##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294619_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4177609 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.003118769611994 31.0 30.0 33.0 25.0 34.0 2 30.09217856434147 31.0 30.0 34.0 25.0 34.0 3 30.09476808384892 31.0 30.0 34.0 25.0 34.0 4 33.72866010198657 35.0 35.0 37.0 28.0 37.0 5 34.063576557787 35.0 35.0 37.0 28.0 37.0 6 34.07270307010541 37.0 35.0 37.0 29.0 37.0 7 34.00720244522645 37.0 35.0 37.0 28.0 37.0 8 33.863568850028805 37.0 35.0 37.0 28.0 37.0 9 35.050986820451605 39.0 35.0 39.0 27.0 39.0 10 35.13915160561939 38.0 35.0 39.0 27.0 39.0 11 35.37005234333802 39.0 35.0 39.0 28.0 39.0 12 35.14266533799597 38.0 35.0 39.0 27.0 39.0 13 35.0039223393094 38.0 35.0 39.0 27.0 39.0 14 35.91046984052361 39.0 35.0 41.0 27.0 41.0 15 35.879423612884786 39.0 35.0 41.0 27.0 41.0 16 35.987309966059534 39.0 35.0 41.0 27.0 41.0 17 35.96514106514037 39.0 35.0 41.0 27.0 41.0 18 35.85656867361211 39.0 35.0 41.0 27.0 41.0 19 36.12765914665542 39.0 35.0 41.0 27.0 41.0 20 36.24634689364179 39.0 35.0 41.0 27.0 41.0 21 36.2713880116593 39.0 36.0 41.0 27.0 41.0 22 36.26005210157293 39.0 35.0 41.0 27.0 41.0 23 36.23010770036162 39.0 35.0 41.0 27.0 41.0 24 36.285191601224525 39.0 35.0 41.0 27.0 41.0 25 35.00234440322203 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9031532609796216 1101 2 0.9008296951399437 1101 3 1.160537073355048 1101 4 0.9394626915533806 1101 5 0.7241014373690149 1101 6 0.699066450149509 1101 7 0.7017866641516335 1101 8 0.895767538188295 1101 9 1.0768787662881607 1101 10 0.9695314666158907 1101 11 0.8684967694716832 1101 12 1.0533190320278791 1101 13 1.1155807062474707 1101 14 1.151061634838122 1101 15 1.2998819434243103 1101 16 1.1560063494778916 1101 17 1.2291882352115522 1101 18 1.2042766050143854 1101 19 1.082612507976755 1101 20 1.0107662251367984 1101 21 0.9075378626183905 1101 22 0.8837835257894682 1101 23 0.9139444041362026 1101 24 0.9414929379552888 1101 25 0.8655250685919071 1102 1 0.8109586513396785 1102 2 0.7832408818478136 1102 3 1.1422614411821783 1102 4 0.8881223193239052 1102 5 0.7223755572141428 1102 6 0.6350153796555418 1102 7 0.6161576989049493 1102 8 0.7349340222546843 1102 9 0.9935091915588856 1102 10 0.9658453175884532 1102 11 0.7769941901547668 1102 12 0.9917599110858859 1102 13 0.9908690942770804 1102 14 1.18215273040758 1102 15 1.236755020640281 1102 16 1.1543783258239984 1102 17 1.1564988048051603 1102 18 1.3134877882395486 1102 19 1.0033856355342508 1102 20 0.3671463365064582 1102 21 0.5574238638957567 1102 22 0.702181055868671 1102 23 0.7826257590783854 1102 24 0.8540606622566216 1102 25 0.8084542707699356 1103 1 0.6088344223445254 1103 2 0.6505386030946276 1103 3 0.8949573475218706 1103 4 0.6710208674181004 1103 5 0.5401195587417291 1103 6 0.5285036457623562 1103 7 0.40850895041413793 1103 8 0.5349039503307012 1103 9 0.6624658536772614 1103 10 0.6613318437752653 1103 11 0.5931749240340238 1103 12 0.8232542035117945 1103 13 0.6628128096800694 1103 14 0.6176361462201143 1103 15 0.8952316358966783 1103 16 0.7722331049103488 1103 17 0.8841565711437056 1103 18 1.0264877603928682 1103 19 0.7889926458355347 1103 20 -0.2014181474449046 1103 21 0.22823425687203525 1103 22 -0.3016777599912004 1103 23 0.27612337371220974 1103 24 0.42940947917069394 1103 25 0.5139718161649967 1104 1 0.3023578793081434 1104 2 0.26781328785304126 1104 3 0.6159881563568277 1104 4 0.4045229956371159 1104 5 0.29109248369404384 1104 6 0.1691763873027412 1104 7 0.19891862233346558 1104 8 0.32899449268577285 1104 9 0.5160111173440214 1104 10 0.5848134439854178 1104 11 0.25009153653206084 1104 12 0.48137840619004635 1104 13 0.28354372436111674 1104 14 0.647301868846256 1104 15 0.6482452731090191 1104 16 0.4685046217347377 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0.4006855590111158 2207 9 -1.1469149413623612 2207 10 -0.13786229364712455 2207 11 0.13375942411983033 2207 12 0.43376823853029123 2207 13 0.6560472309022316 2207 14 0.8451239250891334 2207 15 0.8547496520078681 2207 16 0.8536202843871692 2207 17 0.8182570027308032 2207 18 0.8562274462027872 2207 19 -0.28965611373779865 2207 20 0.22753909363017044 2207 21 -0.2710142151746311 2207 22 0.3024149819155255 2207 23 -0.50220792286499 2207 24 -1.5724551093669348 2207 25 -0.21331195532344793 2208 1 0.5275559710348539 2208 2 0.18066572278322823 2208 3 0.6797400200139876 2208 4 0.6251350383125995 2208 5 0.48160758424725003 2208 6 0.35672096211861515 2208 7 0.40261712290978124 2208 8 0.581053955495662 2208 9 -0.08681881378136325 2208 10 0.09268031553349232 2208 11 0.3144580003322517 2208 12 0.26435802628786575 2208 13 0.6005954299356446 2208 14 0.8833119668034328 2208 15 0.8178523345986335 2208 16 0.5769023918169367 2208 17 0.5614063815661225 2208 18 0.8885429574009507 2208 19 -1.3304277133875644 2208 20 -0.03626438418327638 2208 21 -0.09002508642655016 2208 22 0.26161437201113813 2208 23 0.45034852672420556 2208 24 0.5220418225138772 2208 25 0.5022557165094028 2209 1 0.21215841401003743 2209 2 -1.3601011918896262 2209 3 -0.15576828742201698 2209 4 0.15058317835020318 2209 5 0.23665337121667562 2209 6 0.2592971315554351 2209 7 0.2069081272602773 2209 8 0.41587840406619137 2209 9 -1.9322109961291645 2209 10 -0.29036324707436023 2209 11 0.040112166155005013 2209 12 0.43572850731830925 2209 13 0.43159042288176863 2209 14 0.679123933339433 2209 15 0.7982857938638332 2209 16 0.7190503121417819 2209 17 0.5188108375283207 2209 18 0.7415226546757836 2209 19 0.5013551034542658 2209 20 0.4107335783121613 2209 21 0.47425513356485993 2209 22 0.43597828980273334 2209 23 0.4473994343739278 2209 24 0.530350833747633 2209 25 0.5442589189123765 2210 1 0.17675717577524708 2210 2 0.19473386695644024 2210 3 0.15708872743955027 2210 4 0.31660949399924476 2210 5 0.0930237810958161 2210 6 0.18070683065870696 2210 7 0.10231628608328691 2210 8 0.21893278769989877 2210 9 0.33267491106473557 2210 10 -1.506907609758116 2210 11 -0.5200003888986444 2210 12 -2.361456127740034 2210 13 -0.7323031213946862 2210 14 -0.35170275114550265 2210 15 -0.693267970877173 2210 16 -0.0989485396697205 2210 17 0.13125729354958793 2210 18 0.40368558846591895 2210 19 0.2108441876587861 2210 20 0.26675596461748086 2210 21 0.22189895976259777 2210 22 0.1926697318381727 2210 23 0.13001484094022686 2210 24 0.13944104322181516 2210 25 0.26427686076201695 2211 1 -0.3731964824953877 2211 2 -0.15469495237925557 2211 3 -1.5030311694602503 2211 4 -0.6893202753709033 2211 5 -0.5554901515828234 2211 6 -0.48783983702875133 2211 7 -0.47754240354196753 2211 8 -0.08541567890505775 2211 9 0.13340361880908347 2211 10 0.08312137847259038 2211 11 -0.23380738226580178 2211 12 -0.3439938797195623 2211 13 -0.3470401973301591 2211 14 -0.8690470278549256 2211 15 -0.40147005097702504 2211 16 -0.17900402214570477 2211 17 -0.2862354622927157 2211 18 -0.0454780564183892 2211 19 -0.1015004602484737 2211 20 -0.1077027245437705 2211 21 -0.2230418457831007 2211 22 -0.32590506568501354 2211 23 -0.5849638035844933 2211 24 -0.4330576053860753 2211 25 -0.3162981246800669 2212 1 -0.41901636229150085 2212 2 -0.39926877277405737 2212 3 -2.546042866508415 2212 4 -1.5190312593328343 2212 5 -0.6824844397240497 2212 6 -0.49673056550353323 2212 7 -0.3889096811972337 2212 8 -0.4340199805305147 2212 9 -1.0097711897393822 2212 10 -0.3776757695520203 2212 11 -0.5231838219785345 2212 12 -0.12080000515873479 2212 13 -0.5498133237928187 2212 14 -0.14740121594162758 2212 15 -0.05714348455786222 2212 16 -1.275629402009379 2212 17 -1.5114729729367866 2212 18 -0.41665975748090744 2212 19 -0.21394709826325453 2212 20 -0.26667475625504977 2212 21 -0.18838828896820559 2212 22 -0.4804803574337413 2212 23 -0.18178856145161149 2212 24 -0.31397044346336145 2212 25 -0.14728353271687666 2213 1 -3.037223987518633 2213 2 -1.310598821877747 2213 3 -2.3781426287978746 2213 4 -1.0115364336056274 2213 5 -0.7943202054292797 2213 6 -0.6032890098846195 2213 7 -0.42740609184776446 2213 8 -0.18240441262830842 2213 9 -0.36228364330743545 2213 10 -0.13491136843380502 2213 11 -0.47718912695306415 2213 12 -0.13447869333406715 2213 13 -0.49238181528196634 2213 14 -0.1824230195604386 2213 15 -0.30179052428530184 2213 16 -1.5647542042214582 2213 17 -0.8709595939528185 2213 18 -0.2690592738197779 2213 19 -0.09276622614758168 2213 20 -0.08343887451498944 2213 21 -0.010427140557212056 2213 22 0.02624922051746381 2213 23 0.11758318357546926 2213 24 0.027369411745340244 2213 25 -0.009263199734242278 2214 1 -0.9287113475783926 2214 2 -0.6787932210448453 2214 3 -6.428326280368655 2214 4 -2.056087084585897 2214 5 -1.270197397974485 2214 6 -0.7006713643798435 2214 7 -0.5541697733331645 2214 8 -0.40001492508149283 2214 9 -0.19174595444273734 2214 10 -0.24373704515985395 2214 11 -0.758661204038539 2214 12 -0.43104608879158235 2214 13 -4.954315478732859 2214 14 -3.855383719486767 2214 15 -2.1174697127557067 2214 16 -2.9608342991028422 2214 17 -3.275150066535346 2214 18 -1.178752115612248 2214 19 -0.7796066514921307 2214 20 -0.3379326637920528 2214 21 -0.3297176170942464 2214 22 -0.34046978510225046 2214 23 -0.2762517862224598 2214 24 -0.1973427157051546 2214 25 0.15670259019680088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1654.0 3 236.0 4 221.0 5 442.0 6 59.0 7 81.0 8 238.0 9 738.0 10 1662.0 11 3361.0 12 6283.0 13 10071.0 14 14999.0 15 19967.0 16 24154.0 17 27302.0 18 28793.0 19 29475.0 20 30359.0 21 31452.0 22 32323.0 23 34019.0 24 36925.0 25 39748.0 26 43895.0 27 50162.0 28 57815.0 29 70389.0 30 86179.0 31 106779.0 32 138806.0 33 181559.0 34 242140.0 35 328537.0 36 460659.0 37 692184.0 38 1343943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.60581143135922 21.96381178216142 13.495101708675069 22.935275077804285 2 14.230026122730127 21.84700419683323 41.64155264866569 22.28141703177096 3 17.802195275472872 28.010959132466684 29.94346547715195 24.243380114908504 4 11.56069544133049 17.888193194220477 39.42246458571386 31.128646778735163 5 12.60440383925922 38.67057135528667 35.415889812292825 13.309134993161287 6 29.73584706796833 39.5601563239206 18.008223414333845 12.695773193777224 7 24.851312450567228 33.458495155049434 24.756459015890826 16.933733378492512 8 23.301565493654216 38.315516468713476 21.972552184119817 16.410365853512488 9 24.101332649861728 17.364163012768383 22.759173270830814 35.775331066539074 10 14.45934933838208 30.636998576686693 33.91576917125415 20.987882913677076 11 31.640452097133625 26.98688297478917 23.27962995253689 18.093034975540306 12 20.207249045922886 28.272107846342625 32.80224058719365 18.71840252054083 13 25.797233082577097 24.796771043439584 27.483352578976 21.92264329500732 14 19.724714715599458 23.445645839426337 29.19904792222223 27.630591522751974 15 22.704942673419943 30.717178092659108 24.617241928587596 21.960637305333357 16 21.06316554434216 32.05563829741368 26.443329119326958 20.437867038917204 17 19.43531633480908 30.788920179561675 29.632873937712002 20.142889547917246 18 19.791465658856957 30.481697212662407 30.155066763158406 19.57177036532223 19 20.314520021606782 29.142735318107178 30.42752445435088 20.11522020593516 20 21.868083646794837 28.843983359553597 29.118146402969376 20.169786590682193 21 21.94433825692143 29.66246259883823 28.449529629193172 19.943669515047173 22 20.358368335387347 29.32737370216935 29.88446028569341 20.429797676749892 23 20.189571257373725 29.85228714552999 29.46958516945407 20.488556427642212 24 20.373180164003227 28.811858134717212 29.555674477165457 21.25928722411411 25 21.195131819215117 28.675814487501732 28.4703825666486 21.65867112663455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3998.0 1 3998.0 2 3896.5 3 3795.0 4 3795.0 5 3795.0 6 6000.0 7 8205.0 8 8205.0 9 8205.0 10 13247.5 11 18290.0 12 18290.0 13 18290.0 14 30431.0 15 42572.0 16 42572.0 17 42572.0 18 68310.5 19 94049.0 20 94049.0 21 94049.0 22 133593.5 23 173138.0 24 173138.0 25 173138.0 26 224980.5 27 276823.0 28 276823.0 29 276823.0 30 330567.5 31 384312.0 32 384312.0 33 384312.0 34 428178.5 35 472045.0 36 472045.0 37 472045.0 38 498639.0 39 525233.0 40 525233.0 41 525233.0 42 513698.5 43 502164.0 44 502164.0 45 502164.0 46 502785.5 47 503407.0 48 503407.0 49 503407.0 50 458794.0 51 414181.0 52 414181.0 53 414181.0 54 358094.5 55 302008.0 56 302008.0 57 302008.0 58 255302.5 59 208597.0 60 208597.0 61 208597.0 62 170509.0 63 132421.0 64 132421.0 65 132421.0 66 99544.5 67 66668.0 68 66668.0 69 66668.0 70 47728.0 71 28788.0 72 28788.0 73 28788.0 74 20073.5 75 11359.0 76 11359.0 77 11359.0 78 7547.5 79 3736.0 80 3736.0 81 3736.0 82 2486.0 83 1236.0 84 1236.0 85 1236.0 86 808.0 87 380.0 88 380.0 89 380.0 90 258.0 91 136.0 92 136.0 93 136.0 94 84.0 95 32.0 96 32.0 97 32.0 98 34.0 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10333662149808658 2 0.05548628414004279 3 0.05890929476645612 4 0.0537628102582123 5 0.05065098241601835 6 0.070470932057069 7 0.0657553160192828 8 0.06300254523580355 9 0.06807721833230443 10 0.0680532811950568 11 0.06168600268718303 12 0.06300254523580355 13 0.06314616805928941 14 0.06558775605854927 15 0.06807721833230443 16 0.06326585374552765 17 0.06644949299946452 18 0.14175572678055798 19 0.07166778891945129 20 0.06513295045084401 21 0.0623562425301171 22 0.06831658970478088 23 0.06750272703836094 24 0.07317582856605297 25 0.07157204037046071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4177609.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.79197888323735 #Duplication Level Percentage of deduplicated Percentage of total 1 89.89107945724442 70.82696034386612 2 7.638555045034799 12.03713735613656 3 1.3071287989231928 3.089737941672828 4 0.3971351772299592 1.2516426599237467 5 0.1912148889067608 0.7533099744451036 6 0.11256582592836267 0.5321570507713035 7 0.08116668376144914 0.44766985430681533 8 0.05273890781753296 0.33243223288672463 9 0.04268600471736095 0.3026983304070066 >10 0.2494803073796596 3.603874849856882 >50 0.02049756892787796 1.1014988913213175 >100 0.01336076956119271 1.984209971258739 >500 0.0010420411954515359 0.5378809481570054 >1k 0.001164633821314988 1.8709190622467065 >5k 1.2259303382261062E-4 0.6270607064039988 >10k+ 6.129651691130531E-5 0.7008098263392171 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 14560 0.3485247183257217 No Hit CCCATGTACTCTGCGTTGATACCAC 14461 0.3461549417382048 No Hit GTATCAACGCAGAGTACTTTTTTTT 7981 0.19104229237346051 No Hit GAGTACATGGGAAGCAGTGGTATCA 6608 0.15817660293244296 No Hit ATTCCATTCCATTCCATTCCATTCC 6123 0.14656709136733476 No Hit CATGTACTCTGCGTTGATACCACTG 5255 0.12578965623637828 No Hit GGTATCAACGCAGAGTACTTTTTTT 4659 0.11152312243678142 No Hit GAATGGAATGGAATGGAATGGAATG 4558 0.1091054715747692 No Hit TATCAACGCAGAGTACTTTTTTTTT 4509 0.10793255184963456 No Hit GCGTTGATACCACTGCTTCCCATGT 4490 0.10747774624192931 No Hit GTATCAACGCAGAGTACATGGGAAG 4414 0.10565852381110821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.787427449529144E-5 0.0 10 0.0 0.0 0.0 4.787427449529144E-5 0.0 11 0.0 0.0 0.0 4.787427449529144E-5 0.0 12 0.0 0.0 0.0 4.787427449529144E-5 0.0 13 0.0 0.0 0.0 9.574854899058288E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 85 3.9617225E-6 12.29025 6 GTCTCGC 190 0.0 11.000888 1 GGACCGT 70 0.0014962362 10.853727 6 CGCGTTG 120 1.5324185E-6 10.28806 11 TAGACTG 530 0.0 10.213889 5 GTTCGAT 75 0.0026568854 10.129782 11 TGTCGCC 260 0.0 9.862281 10 TTACACC 330 0.0 9.497239 4 TACACCG 90 0.0011174305 9.497125 5 GACCGTG 80 0.0045298804 9.497012 7 AGGTCGT 100 2.7660636E-4 9.497012 6 ACTCGCG 130 4.2601314E-6 9.497011 8 TCGCGTT 130 4.2601314E-6 9.497011 10 GTCTAGG 295 0.0 9.339737 1 TCTGTCG 275 0.0 9.324339 8 TAGGACC 245 1.8189894E-12 9.303418 4 TATACTG 1075 0.0 9.276261 5 CTGTCGC 280 0.0 9.157833 9 CCCCGTA 260 0.0 9.138856 15 TATCGCG 125 2.7667314E-5 9.117131 7 >>END_MODULE