FastQCFastQC Report
Wed 25 May 2016
SRR1294622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231289
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51342.219733753010303No Hit
GGTATCAACGCAGAGTACTTTTTTT36261.56773560350903No Hit
TATCAACGCAGAGTACTTTTTTTTT32941.4241922443350095No Hit
GTACTGGTTCACTATCGGTCAGTCA23120.9996152000311299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17090.7389024121337374No Hit
ATACAGGGTGACAGCCCCGTACACA16020.6926399439662068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14000.6053033218181583No Hit
GGATACCACGTGTCCCGCCCTACTC13390.5789293913675099No Hit
GTTAATGATAGTGTGTCGAAACACA11270.4872691740636174No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10810.4673806363467351No Hit
ACTTAGATGTTTCAGTTCCCCCGGT9030.39042064257271203No Hit
GTTCACTATCGGTCAGTCAGGAGTA8840.3822058117766085No Hit
GGTATCAACGCAGAGTACATGGGGG8650.3739909809805049No Hit
GTATTTAGCCTTGGAGGATGGTCCC8400.36318199309089494No Hit
CCCATATTCAGACAGGATACCACGT7150.3091370536428451No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6960.3009222228467415No Hit
GTACTTAGATGTTTCAGTTCCCCCG6830.29530154914414436No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6800.29400447059739115No Hit
AGCGTACACGGTGGATGCCCTGGCA6770.29270739205063795No Hit
GCCTTGGAGGATGGTCCCCCCATAT6750.29184267301946915No Hit
CTATCGGTCAGTCAGGAGTATTTAG6500.28103368512985916No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG6480.28016896609869035No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6410.2771424494895996No Hit
GTACATGGGGGTTAAGCGACTAAGC6290.2719541353025868No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6230.2693599782090804No Hit
ATCATTAACTGAATCCATAGGTTAA6140.26546874256882086No Hit
TATTCAGACAGGATACCACGTGTCC6070.26244222595973005No Hit
CGGTACTGGTTCACTATCGGTCAGT6010.25984806886622364No Hit
TCATTAACCTATGGATTCAGTTAAT5970.25811863080388603No Hit
GTGGATGCCCTGGCAGTCAGAGGCG5880.25422739516362647No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5840.25249795710128886No Hit
CACACACACTGATTCAGGCTCTGGG5820.25163323807012006No Hit
TCACGGTACTGGTTCACTATCGGTC5700.24644492388310726No Hit
GGTTAATGAGGCGAACCGGGGGAAC5560.24039189066492572No Hit
CATCTAAGTACCCCGAGGAAAAGAA5410.23390649793115972No Hit
GTATCAACGCAGAGTACATGGGGGT5370.23217705986882214No Hit
GGGTTTCCCCATTCGGAAATCGCCG5340.23087998132206894No Hit
GGTTCACTATCGGTCAGTCAGGAGT5260.22742110519739372No Hit
CCTATGGATTCAGTTAATGATAGTG5120.22136807197921218No Hit
GTGCTAATCTGCGATAAGCGTCGGT5080.21963863391687455No Hit
ACGCAGAGTACATGGGGGTTAAGCG5070.21920627440129015No Hit
GGGTACTTAGATGTTTCAGTTCCCC4960.21445031972986178No Hit
ATCAACGCAGAGTACTTTTTTTTTT4830.2088296460272646No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4610.19931773668440783No Hit
GGGTGACAGCCCCGTACACAAAAAT4300.18591459170129146No Hit
CCCCAGTAGCGGCGAGCGAACGGGG4270.18461751315453828No Hit
ATGATAGTGTGTCGAAACACACTGG4250.18375279412336945No Hit
GGTATCAACGCAGAGTACATGGGGA4180.18072627751427867No Hit
GTTATAACCGGCGATTTCCGAATGG4140.17899683945194106No Hit
GTCCCGCCCTACTCATCGAGCTCAC4120.17813212042077228No Hit
AGCTCACAGCATGTGCATTTTTGTG4110.17769976090518788No Hit
TAATGATAGTGTGTCGAAACACACT4060.1755379633272659No Hit
TTCCAGACGCTTCCACTAACACACA3970.1716467276870063No Hit
GCCCTACTCATCGAGCTCACAGCAT3890.16818785156233113No Hit
GTGGTATCAACGCAGAGTACATGGG3860.16689077301557792No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3800.1642966159220715No Hit
GGAGTATTTAGCCTTGGAGGATGGT3720.16083773979739632No Hit
CCTATGTATTCAGTATGCAATGACC3680.15910830173505874No Hit
GTGGTATCAACGCAGAGTACTTTTT3600.15564942561038356No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC3580.15478470657921475No Hit
AAACAAAAAAAAAAAAAAAAAAAAA3460.14959639239220196No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3460.14959639239220196No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3410.14743459481427998No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3400.14700223529869558No Hit
GTCCCCCCATATTCAGACAGGATAC3300.1426786401428516No Hit
CATTAACCTATGGATTCAGTTAATG3280.14181392111168278No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA3250.14051684256492958No Hit
CACTATCATTAACTGAATCCATAGG3220.1392197640181764No Hit
TATCAACGCAGAGTACATGGGGGTT3190.1379226854714232No Hit
GATATGAACCGTTATAACCGGCGAT3190.1379226854714232No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA3140.1357608878935012No Hit
GGTGATATGAACCGTTATAACCGGC3030.13100493322207282No Hit
CTACTGGGGGAATCTCGGTTGATTT3020.13057257370648842No Hit
CTGTCACCCTGTATCGCGCGCCTTT2940.12711369758181323No Hit
TCGGTAAGGTGATATGAACCGTTAT2890.12495190000389124No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2840.12279010242596923No Hit
GAGTACATGGGGGTTAAGCGACTAA2830.12235774291038484No Hit
GTATCGCGCGCCTTTCCAGACGCTT2820.12192538339480044No Hit
TTTGTGTACGGGGCTGTCACCCTGT2770.11976358581687846No Hit
CCGTTATAACCGGCGATTTCCGAAT2590.11198111453635928No Hit
ACTTAGATGTTTCAGTTCGCCGCGT2580.11154875502077488No Hit
GTTCATATCACCTTACCGACGCTTA2530.10938695744285287No Hit
TAAGTACCCCGAGGAAAAGAAATCA2530.10938695744285287No Hit
GGGCTGTCACCCTGTATCGCGCGCC2480.10722515986493089No Hit
AACACACACACTGATTCAGGCTCTG2460.1063604408337621No Hit
GTTATAACGGTTCATATCACCTTAC2430.10506336228700888No Hit
CTATCATTAACTGAATCCATAGGTT2420.1046310027714245No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2410.10419864325584009No Hit
GTACGGGGCTGTCACCCTGTATCGC2400.1037662837402557No Hit
CAGTTAATGATAGTGTGTCGAAACA2390.1033339242246713No Hit
GTCGAAACACACTGGGTTTCCCCAT2380.1029015647090869No Hit
GTACTGGTTCACTATCGGTCGATTA2380.1029015647090869No Hit
CTAACACACACACTGATTCAGGCTC2370.10246920519350251No Hit
GTATCAACGCAGAGTACATGGGGGG2340.1011721266467493No Hit
GGTTATAACGGTTCATATCACCTTA2320.1003074076155805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAGGT250.00600381119.00714916
GTACTAG307.6984987E-418.9989171
GTACAAA359.897414E-518.9989171
ATACCTA307.7097246E-418.9948045
TACCTAT307.7097246E-418.9948046
CATACCT307.7097246E-418.9948044
GCATACC307.7097246E-418.9948043
GTAACAA402.7495605E-416.6240521
AGTTCAC350.002166640216.281266
TACTAGT350.002166640216.281262
GGTATCA8650.015.70430851
ACATGGA508.6830965E-515.1958443
GAACGCG456.7123474E-414.78333813
GCTAAAA456.7344453E-414.7769361
TACATGA653.3516899E-614.6113872
ACATGAA801.2765668E-714.2461023
TAGTTCA400.00527067314.2461025
CATGGAA400.00527067314.2461024
TCATAAA400.00527067314.2461022
GACCAAA551.949745E-413.8173941
Produced by FastQC (version 0.11.5)