FastQCFastQC Report
Wed 25 May 2016
SRR1294620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365614
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52901.4468811369367693No Hit
GGTATCAACGCAGAGTACTTTTTTT41751.1419146969207963No Hit
TATCAACGCAGAGTACTTTTTTTTT35250.9641315704540854No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18550.507365691685767No Hit
GTACTGGTTCACTATCGGTCAGTCA14440.3949520532583545No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10900.2981286274595612No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10030.2743330397632476No Hit
GGATACCACGTGTCCCGCCCTACTC9460.2587428271346283No Hit
ATACAGGGTGACAGCCCCGTACACA8970.2453407145240609No Hit
ACCCTGTATCGCGCGCCTTTCCAGA7010.19173226408179117No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6300.17231287642158125No Hit
GGTATCAACGCAGAGTACATGGGGG6000.16410750135388688No Hit
CCCATATTCAGACAGGATACCACGT5590.1528934887613713No Hit
ATCATTAACTGAATCCATAGGTTAA5280.14441460119142047No Hit
GTTCACTATCGGTCAGTCAGGAGTA5160.14113245116434273No Hit
ATCAACGCAGAGTACTTTTTTTTTT5150.14085893866208626No Hit
GTGGTATCAACGCAGAGTACTTTTT5030.1375767886350085No Hit
CTATCGGTCAGTCAGGAGTATTTAG4980.1362092261237261No Hit
GTATTTAGCCTTGGAGGATGGTCCC4660.1274568260515188No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4660.1274568260515188No Hit
TATTCAGACAGGATACCACGTGTCC4600.12581575103797993No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4430.12116603849961982No Hit
GCCTTGGAGGATGGTCCCCCCATAT4380.11979847598833744No Hit
GGTATCAACGCAGAGTACATGGGGA4320.11815740097479856No Hit
GGTTAATGAGGCGAACCGGGGGAAC4240.11596930095674673No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4110.1124136384274125No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4030.11022553840936068No Hit
CGGTACTGGTTCACTATCGGTCAGT3860.10557582587100056No Hit
TCACGGTACTGGTTCACTATCGGTC3730.10202016334166635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACT250.00593460619.0489164
GTCAATA350.002154567516.2985291
ATTAGAG350.002168428216.2828963
GGTATCA8100.015.37628651
ACTGTTC456.614524E-414.8158238
GTGGTAT1550.014.7212521
AACCCCG551.9886432E-413.78933713
TAGAGAG500.001466999413.3342415
GTTTTAT751.4593985E-512.6766331
TGGTATC1750.012.4835542
GTCTTGT701.0785417E-412.2238971
GTACTGG2900.012.1302271
CAACCCC550.003109009512.0656712
CGCGCCT1353.783498E-1011.93799112
GCAGTCA1001.9649087E-611.37620413
GCGCCTT1354.825779E-911.23575613
CGCGCGC1451.1496013E-911.16660510
TTGAAGA600.00574990711.1118674
TCATACA600.00574990711.1118674
ACTGCAC600.005868626311.0814153
Produced by FastQC (version 0.11.5)