##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294621_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2047983
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.72189124616757	31.0	30.0	33.0	25.0	34.0
2	29.73933621519319	31.0	30.0	34.0	24.0	34.0
3	29.692591686552085	31.0	30.0	34.0	23.0	34.0
4	33.1702763157702	35.0	33.0	37.0	25.0	37.0
5	33.68759506304496	35.0	35.0	37.0	28.0	37.0
6	33.705462887143106	37.0	35.0	37.0	27.0	37.0
7	33.61704564930471	36.0	35.0	37.0	27.0	37.0
8	33.425548942544935	36.0	35.0	37.0	26.0	37.0
9	34.457455945679236	38.0	34.0	39.0	23.0	39.0
10	34.632023800978814	38.0	34.0	39.0	25.0	39.0
11	34.87520013593863	39.0	35.0	39.0	27.0	39.0
12	34.68301982975444	38.0	34.0	39.0	26.0	39.0
13	34.49698703553692	38.0	34.0	39.0	25.0	39.0
14	35.34921285967706	39.0	34.0	41.0	24.0	41.0
15	35.334234219717644	39.0	34.0	41.0	24.0	41.0
16	35.467827125518134	39.0	34.0	41.0	25.0	41.0
17	35.49792942617199	39.0	34.0	41.0	25.0	41.0
18	35.431344400808015	39.0	34.0	41.0	25.0	41.0
19	35.743474433137386	39.0	34.0	41.0	26.0	41.0
20	35.865235209471955	39.0	34.0	41.0	26.0	41.0
21	35.923338719120224	39.0	34.0	41.0	26.0	41.0
22	35.94964997268044	39.0	34.0	41.0	27.0	41.0
23	35.958346822214835	39.0	34.0	41.0	27.0	41.0
24	35.99782127097735	39.0	34.0	41.0	27.0	41.0
25	34.90090200944051	38.0	32.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9862752086489834
1101	2	0.9918782282959242
1101	3	1.225629670952685
1101	4	0.9599619030480966
1101	5	0.6555976457635495
1101	6	0.7242038625144076
1101	7	0.7445611839248585
1101	8	0.8719464727367949
1101	9	1.0242054842381307
1101	10	1.0362813538636573
1101	11	0.8409519547777933
1101	12	0.9500730276888376
1101	13	1.1006168275489827
1101	14	1.1586790971301681
1101	15	1.2949634551420814
1101	16	1.1794137582859747
1101	17	1.1909734717940594
1101	18	1.212248096480998
1101	19	1.0505335912770235
1101	20	0.9957406519220484
1101	21	0.9627249433574576
1101	22	0.9135283702618366
1101	23	1.0323446630851905
1101	24	0.9814724875722476
1101	25	0.9305837181650674
1102	1	0.8086700013035468
1102	2	0.7726552918714269
1102	3	1.1152447848172429
1102	4	0.8931866066706462
1102	5	0.7055928840810708
1102	6	0.5722099175156217
1102	7	0.5441653088111948
1102	8	0.456131111431219
1102	9	0.8531109846437914
1102	10	0.8606158471793748
1102	11	0.6990505570488494
1102	12	0.8511416352445593
1102	13	0.8404503580199005
1102	14	1.0904039921563964
1102	15	1.098138807203128
1102	16	1.0822389515468203
1102	17	1.0252967472378671
1102	18	1.1018691754539702
1102	19	0.8357516087066585
1102	20	0.26909673913831256
1102	21	0.5302574963759952
1102	22	0.5631613461580116
1102	23	0.6776355255864601
1102	24	0.7568912884703138
1102	25	0.8066909827446054
1103	1	0.6040314984538391
1103	2	0.6129265770491443
1103	3	0.9359779427421628
1103	4	0.7900415100445244
1103	5	0.4850042270862147
1103	6	0.4562812295294236
1103	7	0.30624168018358944
1103	8	0.4660706058091719
1103	9	0.5679678931489889
1103	10	0.5977220168807875
1103	11	0.44693728336086735
1103	12	0.7783930660513008
1103	13	0.6801335218422722
1103	14	0.5839176141723712
1103	15	0.8858019067271101
1103	16	0.7421514204770219
1103	17	0.7724495392182078
1103	18	0.8578715842085884
1103	19	0.6965353845647897
1103	20	-0.17537130861258987
1103	21	0.23142888316290566
1103	22	-0.37542143016434437
1103	23	0.27960099793934745
1103	24	0.41802977671167696
1103	25	0.3893904083659194
1104	1	0.3358086354229606
1104	2	0.2605266581614707
1104	3	0.5683066594855788
1104	4	0.48403967703372786
1104	5	0.3943002636527595
1104	6	0.2171019276489332
1104	7	0.2624971754903598
1104	8	0.39240395617396473
1104	9	0.6968768791597739
1104	10	0.5328282985087398
1104	11	0.31170496893457766
1104	12	0.24489311714567918
1104	13	0.2568295092304709
1104	14	0.621867430977737
1104	15	0.5130116055044667
1104	16	0.4972555359162527
1104	17	0.391069527977443
1104	18	0.376353378643131
1104	19	-0.28530428979371436
1104	20	0.2010775629467858
1104	21	-0.4153688565495557
1104	22	-0.06869775601022354
1104	23	-0.29376573834341
1104	24	-0.6767050541127091
1104	25	-1.902713088618448
1105	1	-0.012661860571881789
1105	2	-0.010201849440257149
1105	3	0.3855725707263389
1105	4	0.12408395010179163
1105	5	0.018778526656497263
1105	6	-0.01920128313953029
1105	7	0.033188502866963177
1105	8	0.17579728557599594
1105	9	0.5031655118413667
1105	10	-1.6484165193726739
1105	11	-0.559575162844375
1105	12	0.07321658696657352
1105	13	0.12494865675375877
1105	14	0.42657740061233795
1105	15	-0.44724459617703616
1105	16	-0.011562230032431842
1105	17	-0.10264787863334845
1105	18	-0.6572462378218944
1105	19	-1.7615422682430193
1105	20	-0.5208837251890515
1105	21	-1.6014737085090758
1105	22	-0.9181268899025312
1105	23	-0.37178594656065655
1105	24	-0.5874840869133848
1105	25	-0.7672837527956133
1106	1	-0.254364739739497
1106	2	-0.2936173891262861
1106	3	0.230372987663916
1106	4	0.18914223465088753
1106	5	-0.0932324920651908
1106	6	-0.2588044723399605
1106	7	-0.19120551094633953
1106	8	-0.10261273784823999
1106	9	0.0596750158038617
1106	10	-0.2978226023527313
1106	11	-0.3779240018190393
1106	12	-0.12347085056855178
1106	13	-0.2109363289627666
1106	14	-0.4939115439109045
1106	15	-0.5473337684917325
1106	16	-0.3563030887450225
1106	17	-0.1083046348313772
1106	18	-0.1573336248900432
1106	19	-0.30567108743294824
1106	20	-0.2949796778401108
1106	21	-0.39436973244998086
1106	22	-0.4764992761290685
1106	23	-0.8052413317493503
1106	24	-0.45829134539578575
1106	25	-0.563738568205622
1107	1	0.4285733531652127
1107	2	0.5313299376101099
1107	3	0.727210241674193
1107	4	0.6212721616427501
1107	5	0.4067698581083121
1107	6	0.5074581407474525
1107	7	0.25364355503595704
1107	8	0.45994594304637815
1107	9	0.6328262224416932
1107	10	-1.3054885745431264
1107	11	-0.20801233309009604
1107	12	0.2639843345604902
1107	13	0.5193653980622273
1107	14	0.6671825676118814
1107	15	0.6692109241702013
1107	16	0.6177404200170145
1107	17	0.19532867464769055
1107	18	-0.24802711408688793
1107	19	0.3458366663769823
1107	20	0.35821021265355313
1107	21	0.38518340556043285
1107	22	0.41212923312063054
1107	23	0.39805983945962
1107	24	0.41268060410838814
1107	25	0.22179517382288338
1108	1	0.16826878676199186
1108	2	0.21946952730601765
1108	3	0.613593050978114
1108	4	0.10795877994513603
1108	5	0.37655065421046885
1108	6	0.23427981129332665
1108	7	0.1584964515859255
1108	8	0.29006043075424515
1108	9	0.5913387542514457
1108	10	0.5465477978269462
1108	11	0.3854966334361052
1108	12	0.18328559877864592
1108	13	0.33289284626097526
1108	14	0.46071178973228655
1108	15	0.18180663995008217
1108	16	0.3362564249261908
1108	17	0.36421249423985813
1108	18	0.5248299412131274
1108	19	0.28499289755086465
1108	20	0.2671177160930185
1108	21	0.3117456649785382
1108	22	0.15388186771817658
1108	23	0.25324043813824204
1108	24	0.29747410916331773
1108	25	0.12984187679515458
1109	1	-0.16687225688001206
1109	2	-0.26734937848818774
1109	3	0.14060548438795095
1109	4	-0.009438012528477202
1109	5	-0.01975827424249843
1109	6	-0.010831696229466559
1109	7	-0.22044313296083828
1109	8	-0.3365150774550685
1109	9	-0.1700182274529567
1109	10	0.019567073294318504
1109	11	-0.28144202358035386
1109	12	-0.1601911670132523
1109	13	-0.10760754538321748
1109	14	0.2105823067452235
1109	15	-0.7448400154328496
1109	16	-0.29476185606314687
1109	17	-0.09124858387414037
1109	18	0.09774903853868722
1109	19	-0.030433014748837195
1109	20	-0.06922333509582046
1109	21	0.056767705995582673
1109	22	-0.0683934663661887
1109	23	-0.06853120560491277
1109	24	0.04226481535835802
1109	25	-0.046557650188738364
1110	1	-0.07709749027988622
1110	2	0.008023090912736563
1110	3	0.08904559942434886
1110	4	-0.07920623838696628
1110	5	-0.02074064574654244
1110	6	-0.1457740646080694
1110	7	-0.19669400253920344
1110	8	-0.060816453153250905
1110	9	0.36426313534017396
1110	10	0.3521471501196771
1110	11	-0.08104182849233865
1110	12	0.09245524981976416
1110	13	0.045445241804486614
1110	14	0.10918978411098124
1110	15	0.1456672764228557
1110	16	-0.1408091812904999
1110	17	0.16357102272356627
1110	18	-5.213258374301834
1110	19	-2.123757614490735
1110	20	-0.7986204400299854
1110	21	-0.5374058668876884
1110	22	-0.4041879585324182
1110	23	-0.36330403140694045
1110	24	-0.08353135295168812
1110	25	-0.24096128326195299
1111	1	-0.5953187579225627
1111	2	-0.4682833985235746
1111	3	-1.605487088837247
1111	4	-1.080673813121777
1111	5	-0.7469586478505121
1111	6	-0.8518950319965128
1111	7	-0.8759071501678548
1111	8	-2.1076739200595362
1111	9	-0.5364609266393074
1111	10	-0.4526925316546766
1111	11	-0.6393242975902567
1111	12	-0.2866239340147203
1111	13	-1.0559993207448244
1111	14	-0.436237091930046
1111	15	-0.5237591174580984
1111	16	-2.411197977643617
1111	17	-2.280873726114862
1111	18	-0.951381073083283
1111	19	-0.5968681496884187
1111	20	-0.4263679306059487
1111	21	-0.4246110917496182
1111	22	-0.4219276140071315
1111	23	-0.5789320449074253
1111	24	-0.5162949520574998
1111	25	-0.5661549691565781
1112	1	-0.2877166315421569
1112	2	-0.36666013490935967
1112	3	0.06951844861173129
1112	4	-0.50621410992904
1112	5	-1.9112310270550203
1112	6	-0.9399657847731504
1112	7	-0.6588881962710715
1112	8	-0.19170233508342704
1112	9	-0.5991116243578816
1112	10	-0.30207075524104
1112	11	-0.8213323348019159
1112	12	-1.4635849820164921
1112	13	-1.4679649569185997
1112	14	-2.8619308551803684
1112	15	-3.994231857387849
1112	16	-1.2603483747578181
1112	17	-0.6723713687437254
1112	18	-0.28068525306619563
1112	19	-0.15610619670927406
1112	20	-0.2870915960807423
1112	21	-0.26537304472876144
1112	22	-0.33807323309392245
1112	23	-0.1691750598110744
1112	24	-0.24835519950236318
1112	25	-0.08586828751243303
1113	1	-1.0029586065075549
1113	2	-0.5175440152660151
1113	3	-1.1154826158188946
1113	4	-3.2075483082381773
1113	5	-1.0868446192632533
1113	6	-1.1646237734760803
1113	7	-0.8734700227802961
1113	8	-1.4243499333317189
1113	9	-3.156982400465477
1113	10	-1.0095773080371373
1113	11	-1.3294042110673985
1113	12	-2.743445954617311
1113	13	-2.1079641107466216
1113	14	-1.4713489507695954
1113	15	-0.7658805966251805
1113	16	-0.8047922147937072
1113	17	-0.8109965004544293
1113	18	-0.6662995907116525
1113	19	-0.4347472713745475
1113	20	-0.3906375267658291
1113	21	-0.5035291554000025
1113	22	-0.45508169279901267
1113	23	-0.36310256704886257
1113	24	-0.48169048037437534
1113	25	-0.470105245215251
1201	1	1.07215872909482
1201	2	1.1131444103940424
1201	3	1.319842073649749
1201	4	1.0009731512566944
1201	5	0.8395437225773108
1201	6	0.7254477420265175
1201	7	0.7278297974537793
1201	8	0.881594903484185
1201	9	1.1889166747138304
1201	10	1.0550802965025454
1201	11	0.8165820243614945
1201	12	1.1014143133712224
1201	13	1.010132181117605
1201	14	1.2958013901375551
1201	15	1.3125868462320724
1201	16	1.1531897965869078
1201	17	1.224772943480552
1201	18	1.2868987962391003
1201	19	1.055931359197288
1201	20	0.9363134647195963
1201	21	0.9386499243360973
1201	22	0.9643729158868766
1201	23	0.9394398797254198
1201	24	1.0736240398416896
1201	25	1.1531521761843493
1202	1	0.9491799820122822
1202	2	0.9433307142061551
1202	3	1.1843033100809421
1202	4	1.0218954904617235
1202	5	0.773171661326451
1202	6	0.7433997003164734
1202	7	0.7109512922686818
1202	8	0.8825744049758057
1202	9	1.1364077192516504
1202	10	0.95743101619707
1202	11	0.7843495606677777
1202	12	1.0450805346065835
1202	13	0.9825986262709776
1202	14	1.08986469674781
1202	15	1.4641903570025363
1202	16	1.3164835024719395
1202	17	1.312739894882121
1202	18	1.3601162531199265
1202	19	1.124915610837732
1202	20	1.1234445962856867
1202	21	0.933490205195497
1202	22	0.7167656062276748
1202	23	0.7795042449690257
1202	24	0.7528273049957903
1202	25	0.7181126563889393
1203	1	0.5378177493909533
1203	2	0.5031732074716189
1203	3	0.6928413501279991
1203	4	0.5544853881734184
1203	5	0.3437401838089187
1203	6	0.25655879156794015
1203	7	0.41029879175854234
1203	8	0.4893274326082988
1203	9	0.7402356902893672
1203	10	0.4695416831321566
1203	11	0.3158400200133471
1203	12	0.5772100733163157
1203	13	0.699876023554701
1203	14	0.8194129245402593
1203	15	0.8228669224491085
1203	16	0.7969467648238435
1203	17	0.7699676613158388
1203	18	0.8362213406929015
1203	19	0.6173404695390872
1203	20	-0.19714364706434395
1203	21	0.2740362404109362
1203	22	0.3586419783001844
1203	23	0.27247108515194185
1203	24	0.5533821598214459
1203	25	0.4977323038510377
1204	1	0.18682554868564338
1204	2	0.2296866621063316
1204	3	0.49927264090772994
1204	4	0.38585034735280743
1204	5	0.0620949780435609
1204	6	0.14399707510944637
1204	7	0.0638706502335964
1204	8	0.28852312507514455
1204	9	-0.7572143911562961
1204	10	-0.6293855808836284
1204	11	-0.14983994759046482
1204	12	0.05738234497090389
1204	13	0.16073911492928517
1204	14	-1.6708042560270115
1204	15	-0.23145617153524256
1204	16	0.020564692108429483
1204	17	-0.0569165894093544
1204	18	0.3992385021083962
1204	19	0.27242012646588165
1204	20	-0.8592669124032142
1204	21	-0.7657004764943238
1204	22	-1.6947984476786715
1204	23	-1.388203522986693
1204	24	-0.35906221823613294
1204	25	-3.0741275199188216
1205	1	-0.22800920773483568
1205	2	-0.25185000852178163
1205	3	0.1763174703094741
1205	4	0.24007191693525698
1205	5	0.2599513827448945
1205	6	0.16130102743725416
1205	7	0.29741819953863313
1205	8	0.3170689520166263
1205	9	0.7433447834841402
1205	10	0.7501897754142419
1205	11	0.49929055473581485
1205	12	0.6005101478241741
1205	13	0.5703522776254601
1205	14	0.7120017607630018
1205	15	0.22890870203872993
1205	16	-0.446596211422559
1205	17	-0.7278342718001625
1205	18	-0.36515373293768505
1205	19	-0.4643630655128206
1205	20	0.16739170430646055
1205	21	-0.19397134051627773
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2111	22	-0.025159746165911656
2111	23	0.05133400342062089
2111	24	-0.08866386944715998
2111	25	0.06775814908958466
2112	1	-1.1144543048762259
2112	2	-0.4803859649209983
2112	3	-0.2730452075582974
2112	4	-0.050394462887958014
2112	5	-0.6565459993373253
2112	6	-0.6952105400179036
2112	7	-0.46393519706557385
2112	8	-0.08835713667063771
2112	9	-2.45032355613386
2112	10	-0.7762588619524848
2112	11	-1.118982745221878
2112	12	-0.8854131739404352
2112	13	-0.383074589802618
2112	14	-0.6401017910999371
2112	15	-0.8265562557124113
2112	16	-1.0802040300455573
2112	17	-1.1600172720840334
2112	18	-0.3105997986107738
2112	19	-0.27294801678169733
2112	20	-0.2696028891005682
2112	21	-0.27403046391080466
2112	22	-0.1520380339834091
2112	23	-0.37975963535782853
2112	24	-0.25786494064631427
2112	25	-0.01187213963070377
2113	1	0.1939400713442474
2113	2	-0.11692574424237989
2113	3	0.08635584817117348
2113	4	0.3111950265567245
2113	5	0.11867652443176979
2113	6	-0.5696188707215981
2113	7	-0.16146518817981104
2113	8	0.22297990720762328
2113	9	-0.7072497177861621
2113	10	0.015948531586651882
2113	11	-0.23149646006665137
2113	12	-0.252176757743257
2113	13	0.10106223648657675
2113	14	0.38552351339932045
2113	15	-1.6943072753310702
2113	16	-0.36187946255003567
2113	17	-1.3220522216675334
2113	18	0.011490360237168318
2113	19	0.2388547541780781
2113	20	0.21330323204426094
2113	21	0.2127260462668943
2113	22	0.28381560700255193
2113	23	0.453255692920429
2113	24	0.33371795709078356
2113	25	0.6537890743261556
2114	1	-0.6650729429779219
2114	2	-0.4729888203024757
2114	3	-2.9845464321573587
2114	4	-1.2394873908444701
2114	5	-0.9974122310342963
2114	6	-1.3559303185475855
2114	7	-3.102025070281286
2114	8	-1.1995509566603673
2114	9	-0.7496311235511612
2114	10	-0.5735259403795894
2114	11	-0.5505289125421342
2114	12	-0.32195900985317394
2114	13	-2.9336530085447095
2114	14	-2.311557725482537
2114	15	-1.1271878444478958
2114	16	-1.351804158876213
2114	17	-1.2082617613882576
2114	18	-0.2267870773238556
2114	19	-0.17471568981181917
2114	20	-0.08355617544052762
2114	21	-0.026411771754290214
2114	22	-0.1368989843958346
2114	23	9.250862361653844E-5
2114	24	-0.12566269904444027
2114	25	0.009139016897897534
2201	1	1.2814379187740883
2201	2	1.226984838641421
2201	3	1.48157734732683
2201	4	1.1641547676611879
2201	5	0.9492107289911331
2201	6	0.8502440054366431
2201	7	0.9091936433963284
2201	8	0.9745223350658918
2201	9	1.348485519471602
2201	10	1.279876340049853
2201	11	0.9713459445325654
2201	12	1.1951973659338293
2201	13	1.3493895880654563
2201	14	1.6941880651457737
2201	15	1.6766984681735622
2201	16	1.5060328107756433
2201	17	1.604729865971322
2201	18	1.7043383575461135
2201	19	1.3520771427142577
2201	20	1.2569757964952828
2201	21	1.2799819142839652
2201	22	1.3420119878076235
2201	23	1.214941608450026
2201	24	1.224547878581248
2201	25	1.4673571369845817
2202	1	1.1245744862935467
2202	2	1.0674188618081502
2202	3	1.2837659008009155
2202	4	1.1026728728548676
2202	5	0.8591850620740473
2202	6	0.7877648397620831
2202	7	0.8319793598340226
2202	8	1.067460330030805
2202	9	1.2356348034094609
2202	10	1.2910938671374268
2202	11	0.9272629068424436
2202	12	1.1770816205915224
2202	13	1.1992471475743969
2202	14	-0.8760807259406249
2202	15	0.6218546792773267
2202	16	0.9986907285412698
2202	17	1.195647564814955
2202	18	1.360366265025256
2202	19	1.3582638874113826
2202	20	1.2347505260152332
2202	21	1.1675400751126688
2202	22	1.022092269292635
2202	23	1.0727041674612323
2202	24	1.1645041336132493
2202	25	1.2184650267535915
2203	1	0.6221039862835127
2203	2	0.6738254515556577
2203	3	0.9130045469243164
2203	4	0.6116095271103248
2203	5	0.44584321523603876
2203	6	0.4577735621452419
2203	7	0.3471835972548618
2203	8	0.4130396080839702
2203	9	0.6925025572544996
2203	10	0.5041257546903566
2203	11	0.43694824710890856
2203	12	0.7515485743090977
2203	13	0.6985882929629668
2203	14	0.5979130838841158
2203	15	0.6981840645342956
2203	16	0.7413204922505585
2203	17	0.755234725368652
2203	18	0.8664599875715595
2203	19	0.712084923331247
2203	20	-0.1533261714330223
2203	21	0.3135417655266366
2203	22	-0.5162454648236192
2203	23	-0.24830226367514996
2203	24	0.23523441510908327
2203	25	0.5717194285945624
2204	1	0.4763662045788166
2204	2	0.4274042943737122
2204	3	0.6369791596778782
2204	4	0.4834882403759764
2204	5	0.37125576596676524
2204	6	0.43179787620244525
2204	7	0.19100943910122936
2204	8	0.34089664539972375
2204	9	0.7614024226372464
2204	10	-1.6982946439609563
2204	11	-0.14571454235756676
2204	12	0.23102407949269121
2204	13	0.49095728341733036
2204	14	0.7056796678183019
2204	15	0.7569600186348211
2204	16	0.5775636200607792
2204	17	0.6088746055750462
2204	18	0.7934298790502297
2204	19	0.5954612380728079
2204	20	-0.34497778812619373
2204	21	0.24718509142421397
2204	22	0.33914358222541807
2204	23	0.39920153446158224
2204	24	0.46071462342185754
2204	25	0.008341741957444526
2205	1	0.3695603135407346
2205	2	0.337240422575114
2205	3	0.5269965037733506
2205	4	0.15717560350437765
2205	5	0.12716021457048754
2205	6	0.08707251783333447
2205	7	0.1565673272264121
2205	8	0.2708141693911159
2205	9	0.5546061613529147
2205	10	0.44372518832587815
2205	11	0.28868068539467373
2205	12	0.14200392208639556
2205	13	0.18706096121920268
2205	14	0.44712459985410646
2205	15	0.05173310422396327
2205	16	0.18018814292030783
2205	17	0.399015068245852
2205	18	-0.10088581299577726
2205	19	-0.7408551453131196
2205	20	-0.10327301081984785
2205	21	-0.9388885589348348
2205	22	-1.5891857569337233
2205	23	-2.0615918650338187
2205	24	-1.2650552826360126
2205	25	-1.9673004480092757
2206	1	-0.09814799707804767
2206	2	-0.3720464676754389
2206	3	0.3479206498878895
2206	4	0.08542979630792047
2206	5	-0.07073184466422333
2206	6	-0.1502090741721105
2206	7	-0.14549806161038248
2206	8	-0.11985917474828511
2206	9	0.3580153431202149
2206	10	-0.1228789527490548
2206	11	-0.16375375434937212
2206	12	0.014025741382745593
2206	13	0.1150058575318127
2206	14	-0.14975720694015138
2206	15	-0.13767673535677716
2206	16	-0.7865004357180752
2206	17	-0.44212381822014635
2206	18	-0.07972410294734544
2206	19	-0.7017864934551241
2206	20	-0.5310309663647459
2206	21	-2.5517867753275993
2206	22	-1.3952422440887915
2206	23	-2.10553369700186
2206	24	-1.0019323284600432
2206	25	-1.8733264825208593
2207	1	0.5639136274842613
2207	2	0.4300988620356776
2207	3	0.7345108523656201
2207	4	0.37269997469542204
2207	5	0.3122779607299222
2207	6	0.36821492888633856
2207	7	0.22696619157663633
2207	8	0.28019633348925055
2207	9	-1.2182363488246963
2207	10	-0.188352481715647
2207	11	0.09214652819855473
2207	12	0.47391684336596285
2207	13	0.6108624917357091
2207	14	0.747797464188011
2207	15	0.7550695853413387
2207	16	0.7383134539594494
2207	17	0.6804868748427424
2207	18	0.7147110660069416
2207	19	-0.39282657254423725
2207	20	0.268499065820194
2207	21	-0.2998991913742799
2207	22	0.2029666746784642
2207	23	-0.3482340598891298
2207	24	-1.4636879341987523
2207	25	-0.029699742715287414
2208	1	0.39518052345408705
2208	2	-0.02843929447209348
2208	3	0.6036750351803128
2208	4	0.4108729750850273
2208	5	0.31509544959740055
2208	6	0.27863933891671877
2208	7	0.2288273476374343
2208	8	0.44784976861470227
2208	9	-0.16495790324196946
2208	10	0.03154048358531725
2208	11	0.2728184948117587
2208	12	0.24997147456473812
2208	13	0.4172827810018873
2208	14	0.6897641251977902
2208	15	0.5710998122462954
2208	16	0.4688929184991224
2208	17	0.4098748075050551
2208	18	0.6084873735249445
2208	19	-1.427504811132657
2208	20	-0.21746930181699042
2208	21	-0.226023736930955
2208	22	0.16217397732278016
2208	23	0.19727504809927154
2208	24	0.37049791609130267
2208	25	0.31650743324659913
2209	1	0.269521461365013
2209	2	-1.4537563732454544
2209	3	-0.12923599524659224
2209	4	0.28597956652630785
2209	5	0.1677179858056732
2209	6	0.26388916956583586
2209	7	0.26714951982919644
2209	8	0.27318630223055607
2209	9	-1.968096109974283
2209	10	-0.28557790264721206
2209	11	0.029520922447943576
2209	12	0.41265157835514543
2209	13	0.4708398528371376
2209	14	0.676210428660518
2209	15	0.7412822189743906
2209	16	0.697392856384262
2209	17	0.4856776713516311
2209	18	0.6892986873362403
2209	19	0.5926597815227836
2209	20	0.5063178762698257
2209	21	0.4558732977291342
2209	22	0.34096433147122696
2209	23	0.5454543795558777
2209	24	0.5484282724430756
2209	25	0.6172175349977707
2210	1	0.2288294908239763
2210	2	0.20838666547810902
2210	3	0.20209299028557481
2210	4	0.23394734979370213
2210	5	0.2377199702470989
2210	6	0.13868288292977127
2210	7	0.22264632961886122
2210	8	0.2748372924296447
2210	9	0.3688377607228617
2210	10	-1.4610176283760339
2210	11	-0.4049300030592846
2210	12	-2.0926844545001373
2210	13	-0.7535379643609588
2210	14	-0.37098233031257166
2210	15	-0.5557636820386946
2210	16	-0.0028038217444503744
2210	17	0.0882026523088939
2210	18	0.46912642201967714
2210	19	0.30191471439336226
2210	20	0.3899227186786902
2210	21	0.3034467023102678
2210	22	0.3783793014095025
2210	23	0.21124191233749912
2210	24	0.2893044893076677
2210	25	0.22616046441439863
2211	1	-0.29759358503570255
2211	2	-0.12943528431358686
2211	3	-1.5404016353317118
2211	4	-0.591858496419178
2211	5	-0.38651537487259446
2211	6	-0.46284953278778573
2211	7	-0.4355105536425228
2211	8	-0.11243149181741785
2211	9	0.11171641188296633
2211	10	0.11813328558321246
2211	11	-0.19933773656889286
2211	12	-0.33705276808999685
2211	13	-0.22288683729356507
2211	14	-0.7327662890443989
2211	15	-0.3527409815030964
2211	16	-0.12263630833048467
2211	17	-0.23334067196031327
2211	18	-0.06535574434160196
2211	19	-0.18281415112564048
2211	20	0.03783538994169078
2211	21	-0.22916847872154023
2211	22	-0.1459346561385928
2211	23	-0.5391451353792149
2211	24	-0.42354093469614895
2211	25	-0.1340738073248815
2212	1	-0.2794841211303556
2212	2	-0.2171891755079045
2212	3	-2.1895439442120015
2212	4	-1.1469846559887884
2212	5	-0.5388733811669226
2212	6	-0.45656837564832387
2212	7	-0.24126066732301155
2212	8	-0.22463594641261153
2212	9	-0.7611525323641573
2212	10	-0.13427977604707309
2212	11	-0.21023655501724647
2212	12	0.01730582388314872
2212	13	-0.3743376904794644
2212	14	-0.01967674929097285
2212	15	0.14549167348894088
2212	16	-0.9147581264699554
2212	17	-1.1314850551515576
2212	18	-0.22349551540761325
2212	19	0.021466534874534204
2212	20	-0.07862134484927452
2212	21	0.06916615463550357
2212	22	-0.1049890635625843
2212	23	0.05466102478600021
2212	24	0.05705415927017299
2212	25	0.2229344658656558
2213	1	-2.83995177032795
2213	2	-1.2481068452056405
2213	3	-2.4175490791510903
2213	4	-0.9238799416294796
2213	5	-0.7877186218652525
2213	6	-0.4618856604845547
2213	7	-0.24047639289272382
2213	8	-0.10710003362164855
2213	9	-0.22100125465578913
2213	10	-0.17980834374161958
2213	11	-0.3884620778032115
2213	12	-0.24005050326829291
2213	13	-0.5426072131582345
2213	14	-0.06109506130920295
2213	15	-0.2871582096013512
2213	16	-1.651097698396974
2213	17	-0.8780607806798457
2213	18	-0.2507073007038798
2213	19	-0.04288118843168576
2213	20	-0.08949211602354978
2213	21	0.005027704953675993
2213	22	-0.12510043363874956
2213	23	0.04794730942116843
2213	24	0.05222002269638182
2213	25	0.013326573304780709
2214	1	-1.182668263494218
2214	2	-0.8320512113876752
2214	3	-6.508746432188605
2214	4	-2.459691230144454
2214	5	-1.4016355516237056
2214	6	-0.9882526933952533
2214	7	-0.7910641423705229
2214	8	-0.724799663195796
2214	9	-0.48759915745220184
2214	10	-0.5233136292557248
2214	11	-1.0267410215170045
2214	12	-0.7413038252703572
2214	13	-5.054426411235664
2214	14	-3.937363372813145
2214	15	-2.296496846373941
2214	16	-3.081520326999815
2214	17	-3.686080908022671
2214	18	-1.5252316392112064
2214	19	-1.0747189548149407
2214	20	-0.6095737980214793
2214	21	-0.7296957868770306
2214	22	-0.7070813199791033
2214	23	-0.5914481026255842
2214	24	-0.38038840050848677
2214	25	-0.13937816397030645
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	814.0
3	87.0
4	111.0
5	239.0
6	35.0
7	42.0
8	137.0
9	416.0
10	868.0
11	1750.0
12	3454.0
13	6175.0
14	9862.0
15	14352.0
16	18814.0
17	21426.0
18	22066.0
19	21498.0
20	20628.0
21	19738.0
22	18925.0
23	18395.0
24	18350.0
25	18876.0
26	20563.0
27	22496.0
28	25861.0
29	31330.0
30	38707.0
31	48742.0
32	63158.0
33	84407.0
34	113973.0
35	157385.0
36	222250.0
37	338052.0
38	644001.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.355311509288434	23.915893323062274	13.232949374302866	20.495845793346426
2	13.637416226895526	25.105501104598275	41.06350194294	20.1935807255662
3	16.81417226275946	32.64212935707428	30.208641230399426	20.335057149766833
4	11.14958100278908	19.133171886808952	38.43122636988125	31.286020740520716
5	13.488537964557457	39.186213803295104	32.33620342212289	14.989044810024549
6	28.100303045405994	41.166436524154214	17.63463336978478	13.098627060655014
7	21.929893566549126	36.87738532251295	25.72007362343285	15.472647487505075
8	21.84324920933737	39.390023261824766	23.014793074318135	15.751934454519736
9	23.309741302016278	18.572116124587424	23.344237623955888	34.77390494944041
10	16.34151334938944	31.511507285594014	30.267720721420638	21.879258643595904
11	30.200873695283505	31.30230042473063	21.18889397564578	17.30793190434009
12	18.625748094859087	31.57557608617584	32.865067115389216	16.933608703575857
13	24.244645126489786	28.32010952334187	27.8472966310921	19.58794871907624
14	18.905190412183096	25.362899856789596	29.09360175389993	26.63830797712738
15	23.466175832509194	31.00556830308455	24.22324224170618	21.30501362270007
16	20.720323683448065	34.39288099669173	25.64925531015674	19.23754000970347
17	18.204196465696974	32.39710997656143	30.593215080227125	18.805478477514466
18	18.84782267454228	31.34799993936321	30.801584185141735	19.00259320095278
19	19.602698064601878	29.492490608374084	31.088334056517848	19.81647727050619
20	22.420230854623348	28.152312845930144	29.029353247443634	20.398103052002877
21	22.827259051082017	31.043895775078678	27.8582627844289	18.270582389410407
22	20.130257539355046	29.828478115238198	30.750512689335913	19.290751656070842
23	19.835325757138964	30.754143730094903	30.439961965927647	18.97056854683849
24	19.915929264082973	28.26504662886318	30.114856927283995	21.704167179769858
25	21.424013181361975	28.129620635164176	28.8854380545373	21.56092812893655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4120.0
1	4120.0
2	4355.5
3	4591.0
4	4591.0
5	4591.0
6	6790.5
7	8990.0
8	8990.0
9	8990.0
10	11665.0
11	14340.0
12	14340.0
13	14340.0
14	19953.0
15	25566.0
16	25566.0
17	25566.0
18	37514.5
19	49463.0
20	49463.0
21	49463.0
22	68098.0
23	86733.0
24	86733.0
25	86733.0
26	114829.5
27	142926.0
28	142926.0
29	142926.0
30	172173.5
31	201421.0
32	201421.0
33	201421.0
34	229647.5
35	257874.0
36	257874.0
37	257874.0
38	270615.5
39	283357.0
40	283357.0
41	283357.0
42	265771.0
43	248185.0
44	248185.0
45	248185.0
46	248926.5
47	249668.0
48	249668.0
49	249668.0
50	217172.0
51	184676.0
52	184676.0
53	184676.0
54	150432.0
55	116188.0
56	116188.0
57	116188.0
58	93467.0
59	70746.0
60	70746.0
61	70746.0
62	60610.5
63	50475.0
64	50475.0
65	50475.0
66	37909.5
67	25344.0
68	25344.0
69	25344.0
70	19320.0
71	13296.0
72	13296.0
73	13296.0
74	9717.0
75	6138.0
76	6138.0
77	6138.0
78	4300.5
79	2463.0
80	2463.0
81	2463.0
82	1681.5
83	900.0
84	900.0
85	900.0
86	612.0
87	324.0
88	324.0
89	324.0
90	229.5
91	135.0
92	135.0
93	135.0
94	87.0
95	39.0
96	39.0
97	39.0
98	32.0
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10278405631296743
2	0.052978955391719554
3	0.057031723407860314
4	0.05234418449762522
5	0.0492191585574685
6	0.06958065569880219
7	0.06601617298581092
8	0.0625981758637645
9	0.06777400007714908
10	0.06884822774407796
11	0.062207547621244905
12	0.06269583292439439
13	0.06328177528817377
14	0.06562554474329133
15	0.06831111391061352
16	0.06308646116691398
17	0.06621148710707071
18	0.1474621615511457
19	0.07221739633580943
20	0.0647954597279372
21	0.06318411822754388
22	0.06943417010785734
23	0.06821345684998362
24	0.07866276233738269
25	0.07114316866888055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2047983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.324406602516326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.59844933142074	51.26625298139904
2	10.634298615836023	13.042841045002096
3	2.8263818147286193	5.1997856286112745
4	1.0394961193324483	2.5498593073472353
5	0.4897754253582963	1.5017593664296274
6	0.2713999508073961	0.9986064561129413
7	0.20213949223743918	0.8677259088676441
8	0.1465326966191899	0.7188824534430697
9	0.10941660389953581	0.6038917475941447
>10	0.5792799931760443	6.484545005839899
>50	0.05216210108556138	2.2210077728671096
>100	0.04122963673393666	5.167354191705286
>500	0.005082117796457178	2.179131531998072
>1k	0.003791421213229955	4.269267431168344
>5k	4.840112187102071E-4	2.2799503756351216
>10k+	8.066853645170117E-5	0.6491387959790849
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13122	0.6407279747927594	No Hit
CCCATGTACTCTGCGTTGATACCAC	8819	0.4306188088475344	No Hit
GTACATGGGAAGCAGTGGTATCAAC	8726	0.4260777555282441	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8059	0.3935091258081732	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7834	0.3825227064873097	No Hit
ATTCCATTCCATTCCATTCCATTCC	7304	0.3566435854203868	No Hit
GAATGGAATGGAATGGAATGGAATG	5346	0.26103732306371685	No Hit
GTACTGGTTCACTATCGGTCAGTCA	4113	0.20083174518538485	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4060	0.19824383307869256	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3414	0.16670060249523555	No Hit
CATGTACTCTGCGTTGATACCACTG	3297	0.16098766444838652	No Hit
GTTAATGATAGTGTGTCGAAACACA	2884	0.14082148142831263	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2746	0.13408314424484968	No Hit
GCGTTGATACCACTGCTTCCCATGT	2559	0.12495220907595424	No Hit
GGTATCAACGCAGAGTACATGGGAA	2535	0.12378032434839548	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2511	0.1226084396208367	No Hit
GTATCAACGCAGAGTACATGGGAAG	2473	0.12075295546886865	No Hit
GGATACCACGTGTCCCGCCCTACTC	2322	0.11337984739131135	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2280	0.1113290491180835	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2192	0.10703213845036799	No Hit
TATCAACGCAGAGTACATGGGAAGC	2166	0.10576259666217933	No Hit
ATACAGGGTGACAGCCCCGTACACA	2098	0.1024422566007628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.8828530314948904E-5	0.0
4	0.0	0.0	0.0	4.8828530314948904E-5	0.0
5	0.0	0.0	0.0	4.8828530314948904E-5	0.0
6	0.0	0.0	0.0	4.8828530314948904E-5	0.0
7	0.0	0.0	0.0	4.8828530314948904E-5	0.0
8	0.0	0.0	0.0	4.8828530314948904E-5	0.0
9	0.0	0.0	0.0	9.765706062989781E-5	0.0
10	0.0	0.0	0.0	9.765706062989781E-5	0.0
11	0.0	0.0	0.0	9.765706062989781E-5	0.0
12	0.0	0.0	0.0	9.765706062989781E-5	0.0
13	0.0	0.0	0.0	9.765706062989781E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGTT	55	1.9547745E-4	13.823054	12
AATCGCC	220	0.0	13.395342	18
TATTCCG	220	0.0	12.95088	5
ATCGCCG	230	0.0	12.803855	19
CCGTTCA	120	7.4396667E-10	12.662774	9
CGCCGGT	60	4.1070225E-4	12.661536	11
AAATCGC	240	0.0	11.880348	17
CCTAGTA	80	2.8742767E-5	11.872221	2
TGTCCCG	475	0.0	11.79522	11
CGCCCTA	520	0.0	11.697575	16
GGACCGG	65	8.0460473E-4	11.688714	6
CCGCCCT	540	0.0	11.61549	15
CGCCTTT	460	0.0	11.362979	14
CGCGCCT	420	0.0	11.313512	12
CGTTCAA	135	4.7566573E-9	11.255249	10
GTATTAG	195	0.0	11.205517	1
CCCGCCC	535	0.0	11.191134	14
CCGGTTA	60	0.0058602686	11.087512	13
CTAGGAC	120	1.280423E-7	11.080468	3
GTACTAG	140	8.35098E-9	10.85752	1
>>END_MODULE