -0.24569115081934 2207 11 0.12004391624165578 2207 12 0.4615546277308411 2207 13 0.4802759484207968 2207 14 0.8030012686235253 2207 15 0.6831386179844472 2207 16 0.6650047344850734 2207 17 0.6246650105047138 2207 18 0.6711434508387626 2207 19 -0.463827996814949 2207 20 0.14746935084449575 2207 21 -0.40323145493803736 2207 22 0.2049436069124866 2207 23 -0.6354729315571817 2207 24 -1.5751615528693605 2207 25 -0.2597213390570374 2208 1 0.5617096377492565 2208 2 0.2570430877001968 2208 3 0.6810736733984513 2208 4 0.518257578283503 2208 5 0.4536507994683632 2208 6 0.32138991304145037 2208 7 0.37483351561621703 2208 8 0.4847152598064426 2208 9 -0.19992620069620415 2208 10 0.18499010430799245 2208 11 0.3240651592355306 2208 12 0.3623908943375298 2208 13 0.6641163854906367 2208 14 0.8392753599080649 2208 15 0.7273312085142791 2208 16 0.544695198598923 2208 17 0.5047974738397514 2208 18 0.7745890237954711 2208 19 -1.2662805155283507 2208 20 -0.02495050978135538 2208 21 -0.21023982340562242 2208 22 0.23715089734314176 2208 23 0.38218810266361913 2208 24 0.4609324682002267 2208 25 0.6450098185665283 2209 1 0.21865358816940272 2209 2 -1.3425283578819034 2209 3 -0.1944934580157458 2209 4 0.1580620235353507 2209 5 0.150521221262494 2209 6 0.21409088848712798 2209 7 0.1990692748675329 2209 8 0.37041188241038725 2209 9 -1.953542204132063 2209 10 -0.3028922005303585 2209 11 0.020201032625536186 2209 12 0.35703170601987466 2209 13 0.5028856823341883 2209 14 0.7086137778327739 2209 15 0.744107383193807 2209 16 0.7022079978567888 2209 17 0.4770029458818783 2209 18 0.7161879094801975 2209 19 0.4905054064838268 2209 20 0.3515522358063947 2209 21 0.3740961672271652 2209 22 0.3116732118906569 2209 23 0.4060793721983629 2209 24 0.5325644861652421 2209 25 0.5265040330119177 2210 1 0.16042475072989504 2210 2 0.06900242072655871 2210 3 0.06535700907074116 2210 4 0.11417192008192245 2210 5 0.14778547848150936 2210 6 0.10205163324398825 2210 7 0.011875317631862004 2210 8 0.09217449670858002 2210 9 0.17811419718049137 2210 10 -1.5176213988420955 2210 11 -0.6072723378269913 2210 12 -2.4221106579637848 2210 13 -0.8536192661393827 2210 14 -0.5369137400498047 2210 15 -0.7899047688948073 2210 16 -0.18269811336866582 2210 17 -0.0879209282307798 2210 18 0.27560677325183747 2210 19 0.14613525172047304 2210 20 0.11246152319072422 2210 21 0.10576254808277241 2210 22 0.06829870332513366 2210 23 0.05125621040062356 2210 24 0.03830466273734601 2210 25 0.06563887943345748 2211 1 -0.25842212691296496 2211 2 -0.029752101947497778 2211 3 -1.4611924506503868 2211 4 -0.5287310856663794 2211 5 -0.5057886936065614 2211 6 -0.37495307676400813 2211 7 -0.33459357919621624 2211 8 -0.06104245525018115 2211 9 0.21040285686630966 2211 10 0.1397004194888467 2211 11 -0.18832592795225622 2211 12 -0.230218870166901 2211 13 -0.20096381975383792 2211 14 -0.6278026309366425 2211 15 -0.32698549604022986 2211 16 -0.08877061203251913 2211 17 -0.17485505175473293 2211 18 -0.05281246124604877 2211 19 -0.009096014431101196 2211 20 -0.011486363821063605 2211 21 -0.11993970669175269 2211 22 -0.19921497868705274 2211 23 -0.447900134252059 2211 24 -0.27211179816904973 2211 25 -0.01011877694628538 2212 1 -0.3162744805303994 2212 2 -0.3490004017786674 2212 3 -2.345794317286405 2212 4 -1.2089104860943607 2212 5 -0.5052598082332054 2212 6 -0.34793479744398326 2212 7 -0.22398809972204248 2212 8 -0.20693681144835097 2212 9 -0.9077437837857119 2212 10 -0.06616733881509873 2212 11 -0.19212178012291758 2212 12 0.14317650246390912 2212 13 -0.25871203948407384 2212 14 0.009586314229139248 2212 15 0.1256993929814314 2212 16 -1.0412781098773536 2212 17 -1.2131929323390978 2212 18 -0.18624192164057263 2212 19 -0.07094494929184947 2212 20 -0.1379098217515491 2212 21 0.07457412519523388 2212 22 -0.30336690174124925 2212 23 0.04016659099311681 2212 24 -0.08566288010018042 2212 25 0.14702669060889662 2213 1 -3.0577627458311056 2213 2 -1.435988745522188 2213 3 -2.476098100400378 2213 4 -1.1118829306140015 2213 5 -0.8592016263182529 2213 6 -0.6433573812634137 2213 7 -0.41276475516938405 2213 8 -0.2171979480312629 2213 9 -0.45370573021972405 2213 10 -0.3512856832432121 2213 11 -0.59151532163893 2213 12 -0.30635235626308344 2213 13 -0.6440324692894492 2213 14 -0.38549846856520986 2213 15 -0.3927269501657378 2213 16 -1.6453741588361268 2213 17 -1.1094917298173002 2213 18 -0.4690889074407636 2213 19 -0.3273433570558453 2213 20 -0.34051644394383374 2213 21 -0.2066507122868515 2213 22 -0.15291804427994293 2213 23 -0.07337310063181235 2213 24 -0.0013496144757993989 2213 25 -0.17543761388180457 2214 1 -0.9235997225806685 2214 2 -0.6257421972557111 2214 3 -6.494906616602865 2214 4 -2.155777487306807 2214 5 -1.242520174076148 2214 6 -0.6232279520925772 2214 7 -0.45397110641686567 2214 8 -0.40768646440712786 2214 9 -0.11421101401607814 2214 10 -0.17610952057683704 2214 11 -0.7721935605468602 2214 12 -0.45808606130674434 2214 13 -5.133270147655505 2214 14 -3.803131025824399 2214 15 -2.1006230976323437 2214 16 -2.943836916524141 2214 17 -3.4006101622765996 2214 18 -1.1856358807804952 2214 19 -0.8320797740726462 2214 20 -0.4439190527550707 2214 21 -0.4725138768430881 2214 22 -0.409284137535991 2214 23 -0.3039244099499001 2214 24 -0.18788460283035135 2214 25 0.010265324642951157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1576.0 3 183.0 4 160.0 5 465.0 6 51.0 7 67.0 8 100.0 9 268.0 10 750.0 11 1960.0 12 4135.0 13 7695.0 14 12559.0 15 18749.0 16 23765.0 17 26927.0 18 28156.0 19 28296.0 20 28305.0 21 28193.0 22 28534.0 23 29535.0 24 30954.0 25 33397.0 26 36934.0 27 42000.0 28 48797.0 29 59769.0 30 74480.0 31 94127.0 32 123419.0 33 164923.0 34 223190.0 35 306630.0 36 435755.0 37 664507.0 38 1308107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.198776739655486 21.529146978287333 13.611582133121553 22.660494148935626 2 13.799668375191684 22.08415873447233 41.243792251258405 22.87238063907758 3 18.178544171690763 28.752823659585424 29.43798574352027 23.630646425203544 4 11.538165855706776 18.98706082441814 38.60921677135375 30.865556548521333 5 11.7150277580482 40.19994171793165 35.28951144966812 12.795519074352033 6 29.173762828331856 39.11228901505234 18.64348892309068 13.070459233525133 7 24.562535505993615 34.62263514756189 23.865085398300618 16.94974394814388 8 23.81271791466047 38.25619739715705 21.61601042158904 16.31507426659344 9 24.67900273657112 18.163470338997122 22.06811004423523 35.08941688019653 10 14.477531341784463 31.743266861519864 33.47336810446257 20.3058336922331 11 30.614780939303003 27.750991829349157 22.85378652501617 18.780440706331667 12 20.735858054486794 29.234243864635385 31.710075717113323 18.319822363764494 13 26.249354074141433 24.75903102053348 26.705276195725947 22.28633870959914 14 19.812662521776005 25.084549639067554 28.612108734411954 26.490679104744487 15 23.77572235376221 30.56754515054268 24.435843327406157 21.220889168288956 16 19.93477825526341 31.928156462710145 27.7198290042892 20.41723627773725 17 20.228849201939816 31.09360491774175 28.909281560639677 19.768264319678757 18 20.650878180132317 29.876965561247644 29.956803604803984 19.515352653816056 19 20.40555712375291 30.464502894920702 30.384265119302924 18.745674862023467 20 21.771836321303546 29.079393542306057 29.25196654290761 19.89680359348279 21 21.197855695467275 29.71440861379606 29.356190112260016 19.73154557847665 22 20.618032907907363 29.36907991150377 28.59191522917341 21.420971951415456 23 19.90671699933125 29.953401925127356 29.27019169020582 20.86968938533558 24 20.400979276844467 30.449961002136515 28.642519244098015 20.506540476921007 25 20.823545279816567 28.789726554397394 27.997520075940653 22.38920808984539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5003.0 1 5003.0 2 5355.5 3 5708.0 4 5708.0 5 5708.0 6 8788.5 7 11869.0 8 11869.0 9 11869.0 10 17093.0 11 22317.0 12 22317.0 13 22317.0 14 34323.5 15 46330.0 16 46330.0 17 46330.0 18 71271.5 19 96213.0 20 96213.0 21 96213.0 22 132453.5 23 168694.0 24 168694.0 25 168694.0 26 216335.5 27 263977.0 28 263977.0 29 263977.0 30 307507.5 31 351038.0 32 351038.0 33 351038.0 34 382811.0 35 414584.0 36 414584.0 37 414584.0 38 426018.5 39 437453.0 40 437453.0 41 437453.0 42 440115.5 43 442778.0 44 442778.0 45 442778.0 46 498001.5 47 553225.0 48 553225.0 49 553225.0 50 493572.0 51 433919.0 52 433919.0 53 433919.0 54 356160.5 55 278402.0 56 278402.0 57 278402.0 58 227960.5 59 177519.0 60 177519.0 61 177519.0 62 145496.5 63 113474.0 64 113474.0 65 113474.0 66 84665.5 67 55857.0 68 55857.0 69 55857.0 70 40271.5 71 24686.0 72 24686.0 73 24686.0 74 17105.5 75 9525.0 76 9525.0 77 9525.0 78 6405.5 79 3286.0 80 3286.0 81 3286.0 82 2178.0 83 1070.0 84 1070.0 85 1070.0 86 686.5 87 303.0 88 303.0 89 303.0 90 212.5 91 122.0 92 122.0 93 122.0 94 78.5 95 35.0 96 35.0 97 35.0 98 33.0 99 31.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10062750515773398 2 0.054883088810027424 3 0.05835476326498729 4 0.05358121088941747 5 0.05046691468717405 6 0.07002061051437451 7 0.06601286868033995 8 0.06300068055030124 9 0.06802950310638282 10 0.06831030030494577 11 0.06203065386435658 12 0.06256672124343127 13 0.06368991003768298 14 0.06529811217490704 15 0.06843793539520164 16 0.06294962651419889 17 0.0667020981677217 18 0.14675482677620819 19 0.07155223159744505 20 0.06517047708465117 21 0.06353674792937593 22 0.06828477328689458 23 0.06800397608833166 24 0.07915928297669536 25 0.07101616421837036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3917418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.98510399712636 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9740013040526 57.08981211710841 2 11.304439719128784 15.370670198684325 3 2.6576253200319 5.42036801303295 4 0.8893951589292767 2.418624894973904 5 0.3580650276502245 1.217154407126722 6 0.19560501713889847 0.7978936459528617 7 0.12849169075953268 0.6114864671337401 8 0.08029459992008349 0.43670693807796246 9 0.0620442864520222 0.37962785401814053 >10 0.30434403481268707 3.759843181699305 >50 0.023969896407292954 1.1417638482245158 >100 0.016867500513548576 2.293924109918465 >500 0.002086753390419563 0.9777810546150255 >1k 0.002238517232290633 3.226236352104971 >5k 2.6558679027176947E-4 1.3405249865593334 >10k+ 2.6558679027176947E-4 3.517581930769345 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 37670 0.9616027699877828 No Hit CCCATGTACTCTGCGTTGATACCAC 36464 0.930817186218065 No Hit GAGTACATGGGAAGCAGTGGTATCA 16411 0.4189238932378419 No Hit CATGTACTCTGCGTTGATACCACTG 12974 0.33118753219595154 No Hit GTATCAACGCAGAGTACTTTTTTTT 11233 0.28674499376885487 No Hit GCGTTGATACCACTGCTTCCCATGT 11212 0.2862089263897802 No Hit GTATCAACGCAGAGTACATGGGAAG 10407 0.2656596768585839 No Hit ACGCAGAGTACATGGGAAGCAGTGG 8873 0.2265012311680806 No Hit TATCAACGCAGAGTACATGGGAAGC 8456 0.2158564646407404 No Hit ACTCTGCGTTGATACCACTGCTTCC 7548 0.192677932250273 No Hit GGTATCAACGCAGAGTACATGGGAA 7063 0.18029732849545288 No Hit TATCAACGCAGAGTACTTTTTTTTT 6689 0.17075022374431323 No Hit GGTATCAACGCAGAGTACTTTTTTT 6688 0.17072469672626203 No Hit GCTTCCCATGTACTCTGCGTTGATA 6653 0.16983125109447092 No Hit GTGGTATCAACGCAGAGTACATGGG 4921 0.12561845582983486 No Hit CAGTGGTATCAACGCAGAGTACATG 4530 0.11563739177182521 No Hit ATACCACTGCTTCCCATGTACTCTG 4236 0.10813244846477961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.658105415352664E-5 0.0 5 0.0 0.0 0.0 7.658105415352664E-5 0.0 6 0.0 0.0 0.0 7.658105415352664E-5 0.0 7 0.0 0.0 0.0 7.658105415352664E-5 0.0 8 0.0 0.0 0.0 1.0210807220470218E-4 0.0 9 0.0 2.5527018051175546E-5 0.0 1.0210807220470218E-4 0.0 10 0.0 2.5527018051175546E-5 0.0 1.0210807220470218E-4 0.0 11 0.0 5.105403610235109E-5 0.0 1.2763509025587772E-4 0.0 12 0.0 5.105403610235109E-5 0.0 1.2763509025587772E-4 0.0 13 0.0 5.105403610235109E-5 0.0 1.2763509025587772E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCGC 55 1.9671406E-4 13.813379 10 TGGACCG 70 1.09500426E-4 12.210352 5 GTCCGGA 55 0.0030719957 12.089795 1 TACGCTG 65 8.0499204E-4 11.688542 5 CAGTGCG 60 0.005893591 11.079481 9 TAGACTG 520 0.0 10.958008 5 TACCGTA 80 3.7846787E-4 10.684058 2 TGTCCCG 455 0.0 10.644243 11 TAGGACG 90 9.553772E-5 10.552156 4 GGACCGT 100 2.4098154E-5 10.446502 6 GTCCCGC 485 0.0 10.386856 12 TCCCGCC 505 0.0 10.351931 13 CGCCCTA 510 0.0 9.505198 16 CACACCG 110 6.865966E-5 9.496941 5 TATACCG 80 0.004530055 9.496941 5 AGTGCGA 80 0.0045310096 9.496698 10 CCCGCCC 525 0.0 9.41431 14 CTACACT 520 0.0 9.314307 4 GTCTTAC 380 0.0 9.249147 1 TAGGACC 250 1.8189894E-12 9.117063 4 >>END_MODULE