FastQCFastQC Report
Wed 25 May 2016
SRR1294621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047983
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131220.6407279747927594No Hit
CCCATGTACTCTGCGTTGATACCAC88190.4306188088475344No Hit
GTACATGGGAAGCAGTGGTATCAAC87260.4260777555282441No Hit
GGTATCAACGCAGAGTACTTTTTTT80590.3935091258081732No Hit
TATCAACGCAGAGTACTTTTTTTTT78340.3825227064873097No Hit
ATTCCATTCCATTCCATTCCATTCC73040.3566435854203868No Hit
GAATGGAATGGAATGGAATGGAATG53460.26103732306371685No Hit
GTACTGGTTCACTATCGGTCAGTCA41130.20083174518538485No Hit
GAGTACATGGGAAGCAGTGGTATCA40600.19824383307869256No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34140.16670060249523555No Hit
CATGTACTCTGCGTTGATACCACTG32970.16098766444838652No Hit
GTTAATGATAGTGTGTCGAAACACA28840.14082148142831263No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27460.13408314424484968No Hit
GCGTTGATACCACTGCTTCCCATGT25590.12495220907595424No Hit
GGTATCAACGCAGAGTACATGGGAA25350.12378032434839548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA25110.1226084396208367No Hit
GTATCAACGCAGAGTACATGGGAAG24730.12075295546886865No Hit
GGATACCACGTGTCCCGCCCTACTC23220.11337984739131135No Hit
ACGCAGAGTACATGGGAAGCAGTGG22800.1113290491180835No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21920.10703213845036799No Hit
TATCAACGCAGAGTACATGGGAAGC21660.10576259666217933No Hit
ATACAGGGTGACAGCCCCGTACACA20980.1024422566007628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT551.9547745E-413.82305412
AATCGCC2200.013.39534218
TATTCCG2200.012.950885
ATCGCCG2300.012.80385519
CCGTTCA1207.4396667E-1012.6627749
CGCCGGT604.1070225E-412.66153611
AAATCGC2400.011.88034817
CCTAGTA802.8742767E-511.8722212
TGTCCCG4750.011.7952211
CGCCCTA5200.011.69757516
GGACCGG658.0460473E-411.6887146
CCGCCCT5400.011.6154915
CGCCTTT4600.011.36297914
CGCGCCT4200.011.31351212
CGTTCAA1354.7566573E-911.25524910
GTATTAG1950.011.2055171
CCCGCCC5350.011.19113414
CCGGTTA600.005860268611.08751213
CTAGGAC1201.280423E-711.0804683
GTACTAG1408.35098E-910.857521
Produced by FastQC (version 0.11.5)