FastQCFastQC Report
Wed 25 May 2016
SRR1294620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365614
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21830.5970777924258918No Hit
GTACTGGTTCACTATCGGTCAGTCA15330.419294665959181No Hit
GGTATCAACGCAGAGTACTTTTTTT15190.4154654909275903No Hit
TATCAACGCAGAGTACTTTTTTTTT13780.3769002281094269No Hit
GGATACCACGTGTCCCGCCCTACTC9090.2486228645511386No Hit
ATACAGGGTGACAGCCCCGTACACA8320.22756240187738982No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6410.1753215139464025No Hit
GGTATCAACGCAGAGTACATGGGGG6080.16629560137193872No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5890.16109886382906563No Hit
ATCATTAACTGAATCCATAGGTTAA5850.1600048138200397No Hit
GTTCACTATCGGTCAGTCAGGAGTA5460.14933782623203706No Hit
GTATTTAGCCTTGGAGGATGGTCCC5220.14277352617788158No Hit
TATTCAGACAGGATACCACGTGTCC4970.13593571362146964No Hit
CTATCGGTCAGTCAGGAGTATTTAG4860.1329270760966484No Hit
GGTTAATGAGGCGAACCGGGGGAAC4850.1326535635943919No Hit
CCCATATTCAGACAGGATACCACGT4670.1277303385537753No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4170.11405471344095139No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4140.11323417593418195No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4080.11159310092064309No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4060.11104607591613012No Hit
GCCTTGGAGGATGGTCCCCCCATAT3990.10913148840033479No Hit
GTTAATGATAGTGTGTCGAAACACA3950.10803743839130886No Hit
GGTATCAACGCAGAGTACATGGGGA3810.10420826335971818No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3680.10065260083038396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3670.10037908832812747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAA250.00603623918.99301710
GGTATCA4500.016.256261
CGAATGG653.358342E-614.61401519
GTGCTAG400.005267358414.2506161
CCAAGAC400.005276943614.2467133
AGTTGTG400.005281741314.2447627
CCGAATG707.1720006E-613.58131918
CTAATAC500.001498320413.2969323
CAAGATA500.001499867913.2951134
GTCCAGG657.9947436E-411.6928141
GTACTGG3100.011.6456651
GTGGTAT1157.0027454E-811.5657191
TACTGGT2750.011.3973712
AACCGGG855.27234E-511.18154914
ACCGGGG855.27234E-511.18154915
TTCCGAA855.27234E-511.18154916
TTTCCGA855.27234E-511.18154915
TCCAAGA855.309278E-511.1738922
TGGACTC600.005877142311.0792595
TGAGCTG600.005877142311.0792595
Produced by FastQC (version 0.11.5)