##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294616_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4056843 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.105523432876254 31.0 30.0 33.0 26.0 34.0 2 30.20973500823177 31.0 30.0 34.0 26.0 34.0 3 30.207914627211355 31.0 30.0 34.0 26.0 34.0 4 33.816923898706456 35.0 35.0 37.0 28.0 37.0 5 34.23025194714215 35.0 35.0 37.0 30.0 37.0 6 34.3996445018947 37.0 35.0 37.0 30.0 37.0 7 34.320945622002135 37.0 35.0 37.0 30.0 37.0 8 34.1391850756857 37.0 35.0 37.0 29.0 37.0 9 35.34803220139404 39.0 35.0 39.0 28.0 39.0 10 35.448548292354424 39.0 35.0 39.0 28.0 39.0 11 35.682201406364506 39.0 35.0 39.0 30.0 39.0 12 35.47695806813328 39.0 35.0 39.0 28.0 39.0 13 35.33558533076089 39.0 35.0 39.0 27.0 39.0 14 36.26436467962896 39.0 36.0 41.0 27.0 41.0 15 36.25532291981721 39.0 36.0 41.0 27.0 41.0 16 36.363754525378475 39.0 36.0 41.0 27.0 41.0 17 36.352772093965676 39.0 36.0 41.0 27.0 41.0 18 36.224756787482285 39.0 36.0 41.0 27.0 41.0 19 36.486367848102574 39.0 36.0 41.0 27.0 41.0 20 36.600662879978344 40.0 36.0 41.0 28.0 41.0 21 36.63942257563332 40.0 36.0 41.0 28.0 41.0 22 36.64179387765314 39.0 36.0 41.0 28.0 41.0 23 36.6119642786275 39.0 36.0 41.0 28.0 41.0 24 36.62771519627454 39.0 36.0 41.0 28.0 41.0 25 35.46378624955415 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9071267844697495 1101 2 0.883546948343767 1101 3 1.2275347456944203 1101 4 1.0255054212249064 1101 5 0.6840195154420528 1101 6 0.7158279089359141 1101 7 0.7419482958631036 1101 8 0.9328127129307333 1101 9 1.1678339634544628 1101 10 1.0605175295743337 1101 11 0.8680639910166974 1101 12 1.037878175229146 1101 13 1.1747364174982309 1101 14 1.2999680360974466 1101 15 1.4421932175284908 1101 16 1.3047377796411936 1101 17 1.2785294873950974 1101 18 1.2627479116680433 1101 19 1.1295555124697643 1101 20 1.1564716222521696 1101 21 1.0515546586378903 1101 22 1.0023829959313844 1101 23 1.0021040340540068 1101 24 1.0725420355373458 1101 25 0.9966158131718501 1102 1 0.8761561240190829 1102 2 0.8400615136641605 1102 3 1.1407275796133547 1102 4 1.0545312517836294 1102 5 0.8573036258304185 1102 6 0.7545866581949809 1102 7 0.7300697931420146 1102 8 0.7576057119314257 1102 9 1.160928882168136 1102 10 1.0271208635554885 1102 11 0.7601683867878677 1102 12 1.0055622291319537 1102 13 0.9534582531080389 1102 14 1.1919387899356764 1102 15 1.2271376221608037 1102 16 1.1882032113916807 1102 17 1.256198858806222 1102 18 1.2674750501120897 1102 19 0.9942405313773435 1102 20 0.21961863284392535 1102 21 0.6465775029951288 1102 22 0.7528342558771115 1102 23 0.7897151615278943 1102 24 0.8354207332526897 1102 25 0.9158039277508152 1103 1 0.6611383465118124 1103 2 0.6512979887471175 1103 3 0.9494691806287747 1103 4 0.7792541597882021 1103 5 0.5701188373916324 1103 6 0.4639265065662812 1103 7 0.39653947437685133 1103 8 0.5486242984294023 1103 9 0.6359163605644014 1103 10 0.6792246197830067 1103 11 0.6003217146180333 1103 12 0.7704432834066068 1103 13 0.631131853256143 1103 14 0.5864032900795948 1103 15 0.8529737165870088 1103 16 0.7926168389541672 1103 17 0.7159855223789435 1103 18 0.8799822302215716 1103 19 0.7406656927633861 1103 20 -0.09872082498014123 1103 21 0.23309760032761773 1103 22 -0.28397612076004464 1103 23 0.31793920503587003 1103 24 0.4230891091975195 1103 25 0.4695395817969725 1104 1 0.23490904906205046 1104 2 0.23348755868393667 1104 3 0.509717483948819 1104 4 0.3583715043631557 1104 5 0.2605298590653504 1104 6 0.15393124148708637 1104 7 0.12785512048989034 1104 8 0.2614228402774472 1104 9 0.5526871445756427 1104 10 0.5502287030157831 1104 11 0.2740748521394778 1104 12 0.46097675115058934 1104 13 0.3018682607240777 1104 14 0.5922229150534974 1104 15 0.5734721051865392 1104 16 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0.33052214757886844 2207 7 0.2884467919584921 2207 8 0.37775663400985593 2207 9 -1.2121467965137995 2207 10 -0.17237333622956896 2207 11 0.21614686060847532 2207 12 0.4844783647467352 2207 13 0.5909939350107933 2207 14 0.8628444684751884 2207 15 0.787989485251849 2207 16 0.7451485520299173 2207 17 0.8191726471004799 2207 18 0.9026369291867269 2207 19 -0.37543822285910977 2207 20 0.2357240113578385 2207 21 -0.3416173880246589 2207 22 0.2595810888286607 2207 23 -0.5754035943445288 2207 24 -1.6322818725806414 2207 25 -0.32045034613501855 2208 1 0.42240140060072306 2208 2 0.23540339980143443 2208 3 0.6923766743278392 2208 4 0.5839441969147572 2208 5 0.4402135708051702 2208 6 0.3225288341573602 2208 7 0.33306730607300494 2208 8 0.5668177831935708 2208 9 -0.13931401278043865 2208 10 0.09377819367975349 2208 11 0.22805016793460453 2208 12 0.3143737705709597 2208 13 0.544067345113902 2208 14 0.8415818376848776 2208 15 0.7484304309168479 2208 16 0.5166560135403273 2208 17 0.4483679259057851 2208 18 0.7513267848531342 2208 19 -1.3808129032782972 2208 20 -0.06584005543041371 2208 21 -0.10590505465982858 2208 22 0.2520160561012972 2208 23 0.3948308685675954 2208 24 0.537955867047998 2208 25 0.47401126453498676 2209 1 0.20770898874029697 2209 2 -1.3472217050978266 2209 3 -0.20446244764747945 2209 4 0.1525147304643184 2209 5 0.18172358560282476 2209 6 0.19465977309158689 2209 7 0.19973464096121774 2209 8 0.3956398433989463 2209 9 -1.985852527227152 2209 10 -0.3325358927991715 2209 11 0.022361465395036362 2209 12 0.3535139837290231 2209 13 0.45074000964502403 2209 14 0.6543535907157221 2209 15 0.8147485105955852 2209 16 0.6849024481175192 2209 17 0.5105089584417257 2209 18 0.5891648243095418 2209 19 0.49727145761848845 2209 20 0.3696695372355592 2209 21 0.45746449743356976 2209 22 0.36860409728586774 2209 23 0.326411721762085 2209 24 0.501420191390757 2209 25 0.5301645999513269 2210 1 0.05232244922420293 2210 2 0.08876327907001169 2210 3 0.02451082622967249 2210 4 0.1575655035629424 2210 5 0.13525318841460177 2210 6 0.0016802050743720542 2210 7 0.07930492863702909 2210 8 0.15563721623050952 2210 9 0.25803394694107595 2210 10 -1.5735808298842784 2210 11 -0.5433837128663228 2210 12 -2.4252934454584434 2210 13 -0.8193401953467259 2210 14 -0.5567895854116287 2210 15 -0.7721067641754615 2210 16 -0.18409940766954236 2210 17 -9.47374549774338E-4 2210 18 0.26416494244280386 2210 19 0.13255455446632425 2210 20 0.18497037597017396 2210 21 0.08999913756278488 2210 22 0.10052012828034407 2210 23 0.0534015238076222 2210 24 0.13353491100232873 2210 25 0.06783310408000176 2211 1 -0.22171213554117486 2211 2 0.01946840781633341 2211 3 -1.3242275126596574 2211 4 -0.5759631589732592 2211 5 -0.49339127090772195 2211 6 -0.38282812887579354 2211 7 -0.35350734474589984 2211 8 -0.04933911731108509 2211 9 0.21940894024097446 2211 10 0.10744446538140551 2211 11 -0.2152176029773969 2211 12 -0.2560218918574222 2211 13 -0.22924078699345785 2211 14 -0.6952252160414645 2211 15 -0.2862938242480837 2211 16 -0.040387438841833045 2211 17 -0.13167369594471268 2211 18 -0.040406403389610546 2211 19 -0.047638915748180466 2211 20 -0.020380363044154137 2211 21 -0.040536794344902205 2211 22 -0.11928879383378188 2211 23 -0.4077395453630075 2211 24 -0.21833139757824682 2211 25 -1.4332833622177077E-5 2212 1 -0.47844213665550583 2212 2 -0.46243117122545385 2212 3 -2.5900058809016855 2212 4 -1.5366582963620274 2212 5 -0.7035482786765144 2212 6 -0.4619386464897062 2212 7 -0.380541878332636 2212 8 -0.3887675884936783 2212 9 -1.041254265141582 2212 10 -0.2747921681778891 2212 11 -0.3977500549085704 2212 12 -0.096498876304274 2212 13 -0.4712814161230554 2212 14 -0.1818277795732186 2212 15 -0.07571421880518159 2212 16 -1.2269395785983264 2212 17 -1.3943596278945023 2212 18 -0.30999592356648975 2212 19 -0.18809147474853205 2212 20 -0.22987188350281684 2212 21 -0.06057163576716107 2212 22 -0.3641744923145893 2212 23 -0.13582756884672165 2212 24 -0.19819256284098685 2212 25 -0.1182102252474806 2213 1 -3.1126573101581627 2213 2 -1.378277312463723 2213 3 -2.298458096729224 2213 4 -0.9848977660876983 2213 5 -0.782826764973386 2213 6 -0.5403827870158651 2213 7 -0.3345618662122547 2213 8 -0.10533941358963261 2213 9 -0.2238717000784689 2213 10 -0.05905070936372425 2213 11 -0.4448100740853391 2213 12 -0.08147619190547317 2213 13 -0.5697708458570432 2213 14 -0.10817928179530156 2213 15 -0.30993016196723033 2213 16 -1.5837825721920495 2213 17 -0.9978724933198251 2213 18 -0.2405938990649048 2213 19 -0.058587972404701816 2213 20 -0.16054498825372576 2213 21 0.10835317366998254 2213 22 0.00875259846297638 2213 23 0.13037942457592067 2213 24 0.09606092395659971 2213 25 0.09655446582304705 2214 1 -0.8341763594182687 2214 2 -0.7401184583971059 2214 3 -6.2746703545065365 2214 4 -2.1807011604080415 2214 5 -1.3408398851770826 2214 6 -0.8076977611663523 2214 7 -0.5689659320957645 2214 8 -0.48970688926364403 2214 9 -0.39485834179561863 2214 10 -0.16426327177380529 2214 11 -0.8706986184791035 2214 12 -0.5683307806705074 2214 13 -5.07317236427933 2214 14 -3.8415297010331315 2214 15 -2.2569285936725763 2214 16 -2.968208817855171 2214 17 -3.321848021010773 2214 18 -1.216058928207822 2214 19 -0.684505102007023 2214 20 -0.4752099714660716 2214 21 -0.4173186358586989 2214 22 -0.4438777548724815 2214 23 -0.44403441440687175 2214 24 -0.3286990295586918 2214 25 -0.07354059302763716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1442.0 3 186.0 4 193.0 5 450.0 6 55.0 7 79.0 8 115.0 9 358.0 10 915.0 11 2124.0 12 4531.0 13 8144.0 14 12329.0 15 17190.0 16 21980.0 17 24529.0 18 25402.0 19 25819.0 20 26255.0 21 26197.0 22 27051.0 23 28280.0 24 30370.0 25 32864.0 26 36775.0 27 42200.0 28 49850.0 29 61253.0 30 76395.0 31 96243.0 32 127148.0 33 170896.0 34 233130.0 35 321583.0 36 460985.0 37 712398.0 38 1351129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57052716090474 21.03403073219849 13.367635386316303 23.027806720580468 2 14.747687886004845 21.18624191983948 41.02145609233806 23.044614101817608 3 18.415550906365908 27.41777523368508 29.42265400550323 24.74401985444578 4 12.061917018647266 17.811882924187486 38.22577116982346 31.900428887341793 5 13.066702309756037 38.562625014888965 34.780766640435786 13.589906034919213 6 30.47770164908592 38.58724902238295 17.752277488835162 13.182771839695965 7 25.695663809252494 33.49254445517362 23.386818897657218 17.424972837916666 8 24.115251326114876 37.72879751526263 21.05841071418442 17.097540444438085 9 24.73618900122166 17.37330745478345 21.66023772649589 36.230265817499 10 15.117180774943554 30.36249603510712 32.982038480302805 21.538284709646515 11 32.22512020460989 26.456828078119738 22.774935288669877 18.543116428600495 12 21.09573391264199 27.937707547784086 31.388106217110522 19.578452322463402 13 26.628319321803477 24.179026952155596 26.685539440199797 22.50711428584113 14 20.40466593626785 23.35728094325038 28.092179224280944 28.145873896200825 15 23.352873071616273 30.117909321999196 24.045865354874895 22.48335225150963 16 21.64499644714784 30.95304981712862 25.988009833051713 21.41394390267183 17 20.489187190672357 30.111672427545866 28.370311649834573 21.0288287319472 18 20.893368948026197 29.54148427959854 29.166182234765202 20.39896453761006 19 21.267410078809842 28.73706677217071 29.441399993932205 20.554123155087247 20 22.597926254494187 28.032018524058905 28.49206159328513 20.87799362816178 21 22.57972463594038 28.696059452157574 28.038516340044993 20.685699571857054 22 21.638362742266356 28.316783280430226 28.675393110517884 21.369460866785538 23 21.237059138807624 28.78367189082036 28.712537697545994 21.26673127282602 24 21.395203241122633 28.276051924268558 28.464635571044607 21.864109263564206 25 21.933816305604974 27.861914098486977 27.938871309044615 22.265398286863427 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4028.0 1 4028.0 2 4095.0 3 4162.0 4 4162.0 5 4162.0 6 6621.0 7 9080.0 8 9080.0 9 9080.0 10 13631.5 11 18183.0 12 18183.0 13 18183.0 14 28828.0 15 39473.0 16 39473.0 17 39473.0 18 60951.0 19 82429.0 20 82429.0 21 82429.0 22 116345.5 23 150262.0 24 150262.0 25 150262.0 26 194884.0 27 239506.0 28 239506.0 29 239506.0 30 285378.5 31 331251.0 32 331251.0 33 331251.0 34 369986.5 35 408722.0 36 408722.0 37 408722.0 38 434290.5 39 459859.0 40 459859.0 41 459859.0 42 464184.0 43 468509.0 44 468509.0 45 468509.0 46 488702.0 47 508895.0 48 508895.0 49 508895.0 50 471926.5 51 434958.0 52 434958.0 53 434958.0 54 383213.5 55 331469.0 56 331469.0 57 331469.0 58 285615.0 59 239761.0 60 239761.0 61 239761.0 62 201504.5 63 163248.0 64 163248.0 65 163248.0 66 126598.0 67 89948.0 68 89948.0 69 89948.0 70 66965.5 71 43983.0 72 43983.0 73 43983.0 74 31303.5 75 18624.0 76 18624.0 77 18624.0 78 12840.0 79 7056.0 80 7056.0 81 7056.0 82 4715.5 83 2375.0 84 2375.0 85 2375.0 86 1564.5 87 754.0 88 754.0 89 754.0 90 493.0 91 232.0 92 232.0 93 232.0 94 137.5 95 43.0 96 43.0 97 43.0 98 38.0 99 33.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09440838602824907 2 0.0493980171280969 3 0.0526024793170453 4 0.0476232380696024 5 0.04501036890015216 6 0.06401529465153076 7 0.06108197926318568 8 0.05743382230961366 9 0.062117267047307474 10 0.06184612024670415 11 0.056645031616949434 12 0.058050065038257576 13 0.057310573763884876 14 0.06007134118820965 15 0.06263491093936836 16 0.05802541532911183 17 0.061254527227205986 18 0.14065124038568905 19 0.0654203280728389 20 0.06014529031564692 21 0.05807471474740333 22 0.06349765075946986 23 0.062462362975348074 24 0.07244549517937962 25 0.06512453156308982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4056843.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.44823979793631 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34799782544577 56.365755020360496 2 11.951834864831019 16.361641177065785 3 3.048385145340646 6.259697922742304 4 1.060297556973028 2.903020057474234 5 0.49670340540471647 1.6999236900796806 6 0.27056150133112256 1.1111675113921404 7 0.1682778320339838 0.8062824979817279 8 0.11278819946097414 0.6176122978465783 9 0.08436799283917859 0.5197356544614002 >10 0.41135136893578667 5.032186632547466 >50 0.026714243662261637 1.2637380287865387 >100 0.017017016729447356 2.144414220529049 >500 0.001670001711327048 0.813828058019498 >1k 0.0017426102578026906 2.2386854641385563 >5k 1.815219018544407E-4 0.7856484102854204 >10k+ 1.0891314111266443E-4 1.0766633562891073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 16064 0.3959729277174394 No Hit CCCATGTACTCTGCGTTGATACCAC 15370 0.3788660295702841 No Hit GTATCAACGCAGAGTACTTTTTTTT 11893 0.29315899087048725 No Hit GGTATCAACGCAGAGTACTTTTTTT 7242 0.1785131936335717 No Hit GAGTACATGGGAAGCAGTGGTATCA 6993 0.17237541605627824 No Hit TATCAACGCAGAGTACTTTTTTTTT 6824 0.1682096152106453 No Hit CATGTACTCTGCGTTGATACCACTG 5496 0.13547480146508012 No Hit GCGTTGATACCACTGCTTCCCATGT 5061 0.12475217798667586 No Hit GTATCAACGCAGAGTACATGGGAAG 4643 0.11444859956374945 No Hit ATTCCATTCCATTCCATTCCATTCC 4355 0.10734948332977146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.4649709145756935E-5 0.0 2 0.0 0.0 0.0 2.4649709145756935E-5 0.0 3 0.0 0.0 0.0 9.859883658302774E-5 0.0 4 0.0 0.0 0.0 1.2324854572878468E-4 0.0 5 0.0 0.0 0.0 1.2324854572878468E-4 0.0 6 0.0 0.0 0.0 1.2324854572878468E-4 0.0 7 0.0 0.0 0.0 1.478982548745416E-4 0.0 8 0.0 0.0 0.0 2.2184738231181242E-4 0.0 9 0.0 0.0 0.0 2.711468006033263E-4 0.0 10 0.0 0.0 0.0 2.711468006033263E-4 0.0 11 0.0 0.0 0.0 2.711468006033263E-4 0.0 12 0.0 0.0 0.0 3.2044621889484013E-4 0.0 13 0.0 0.0 0.0 3.450959280405971E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCTA 375 0.0 11.151337 16 TAGACTG 520 0.0 11.141199 5 TGTCGCC 245 0.0 10.466282 10 TGTCCCG 400 0.0 10.446771 11 CGTATAC 75 0.0026548554 10.130702 3 GCGTCGG 210 6.548362E-11 9.505389 18 AGCGTCG 230 3.6379788E-12 9.503981 17 TAGGACG 110 6.8623754E-5 9.497416 4 GGTCGAT 80 0.004529627 9.497065 11 TAGTGCG 80 0.004529627 9.497065 7 CTGTCGC 265 0.0 9.317989 9 GTCTTAG 390 0.0 9.256746 1 GTCCCGC 455 0.0 9.190435 12 CTTAGAC 240 1.0913936E-11 9.101803 3 GTACTAG 345 0.0 9.087287 1 ACCGTCC 115 1.1152083E-4 9.084149 8 AGGACAG 840 0.0 9.045158 5 CCCGCCC 475 0.0 9.003549 14 GTATTAG 605 0.0 8.950739 1 TAGACAG 605 0.0 8.947978 5 >>END_MODULE