FastQCFastQC Report
Wed 25 May 2016
SRR1294618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032980
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC764392.5202606017843836No Hit
CCCATGTACTCTGCGTTGATACCAC733722.419138932666882No Hit
GAGTACATGGGAAGCAGTGGTATCA349661.1528595638612849No Hit
CATGTACTCTGCGTTGATACCACTG270340.8913345950187603No Hit
GCGTTGATACCACTGCTTCCCATGT238540.7864872171923323No Hit
GTATCAACGCAGAGTACTTTTTTTT233680.7704633726565951No Hit
GTATCAACGCAGAGTACATGGGAAG214780.7081484216842842No Hit
ACGCAGAGTACATGGGAAGCAGTGG185390.6112470243786639No Hit
TATCAACGCAGAGTACATGGGAAGC177940.586683723598573No Hit
ACTCTGCGTTGATACCACTGCTTCC160690.5298089667587653No Hit
GGTATCAACGCAGAGTACATGGGAA149260.4921232583136058No Hit
GGTATCAACGCAGAGTACTTTTTTT145210.4787700545338248No Hit
TATCAACGCAGAGTACTTTTTTTTT138590.45694333625675077No Hit
GCTTCCCATGTACTCTGCGTTGATA125190.4127623657261176No Hit
GTACTGGTTCACTATCGGTCAGTCA120790.39825518137277527No Hit
GTGGTATCAACGCAGAGTACATGGG108630.3581625991599021No Hit
CAGTGGTATCAACGCAGAGTACATG95450.314706987846936No Hit
ATACCACTGCTTCCCATGTACTCTG87770.2893853569756477No Hit
GTACTCTGCGTTGATACCACTGCTT82890.27329557069284993No Hit
GCAGTGGTATCAACGCAGAGTACAT76800.25321630871288303No Hit
GGGAAGCAGTGGTATCAACGCAGAG76750.2530514543452314No Hit
CATGGGAAGCAGTGGTATCAACGCA74640.24609460003033318No Hit
GTTAATGATAGTGTGTCGAAACACA72140.23785188164775237No Hit
GGATACCACGTGTCCCGCCCTACTC71560.23593957098299365No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70590.23274139625055226No Hit
CTGCTTCCCATGTACTCTGCGTTGA70570.23267545450349159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA67110.22126753226199974No Hit
ACATGGGAAGCAGTGGTATCAACGC66420.21899254198840742No Hit
TACCACTGCTTCCCATGTACTCTGC66170.21816827015014936No Hit
GCAGAGTACATGGGAAGCAGTGGTA65970.2175088526795429No Hit
CCATGTACTCTGCGTTGATACCACT60420.19921001787021345No Hit
GATACCACTGCTTCCCATGTACTCT58870.19409953247301334No Hit
GTTGATACCACTGCTTCCCATGTAC58800.1938687363583011No Hit
GTACATGGTAAGCAGTGGTATCAAC57780.1905057072582081No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57320.18898904707581324No Hit
GTACATGGGGGTTAAGCGACTAAGC56700.18694485291693316No Hit
AAGCAGTGGTATCAACGCAGAGTAC55470.18288943547270342No Hit
ACTTAGATGTTTCAGTTCCCCCGGT55350.18249378499033952No Hit
CTTCCCATGTACTCTGCGTTGATAC54990.1813068335432479No Hit
ATACAGGGTGACAGCCCCGTACACA54920.18107603742853565No Hit
CCACTGCTTCCCATGTACTCTGCGT53740.1771854743519575No Hit
ATGGGAAGCAGTGGTATCAACGCAG53260.17560287242250194No Hit
GTATCAACGCAGAGTACATGGGGGT51730.1705583287723625No Hit
AGCGTACACGGTGGATGCCCTGGCA50590.16679964918990564No Hit
GGTATCAACGCAGAGTACATGGGGG47130.15539172694841377No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46820.15436962986897373No Hit
TCATTAACCTATGGATTCAGTTAAT45670.15057797941298656No Hit
GTTCACTATCGGTCAGTCAGGAGTA44380.14632473672757487No Hit
TTCCCATGTACTCTGCGTTGATACC43770.14431351344222515No Hit
ACCCTGTATCGCGCGCCTTTCCAGA43740.14421460082163418No Hit
ATCATTAACTGAATCCATAGGTTAA43340.14289576588042124No Hit
GTGGATGCCCTGGCAGTCAGAGGCG43050.14193961054804186No Hit
ACCATGTACTCTGCGTTGATACCAC40800.13452116400371913No Hit
CTATCGGTCAGTCAGGAGTATTTAG40670.1340925426478249No Hit
GTATTTAGCCTTGGAGGATGGTCCC40610.13389471740664297No Hit
GTACTTAGATGTTTCAGTTCCCCCG39500.1302349504447771No Hit
ATCAACGCAGAGTACATGGGAAGCA38680.12753133881529058No Hit
TGATACCACTGCTTCCCATGTACTC38590.12723460095351766No Hit
TATTCAGACAGGATACCACGTGTCC37910.12499258155345568No Hit
GTCAGGAGTATTTAGCCTTGGAGGA35150.11589262045908644No Hit
CCCATATTCAGACAGGATACCACGT34950.11523320298847997No Hit
CCTATGGATTCAGTTAATGATAGTG32710.10784772731768756No Hit
GCCTTGGAGGATGGTCCCCCCATAT31110.10257238755283582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGC400.0052906814.2459187
GTACAAA7150.013.1535491
GAAATCG4050.013.14157716
ATCGCCG4000.012.82365219
AATCGCC3950.012.75433318
AGTACGC751.4812804E-512.66533619
AAATCGC4050.012.43797417
AGGGTGA10750.011.7503165
TCTATAC1950.011.6889583
GTACATA8500.011.5115041
CTAGTAC752.0805842E-411.3967343
ACAGGGT12550.011.1243023
CCTAGAC951.3655956E-510.9968483
CCGTACA14800.010.7888817
TCTGTCG1502.1464075E-910.7632278
CCGGTGA803.7576118E-410.69219816
CAGGGTG12200.010.5873054
CCCGTAC15250.010.53249616
CCCCGTA15100.010.51106715
TACAGGG15650.010.4379032
Produced by FastQC (version 0.11.5)