FastQCFastQC Report
Wed 25 May 2016
SRR1294617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712767
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC665931.7936218459170747No Hit
GTACATGGGAAGCAGTGGTATCAAC599131.6137021256653057No Hit
GTATCAACGCAGAGTACTTTTTTTT349820.9422083314142795No Hit
GAGTACATGGGAAGCAGTGGTATCA294130.792212384994803No Hit
CATGTACTCTGCGTTGATACCACTG243830.6567339130088152No Hit
TATCAACGCAGAGTACTTTTTTTTT233500.6289109981854504No Hit
GGTATCAACGCAGAGTACTTTTTTT230900.6219081348223575No Hit
GCGTTGATACCACTGCTTCCCATGT201430.5425333720106864No Hit
ACGCAGAGTACATGGGAAGCAGTGG177510.4781070290702325No Hit
GTATCAACGCAGAGTACATGGGAAG168370.4534892709399755No Hit
TATCAACGCAGAGTACATGGGAAGC157360.42383483800626326No Hit
ACTCTGCGTTGATACCACTGCTTCC141740.3817637896479903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT133250.35889674735850646No Hit
GGTATCAACGCAGAGTACATGGGAA127870.34440620701487595No Hit
GCTTCCCATGTACTCTGCGTTGATA105720.2847471979792968No Hit
GTACTGGTTCACTATCGGTCAGTCA93890.2528841696772246No Hit
GTACTTTTTTTTTTTTTTTTTTTTT90810.24458847000094538No Hit
CAGTGGTATCAACGCAGAGTACATG90590.2439959200240683No Hit
GTGGTATCAACGCAGAGTACATGGG79740.21477243252808484No Hit
ATACCACTGCTTCCCATGTACTCTG76160.20513002835890323No Hit
GAGTACTTTTTTTTTTTTTTTTTTT72930.1964303173347533No Hit
CATGGGAAGCAGTGGTATCAACGCA71720.19317129246192935No Hit
GCAGTGGTATCAACGCAGAGTACAT70970.19115123572257564No Hit
GGGAAGCAGTGGTATCAACGCAGAG67440.18164350200268425No Hit
ACATGGGAAGCAGTGGTATCAACGC64120.1727013841698119No Hit
GTACTCTGCGTTGATACCACTGCTT63220.17027731608258745No Hit
GGATACCACGTGTCCCGCCCTACTC61220.1648904981109776No Hit
GCAGAGTACATGGGAAGCAGTGGTA58800.15837244836532968No Hit
CTGCTTCCCATGTACTCTGCGTTGA58300.1570257438724272No Hit
CCATGTACTCTGCGTTGATACCACT58160.1566486666144145No Hit
TACCACTGCTTCCCATGTACTCTGC56670.1526354872255652No Hit
GTACATGGTAAGCAGTGGTATCAAC55730.15010368277890856No Hit
AAGCAGTGGTATCAACGCAGAGTAC52870.14240053307950645No Hit
GATACCACTGCTTCCCATGTACTCT51980.14000339908214007No Hit
ATACAGGGTGACAGCCCCGTACACA50660.13644809922087758No Hit
CTTCCCATGTACTCTGCGTTGATAC49730.133943228864079No Hit
ATGGGAAGCAGTGGTATCAACGCAG49440.13316214025819556No Hit
GTTAATGATAGTGTGTCGAAACACA49190.13248878801174435No Hit
CCACTGCTTCCCATGTACTCTGCGT48020.12933749949835258No Hit
GTTGATACCACTGCTTCCCATGTAC47010.12661715642268961No Hit
ACCATGTACTCTGCGTTGATACCAC45240.12184982251781488No Hit
ATTCCATTCCATTCCATTCCATTCC42570.11465842052571572No Hit
GCAGAGTACTTTTTTTTTTTTTTTT42120.11344638648210352No Hit
ACCCTGTATCGCGCGCCTTTCCAGA40100.10800570033077757No Hit
AGCGTACACGGTGGATGCCCTGGCA38050.10248421190987747No Hit
GAATGGAATGGAATGGAATGGAATG37680.10148765058512965No Hit
ACTTAGATGTTTCAGTTCCCCCGGT37600.10127217786626524No Hit
CTATCGGTCAGTCAGGAGTATTTAG37560.10116444150683306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGC250.00595823419.0414054
ACCCGTA551.9924606E-413.7925315
CCCGAAC1257.2759576E-1213.65460516
CCGAACA1251.05501385E-1012.89601617
GACCGTT1156.873415E-811.590427
AATCGCC2100.011.28852918
CCCCGTA13300.010.76569215
CCGTACA13950.010.739881517
GGACCGT1605.2568794E-1010.710796
CGAACAC1701.4370016E-1010.59793718
CCCGTAC14050.010.59595116
AAATCGC2350.010.49112717
TGTCCCG12450.010.21238211
TACCGTT750.00260164110.15541657
ATCGCCG2350.010.08775819
CCCCCGG8700.09.7003518
TGTCGAA12750.09.66829314
GTACTGG21850.09.6604061
CCATGCG1002.697891E-49.5205739
GCGTACT1201.7394179E-59.482491512
Produced by FastQC (version 0.11.5)