FastQCFastQC Report
Wed 25 May 2016
SRR1294618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032980
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC821612.708919940124894No Hit
GTACATGGGAAGCAGTGGTATCAAC738732.435657340305574No Hit
GTATCAACGCAGAGTACTTTTTTTT563601.858238432169022No Hit
GGTATCAACGCAGAGTACTTTTTTT384861.268917038688023No Hit
GAGTACATGGGAAGCAGTGGTATCA368021.2133940876629585No Hit
TATCAACGCAGAGTACTTTTTTTTT360561.1887978160093373No Hit
CATGTACTCTGCGTTGATACCACTG304561.0041609242395269No Hit
GCGTTGATACCACTGCTTCCCATGT255870.8436257410203827No Hit
ACGCAGAGTACATGGGAAGCAGTGG228460.7532525766737663No Hit
ACGCAGAGTACTTTTTTTTTTTTTT219060.7222599555552625No Hit
GTATCAACGCAGAGTACATGGGAAG214040.7057085770430402No Hit
TATCAACGCAGAGTACATGGGAAGC190710.6287875290967959No Hit
ACTCTGCGTTGATACCACTGCTTCC183690.6056419758785089No Hit
GGTATCAACGCAGAGTACATGGGAA160330.5286220153116736No Hit
GCTTCCCATGTACTCTGCGTTGATA136410.4497556858271403No Hit
GTACTTTTTTTTTTTTTTTTTTTTT128680.42426920058820045No Hit
CAGTGGTATCAACGCAGAGTACATG113850.37537339514273094No Hit
GTGGTATCAACGCAGAGTACATGGG113240.3733621718573812No Hit
GTACTGGTTCACTATCGGTCAGTCA112520.3709882689631979No Hit
GAGTACTTTTTTTTTTTTTTTTTTT112310.37029588061906116No Hit
CATGGGAAGCAGTGGTATCAACGCA94450.3114099004939037No Hit
ATACCACTGCTTCCCATGTACTCTG93600.3086073762438262No Hit
GCAGTGGTATCAACGCAGAGTACAT86330.2846375511872811No Hit
GGGAAGCAGTGGTATCAACGCAGAG86200.28420892983138696No Hit
ACATGGGAAGCAGTGGTATCAACGC81230.26782240568681626No Hit
GTACTCTGCGTTGATACCACTGCTT80620.26581118240146656No Hit
GGATACCACGTGTCCCGCCCTACTC76520.252293124254034No Hit
CTGCTTCCCATGTACTCTGCGTTGA75270.24817176506274358No Hit
GTTAATGATAGTGTGTCGAAACACA73100.2410170855066634No Hit
GCAGAGTACATGGGAAGCAGTGGTA73020.2407533185184208No Hit
TACCACTGCTTCCCATGTACTCTGC71650.2362363088447665No Hit
CCATGTACTCTGCGTTGATACCACT70830.23353269721528No Hit
GCAGAGTACTTTTTTTTTTTTTTTT68630.2262791050386089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA67080.22116861964140877No Hit
GATACCACTGCTTCCCATGTACTCT66380.21886065849428615No Hit
AAGCAGTGGTATCAACGCAGAGTAC65480.21589327987655704No Hit
CTTCCCATGTACTCTGCGTTGATAC64120.21140924107643308No Hit
ATACAGGGTGACAGCCCCGTACACA62420.2058041925762781No Hit
CCACTGCTTCCCATGTACTCTGCGT61570.20300166832620065No Hit
AGCGTACACGGTGGATGCCCTGGCA60110.1981879207907734No Hit
ATGGGAAGCAGTGGTATCAACGCAG59540.19630858099954498No Hit
ATCAACGCAGAGTACTTTTTTTTTT58020.19129700822293588No Hit
ACTTAGATGTTTCAGTTCCCCCGGT56770.18717564903164544No Hit
GTTGATACCACTGCTTCCCATGTAC56040.18476877526393184No Hit
GTACATGGTAAGCAGTGGTATCAAC55360.18252675586386988No Hit
GGTATCAACGCAGAGTACATGGGGG50600.16683262006343597No Hit
ACCCTGTATCGCGCGCCTTTCCAGA50010.1648873385251469No Hit
GTACATGGGGGTTAAGCGACTAAGC49800.1641949501810101No Hit
GTGGTATCAACGCAGAGTACTTTTT49660.16373335795158556No Hit
GTATCAACGCAGAGTACATGGGGGT48410.15961199876029514No Hit
ACCATGTACTCTGCGTTGATACCAC46380.15291891143363953No Hit
TCATTAACCTATGGATTCAGTTAAT45130.1487975522423491No Hit
CTATCGGTCAGTCAGGAGTATTTAG44860.14790733865703037No Hit
GTGGATGCCCTGGCAGTCAGAGGCG44400.1463906784746355No Hit
CCCATATTCAGACAGGATACCACGT41340.13630159117435658No Hit
ATCAACGCAGAGTACATGGGAAGCA40860.13471898924490106No Hit
TTCCCATGTACTCTGCGTTGATACC40800.13452116400371913No Hit
ATCATTAACTGAATCCATAGGTTAA40100.1322132028565965No Hit
GTTCACTATCGGTCAGTCAGGAGTA39970.13178458150070227No Hit
GTCAGGAGTATTTAGCCTTGGAGGA38810.12795996017118477No Hit
TGATACCACTGCTTCCCATGTACTC37090.12228896992396918No Hit
TATTCAGACAGGATACCACGTGTCC36680.1209371641092259No Hit
GTACTTAGATGTTTCAGTTCCCCCG36300.11968427091507362No Hit
CCTATGGATTCAGTTAATGATAGTG35330.11648609618263225No Hit
GTATTTAGCCTTGGAGGATGGTCCC34870.1149694360002374No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG34130.11252959135899347No Hit
CCCCATGTACTCTGCGTTGATACCA33580.11071619331482567No Hit
GCCTTGGAGGATGGTCCCCCCATAT33390.11008974671774954No Hit
GCCCAGAGCCTGAATCAGTGTGTGT32740.10794663993827852No Hit
ACGCAGAGTACATGGGGGTTAAGCG31390.10349557201168487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG456.854032E-414.75229916
GACCGTT1101.8007995E-1013.8459467
ATCGCCG3800.012.97758819
AATCGCC3750.012.89772518
CTGTCCG604.0303377E-412.6902279
GGACCGT1457.2759576E-1212.4732886
AAATCGC3900.012.40165817
CCGCTCC802.8144294E-511.8970879
CCGAACA802.9195646E-511.854526517
CCCGAAC658.1443135E-411.67214916
GAAATCG4150.011.65457116
CTAGAAC1104.962494E-711.2292063
GAACAAA9150.011.2226451
TAACGCC855.4209355E-511.15720119
TATACCG600.00579067111.1056025
AGGGCGC600.00579067111.1056028
CGTTATC600.00579709411.10394910
TACGGCC1303.3542165E-810.9426419
CGAACAC1408.591087E-910.83842518
CCCCGAA700.001513742310.83842515
Produced by FastQC (version 0.11.5)