FastQCFastQC Report
Wed 25 May 2016
SRR1294617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712767
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC616721.6610791897256143No Hit
CCCATGTACTCTGCGTTGATACCAC603091.624368025249093No Hit
GAGTACATGGGAAGCAGTGGTATCA278230.7493871821205047No Hit
CATGTACTCTGCGTTGATACCACTG222300.5987448175444352No Hit
GCGTTGATACCACTGCTTCCCATGT192830.519370054732764No Hit
GTATCAACGCAGAGTACATGGGAAG167190.4503110483367257No Hit
ACGCAGAGTACATGGGAAGCAGTGG144200.3883895757530704No Hit
TATCAACGCAGAGTACATGGGAAGC143750.3871775417094582No Hit
GTATCAACGCAGAGTACTTTTTTTT136250.36697697431592124No Hit
ACTCTGCGTTGATACCACTGCTTCC129470.3487156613921638No Hit
GGTATCAACGCAGAGTACATGGGAA118610.31946523980632235No Hit
GTACTGGTTCACTATCGGTCAGTCA103350.27836381868293913No Hit
GCTTCCCATGTACTCTGCGTTGATA100340.2702566576356663No Hit
GGTATCAACGCAGAGTACTTTTTTT83400.22463030941613088No Hit
TATCAACGCAGAGTACTTTTTTTTT81760.2202131186794108No Hit
GTGGTATCAACGCAGAGTACATGGG77790.20952028500576522No Hit
CAGTGGTATCAACGCAGAGTACATG74960.2018979375759373No Hit
ATACCACTGCTTCCCATGTACTCTG71000.19123203799214977No Hit
GTACTCTGCGTTGATACCACTGCTT65270.17579880450348756No Hit
GCAGTGGTATCAACGCAGAGTACAT61800.16645267532274446No Hit
GGGAAGCAGTGGTATCAACGCAGAG59580.16047330737425752No Hit
GGATACCACGTGTCCCGCCCTACTC58530.15764522793916236No Hit
CATGGGAAGCAGTGGTATCAACGCA56620.15250081677627494No Hit
GTACATGGTAAGCAGTGGTATCAAC55940.1506692986659276No Hit
CTGCTTCCCATGTACTCTGCGTTGA55370.1491340555440188No Hit
TACCACTGCTTCCCATGTACTCTGC54410.14654838291764608No Hit
ACATGGGAAGCAGTGGTATCAACGC52770.14213119218092599No Hit
GCAGAGTACATGGGAAGCAGTGGTA51330.13825268324136689No Hit
GTTAATGATAGTGTGTCGAAACACA49600.13359308569592435No Hit
CCATGTACTCTGCGTTGATACCACT49570.13351228342635021No Hit
GTTGATACCACTGCTTCCCATGTAC48650.13103434715940968No Hit
GATACCACTGCTTCCCATGTACTCT47920.12906815859977208No Hit
CTTCCCATGTACTCTGCGTTGATAC46470.12516271557035494No Hit
ATACAGGGTGACAGCCCCGTACACA46270.12462403377319395No Hit
AAGCAGTGGTATCAACGCAGAGTAC45070.12139194299022804No Hit
ATTCCATTCCATTCCATTCCATTCC44360.11947962261030655No Hit
CCACTGCTTCCCATGTACTCTGCGT43220.11640913636648893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42630.11482002506486401No Hit
ACCATGTACTCTGCGTTGATACCAC41140.11080684567601469No Hit
ATGGGAAGCAGTGGTATCAACGCAG40430.10889452529609317No Hit
GTTCACTATCGGTCAGTCAGGAGTA38700.10423492775065066No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37240.10030255063137547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG1701.2732926E-1111.1722695
CCGTACA13200.011.01776717
CGCAGAA2600.010.9580092
CCCGTAC13200.010.87374416
CGCGAAA700.001496430610.85349919
CCCCGTA13050.010.70722815
TACTGGT24300.010.630322
GACCGTT909.558685E-510.5515877
GTACTGG26100.010.3377371
CGCGCCT10400.010.327712
GCGCCTT10450.010.27856313
ATCGCCG2500.010.25655719
CTGGTTC26050.010.2436094
AATCGCC2600.010.23713318
GCAACCG1409.757059E-810.1746089
ACTGGTT25200.010.1376073
ATACCGT750.00265732710.1295236
GGGGCGC750.002657624210.1293888
GTCTGCG951.6516971E-49.9961069
TGGTTCA26050.09.9885655
Produced by FastQC (version 0.11.5)