FastQCFastQC Report
Wed 25 May 2016
SRR1294616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4056843
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT266640.6572598446624629No Hit
GGTATCAACGCAGAGTACTTTTTTT181470.4473182718680511No Hit
TATCAACGCAGAGTACTTTTTTTTT170100.4192915525693255No Hit
CCCATGTACTCTGCGTTGATACCAC167850.41374536801153017No Hit
GTACATGGGAAGCAGTGGTATCAAC157970.38939145537552233No Hit
ACGCAGAGTACTTTTTTTTTTTTTT96320.2374259984919308No Hit
GAGTACATGGGAAGCAGTGGTATCA77310.19056690140584687No Hit
CATGTACTCTGCGTTGATACCACTG62140.15317329263173357No Hit
GCGTTGATACCACTGCTTCCCATGT53690.132344288403569No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49950.12312529718305588No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47170.11627267804053547No Hit
ACGCAGAGTACATGGGAAGCAGTGG46500.11462114752776975No Hit
GTATCAACGCAGAGTACATGGGAAG45730.11272311992354646No Hit
ATTCCATTCCATTCCATTCCATTCC41960.1034301795755961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCT600.00578285511.1077659
GCCCTAG1803.8744474E-1010.0382041
TATCGCG3000.09.8395247
ACGACTG900.0010908719.522125
TACCGTA800.00443223359.52211957
CGTCTAT800.0044795279.5098781
TAGACAG5800.09.3579465
GAACTGT5400.09.3457856
TCTATAC5150.09.2233123
TCGCGCG3000.09.2035779
ATCGCCG1357.0264705E-69.13023219
TTAGACA4100.09.0576274
CCCCGGT3250.09.04379219
ATCGCGC3400.08.9619958
CGCGCGC3200.08.92588310
AGGACCG1502.6206708E-68.8873135
GAAATCG1502.7780752E-68.84908316
CCCCGTA5800.08.82739615
TGGACCG1304.254464E-58.789655
CTAATAC5200.08.7692433