FastQCFastQC Report
Wed 25 May 2016
SRR1294615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617026
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC394820.8551392173230126No Hit
CCCATGTACTCTGCGTTGATACCAC378880.8206148286797604No Hit
GAGTACATGGGAAGCAGTGGTATCA179770.3893631961353477No Hit
CATGTACTCTGCGTTGATACCACTG141770.3070591328703802No Hit
GCGTTGATACCACTGCTTCCCATGT118110.25581402400592934No Hit
GTATCAACGCAGAGTACATGGGAAG106920.23157764327079813No Hit
ACGCAGAGTACATGGGAAGCAGTGG93760.20307444662429883No Hit
GTATCAACGCAGAGTACTTTTTTTT93260.20199149842344402No Hit
TATCAACGCAGAGTACATGGGAAGC84510.18303990490848437No Hit
ACTCTGCGTTGATACCACTGCTTCC77620.16811687870070474No Hit
GCTTCCCATGTACTCTGCGTTGATA77610.16809521973668765No Hit
GGTATCAACGCAGAGTACATGGGAA72980.15806711939677187No Hit
GGTATCAACGCAGAGTACTTTTTTT63800.13818419042907706No Hit
ATTCCATTCCATTCCATTCCATTCC62530.13543350199890578No Hit
GTGGTATCAACGCAGAGTACATGGG56560.12250310048069904No Hit
TATCAACGCAGAGTACTTTTTTTTT54890.11888605348984391No Hit
CAGTGGTATCAACGCAGAGTACATG50830.11009251409890262No Hit
GAATGGAATGGAATGGAATGGAATG47870.10368146074984198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC855.344978E-511.1729588
AGCGCAA700.001496014110.85396710
GTATTAG6100.010.123611
CCCGTAC4450.010.03795216
TACACCG951.6493797E-49.9976155
CCGTACA4400.09.93612717
CTAGACA4350.09.8252434
TATCGCG2900.09.8248177
CGCCCTA5050.09.78631616
TGTCCCG5250.09.76793511
TAGACAG6150.09.7293865
CCCCGTA4300.09.724936515
TATTCCG3350.09.6394925
CGCGCGA800.00450497969.503412
TGTATCG3700.09.2410395
TGGACAG6300.09.1962195
TCGCGCG3000.09.1806479
GTATAGG5400.09.1487441
GAACCGG2600.09.1382813
GTCCCGC5550.09.07531912
Produced by FastQC (version 0.11.5)