##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3032980 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.85026046990089 31.0 30.0 33.0 27.0 34.0 2 30.960217014289576 31.0 31.0 34.0 27.0 34.0 3 31.131329583445982 31.0 31.0 34.0 27.0 34.0 4 34.60643525509565 37.0 35.0 37.0 31.0 37.0 5 34.92590323708036 37.0 35.0 37.0 32.0 37.0 6 34.30470527336151 37.0 35.0 37.0 30.0 37.0 7 34.16036142671564 36.0 35.0 37.0 30.0 37.0 8 34.181751280918434 37.0 35.0 37.0 30.0 37.0 9 35.45366504230163 39.0 35.0 39.0 28.0 39.0 10 35.34216743928414 39.0 35.0 39.0 28.0 39.0 11 35.47858706618573 39.0 35.0 39.0 28.0 39.0 12 35.4586080356613 39.0 35.0 39.0 28.0 39.0 13 35.23975957639022 39.0 35.0 39.0 27.0 39.0 14 36.201596119987606 39.0 36.0 41.0 27.0 41.0 15 36.18853800552592 39.0 36.0 41.0 27.0 41.0 16 36.211992166120446 39.0 36.0 41.0 27.0 41.0 17 36.38963659503195 39.0 36.0 41.0 27.0 41.0 18 36.413270117178484 39.0 36.0 41.0 27.0 41.0 19 36.38303022110268 39.0 36.0 41.0 27.0 41.0 20 36.53617432360253 39.0 36.0 41.0 27.0 41.0 21 36.377253064642694 39.0 36.0 41.0 27.0 41.0 22 36.446232088572955 39.0 36.0 41.0 27.0 41.0 23 36.46232286398196 39.0 36.0 41.0 27.0 41.0 24 36.36182764146153 39.0 35.0 41.0 27.0 41.0 25 35.23864878766098 38.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5167053864192184 1101 2 0.5207582529233932 1101 3 0.37923655671042766 1101 4 0.2971348556640123 1101 5 0.4125343932065135 1101 6 0.4241339540216913 1101 7 0.4466885635338045 1101 8 0.39057596747379364 1101 9 0.6068823293021239 1101 10 0.554741668710875 1101 11 0.6093242677765858 1101 12 0.7586837008410683 1101 13 0.7616327527520781 1101 14 0.9765459520972115 1101 15 1.0040978760433177 1101 16 0.9190932692376705 1101 17 0.880363557785131 1101 18 0.9238295316843335 1101 19 0.9948411370100843 1101 20 0.9428620767984413 1101 21 1.1591357261815745 1101 22 1.0251984071512723 1101 23 1.08286768569333 1101 24 1.2730676852142224 1101 25 1.2438802463152854 1102 1 0.3826466280644212 1102 2 0.4008465161708088 1102 3 0.40645564598928985 1102 4 0.3972840901585428 1102 5 0.37202694518233415 1102 6 0.5099003398778521 1102 7 0.470346144453309 1102 8 0.48914034660552375 1102 9 0.589589224077848 1102 10 0.8031262725417463 1102 11 0.6628803466592714 1102 12 0.7750265525037108 1102 13 0.77867227096975 1102 14 1.0050820383274512 1102 15 0.9468319613337997 1102 16 0.9403808455294325 1102 17 0.896056299386693 1102 18 0.8883406073093312 1102 19 0.9533525140471539 1102 20 0.898467573813754 1102 21 1.1314028029005883 1102 22 1.0378491829575225 1102 23 1.0010386852853586 1102 24 1.145227865939681 1102 25 0.9392430292316192 1103 1 0.37599541004113846 1103 2 0.38813956588660403 1103 3 0.27981740933869403 1103 4 0.21842554850642415 1103 5 0.3230363640533156 1103 6 0.2862191835638157 1103 7 0.3758908219386541 1103 8 0.3834397798419147 1103 9 0.22556476425610583 1103 10 0.5877140163383956 1103 11 0.5951871738981112 1103 12 0.5555218231102259 1103 13 0.7162970355413663 1103 14 0.9099075772691805 1103 15 0.8311589062293478 1103 16 0.8185902317734275 1103 17 0.8330853612612472 1103 18 0.7627636152304831 1103 19 0.9056251436992611 1103 20 0.7419923549157872 1103 21 1.0292070332140852 1103 22 0.9738200373461794 1103 23 0.9667111546661076 1103 24 1.0674934365365232 1103 25 0.9592450597404465 1104 1 0.20006104913567668 1104 2 0.19480295925519897 1104 3 0.06744258424710381 1104 4 0.13801299121525545 1104 5 0.21572324806967913 1104 6 0.15948810596568563 1104 7 0.27693476055484467 1104 8 0.23329887247557224 1104 9 -0.12044617424037796 1104 10 0.31200560768200347 1104 11 0.39782556035152794 1104 12 0.5196105646556148 1104 13 0.047625811956841346 1104 14 0.36131161248824384 1104 15 0.4671140566353671 1104 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2207 1 0.11253411590499596 2207 2 0.13233029473990854 2207 3 0.1621504634508888 2207 4 0.14534749076579345 2207 5 0.08837540239848352 2207 6 0.23481701252973863 2207 7 0.20596466121494217 2207 8 0.1640214929389643 2207 9 0.13846391948654713 2207 10 0.4377701117932631 2207 11 0.322541729473528 2207 12 0.12747904844168545 2207 13 0.1865061499222591 2207 14 0.37810759148146644 2207 15 0.37633351275364646 2207 16 0.40196573539432734 2207 17 0.46725040033425813 2207 18 0.36690197397006585 2207 19 0.4833437275528141 2207 20 0.33033031441939187 2207 21 0.7690339448201442 2207 22 0.5897481318018762 2207 23 0.5277356880832826 2207 24 0.7299613781455747 2207 25 0.7119437786968348 2208 1 0.2566764955355829 2208 2 0.24765225005547364 2208 3 0.21747548304784914 2208 4 0.202338319747291 2208 5 0.2199266204720587 2208 6 0.35832780380602713 2208 7 0.337186539619438 2208 8 0.33213120594002277 2208 9 0.44626870499934057 2208 10 0.4542092973719818 2208 11 0.06956719395593325 2208 12 -0.1737917976572234 2208 13 0.09428050979202851 2208 14 -0.2410135515497842 2208 15 0.3119420967374893 2208 16 0.44756814955291446 2208 17 0.32357645833387494 2208 18 0.37835708740549023 2208 19 0.5731111559789852 2208 20 0.48734338170100244 2208 21 0.8412043953096813 2208 22 0.8679177492238495 2208 23 0.7195051933147667 2208 24 0.8292291918872152 2208 25 0.7767505069119025 2209 1 -0.00414447756388725 2209 2 0.04168272582845134 2209 3 0.1504444963726712 2209 4 -0.40020208907117194 2209 5 0.0351509151342313 2209 6 0.16450144716191062 2209 7 0.2378456088319112 2209 8 0.2954792646459623 2209 9 0.37794824092178203 2209 10 0.26866466004539546 2209 11 0.39410180825162655 2209 12 0.3760916242378656 2209 13 0.3722553600804943 2209 14 0.43776286722454927 2209 15 -0.1542363118155805 2209 16 -0.052606287823422804 2209 17 0.2386217223349334 2209 18 -0.13496789743130222 2209 19 0.3256546807099312 2209 20 0.40733252435768463 2209 21 0.6331322479678505 2209 22 0.6628202507106238 2209 23 0.5302947386762753 2209 24 0.7056961822864452 2209 25 0.6376221135892024 2210 1 -0.1436531086469195 2210 2 -0.15909793436834718 2210 3 -0.011840901848991336 2210 4 0.06059947960304157 2210 5 -0.1018536624855031 2210 6 -0.47955550657457024 2210 7 -0.24096009977860433 2210 8 -0.31163986049144654 2210 9 -0.0598765367926859 2210 10 0.11739956059092549 2210 11 0.01782115087817715 2210 12 0.07147073516927094 2210 13 0.06282315747968426 2210 14 0.08332950705416664 2210 15 -0.03638905768487177 2210 16 0.03325585463498015 2210 17 -0.9151587239445789 2210 18 -1.3765591951344263 2210 19 -0.37673106822314395 2210 20 -0.19040226364818835 2210 21 -0.3471350711574672 2210 22 -0.2309035160247248 2210 23 -0.07621160339991917 2210 24 0.26697510560359916 2210 25 0.2212133317135212 2211 1 -0.2470692130234866 2211 2 -0.2454950267643845 2211 3 -0.15798424157771151 2211 4 -0.11744694310377213 2211 5 -0.15422277714025512 2211 6 -0.09989430910705721 2211 7 -0.14226529457812376 2211 8 -0.16595376955163488 2211 9 -0.05091642206114244 2211 10 -0.08700008460521502 2211 11 -0.42896599779604117 2211 12 -0.2773211326835465 2211 13 -0.1589744462874947 2211 14 -0.19755479248826902 2211 15 -0.15067474773545086 2211 16 -0.15637621860285833 2211 17 -0.40272997291474155 2211 18 -0.3085802429986231 2211 19 -0.20410690045900992 2211 20 -0.2890328154536448 2211 21 0.11297911698617469 2211 22 -0.049691871411631894 2211 23 -0.7134549599262314 2211 24 -0.23275795387879583 2211 25 -0.7992126741271264 2212 1 -0.14926731040472418 2212 2 -0.2740142013850786 2212 3 -0.09744016310202142 2212 4 -0.06169360140700064 2212 5 -0.10240438677629982 2212 6 -0.06321094133043204 2212 7 -0.2081105069196738 2212 8 -0.32139681009251575 2212 9 -0.07756865503385768 2212 10 -0.46696865650785213 2212 11 -0.12965620122123056 2212 12 -0.33717659467599503 2212 13 -0.26226077041232543 2212 14 -0.2584932800428632 2212 15 -0.17544458507057925 2212 16 -0.3168207019315403 2212 17 -0.584719062564254 2212 18 -0.3501842940044284 2212 19 -0.4528758364775811 2212 20 -0.6127754526869396 2212 21 -0.0857163170328974 2212 22 -0.46239132861932575 2212 23 -0.37206743925548835 2212 24 -0.53177080121241 2212 25 -0.2272300261325313 2213 1 -0.10215415333570022 2213 2 -0.13899316010975937 2213 3 -0.0427512484572361 2213 4 0.03957690943051517 2213 5 0.015270215378073715 2213 6 -0.14165223862578813 2213 7 -0.1618849260643671 2213 8 -0.10012502758652886 2213 9 -0.07673628236312169 2213 10 -0.07605889175269454 2213 11 0.19429624459809247 2213 12 0.20545747304587536 2213 13 0.25633833126448025 2213 14 -0.547107510427729 2213 15 -0.03614833607789336 2213 16 -0.025624777214311223 2213 17 0.08293046906089785 2213 18 0.09001826066694463 2213 19 0.017880108226954405 2213 20 -0.057486728654204455 2213 21 0.20319950266109998 2213 22 -0.08669096066481785 2213 23 -0.28778337823751343 2213 24 -0.46990870882016367 2213 25 -0.17465957984600777 2214 1 -0.2061668699628676 2214 2 -0.04913995412672989 2214 3 -0.11254458733985118 2214 4 -0.020441132806297446 2214 5 -0.10326282123867969 2214 6 -0.027214450013701708 2214 7 -0.17885429036041955 2214 8 -0.0880109290188642 2214 9 -0.21514816206833132 2214 10 -0.21727372390114397 2214 11 -0.06280969992609897 2214 12 -0.1188908405110709 2214 13 -0.25165840003997175 2214 14 -0.10274947855334204 2214 15 -0.04966090264801437 2214 16 -0.17126947751074084 2214 17 -0.2701036915680959 2214 18 -0.0948194653741794 2214 19 -0.2586640637554112 2214 20 -0.32780494869446386 2214 21 -0.40432132837182877 2214 22 -0.7466986687840418 2214 23 -0.9285590103882271 2214 24 -0.5388512525419742 2214 25 -0.7608498366134455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 33.0 10 94.0 11 254.0 12 834.0 13 2374.0 14 5282.0 15 9925.0 16 15180.0 17 18936.0 18 21455.0 19 22653.0 20 22864.0 21 22942.0 22 22857.0 23 23366.0 24 24617.0 25 26389.0 26 28680.0 27 31369.0 28 35892.0 29 44224.0 30 55278.0 31 71062.0 32 93285.0 33 125208.0 34 169225.0 35 233536.0 36 332702.0 37 526874.0 38 1045588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.437346051391444 20.28075738095301 11.862722062205137 22.419174505450407 2 13.656305020145204 23.825049950873396 36.778119209490335 25.74052581949106 3 20.42921855808978 30.926616833291515 27.717712859388 20.926451749230708 4 11.930958208110669 20.378335671262725 34.22382392918547 33.46688219144113 5 11.308163846801236 40.98854465395199 32.87043876982986 14.832852729416917 6 29.857213049342292 35.412977454171326 18.40157758145946 16.328231915026926 7 23.185035265903295 36.52981664764338 21.648222161174758 18.636925925278565 8 26.913090866519035 34.25555806556398 21.520031790997276 17.311319276919704 9 27.573691599477478 16.498372226900425 20.84202391181209 35.08591226181001 10 17.416241245862448 31.452116249254562 29.7106211904899 21.42102131439309 11 29.05156856729053 28.377327117788532 19.348621462104642 23.222482852816295 12 23.223838590459195 31.6125556433694 28.664681128584856 16.498924637586544 13 29.943177959088395 23.208246152791084 24.671593397644166 22.17698249047636 14 22.32553937621087 26.78169886196532 27.56928242752596 23.32347933429785 15 29.6598154531145 25.3141009620362 24.819828404235885 20.20625518061341 16 18.576296959621864 29.643043899428218 30.29079010794012 21.4898690330098 17 21.78979089872007 29.421295227795763 28.273018615355195 20.515895258128968 18 22.375446057205444 24.75925357480457 32.031350201106974 20.83395016688301 19 20.849925980144874 29.1568990131785 33.234684154475644 16.75849085220098 20 23.09055276474692 24.242920805071723 32.17477774275529 20.49174868742606 21 20.769752978348308 27.53545778350153 32.53065556468851 19.16413367346165 22 21.422092444713495 26.94017540406273 28.266438237149274 23.371293914074496 23 19.10867653619073 27.372647234248376 31.765993300962453 21.75268292859844 24 20.62361790173951 30.02582609375139 29.442867886655332 19.907688117853766 25 21.123126273728314 25.300872721301896 28.973397645245008 24.60260335972478 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8456.0 1 8456.0 2 8561.0 3 8666.0 4 8666.0 5 8666.0 6 15262.0 7 21858.0 8 21858.0 9 21858.0 10 23167.0 11 24476.0 12 24476.0 13 24476.0 14 24068.0 15 23660.0 16 23660.0 17 23660.0 18 37096.0 19 50532.0 20 50532.0 21 50532.0 22 75173.5 23 99815.0 24 99815.0 25 99815.0 26 134192.0 27 168569.0 28 168569.0 29 168569.0 30 191363.5 31 214158.0 32 214158.0 33 214158.0 34 227023.5 35 239889.0 36 239889.0 37 239889.0 38 227696.0 39 215503.0 40 215503.0 41 215503.0 42 243060.0 43 270617.0 44 270617.0 45 270617.0 46 452429.0 47 634241.0 48 634241.0 49 634241.0 50 561933.5 51 489626.0 52 489626.0 53 489626.0 54 370309.5 55 250993.0 56 250993.0 57 250993.0 58 193088.5 59 135184.0 60 135184.0 61 135184.0 62 119073.5 63 102963.0 64 102963.0 65 102963.0 66 73000.0 67 43037.0 68 43037.0 69 43037.0 70 31741.5 71 20446.0 72 20446.0 73 20446.0 74 13950.0 75 7454.0 76 7454.0 77 7454.0 78 4783.0 79 2112.0 80 2112.0 81 2112.0 82 1306.5 83 501.0 84 501.0 85 501.0 86 311.5 87 122.0 88 122.0 89 122.0 90 95.5 91 69.0 92 69.0 93 69.0 94 48.5 95 28.0 96 28.0 97 28.0 98 16.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09060396046132846 2 0.0 3 0.13913708629796437 4 0.012594873688583506 5 0.022552077494741148 6 0.016155728029858422 7 0.024101708550666342 8 0.022255339632968236 9 6.594174706064662E-5 10 0.3714498611926224 11 0.0616555335017046 12 0.0016155728029858421 13 6.594174706064662E-5 14 0.001055067952970346 15 0.0012528931941522858 16 6.594174706064662E-5 17 0.0 18 5.605048500154963E-4 19 3.2970873530323313E-4 20 2.637669882425865E-4 21 2.3079611471226317E-4 22 0.0031981747324413613 23 2.3079611471226317E-4 24 3.297087353032331E-5 25 0.0017474562971071354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3032980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.349996949401365 #Duplication Level Percentage of deduplicated Percentage of total 1 75.20503673176131 26.584978190473795 2 13.92452020769511 9.844634937277995 3 4.982270409195884 5.283697312985016 4 2.2952869839737158 3.24553551525886 5 1.152264965106751 2.0366281500712855 6 0.6609267196043367 1.401825451307469 7 0.4108363100318728 1.0166143614431011 8 0.26903847303755996 0.7608407360919476 9 0.17442133716473557 0.5549214363015519 >10 0.764047745710658 4.785633192977799 >50 0.06825508906084958 1.6767151602158543 >100 0.061499341661447285 4.797576751283223 >500 0.014946462237590006 3.6690073803858567 >1k 0.012203124231956393 9.006788118866057 >5k 0.002554142281107152 6.236456473915494 >10k+ 0.0018919572452645568 19.09814683114481 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 82161 2.708919940124894 No Hit GTACATGGGAAGCAGTGGTATCAAC 73873 2.435657340305574 No Hit GTATCAACGCAGAGTACTTTTTTTT 56360 1.858238432169022 No Hit GGTATCAACGCAGAGTACTTTTTTT 38486 1.268917038688023 No Hit GAGTACATGGGAAGCAGTGGTATCA 36802 1.2133940876629585 No Hit TATCAACGCAGAGTACTTTTTTTTT 36056 1.1887978160093373 No Hit CATGTACTCTGCGTTGATACCACTG 30456 1.0041609242395269 No Hit GCGTTGATACCACTGCTTCCCATGT 25587 0.8436257410203827 No Hit ACGCAGAGTACATGGGAAGCAGTGG 22846 0.7532525766737663 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 21906 0.7222599555552625 No Hit GTATCAACGCAGAGTACATGGGAAG 21404 0.7057085770430402 No Hit TATCAACGCAGAGTACATGGGAAGC 19071 0.6287875290967959 No Hit ACTCTGCGTTGATACCACTGCTTCC 18369 0.6056419758785089 No Hit GGTATCAACGCAGAGTACATGGGAA 16033 0.5286220153116736 No Hit GCTTCCCATGTACTCTGCGTTGATA 13641 0.4497556858271403 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 12868 0.42426920058820045 No Hit CAGTGGTATCAACGCAGAGTACATG 11385 0.37537339514273094 No Hit GTGGTATCAACGCAGAGTACATGGG 11324 0.3733621718573812 No Hit GTACTGGTTCACTATCGGTCAGTCA 11252 0.3709882689631979 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 11231 0.37029588061906116 No Hit CATGGGAAGCAGTGGTATCAACGCA 9445 0.3114099004939037 No Hit ATACCACTGCTTCCCATGTACTCTG 9360 0.3086073762438262 No Hit GCAGTGGTATCAACGCAGAGTACAT 8633 0.2846375511872811 No Hit GGGAAGCAGTGGTATCAACGCAGAG 8620 0.28420892983138696 No Hit ACATGGGAAGCAGTGGTATCAACGC 8123 0.26782240568681626 No Hit GTACTCTGCGTTGATACCACTGCTT 8062 0.26581118240146656 No Hit GGATACCACGTGTCCCGCCCTACTC 7652 0.252293124254034 No Hit CTGCTTCCCATGTACTCTGCGTTGA 7527 0.24817176506274358 No Hit GTTAATGATAGTGTGTCGAAACACA 7310 0.2410170855066634 No Hit GCAGAGTACATGGGAAGCAGTGGTA 7302 0.2407533185184208 No Hit TACCACTGCTTCCCATGTACTCTGC 7165 0.2362363088447665 No Hit CCATGTACTCTGCGTTGATACCACT 7083 0.23353269721528 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 6863 0.2262791050386089 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 6708 0.22116861964140877 No Hit GATACCACTGCTTCCCATGTACTCT 6638 0.21886065849428615 No Hit AAGCAGTGGTATCAACGCAGAGTAC 6548 0.21589327987655704 No Hit CTTCCCATGTACTCTGCGTTGATAC 6412 0.21140924107643308 No Hit ATACAGGGTGACAGCCCCGTACACA 6242 0.2058041925762781 No Hit CCACTGCTTCCCATGTACTCTGCGT 6157 0.20300166832620065 No Hit AGCGTACACGGTGGATGCCCTGGCA 6011 0.1981879207907734 No Hit ATGGGAAGCAGTGGTATCAACGCAG 5954 0.19630858099954498 No Hit ATCAACGCAGAGTACTTTTTTTTTT 5802 0.19129700822293588 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 5677 0.18717564903164544 No Hit GTTGATACCACTGCTTCCCATGTAC 5604 0.18476877526393184 No Hit GTACATGGTAAGCAGTGGTATCAAC 5536 0.18252675586386988 No Hit GGTATCAACGCAGAGTACATGGGGG 5060 0.16683262006343597 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 5001 0.1648873385251469 No Hit GTACATGGGGGTTAAGCGACTAAGC 4980 0.1641949501810101 No Hit GTGGTATCAACGCAGAGTACTTTTT 4966 0.16373335795158556 No Hit GTATCAACGCAGAGTACATGGGGGT 4841 0.15961199876029514 No Hit ACCATGTACTCTGCGTTGATACCAC 4638 0.15291891143363953 No Hit TCATTAACCTATGGATTCAGTTAAT 4513 0.1487975522423491 No Hit CTATCGGTCAGTCAGGAGTATTTAG 4486 0.14790733865703037 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 4440 0.1463906784746355 No Hit CCCATATTCAGACAGGATACCACGT 4134 0.13630159117435658 No Hit ATCAACGCAGAGTACATGGGAAGCA 4086 0.13471898924490106 No Hit TTCCCATGTACTCTGCGTTGATACC 4080 0.13452116400371913 No Hit ATCATTAACTGAATCCATAGGTTAA 4010 0.1322132028565965 No Hit GTTCACTATCGGTCAGTCAGGAGTA 3997 0.13178458150070227 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 3881 0.12795996017118477 No Hit TGATACCACTGCTTCCCATGTACTC 3709 0.12228896992396918 No Hit TATTCAGACAGGATACCACGTGTCC 3668 0.1209371641092259 No Hit GTACTTAGATGTTTCAGTTCCCCCG 3630 0.11968427091507362 No Hit CCTATGGATTCAGTTAATGATAGTG 3533 0.11648609618263225 No Hit GTATTTAGCCTTGGAGGATGGTCCC 3487 0.1149694360002374 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 3413 0.11252959135899347 No Hit CCCCATGTACTCTGCGTTGATACCA 3358 0.11071619331482567 No Hit GCCTTGGAGGATGGTCCCCCCATAT 3339 0.11008974671774954 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 3274 0.10794663993827852 No Hit ACGCAGAGTACATGGGGGTTAAGCG 3139 0.10349557201168487 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.297087353032331E-5 0.0 8 0.0 0.0 0.0 3.297087353032331E-5 0.0 9 0.0 0.0 0.0 3.297087353032331E-5 0.0 10 0.0 0.0 0.0 6.594174706064662E-5 0.0 11 0.0 0.0 0.0 9.891262059096993E-5 0.0 12 0.0 0.0 0.0 2.307961147122632E-4 0.0 13 0.0 0.0 0.0 2.637669882425865E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACG 45 6.854032E-4 14.752299 16 GACCGTT 110 1.8007995E-10 13.845946 7 ATCGCCG 380 0.0 12.977588 19 AATCGCC 375 0.0 12.897725 18 CTGTCCG 60 4.0303377E-4 12.690227 9 GGACCGT 145 7.2759576E-12 12.473288 6 AAATCGC 390 0.0 12.401658 17 CCGCTCC 80 2.8144294E-5 11.897087 9 CCGAACA 80 2.9195646E-5 11.8545265 17 CCCGAAC 65 8.1443135E-4 11.672149 16 GAAATCG 415 0.0 11.654571 16 CTAGAAC 110 4.962494E-7 11.229206 3 GAACAAA 915 0.0 11.222645 1 TAACGCC 85 5.4209355E-5 11.157201 19 TATACCG 60 0.005790671 11.105602 5 AGGGCGC 60 0.005790671 11.105602 8 CGTTATC 60 0.005797094 11.103949 10 TACGGCC 130 3.3542165E-8 10.94264 19 CGAACAC 140 8.591087E-9 10.838425 18 CCCCGAA 70 0.0015137423 10.838425 15 >>END_MODULE