FastQCFastQC Report
Wed 25 May 2016
SRR1294622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231289
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT127705.521231014012772No Hit
GGTATCAACGCAGAGTACTTTTTTT96434.169242808780357No Hit
TATCAACGCAGAGTACTTTTTTTTT86293.730830259977777No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50932.2020070128713427No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26621.1509410304856695No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23801.0290156470908691No Hit
GTACTGGTTCACTATCGGTCAGTCA21280.9200610491636005No Hit
GCAGAGTACTTTTTTTTTTTTTTTT18260.7894884754571121No Hit
ATACAGGGTGACAGCCCCGTACACA17990.7778147685363332No Hit
GGATACCACGTGTCCCGCCCTACTC14730.6368655664558193No Hit
ATCAACGCAGAGTACTTTTTTTTTT14310.6187064668012746No Hit
GTTAATGATAGTGTGTCGAAACACA11310.48899861212595497No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10600.4583010865194626No Hit
GTGGTATCAACGCAGAGTACTTTTT9830.42500940381946395No Hit
ACTTAGATGTTTCAGTTCCCCCGGT9670.41809165157011363No Hit
GGTATCAACGCAGAGTACATGGGGG8960.38739412596362127No Hit
AGCGTACACGGTGGATGCCCTGGCA8060.3484817695610254No Hit
GTTCACTATCGGTCAGTCAGGAGTA7950.343725814889597No Hit
CCCATATTCAGACAGGATACCACGT7480.32340491765713025No Hit
CTATCGGTCAGTCAGGAGTATTTAG7470.32297255814154585No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG7410.32037840104803944No Hit
GCCCAGAGCCTGAATCAGTGTGTGT7260.31389300831427347No Hit
GTATTTAGCCTTGGAGGATGGTCCC7090.3065428965493387No Hit
GCCTTGGAGGATGGTCCCCCCATAT7010.30308402042466354No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6650.2875190778636251No Hit
CACACACACTGATTCAGGCTCTGGG6640.2870867183480408No Hit
TCATTAACCTATGGATTCAGTTAAT6630.2866543588324564No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6620.286221999316872No Hit
TCACGGTACTGGTTCACTATCGGTC6510.2814660446454436No Hit
CGGTACTGGTTCACTATCGGTCAGT6200.2680628996623272No Hit
TCTAAGTACCCCGAGGAAAAGAAAT6100.2637393045064832No Hit
GGTTAATGAGGCGAACCGGGGGAAC5960.2576862712883017No Hit
GTACTTAGATGTTTCAGTTCCCCCG5950.2572539117727173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5930.2563891927415485No Hit
ATCATTAACTGAATCCATAGGTTAA5880.25422739516362647No Hit
GTATCAACGCAGAGTACATGGGGGT5840.25249795710128886No Hit
CAACGCAGAGTACTTTTTTTTTTTT5620.24298604775843208No Hit
ACGCAGAGTACATGGGGGTTAAGCG5590.2416889692116789No Hit
CATCTAAGTACCCCGAGGAAAAGAA5550.23995953114934132No Hit
GTACATGGGGGTTAAGCGACTAAGC5530.23909481211817252No Hit
CCTATGGATTCAGTTAATGATAGTG5450.2356359359934973No Hit
GGGTTTCCCCATTCGGAAATCGCCG5260.22742110519739372No Hit
TATTCAGACAGGATACCACGTGTCC5100.22050335294804332No Hit
AGCTCACAGCATGTGCATTTTTGTG5070.21920627440129015No Hit
GGTTCACTATCGGTCAGTCAGGAGT5050.21834155537012134No Hit
GTGCTAATCTGCGATAAGCGTCGGT4870.21055908408960217No Hit
GGGTACTTAGATGTTTCAGTTCCCC4740.20493841038700503No Hit
CCCCAGTAGCGGCGAGCGAACGGGG4710.20364133184025182No Hit
AACGCAGAGTACTTTTTTTTTTTTT4700.2032089723246674No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4650.2010471747467454No Hit
GGGTGACAGCCCCGTACACAAAAAT4530.19585886055973264No Hit
GTTATAACCGGCGATTTCCGAATGG4090.17683504187401908No Hit
GGTATCAACGCAGAGTACATGGGGA4070.1759703228428503No Hit
GTCCCGCCCTACTCATCGAGCTCAC3990.1725114467181751No Hit
TCAACGCAGAGTACTTTTTTTTTTT3930.1699172896246687No Hit
GCCCTACTCATCGAGCTCACAGCAT3890.16818785156233113No Hit
GTGGTATCAACGCAGAGTACATGGG3820.1651613349532403No Hit
ATGATAGTGTGTCGAAACACACTGG3810.1647289754376559No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC3750.16213481834414953No Hit
GGAGTATTTAGCCTTGGAGGATGGT3730.16127009931298072No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3630.15694650415713673No Hit
CCTATGTATTCAGTATGCAATGACC3620.15651414464155233No Hit
AGTCAGGAGTATTTAGCCTTGGAGG3600.15564942561038356No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3600.15564942561038356No Hit
GATATGAACCGTTATAACCGGCGAT3590.15521706609479916No Hit
TTCCAGACGCTTCCACTAACACACA3580.15478470657921475No Hit
GGTGATATGAACCGTTATAACCGGC3490.15089347093895517No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA3430.14829931384544875No Hit
TAATGATAGTGTGTCGAAACACACT3380.14613751626752677No Hit
CACTATCATTAACTGAATCCATAGG3270.14138156159609838No Hit
CAGTAGCGGCGAGCGAACGGGGAGC3260.140949202080514No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3250.14051684256492958No Hit
TCCCCCAGTAGCGGCGAGCGAACGG3220.1392197640181764No Hit
TATCAACGCAGAGTACATGGGGGTT3190.1379226854714232No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA3170.13705796644025442No Hit
CATTAACCTATGGATTCAGTTAATG3130.1353285283779168No Hit
GTCCCCCCATATTCAGACAGGATAC3120.1348961688623324No Hit
CTGTCACCCTGTATCGCGCGCCTTT2990.12927549515973522No Hit
TCGGTAAGGTGATATGAACCGTTAT2970.12841077612856644No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2970.12841077612856644No Hit
GAGTACATGGGGGTTAAGCGACTAA2930.12668133806622883No Hit
CTACTGGGGGAATCTCGGTTGATTT2890.12495190000389124No Hit
CCCTGGCAGTCAGAGGCGATGAAGG2870.12408718097272245No Hit
ACTTAGATGTTTCAGTTCGCCGCGT2860.12365482145713805No Hit
CTGTGAGCTCGATGAGTAGGGCGGG2750.11889886678570964No Hit
AACACACACACTGATTCAGGCTCTG2740.11846650727012524No Hit
CAGTTAATGATAGTGTGTCGAAACA2710.11716942872337206No Hit
CGTCTGGAAAGGCGCGCGATACAGG2690.11630470969220326No Hit
GTATCGCGCGCCTTTCCAGACGCTT2670.11543999066103447No Hit
GGGCTGTCACCCTGTATCGCGCGCC2660.11500763114545007No Hit
GCGTACACGGTGGATGCCCTGGCAG2640.11414291211428126No Hit
CTAACACACACACTGATTCAGGCTC2600.11241347405194367No Hit
CCGTTATAACCGGCGATTTCCGAAT2560.11068403598960609No Hit
GGTATCAACGCAGAGTACATGGGCA2520.10895459792726848No Hit
CACTGATTCAGGCTCTGGGCTGCTC2490.10765751938051529No Hit
GTGGTATCCTGTCTGAATATGGGGG2450.10592808131817769No Hit
CTATCATTAACTGAATCCATAGGTT2440.10549572180259328No Hit
TAAGTACCCCGAGGAAAAGAAATCA2430.10506336228700888No Hit
CCGCTACTGGGGGAATCTCGGTTGA2390.1033339242246713No Hit
GTACGGGGCTGTCACCCTGTATCGC2350.1016044861623337No Hit
AAGTACCCCGAGGAAAAGAAATCAA2330.1007397671311649No Hit
CTACTCATCGAGCTCACAGCATGTG2330.1007397671311649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAGAC250.00591874319.054137
GTACATA453.484573E-516.90761
GGTATCA14350.016.2374821
ACGCAAA704.3338514E-714.9451111
CGCAAAA456.741407E-414.7749132
AACCAGA400.00522255914.2657871
CCAGAAC400.005267868314.2472373
ACCAGAA400.005267868314.2472372
ATCGCCG801.3095269E-714.21642519
CAAAGAA706.989945E-613.6100934
CCAAAAC1205.0931703E-1113.4557253
GGTTATA852.6297676E-713.4266241
AATCGCC852.736033E-713.383059518
AAGGCGA500.00151959713.26866319
ACAGGGT2900.013.1009073
TACAGGG3150.012.6642112
CCTGACT853.9970764E-612.26780418
AGGGTGA3200.012.2065525
CAGGGTG3150.012.097864
AAATCGC951.0540334E-611.97431617
Produced by FastQC (version 0.11.5)