FastQCFastQC Report
Wed 25 May 2016
SRR1294621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047983
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT302701.4780396126335034No Hit
GGTATCAACGCAGAGTACTTTTTTT206491.00826032247338No Hit
TATCAACGCAGAGTACTTTTTTTTT202950.9909750227418881No Hit
ACGCAGAGTACTTTTTTTTTTTTTT109630.5353071778427848No Hit
CCCATGTACTCTGCGTTGATACCAC96120.4693398333872888No Hit
GTACATGGGAAGCAGTGGTATCAAC85000.4150425076770657No Hit
ATTCCATTCCATTCCATTCCATTCC68960.3367215450518876No Hit
GTACTTTTTTTTTTTTTTTTTTTTT68600.33496371796054947No Hit
GAATGGAATGGAATGGAATGGAATG60890.2973169210877239No Hit
GAGTACTTTTTTTTTTTTTTTTTTT56140.27412336918812313No Hit
GAGTACATGGGAAGCAGTGGTATCA41730.20376145700428178No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40070.19565592097200027No Hit
GTACTGGTTCACTATCGGTCAGTCA38560.18828281289444299No Hit
CATGTACTCTGCGTTGATACCACTG38200.1865249858031048No Hit
ATCAACGCAGAGTACTTTTTTTTTT31030.15151492956728643No Hit
GCGTTGATACCACTGCTTCCCATGT28240.1378917696094157No Hit
ACGCAGAGTACATGGGAAGCAGTGG27810.1357921428058729No Hit
GTTAATGATAGTGTGTCGAAACACA27630.13491322926020383No Hit
GGTATCAACGCAGAGTACATGGGAA27530.13442494395705432No Hit
GTATCAACGCAGAGTACATGGGAAG25340.12373149581808053No Hit
TATCAACGCAGAGTACATGGGAAGC24960.12187601166611248No Hit
GGATACCACGTGTCCCGCCCTACTC23440.11445407505824023No Hit
ATACAGGGTGACAGCCCCGTACACA23330.11391696122477579No Hit
ACTCTGCGTTGATACCACTGCTTCC22190.10835050876887162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22140.10810636611729688No Hit
GTGGTATCAACGCAGAGTACTTTTT21730.10610439637438397No Hit
ACTTAGATGTTTCAGTTCCCCCGGT21090.10297937043422724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTT400.00534398914.2240913
GTAAGAC550.003068157212.0913613
GCTCTAG951.0291242E-612.0107311
ATCGCCG1353.8198777E-1011.94179419
GGCATAC752.057275E-411.4101941
GGAACGA1751.8189894E-1211.379271515
TAGCCAG1201.2390228E-711.1068415
TCCGGGT600.00595687711.06318118
AAATCGC1553.237801E-1011.01219817
AATCGCC1553.237801E-1011.01219818
CCTTTAG1451.4239049E-810.4921321
TTAGACA2151.8189894E-1210.1843444
CGCAAAA1409.685937E-810.1789522
TAGACAG3600.010.0490465
GTATTAG1803.8744474E-1010.0367451
GAAATCG1804.0745363E-1010.00954416
AGAACGG1054.011439E-59.9734895
CTCCTAG1054.0657993E-59.9612811
TAGCACC1252.5018035E-69.9009544
TGTCCCG5000.09.86878211
Produced by FastQC (version 0.11.5)