##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294619_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4177609 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.191021227692683 31.0 31.0 34.0 28.0 34.0 2 31.419026050547096 31.0 31.0 34.0 28.0 34.0 3 31.542086155023124 31.0 31.0 34.0 28.0 34.0 4 35.15624966338401 37.0 35.0 37.0 32.0 37.0 5 35.251728201466435 37.0 35.0 37.0 32.0 37.0 6 34.791427584534595 37.0 35.0 37.0 32.0 37.0 7 34.636758011580305 37.0 35.0 37.0 31.0 37.0 8 34.67239035534441 37.0 35.0 37.0 31.0 37.0 9 36.14831905044249 39.0 35.0 39.0 30.0 39.0 10 35.95164434967466 39.0 35.0 39.0 30.0 39.0 11 36.07333836172796 39.0 35.0 39.0 30.0 39.0 12 36.030941143606306 39.0 35.0 39.0 30.0 39.0 13 35.89173424319988 39.0 35.0 39.0 30.0 39.0 14 36.98037058997144 40.0 36.0 41.0 30.0 41.0 15 37.01916957762203 40.0 36.0 41.0 30.0 41.0 16 37.060393636647184 40.0 36.0 41.0 30.0 41.0 17 37.09244426656492 40.0 36.0 41.0 30.0 41.0 18 37.099733603599574 40.0 36.0 41.0 30.0 41.0 19 37.116806287998706 40.0 37.0 41.0 30.0 41.0 20 37.1846661571248 40.0 37.0 41.0 30.0 41.0 21 37.0239818996943 40.0 36.0 41.0 30.0 41.0 22 37.018235071783884 40.0 36.0 41.0 30.0 41.0 23 37.026947950370655 40.0 36.0 41.0 30.0 41.0 24 36.957694700485376 40.0 36.0 41.0 30.0 41.0 25 35.846786044361735 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4378334828984265 1101 2 0.4454063206447678 1101 3 0.32237829775206706 1101 4 0.3044346130967597 1101 5 0.3620163188298804 1101 6 0.4004233629671816 1101 7 0.5573787827504759 1101 8 0.4657403266283211 1101 9 0.6994474732576705 1101 10 0.6832350703779042 1101 11 0.6965112485549056 1101 12 0.7351985259555107 1101 13 0.6932035832484829 1101 14 0.9484353061317634 1101 15 0.908212620677098 1101 16 0.9368816174665966 1101 17 0.9300723198531387 1101 18 0.9069566323700258 1101 19 0.9821812304453985 1101 20 0.8783370994490696 1101 21 1.2361956048358707 1101 22 1.1730972030353968 1101 23 1.0912431876576747 1101 24 1.1713118279190127 1101 25 1.0809951459363276 1102 1 0.3865491052818619 1102 2 0.4163796912472755 1102 3 0.3266001653802988 1102 4 0.2556684472942834 1102 5 0.28559159032173653 1102 6 0.3748222631635798 1102 7 0.4621337127843361 1102 8 0.4283623136528405 1102 9 0.5637905706424249 1102 10 0.6449704812890857 1102 11 0.6127647433285404 1102 12 0.6748060646033096 1102 13 0.722051234935364 1102 14 0.8766769793858131 1102 15 0.8671252248530976 1102 16 0.8598912014099085 1102 17 0.7959844651572325 1102 18 0.7709296605694149 1102 19 0.8908602156324719 1102 20 0.7677563195384352 1102 21 1.1144007601384232 1102 22 0.9683207079776537 1102 23 0.9556759868349971 1102 24 0.9971089908880657 1102 25 1.0255365355453279 1103 1 0.30598717924661045 1103 2 0.287856216499236 1103 3 0.25587190766726664 1103 4 0.20162737245236428 1103 5 0.24158582357345182 1103 6 0.30035167323174505 1103 7 0.31263765060271 1103 8 0.362305273714739 1103 9 0.3208342879350141 1103 10 0.5131395300068462 1103 11 0.4480401544150965 1103 12 0.5102751592680832 1103 13 0.6335975111385892 1103 14 0.7706849465187773 1103 15 0.7622448835125226 1103 16 0.731551651471861 1103 17 0.7193934254220125 1103 18 0.7206501868517066 1103 19 0.8045168720820612 1103 20 0.7438047593289454 1103 21 0.9302192385960382 1103 22 0.896426557171992 1103 23 0.8853664859468822 1103 24 0.9048953442265883 1103 25 0.9080910653044754 1104 1 0.18947938517051455 1104 2 0.1671007055752156 1104 3 0.0889834196152357 1104 4 0.10554606760817364 1104 5 0.1537518950472503 1104 6 0.18764399093587514 1104 7 0.23466720346194592 1104 8 0.15354446750669126 1104 9 -0.07579358422336924 1104 10 0.39693652760786335 1104 11 0.4308892174673886 1104 12 0.4933986450085328 1104 13 -0.04465737409425685 1104 14 0.34483347890053295 1104 15 0.502975979445381 1104 16 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2207 4 0.19246972431920994 2207 5 0.1341917362786731 2207 6 0.3079953319817861 2207 7 0.3170041037734421 2207 8 0.31453709336371816 2207 9 0.3274597369800176 2207 10 0.4468459850600368 2207 11 0.40179914006895245 2207 12 0.3431868456570015 2207 13 0.43169892483709305 2207 14 0.4747855884564558 2207 15 0.44295033696419495 2207 16 0.5799915186586446 2207 17 0.559144372712673 2207 18 0.5985414823070414 2207 19 0.6725682912710766 2207 20 0.5282095723641191 2207 21 0.8965349505721392 2207 22 0.7678645612706205 2207 23 0.6609566143962198 2207 24 0.8299001632751768 2207 25 0.7347966222676519 2208 1 0.2017658794135322 2208 2 0.18485252707308675 2208 3 0.20401165568755886 2208 4 0.14092835942961557 2208 5 0.10830878147666567 2208 6 0.27553588042184174 2208 7 0.3248981695170343 2208 8 0.20736833320336245 2208 9 0.3502964041137844 2208 10 0.4262332218276583 2208 11 0.1064529234920002 2208 12 -8.05518022751528E-4 2208 13 0.08904754365436673 2208 14 -0.2937599550769576 2208 15 0.23006169816316913 2208 16 0.32842152319299345 2208 17 0.31427688510127183 2208 18 0.33753214340968185 2208 19 0.6192331982916599 2208 20 0.4522569992789869 2208 21 0.7194057747143816 2208 22 0.7292184303393086 2208 23 0.7239473129042295 2208 24 0.7438374250488451 2208 25 0.6871316208195992 2209 1 0.010139916048007791 2209 2 -0.02559323507074751 2209 3 0.10394842954695704 2209 4 -0.5042427729609926 2209 5 -0.04453790356858889 2209 6 0.16254443124803686 2209 7 0.0931797479848342 2209 8 0.10000358348149518 2209 9 0.237325859962219 2209 10 0.25432766744165747 2209 11 0.34062206332454537 2209 12 0.3035784667699275 2209 13 0.3972239487172473 2209 14 0.372620645566478 2209 15 -0.3034020126855097 2209 16 -0.28468776001607665 2209 17 0.15966755523044895 2209 18 -0.21256871667166166 2209 19 0.27926200465619644 2209 20 0.30180368024265647 2209 21 0.5540162863560525 2209 22 0.565987354022603 2209 23 0.4886509541096373 2209 24 0.5252823065353667 2209 25 0.5394642005406496 2210 1 -0.1379241729804157 2210 2 -0.09072814581509903 2210 3 -0.026567895766749672 2210 4 -0.012593639480989793 2210 5 -0.06809360292857747 2210 6 -0.5518337929413875 2210 7 -0.17442102957021177 2210 8 -0.29435296331350713 2210 9 -0.015017576474960492 2210 10 0.109461864781224 2210 11 0.06575281441340053 2210 12 0.11988006117346828 2210 13 0.18898847682666542 2210 14 0.1794255161551277 2210 15 -0.011094089201819202 2210 16 0.06979177607981768 2210 17 -0.8034026244211674 2210 18 -1.424853083753817 2210 19 -0.3482199348274122 2210 20 -0.1803864780854596 2210 21 -0.31885573809294954 2210 22 -0.2968052437566797 2210 23 -0.13333824595294175 2210 24 0.16942764057311877 2210 25 0.2621543629413523 2211 1 -0.2724933050966847 2211 2 -0.23635630534664642 2211 3 -0.20941867191821828 2211 4 -0.19126548203743 2211 5 -0.17419499329646015 2211 6 -0.16664418044872065 2211 7 -0.22791371117295256 2211 8 -0.22656788917834092 2211 9 -0.22705511617572682 2211 10 -0.09056232107753459 2211 11 -0.4350119111206041 2211 12 -0.30465733352814794 2211 13 -0.2236335254608477 2211 14 -0.4137175318894748 2211 15 -0.3680063148344246 2211 16 -0.3532789231873039 2211 17 -0.5021331278350232 2211 18 -0.5538879461902155 2211 19 -0.3132084972942124 2211 20 -0.5704002704049174 2211 21 -0.22703960629915798 2211 22 -0.22232903869772258 2211 23 -0.8106490104580715 2211 24 -0.26952742592929724 2211 25 -0.9264082778528291 2212 1 -0.2360296411334737 2212 2 -0.37379525093340504 2212 3 -0.2529842361020762 2212 4 -0.14948652891467873 2212 5 -0.10746448892076899 2212 6 -0.16740410796128913 2212 7 -0.2548309118238734 2212 8 -0.4361371433872776 2212 9 -0.23096204124556863 2212 10 -0.6562157931463801 2212 11 -0.3445086631175869 2212 12 -0.40221131694330126 2212 13 -0.2546293604637455 2212 14 -0.2973235204292948 2212 15 -0.21795729947137232 2212 16 -0.4368199387331515 2212 17 -0.62705390937586 2212 18 -0.43468090187234765 2212 19 -0.5241458211890375 2212 20 -0.7961821019369211 2212 21 -0.19091762101960086 2212 22 -0.4944292489245754 2212 23 -0.3812283309697051 2212 24 -0.4821824585325061 2212 25 -0.25242574157369546 2213 1 -0.12117410054196043 2213 2 -0.20735338039383322 2213 3 -0.15953416246482988 2213 4 -0.016515741739716816 2213 5 -0.061230228623514904 2213 6 -0.26608453560479006 2213 7 -0.3352977650466471 2213 8 -0.3147751914932826 2213 9 -0.3187291333195148 2213 10 -0.20723607904986352 2213 11 -0.05284856828098583 2213 12 0.07161398051358248 2213 13 0.13108997731816174 2213 14 -0.6110992632122318 2213 15 -0.26755194356126566 2213 16 -0.26062167824604643 2213 17 -0.2676353590202112 2213 18 -0.2178005570734456 2213 19 -0.2385211704785064 2213 20 -0.40346848383179434 2213 21 -0.04635635415831274 2213 22 -0.2789407023124113 2213 23 -0.5154483848993721 2213 24 -0.6879979102976321 2213 25 -0.4391770152307046 2214 1 -0.2323945419105371 2214 2 -0.1075250521598079 2214 3 -0.086353771860054 2214 4 0.0018589526589494199 2214 5 -0.12433060210763358 2214 6 4.620671651522912E-4 2214 7 -0.017913057790870823 2214 8 -0.028735003817430993 2214 9 -0.021812877970553757 2214 10 -0.1380262377316015 2214 11 0.021771924462846926 2214 12 -0.1989778417825434 2214 13 -0.26506345855964497 2214 14 -0.14442315891847812 2214 15 0.01989554717640374 2214 16 -0.3208281894776519 2214 17 -0.30971338493446865 2214 18 -0.1263983682750478 2214 19 -0.3766631077375635 2214 20 -0.33904926842426875 2214 21 -0.4630810589084575 2214 22 -0.8840047203289743 2214 23 -1.0541182643469753 2214 24 -0.5009045631933446 2214 25 -0.7269872896828815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 40.0 10 104.0 11 280.0 12 697.0 13 1819.0 14 3783.0 15 6611.0 16 9728.0 17 12648.0 18 14981.0 19 16694.0 20 18488.0 21 19993.0 22 22159.0 23 24681.0 24 27714.0 25 31090.0 26 35137.0 27 40023.0 28 46615.0 29 58245.0 30 74122.0 31 95884.0 32 127071.0 33 170388.0 34 232248.0 35 321413.0 36 455898.0 37 719840.0 38 1589211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.518119668113584 22.06331125421591 12.661915391390774 24.756653686279734 2 14.050716570172078 22.32185922617459 40.120461249485054 23.50696295416828 3 17.361658065861473 27.711195108446844 30.304020287194188 24.62312653849749 4 11.556812457985325 18.019938177381885 39.3012684361827 31.12198092845009 5 12.827814108124635 38.3048539221571 35.32375163498532 13.54358033473294 6 30.071231543934257 39.83560772807429 17.493479898168147 12.599680829823306 7 24.928161229138503 33.75251453654715 24.278272345197685 17.04105188911666 8 23.40408059484873 38.361202003546744 21.61407089123162 16.62064651037291 9 24.007271158734405 16.816517422319652 22.77996390751534 36.3962475114306 10 14.2646437587678 30.53035250943522 33.95668520310059 21.248318528696387 11 31.860476368641244 26.55171439151832 23.127458480983915 18.46035075885652 12 20.247326841648057 28.089245631828614 32.748748129585074 18.914679396938254 13 26.074322913973734 24.422013745664984 27.48436425820709 22.01929908215419 14 19.682891600411914 23.103618809248072 29.359875448886218 27.853614141453797 15 22.737369989587197 30.346471017701766 24.78179794377087 22.134361048940168 16 20.964911944741534 31.746738082403215 26.561445268627665 20.726904704227593 17 19.228730890978763 30.606174633905336 29.813544018490962 20.35155045662494 18 19.601641519017885 30.322621106790177 30.45664561946422 19.619091754727723 19 20.16741247217891 28.84275751594342 30.876108834144887 20.113721177732785 20 21.594698779491786 28.433714636427965 29.776709329079537 20.194877255000712 21 21.778825588573724 29.39264874393162 28.838215443847055 19.990310223647604 22 20.12387369326156 29.04049114765573 30.36724763080909 20.468387528273617 23 19.93416318739794 29.487107675647415 30.097529635422426 20.481199501532217 24 20.149803392323218 28.569116927888654 30.141332039451274 21.139747640336854 25 21.01851913912478 28.32874847157976 28.99847660667248 21.654255782622975 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2329.0 1 2329.0 2 2538.5 3 2748.0 4 2748.0 5 2748.0 6 5596.0 7 8444.0 8 8444.0 9 8444.0 10 12722.0 11 17000.0 12 17000.0 13 17000.0 14 27312.0 15 37624.0 16 37624.0 17 37624.0 18 62514.5 19 87405.0 20 87405.0 21 87405.0 22 128092.0 23 168779.0 24 168779.0 25 168779.0 26 221821.0 27 274863.0 28 274863.0 29 274863.0 30 329732.0 31 384601.0 32 384601.0 33 384601.0 34 428678.0 35 472755.0 36 472755.0 37 472755.0 38 498980.5 39 525206.0 40 525206.0 41 525206.0 42 514481.0 43 503756.0 44 503756.0 45 503756.0 46 506440.5 47 509125.0 48 509125.0 49 509125.0 50 465023.0 51 420921.0 52 420921.0 53 420921.0 54 363691.5 55 306462.0 56 306462.0 57 306462.0 58 259197.0 59 211932.0 60 211932.0 61 211932.0 62 172990.5 63 134049.0 64 134049.0 65 134049.0 66 100342.5 67 66636.0 68 66636.0 69 66636.0 70 47403.0 71 28170.0 72 28170.0 73 28170.0 74 19300.0 75 10430.0 76 10430.0 77 10430.0 78 6764.0 79 3098.0 80 3098.0 81 3098.0 82 2029.0 83 960.0 84 960.0 85 960.0 86 593.0 87 226.0 88 226.0 89 226.0 90 148.5 91 71.0 92 71.0 93 71.0 94 42.0 95 13.0 96 13.0 97 13.0 98 9.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09893218824451977 2 0.0 3 0.13210906046975673 4 0.011896757212079924 5 0.023123274581225768 6 0.012614871329509296 7 0.02142373783664292 8 0.019532703994078908 9 1.9149709798116577E-4 10 0.384789481255905 11 0.06642555586221688 12 0.0014362282348587432 13 2.393713724764572E-5 14 0.001125045450639349 15 0.0014122910976110976 16 1.1968568623822863E-4 17 2.393713724764572E-5 18 3.590570587146858E-4 19 5.505541566958516E-4 20 1.1968568623822863E-4 21 2.3937137247645725E-4 22 0.0030639535676986523 23 1.6755996073352007E-4 24 0.0 25 0.002226153764031052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4177609.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.09698997269888 #Duplication Level Percentage of deduplicated Percentage of total 1 89.31745589514408 68.86107001534899 2 8.175339301093471 12.60588104239629 3 1.307367506763576 3.0238229867875126 4 0.41813881571703065 1.2894897633012836 5 0.1899773628377688 0.732334141887162 6 0.10861369073589341 0.5024273175337796 7 0.08164914695944549 0.44064324250882503 8 0.05854550312810551 0.361094565409133 9 0.042901440177735665 0.29768147128775047 >10 0.26095818827887834 3.7432435517534066 >50 0.021715666795503748 1.1488777781123916 >100 0.014778264359277982 2.1562603321859872 >500 0.0010299293562534054 0.5209413123624346 >1k 0.001185978955370606 1.736093709451254 >5k 1.872598350585032E-4 0.856220406607129 >10k+ 1.5604986254875265E-4 1.723918363066765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 18157 0.4346266010055034 No Hit CCCATGTACTCTGCGTTGATACCAC 16211 0.3880449319215848 No Hit GTACATGGGAAGCAGTGGTATCAAC 14458 0.3460831303264618 No Hit GGTATCAACGCAGAGTACTTTTTTT 11689 0.27980119728773084 No Hit TATCAACGCAGAGTACTTTTTTTTT 11130 0.2664203375662969 No Hit GAGTACATGGGAAGCAGTGGTATCA 6986 0.16722484081205302 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6591 0.15776967159923297 No Hit ATTCCATTCCATTCCATTCCATTCC 6008 0.1438143205838555 No Hit CATGTACTCTGCGTTGATACCACTG 5899 0.14120517262386212 No Hit GAATGGAATGGAATGGAATGGAATG 5095 0.12195971427675495 No Hit GCGTTGATACCACTGCTTCCCATGT 5005 0.11980537192446686 No Hit GTATCAACGCAGAGTACATGGGAAG 4385 0.1049643468309265 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4355 0.10424623271349713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.393713724764572E-5 0.0 4 0.0 0.0 0.0 7.181141174293717E-5 0.0 5 0.0 0.0 0.0 7.181141174293717E-5 0.0 6 0.0 0.0 0.0 7.181141174293717E-5 0.0 7 0.0 0.0 0.0 7.181141174293717E-5 0.0 8 0.0 0.0 0.0 9.574854899058288E-5 0.0 9 2.393713724764572E-5 0.0 0.0 1.9149709798116577E-4 0.0 10 2.393713724764572E-5 0.0 0.0 2.154342352288115E-4 0.0 11 2.393713724764572E-5 0.0 0.0 2.6330850972410295E-4 0.0 12 2.393713724764572E-5 0.0 0.0 2.6330850972410295E-4 0.0 13 2.393713724764572E-5 0.0 0.0 2.6330850972410295E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATATCG 60 2.5199435E-5 14.279936 5 CGCGGTG 55 0.0031107313 12.069613 18 GTTCCGA 75 2.0984399E-4 11.386462 11 TCTGTCG 215 0.0 11.069717 8 CGACCAT 75 0.0026034487 10.154621 6 ATGCCGA 75 0.002675409 10.1213 11 TACACCG 95 1.6108534E-4 10.021008 5 AGCCCGT 80 0.004440596 9.519958 6 AACCGCT 90 0.0010931558 9.519957 7 CTGTCGC 260 0.0 9.5195 9 TCAGGAC 405 0.0 9.382248 3 AAGGCCG 135 6.674074E-6 9.167366 5 TGTCGCC 270 0.0 9.166925 10 GTCGCCC 210 6.8394E-10 9.036876 11 AAGACCG 95 0.0017902823 9.018907 5 TACGACC 95 0.0017902823 9.018907 4 ACGGTGC 95 0.0017902823 9.018907 8 TATACTG 1020 0.0 8.959959 5 CCCGTAC 245 1.8189894E-11 8.90266 16 TCGCCCA 235 6.91216E-11 8.877953 12 >>END_MODULE