##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294623_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3917418 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.228969183273268 31.0 31.0 34.0 28.0 34.0 2 31.448364458426443 31.0 31.0 34.0 28.0 34.0 3 31.577718538077885 31.0 31.0 34.0 28.0 34.0 4 35.16509241546345 37.0 35.0 37.0 32.0 37.0 5 35.299260124908805 37.0 35.0 37.0 32.0 37.0 6 34.845728742758624 37.0 35.0 37.0 32.0 37.0 7 34.70446120378269 37.0 35.0 37.0 32.0 37.0 8 34.734390611366976 37.0 35.0 37.0 32.0 37.0 9 36.2060591951127 39.0 37.0 39.0 31.0 39.0 10 36.02510556698315 39.0 35.0 39.0 30.0 39.0 11 36.15600837081976 39.0 35.0 39.0 31.0 39.0 12 36.122006893315955 39.0 35.0 39.0 30.0 39.0 13 35.97316753024569 39.0 35.0 39.0 30.0 39.0 14 37.0602657158363 40.0 36.0 41.0 30.0 41.0 15 37.08020384855535 40.0 36.0 41.0 31.0 41.0 16 37.10984862988836 40.0 36.0 41.0 31.0 41.0 17 37.180897213419655 40.0 37.0 41.0 31.0 41.0 18 37.192313661702684 40.0 37.0 41.0 31.0 41.0 19 37.201878635366455 40.0 37.0 41.0 30.0 41.0 20 37.271495153185086 40.0 37.0 41.0 31.0 41.0 21 37.11562054393991 40.0 37.0 41.0 30.0 41.0 22 37.14343503807865 40.0 37.0 41.0 30.0 41.0 23 37.151661374915825 40.0 37.0 41.0 30.0 41.0 24 37.068967110479406 40.0 36.0 41.0 30.0 41.0 25 36.019819687355294 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4132748520544034 1101 2 0.4788185163006524 1101 3 0.3882976750339786 1101 4 0.3450699820267147 1101 5 0.41774250506571775 1101 6 0.4629032981827095 1101 7 0.5122149279449602 1101 8 0.5246949768265807 1101 9 0.7015347191676327 1101 10 0.738701306804316 1101 11 0.7295266902658568 1101 12 0.7812908170308077 1101 13 0.7501490188269813 1101 14 0.937604464053841 1101 15 0.8817540094629948 1101 16 0.9217729870006153 1101 17 0.9110500562026189 1101 18 0.902558294107223 1101 19 1.0346620556584867 1101 20 0.9696680221872995 1101 21 1.1649478258290316 1101 22 1.0566128110775992 1101 23 1.0937211639263253 1101 24 1.2280559862835219 1101 25 1.1498490614698937 1102 1 0.41600285630499556 1102 2 0.40267448690401864 1102 3 0.33749372846699544 1102 4 0.28816009104532014 1102 5 0.3738926696426361 1102 6 0.4699584290563976 1102 7 0.5341004806073997 1102 8 0.5034967573274329 1102 9 0.6932595710222884 1102 10 0.8592521947768077 1102 11 0.7830818738076388 1102 12 0.7851202627912244 1102 13 0.7900766770931611 1102 14 0.9931336855613395 1102 15 0.9459758608321707 1102 16 0.8851304105469993 1102 17 0.8860673147977494 1102 18 0.9190402887278211 1102 19 1.0171845179485643 1102 20 0.9955662344899281 1102 21 1.2200741654195681 1102 22 1.1018381833742978 1102 23 0.9889830738078729 1102 24 1.1341551186673229 1102 25 1.1485749070540052 1103 1 0.2887803966530562 1103 2 0.28888909063057966 1103 3 0.24566612415877032 1103 4 0.2141731015455477 1103 5 0.1966046291476644 1103 6 0.17354760465990182 1103 7 0.2535685991446357 1103 8 0.31704279527372137 1103 9 0.20222833677834728 1103 10 0.45911715966395406 1103 11 0.4510094300302683 1103 12 0.4526241730884948 1103 13 0.4993003110903089 1103 14 0.6397857563779539 1103 15 0.6552473049088832 1103 16 0.5947937352080714 1103 17 0.5665992286741286 1103 18 0.5432874217842425 1103 19 0.7144348873422075 1103 20 0.6171771683796052 1103 21 0.8943949827223392 1103 22 0.7548932393550416 1103 23 0.7620728706993134 1103 24 0.914770060014078 1103 25 0.8377935072170075 1104 1 0.19777090297841937 1104 2 0.1871499210446359 1104 3 0.12176842276928213 1104 4 0.0889578604954977 1104 5 0.11659172303237852 1104 6 0.16965641772419815 1104 7 0.1780633905796023 1104 8 0.07793004563961148 1104 9 -0.03643677753534291 1104 10 0.29232380511053435 1104 11 0.2933683013939685 1104 12 0.36198481556932194 1104 13 -0.13534893185397578 1104 14 0.22760726436557377 1104 15 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0.12146721997907406 2207 1 0.21897937219014096 2207 2 0.26023500980452496 2207 3 0.24866920543285076 2207 4 0.18267913326428697 2207 5 0.13389323802603315 2207 6 0.3300202972837383 2207 7 0.30426319410641867 2207 8 0.268830707529915 2207 9 0.36248090715720593 2207 10 0.5152841839648943 2207 11 0.41927463400882203 2207 12 0.38719102204245814 2207 13 0.39877124201349545 2207 14 0.41976924189511067 2207 15 0.40992700960326545 2207 16 0.5194345182782669 2207 17 0.510476203702936 2207 18 0.5011676153691056 2207 19 0.642196015489084 2207 20 0.5226437954976859 2207 21 0.8463460887974108 2207 22 0.723788151985886 2207 23 0.6401625661274153 2207 24 0.7853089420591957 2207 25 0.7216904028661446 2208 1 0.21091200980024993 2208 2 0.22281878437204128 2208 3 0.17194131553062775 2208 4 0.1631586166715593 2208 5 0.13215561942323006 2208 6 0.2743523983999978 2208 7 0.30843435854389867 2208 8 0.19542893667689754 2208 9 0.35144533479466844 2208 10 0.4365546107297149 2208 11 0.07280490913851878 2208 12 -0.08817244629157983 2208 13 0.11647960642639532 2208 14 -0.34400834496382515 2208 15 0.2323799452609805 2208 16 0.34848856216630963 2208 17 0.23585831977564453 2208 18 0.3608661098048742 2208 19 0.5083741687802572 2208 20 0.5234211033736997 2208 21 0.6615557964850183 2208 22 0.7042756045122474 2208 23 0.5767321395972118 2208 24 0.7101622210138103 2208 25 0.7679064483622966 2209 1 0.08929450724447108 2209 2 0.09314109515394975 2209 3 0.136020829950116 2209 4 -0.4679913497687096 2209 5 -0.06888362535821813 2209 6 0.11090755466518942 2209 7 0.1284044830074862 2209 8 0.18197883889825306 2209 9 0.2857499411026794 2209 10 0.31404665587835723 2209 11 0.32257216644622133 2209 12 0.23873028087520964 2209 13 0.3509347608087765 2209 14 0.35282416629611646 2209 15 -0.18443467426227755 2209 16 -0.19496530631111142 2209 17 0.17931246812948132 2209 18 -0.08153163225389193 2209 19 0.28326192561140573 2209 20 0.4096019121962158 2209 21 0.6072934820494496 2209 22 0.6496893967207953 2209 23 0.5424951107246514 2209 24 0.676565536684393 2209 25 0.7969480802352322 2210 1 -0.1275386928481339 2210 2 -0.14888119963481827 2210 3 -0.08126647273295262 2210 4 -0.056480918110075606 2210 5 -0.03279614797115471 2210 6 -0.5094495654605993 2210 7 -0.2289503003346951 2210 8 -0.35756118205783594 2210 9 -0.0828721405645112 2210 10 0.1480285959256875 2210 11 0.055198732581146714 2210 12 0.07914495679948175 2210 13 0.08127266069707417 2210 14 0.17042320332584637 2210 15 0.00543754562110621 2210 16 0.13108129151038383 2210 17 -0.9701864755141045 2210 18 -1.571497295504365 2210 19 -0.3920094820344602 2210 20 -0.1722175762776459 2210 21 -0.37460692052130895 2210 22 -0.2556579915224688 2210 23 -0.17940530647047837 2210 24 0.23218130119592217 2210 25 0.2189238065784238 2211 1 -0.21426853092819087 2211 2 -0.2186196997546439 2211 3 -0.18028925036812282 2211 4 -0.1088057313320121 2211 5 -0.07321632578172199 2211 6 -0.014044853677802394 2211 7 -0.20344277644358044 2211 8 -0.13741274909165924 2211 9 -0.10412506604399141 2211 10 -0.05783905516317844 2211 11 -0.32234602193595663 2211 12 -0.17274736910864164 2211 13 -0.08039388884504461 2211 14 -0.17462918987720855 2211 15 -0.1644634070476414 2211 16 -0.21142428088510457 2211 17 -0.3838828645758099 2211 18 -0.38055172278794913 2211 19 -0.1938408917813348 2211 20 -0.32608606652648575 2211 21 0.042055936053081666 2211 22 -0.10169051949654317 2211 23 -0.5535882168212964 2211 24 -0.10110897434155675 2211 25 -0.6561306154674966 2212 1 -0.10407636122264208 2212 2 -0.2856436771212856 2212 3 -0.18737256462449992 2212 4 -0.03288157694489513 2212 5 -0.094105284144689 2212 6 -0.17140831981389226 2212 7 -0.14938353041034702 2212 8 -0.3088981996557152 2212 9 -0.09667501087869823 2212 10 -0.5006939939493691 2212 11 -0.17550143287833464 2212 12 -0.2591335840703195 2212 13 -0.21868251479582312 2212 14 -0.1935340439225115 2212 15 -0.11449298887763604 2212 16 -0.34845260699551517 2212 17 -0.4658641608607397 2212 18 -0.34734015938899176 2212 19 -0.3626985790391686 2212 20 -0.4928465332965146 2212 21 0.012100806655361396 2212 22 -0.3098767226493706 2212 23 -0.16000175930946625 2212 24 -0.37993293758249536 2212 25 -0.14769045074027076 2213 1 -0.2726596284975322 2213 2 -0.26693299332469067 2213 3 -0.15774354729099116 2213 4 -0.04915624472959479 2213 5 -0.12327664527783355 2213 6 -0.25562159122052464 2213 7 -0.35238001186381496 2213 8 -0.3440865122799721 2213 9 -0.3777576287910662 2213 10 -0.35522045766800403 2213 11 -0.11094237953276576 2213 12 0.022124811643152498 2213 13 0.03693005015811224 2213 14 -0.6937547600275877 2213 15 -0.35823242235488095 2213 16 -0.33146576692578833 2213 17 -0.3234834793997905 2213 18 -0.36896073938804363 2213 19 -0.39482014564843126 2213 20 -0.5005188510989171 2213 21 -0.07805889739756822 2213 22 -0.5113960330746323 2213 23 -0.5855672831082401 2213 24 -0.6214872359558896 2213 25 -0.5648757105682805 2214 1 -0.24436729744320118 2214 2 -0.16581067423366846 2214 3 -0.15349028627694494 2214 4 -0.17488787786623305 2214 5 -0.16779721697390926 2214 6 -0.1536205538221722 2214 7 -0.22477622145873966 2214 8 -0.1318214801177291 2214 9 -0.2575747042524057 2214 10 -0.21981768558933368 2214 11 -0.11644124099529307 2214 12 -0.3438688223719595 2214 13 -0.31329707939820395 2214 14 -0.27051895129032744 2214 15 -0.20829317806635572 2214 16 -0.5178152372457916 2214 17 -0.43206549455332066 2214 18 -0.33885639271782964 2214 19 -0.5699105567933884 2214 20 -0.5952523928751461 2214 21 -0.5226789466378676 2214 22 -0.7456092285505349 2214 23 -1.043828723387037 2214 24 -0.5876062824741055 2214 25 -0.8066679823839848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 43.0 10 103.0 11 239.0 12 637.0 13 1546.0 14 3421.0 15 6308.0 16 9007.0 17 12107.0 18 14137.0 19 15897.0 20 17182.0 21 18444.0 22 19796.0 23 21482.0 24 24169.0 25 27124.0 26 30775.0 27 34861.0 28 40953.0 29 51213.0 30 65669.0 31 85368.0 32 115249.0 33 156067.0 34 215856.0 35 300601.0 36 433055.0 37 689343.0 38 1506754.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.969379111996545 21.725165003662774 12.83000413163908 24.4754517527016 2 13.67160716574029 22.43679382695439 39.72256726241621 24.169031744889107 3 17.842523790900795 28.180465772542885 30.068861334130837 23.908149102425483 4 11.537289114394063 18.94297938132223 38.418237325418296 31.10149417886541 5 11.899407862501338 39.530839994699335 35.503355173472976 13.066396969326352 6 29.578907083930854 39.10608420632855 18.046179113523802 13.268829596216783 7 24.546073596934445 34.42058301836642 23.82977973721253 17.2035636474866 8 24.241662919822122 37.86386200990699 21.401822843062234 16.492652227208655 9 24.80620511229982 17.007261165845332 22.36819433223184 35.818339389623006 10 14.452399866745253 31.16500525331215 33.793096379058504 20.589498500884094 11 31.008520434124243 26.743347849098708 22.887465694498655 19.360666022278398 12 20.95365367703463 28.75639302539596 31.983315272677608 18.306638024891793 13 26.853606125259034 23.748831500748707 27.061574741321966 22.335987632670296 14 20.068305752866834 24.221023917479666 29.156100522133894 26.554569807519606 15 24.018628796418827 29.709198197977422 24.9196911401918 21.35248186541195 16 19.893608385531884 31.200353090074216 28.242738704665882 20.663299819728014 17 20.033435305313503 30.52011836373773 29.548891412094097 19.897554918854674 18 20.4368145655569 29.051341627597445 30.86861560941092 19.64322819743473 19 20.31539294312374 29.66721030969784 31.423503196891613 18.59389355028681 20 21.43591228086298 28.186083056875304 30.606303345167678 19.77170131709404 21 20.822410935391225 29.16159635105755 30.29780653488058 19.718186178670646 22 20.287432890587564 28.791408974096168 29.680950921004968 21.240207214311297 23 19.62375699281286 29.089973873077017 30.485868870160555 20.80040026394957 24 20.115320886185977 29.797695777600392 29.853446799255735 20.2335365369579 25 20.592652308110175 28.060502295052842 29.11241534112825 22.234430055708735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3461.0 1 3461.0 2 3870.5 3 4280.0 4 4280.0 5 4280.0 6 8441.0 7 12602.0 8 12602.0 9 12602.0 10 16835.0 11 21068.0 12 21068.0 13 21068.0 14 30308.5 15 39549.0 16 39549.0 17 39549.0 18 63225.0 19 86901.0 20 86901.0 21 86901.0 22 125770.0 23 164639.0 24 164639.0 25 164639.0 26 214255.5 27 263872.0 28 263872.0 29 263872.0 30 309313.5 31 354755.0 32 354755.0 33 354755.0 34 385928.5 35 417102.0 36 417102.0 37 417102.0 38 425717.0 39 434332.0 40 434332.0 41 434332.0 42 437627.0 43 440922.0 44 440922.0 45 440922.0 46 499813.0 47 558704.0 48 558704.0 49 558704.0 50 501189.5 51 443675.0 52 443675.0 53 443675.0 54 362926.5 55 282178.0 56 282178.0 57 282178.0 58 230427.0 59 178676.0 60 178676.0 61 178676.0 62 147011.0 63 115346.0 64 115346.0 65 115346.0 66 85837.5 67 56329.0 68 56329.0 69 56329.0 70 40607.0 71 24885.0 72 24885.0 73 24885.0 74 17211.5 75 9538.0 76 9538.0 77 9538.0 78 6327.0 79 3116.0 80 3116.0 81 3116.0 82 2059.0 83 1002.0 84 1002.0 85 1002.0 86 645.5 87 289.0 88 289.0 89 289.0 90 224.0 91 159.0 92 159.0 93 159.0 94 94.5 95 30.0 96 30.0 97 30.0 98 19.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0948839260962195 2 0.0 3 0.13294471001052224 4 0.012355076736768965 5 0.023714599769542082 6 0.01375906272958362 7 0.02218297868647155 8 0.020140817242377505 9 7.658105415352664E-5 10 0.3859174588976719 11 0.06621708482474936 12 0.0016082021372240594 13 0.0 14 8.168645776376175E-4 15 0.001072134758149373 16 1.0210807220470218E-4 17 5.105403610235109E-5 18 3.0632421661410655E-4 19 4.850133429723354E-4 20 1.5316210830705328E-4 21 2.297431624605799E-4 22 0.003088769184192241 23 7.658105415352664E-5 24 2.5527018051175546E-5 25 0.002016634426042868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3917418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.56566987314851 #Duplication Level Percentage of deduplicated Percentage of total 1 83.07958882148839 54.47168893866626 2 11.95351464183448 15.674803896607333 3 2.8212898650918987 5.549392797332254 4 0.94907824241279 2.4890780290330006 5 0.3844470937962862 1.2603265617769333 6 0.20245150113872606 0.7964320973391037 7 0.11930848065474353 0.5475778318973088 8 0.08089998407816101 0.4243409319049341 9 0.05360600438262879 0.3163242227912992 >10 0.30681428056246307 3.6982294414376113 >50 0.025501138334673427 1.1767629507581592 >100 0.018167640711420758 2.4038797724702055 >500 0.0024701121355282293 1.1604172480363344 >1k 0.002117238937020179 3.133537843109174 >5k 3.1366502770669314E-4 1.3980277004758743 >10k+ 4.31289413096703E-4 5.499179736364261 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 40535 1.0347376767044008 No Hit GTACATGGGAAGCAGTGGTATCAAC 36623 0.934875982088202 No Hit GTATCAACGCAGAGTACTTTTTTTT 26430 0.6746790870925696 No Hit GGTATCAACGCAGAGTACTTTTTTT 17887 0.4566017718813769 No Hit TATCAACGCAGAGTACTTTTTTTTT 17578 0.4487139233035637 No Hit GAGTACATGGGAAGCAGTGGTATCA 17173 0.43837548099283763 No Hit CATGTACTCTGCGTTGATACCACTG 14548 0.37136705860850183 No Hit GCGTTGATACCACTGCTTCCCATGT 11925 0.3044096902602684 No Hit ACGCAGAGTACATGGGAAGCAGTGG 10746 0.2743133359779324 No Hit GTATCAACGCAGAGTACATGGGAAG 10332 0.26374515050474573 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10140 0.25884396303892 No Hit TATCAACGCAGAGTACATGGGAAGC 9116 0.23270429655451624 No Hit ACTCTGCGTTGATACCACTGCTTCC 8538 0.2179496801209368 No Hit GGTATCAACGCAGAGTACATGGGAA 7653 0.19535826914564644 No Hit GCTTCCCATGTACTCTGCGTTGATA 7104 0.18134393623555106 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5972 0.15244735180162033 No Hit CAGTGGTATCAACGCAGAGTACATG 5533 0.1412409908771543 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5335 0.13618664130302152 No Hit GTGGTATCAACGCAGAGTACATGGG 5132 0.1310046566386329 No Hit CATGGGAAGCAGTGGTATCAACGCA 4452 0.11364628436383352 No Hit ATACCACTGCTTCCCATGTACTCTG 4340 0.11078725834210187 No Hit GGGAAGCAGTGGTATCAACGCAGAG 4179 0.1066774084358626 No Hit GCAGTGGTATCAACGCAGAGTACAT 4139 0.10565632771381558 No Hit ACATGGGAAGCAGTGGTATCAACGC 4083 0.10422681470294975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.658105415352664E-5 0.0 5 0.0 0.0 0.0 7.658105415352664E-5 0.0 6 0.0 0.0 0.0 7.658105415352664E-5 0.0 7 0.0 0.0 0.0 1.0210807220470218E-4 0.0 8 0.0 0.0 0.0 1.5316210830705328E-4 0.0 9 0.0 0.0 0.0 1.786891263582288E-4 0.0 10 0.0 0.0 0.0 1.786891263582288E-4 0.0 11 0.0 0.0 0.0 2.0421614440940437E-4 0.0 12 0.0 0.0 0.0 2.0421614440940437E-4 0.0 13 0.0 0.0 0.0 2.0421614440940437E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGTC 40 0.005213888 14.277496 7 GACGGAC 55 0.0030258726 12.11424 7 GCTCCGC 75 2.0385215E-4 11.421996 7 AGTACCG 80 3.706807E-4 10.708122 5 CGCCTTT 310 0.0 10.707959 14 CGCAGCG 175 2.2737368E-10 10.334188 8 GTCGCCC 130 3.9125734E-7 10.218049 11 CCGTACA 505 0.0 9.953706 17 GAACGGT 105 4.1838157E-5 9.935946 19 ACCGTCT 115 1.004144E-5 9.932172 8 TGTCCCG 475 0.0 9.787815 11 CTGGTCG 90 0.0010957317 9.517477 9 ATCGCCG 120 1.7355476E-5 9.484312 19 GTCCCGC 485 0.0 9.386537 12 CGCGCCT 315 0.0 9.333768 12 CGCCCTA 490 0.0 9.290637 16 ACACGGT 605 0.0 9.282339 6 CTACACT 595 0.0 9.278373 4 GTCTAGG 320 0.0 9.211915 1 TAGACAG 520 0.0 9.152241 5 >>END_MODULE