FastQCFastQC Report
Wed 25 May 2016
SRR1294619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177609
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC145600.3485247183257217No Hit
CCCATGTACTCTGCGTTGATACCAC144610.3461549417382048No Hit
GTATCAACGCAGAGTACTTTTTTTT79810.19104229237346051No Hit
GAGTACATGGGAAGCAGTGGTATCA66080.15817660293244296No Hit
ATTCCATTCCATTCCATTCCATTCC61230.14656709136733476No Hit
CATGTACTCTGCGTTGATACCACTG52550.12578965623637828No Hit
GGTATCAACGCAGAGTACTTTTTTT46590.11152312243678142No Hit
GAATGGAATGGAATGGAATGGAATG45580.1091054715747692No Hit
TATCAACGCAGAGTACTTTTTTTTT45090.10793255184963456No Hit
GCGTTGATACCACTGCTTCCCATGT44900.10747774624192931No Hit
GTATCAACGCAGAGTACATGGGAAG44140.10565852381110821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT853.9617225E-612.290256
GTCTCGC1900.011.0008881
GGACCGT700.001496236210.8537276
CGCGTTG1201.5324185E-610.2880611
TAGACTG5300.010.2138895
GTTCGAT750.002656885410.12978211
TGTCGCC2600.09.86228110
TTACACC3300.09.4972394
TACACCG900.00111743059.4971255
GACCGTG800.00452988049.4970127
AGGTCGT1002.7660636E-49.4970126
ACTCGCG1304.2601314E-69.4970118
TCGCGTT1304.2601314E-69.49701110
GTCTAGG2950.09.3397371
TCTGTCG2750.09.3243398
TAGGACC2451.8189894E-129.3034184
TATACTG10750.09.2762615
CTGTCGC2800.09.1578339
CCCCGTA2600.09.13885615
TATCGCG1252.7667314E-59.1171317
Produced by FastQC (version 0.11.5)