FastQCFastQC Report
Wed 25 May 2016
SRR1294623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917418
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC405351.0347376767044008No Hit
GTACATGGGAAGCAGTGGTATCAAC366230.934875982088202No Hit
GTATCAACGCAGAGTACTTTTTTTT264300.6746790870925696No Hit
GGTATCAACGCAGAGTACTTTTTTT178870.4566017718813769No Hit
TATCAACGCAGAGTACTTTTTTTTT175780.4487139233035637No Hit
GAGTACATGGGAAGCAGTGGTATCA171730.43837548099283763No Hit
CATGTACTCTGCGTTGATACCACTG145480.37136705860850183No Hit
GCGTTGATACCACTGCTTCCCATGT119250.3044096902602684No Hit
ACGCAGAGTACATGGGAAGCAGTGG107460.2743133359779324No Hit
GTATCAACGCAGAGTACATGGGAAG103320.26374515050474573No Hit
ACGCAGAGTACTTTTTTTTTTTTTT101400.25884396303892No Hit
TATCAACGCAGAGTACATGGGAAGC91160.23270429655451624No Hit
ACTCTGCGTTGATACCACTGCTTCC85380.2179496801209368No Hit
GGTATCAACGCAGAGTACATGGGAA76530.19535826914564644No Hit
GCTTCCCATGTACTCTGCGTTGATA71040.18134393623555106No Hit
GTACTTTTTTTTTTTTTTTTTTTTT59720.15244735180162033No Hit
CAGTGGTATCAACGCAGAGTACATG55330.1412409908771543No Hit
GAGTACTTTTTTTTTTTTTTTTTTT53350.13618664130302152No Hit
GTGGTATCAACGCAGAGTACATGGG51320.1310046566386329No Hit
CATGGGAAGCAGTGGTATCAACGCA44520.11364628436383352No Hit
ATACCACTGCTTCCCATGTACTCTG43400.11078725834210187No Hit
GGGAAGCAGTGGTATCAACGCAGAG41790.1066774084358626No Hit
GCAGTGGTATCAACGCAGAGTACAT41390.10565632771381558No Hit
ACATGGGAAGCAGTGGTATCAACGC40830.10422681470294975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC400.00521388814.2774967
GACGGAC550.003025872612.114247
GCTCCGC752.0385215E-411.4219967
AGTACCG803.706807E-410.7081225
CGCCTTT3100.010.70795914
CGCAGCG1752.2737368E-1010.3341888
GTCGCCC1303.9125734E-710.21804911
CCGTACA5050.09.95370617
GAACGGT1054.1838157E-59.93594619
ACCGTCT1151.004144E-59.9321728
TGTCCCG4750.09.78781511
CTGGTCG900.00109573179.5174779
ATCGCCG1201.7355476E-59.48431219
GTCCCGC4850.09.38653712
CGCGCCT3150.09.33376812
CGCCCTA4900.09.29063716
ACACGGT6050.09.2823396
CTACACT5950.09.2783734
GTCTAGG3200.09.2119151
TAGACAG5200.09.1522415
Produced by FastQC (version 0.11.5)