FastQCFastQC Report
Wed 25 May 2016
SRR1294619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177609
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT181570.4346266010055034No Hit
CCCATGTACTCTGCGTTGATACCAC162110.3880449319215848No Hit
GTACATGGGAAGCAGTGGTATCAAC144580.3460831303264618No Hit
GGTATCAACGCAGAGTACTTTTTTT116890.27980119728773084No Hit
TATCAACGCAGAGTACTTTTTTTTT111300.2664203375662969No Hit
GAGTACATGGGAAGCAGTGGTATCA69860.16722484081205302No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65910.15776967159923297No Hit
ATTCCATTCCATTCCATTCCATTCC60080.1438143205838555No Hit
CATGTACTCTGCGTTGATACCACTG58990.14120517262386212No Hit
GAATGGAATGGAATGGAATGGAATG50950.12195971427675495No Hit
GCGTTGATACCACTGCTTCCCATGT50050.11980537192446686No Hit
GTATCAACGCAGAGTACATGGGAAG43850.1049643468309265No Hit
ACGCAGAGTACATGGGAAGCAGTGG43550.10424623271349713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG602.5199435E-514.2799365
CGCGGTG550.003110731312.06961318
GTTCCGA752.0984399E-411.38646211
TCTGTCG2150.011.0697178
CGACCAT750.002603448710.1546216
ATGCCGA750.00267540910.121311
TACACCG951.6108534E-410.0210085
AGCCCGT800.0044405969.5199586
AACCGCT900.00109315589.5199577
CTGTCGC2600.09.51959
TCAGGAC4050.09.3822483
AAGGCCG1356.674074E-69.1673665
TGTCGCC2700.09.16692510
GTCGCCC2106.8394E-109.03687611
AAGACCG950.00179028239.0189075
TACGACC950.00179028239.0189074
ACGGTGC950.00179028239.0189078
TATACTG10200.08.9599595
CCCGTAC2451.8189894E-118.9026616
TCGCCCA2356.91216E-118.87795312
Produced by FastQC (version 0.11.5)