##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294615_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4617026 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.28156956447722 31.0 30.0 34.0 26.0 34.0 2 30.290305924203157 31.0 30.0 34.0 26.0 34.0 3 30.300732766070627 31.0 30.0 34.0 26.0 34.0 4 33.92267165053868 35.0 35.0 37.0 28.0 37.0 5 34.28180932920889 37.0 35.0 37.0 30.0 37.0 6 34.362771836242636 37.0 35.0 37.0 30.0 37.0 7 34.23738267880666 37.0 35.0 37.0 30.0 37.0 8 34.14506047832523 37.0 35.0 37.0 29.0 37.0 9 35.264231130602255 39.0 35.0 39.0 28.0 39.0 10 35.3664659891454 39.0 35.0 39.0 28.0 39.0 11 35.63375038390514 39.0 35.0 39.0 29.0 39.0 12 35.40468279797428 39.0 35.0 39.0 27.0 39.0 13 35.268515923453755 39.0 35.0 39.0 27.0 39.0 14 36.2041134271282 39.0 36.0 41.0 27.0 41.0 15 36.18169336711554 39.0 36.0 41.0 27.0 41.0 16 36.2800306950838 39.0 36.0 41.0 27.0 41.0 17 36.26683215559107 39.0 36.0 41.0 27.0 41.0 18 36.14591470786606 39.0 36.0 41.0 27.0 41.0 19 36.39586413418508 39.0 36.0 41.0 27.0 41.0 20 36.527495621640426 39.0 36.0 41.0 27.0 41.0 21 36.550047801333584 39.0 36.0 41.0 27.0 41.0 22 36.55375061782195 39.0 36.0 41.0 28.0 41.0 23 36.52882050046935 39.0 36.0 41.0 28.0 41.0 24 36.581842943921046 39.0 36.0 41.0 28.0 41.0 25 35.3591807800086 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0156482861442129 1101 2 1.0292947797341867 1101 3 1.3445509770256336 1101 4 1.147074891891954 1101 5 0.8602964375404767 1101 6 0.8110876207319606 1101 7 0.7996463944481391 1101 8 0.8757110641752561 1101 9 1.2331740742408215 1101 10 1.1687696187243404 1101 11 0.9958078908520136 1101 12 1.237480750010235 1101 13 1.2168341029016077 1101 14 1.3531925420837965 1101 15 1.4697025943283606 1101 16 1.3232270401689235 1101 17 1.326002616036746 1101 18 1.4053973432759577 1101 19 1.2600962465456433 1101 20 1.1182704061080742 1101 21 1.067027928568713 1101 22 1.022284641156979 1101 23 1.0069275241685105 1101 24 1.025889818024737 1101 25 1.094494929724526 1102 1 0.9664629794566792 1102 2 0.8843775174971888 1102 3 1.1403772553735188 1102 4 1.0195610818348655 1102 5 0.8335688952858789 1102 6 0.7138843173377296 1102 7 0.6854230488931492 1102 8 0.7566132912069321 1102 9 1.13389740011457 1102 10 0.9763115063482388 1102 11 0.8487579476586191 1102 12 1.070764252095998 1102 13 1.01960854738725 1102 14 1.2278042561652711 1102 15 1.3447166002462367 1102 16 1.2087653497392097 1102 17 1.2507276882755605 1102 18 1.3729426590663607 1102 19 1.0413502413252829 1102 20 0.38970514530690537 1102 21 0.6666796250251252 1102 22 0.6575386004570731 1102 23 0.8236406454351766 1102 24 0.8693824192328634 1102 25 0.8779493287526989 1103 1 0.6997061397436646 1103 2 0.6909891484041637 1103 3 1.0193560025441464 1103 4 0.8077331730339452 1103 5 0.5535835717770468 1103 6 0.5103036061200328 1103 7 0.4524259972900353 1103 8 0.5865570167978049 1103 9 0.7106060723517587 1103 10 0.6760904037401332 1103 11 0.6321741020156111 1103 12 0.7997634095738064 1103 13 0.696948368931082 1103 14 0.6131662018802118 1103 15 0.8442968125910966 1103 16 0.8563696556740723 1103 17 0.8385514275824377 1103 18 0.9721459484604154 1103 19 0.7622051424405853 1103 20 0.00932593427394579 1103 21 0.3235004292710215 1103 22 -0.1069063510071544 1103 23 0.5191606682330914 1103 24 0.6124204166929701 1103 25 0.6336314400196983 1104 1 0.31039037174866735 1104 2 0.2780244771942826 1104 3 0.5831789818605522 1104 4 0.3539045661877722 1104 5 0.2752274176864802 1104 6 0.11155167951362444 1104 7 0.19987694856934013 1104 8 0.3184889287311137 1104 9 0.5869604567746265 1104 10 0.48033017242558884 1104 11 0.2759159884366227 1104 12 0.41690741800667297 1104 13 0.32269497525855684 1104 14 0.669306477361225 1104 15 0.5940244185999859 1104 16 0.5191577149086726 1104 17 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-1.188878163997785 2207 10 -0.13846535656099235 2207 11 0.20615097203261 2207 12 0.5126300117283549 2207 13 0.6596299039818945 2207 14 0.9156053301973657 2207 15 0.9319865603243471 2207 16 0.8218891820519119 2207 17 0.8195495943678495 2207 18 0.8811222473063296 2207 19 -0.33892711191172964 2207 20 0.26331583524027025 2207 21 -0.23293644102604816 2207 22 0.3027645009863491 2207 23 -0.4270256641752468 2207 24 -1.4963003802072237 2207 25 -0.16906545862203615 2208 1 0.4292167084434091 2208 2 0.1708634062466885 2208 3 0.6490570567588918 2208 4 0.48137802087347126 2208 5 0.3924167032237307 2208 6 0.2544010962067489 2208 7 0.34719100138575243 2208 8 0.4647432432175336 2208 9 -0.22384141790563206 2208 10 0.05763497522050898 2208 11 0.23445151709437795 2208 12 0.19581666683043863 2208 13 0.4873300011221602 2208 14 0.7214549080298411 2208 15 0.6591542736238338 2208 16 0.4438106826685839 2208 17 0.4548078383859533 2208 18 0.6851462580845649 2208 19 -1.4564324782387033 2208 20 -0.13717538479534142 2208 21 -0.3114403889623816 2208 22 0.14615643697152336 2208 23 0.3623956291466257 2208 24 0.4033958093574199 2208 25 0.4534761417377453 2209 1 0.1516277303893645 2209 2 -1.3484548773089742 2209 3 -0.16090620303588565 2209 4 0.1993747505664345 2209 5 0.19119023437632876 2209 6 0.18590013609087208 2209 7 0.20926067275114235 2209 8 0.3877783430328279 2209 9 -1.938893279940146 2209 10 -0.2521624706430998 2209 11 0.04200184671438478 2209 12 0.3622953838396654 2209 13 0.5308545174250909 2209 14 0.6681610898581312 2209 15 0.8104237405244774 2209 16 0.7531386199170953 2209 17 0.5425197186846447 2209 18 0.7669689471087864 2209 19 0.5421042176440594 2209 20 0.44520360461253006 2209 21 0.45796012150582754 2209 22 0.3584967405424351 2209 23 0.44832121413929116 2209 24 0.49400495360214336 2209 25 0.4633258258752164 2210 1 0.12766781194839893 2210 2 0.1520404490211149 2210 3 0.061170628425305296 2210 4 0.16246364837323313 2210 5 0.12060322831106163 2210 6 0.09487136257793338 2210 7 0.07568229717436026 2210 8 0.1734639640157809 2210 9 0.24452796482174932 2210 10 -1.5233920805295824 2210 11 -0.553031434670018 2210 12 -2.374156415343151 2210 13 -0.810088116950233 2210 14 -0.4522687740241267 2210 15 -0.7299354452134423 2210 16 -0.12603698536545238 2210 17 0.09202592648324526 2210 18 0.33595082484843175 2210 19 0.19770703694401703 2210 20 0.18762922929762738 2210 21 0.2125056243141472 2210 22 0.20462046116067256 2210 23 0.10737187256925296 2210 24 0.07018900566425401 2210 25 0.001182795993670993 2211 1 -0.37267278806678306 2211 2 -0.04366999908346969 2211 3 -1.4514137339555866 2211 4 -0.6467284982320223 2211 5 -0.5487396816524992 2211 6 -0.4601207297421155 2211 7 -0.41536489875064575 2211 8 -0.10767329179425644 2211 9 0.0037492209132139465 2211 10 0.07671607214445686 2211 11 -0.3244412700066803 2211 12 -0.33931479900181927 2211 13 -0.32679432351715576 2211 14 -0.8690070425002432 2211 15 -0.42416890755427517 2211 16 -0.20410729610355816 2211 17 -0.2659382012232925 2211 18 -0.03371498139610907 2211 19 -0.11731096227256899 2211 20 -0.10887462291513827 2211 21 -0.2687051473685571 2211 22 -0.26383724768778194 2211 23 -0.6194480087050493 2211 24 -0.37862099140925665 2211 25 -0.22491682971783433 2212 1 -0.4557705627191879 2212 2 -0.408913327271037 2212 3 -2.3919547005922475 2212 4 -1.4156304064191545 2212 5 -0.630995085516588 2212 6 -0.4384135002866927 2212 7 -0.2565834375655882 2212 8 -0.3823723938972705 2212 9 -0.9412759138221887 2212 10 -0.33801376585867615 2212 11 -0.5059823462162711 2212 12 -0.15195503039026192 2212 13 -0.43409534809339334 2212 14 -0.15638770856495654 2212 15 0.06826189159299645 2212 16 -1.1916348222565816 2212 17 -1.4147064535535634 2212 18 -0.3804104329174862 2212 19 -0.31289921864363635 2212 20 -0.261122740410606 2212 21 -0.19666614772778956 2212 22 -0.4924617421844033 2212 23 -0.1572632678219108 2212 24 -0.21924161802770925 2212 25 -0.1107939656386634 2213 1 -3.213597111753767 2213 2 -1.4565389331669074 2213 3 -2.4748916469018347 2213 4 -1.13770622973653 2213 5 -0.8203832986643391 2213 6 -0.6837783342244279 2213 7 -0.43609077534595286 2213 8 -0.27777207368401946 2213 9 -0.4382045003609676 2213 10 -0.2923104201506703 2213 11 -0.5649353046972863 2213 12 -0.3062440656888299 2213 13 -0.7377301252313728 2213 14 -0.35314201736437667 2213 15 -0.4206077773881418 2213 16 -1.6529793957066445 2213 17 -1.1133826184216815 2213 18 -0.3704881218644829 2213 19 -0.26359535971506176 2213 20 -0.3372882120160554 2213 21 -0.11210671683103612 2213 22 -0.15779171734131126 2213 23 -0.032281197242440385 2213 24 -0.048254432484959864 2213 25 -0.07250822730593143 2214 1 -1.055144331689256 2214 2 -0.8840219504058595 2214 3 -6.836666804657103 2214 4 -2.3999568870640005 2214 5 -1.407772876628954 2214 6 -0.7385024118873957 2214 7 -0.599250660843822 2214 8 -0.5230947888239328 2214 9 -0.2008809045718536 2214 10 -0.16553246546951783 2214 11 -0.9225743821972969 2214 12 -0.4844381761145229 2214 13 -5.062967487017449 2214 14 -4.0417027124298315 2214 15 -2.346806237404955 2214 16 -2.8949476289252516 2214 17 -3.531440782507559 2214 18 -1.2503589355936526 2214 19 -0.8295174506405445 2214 20 -0.49141894895669935 2214 21 -0.44573735056366814 2214 22 -0.389664855079765 2214 23 -0.3193783229604392 2214 24 -0.2466219390127833 2214 25 -0.10504191845658539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1762.0 3 220.0 4 234.0 5 550.0 6 74.0 7 97.0 8 198.0 9 410.0 10 1070.0 11 2448.0 12 5061.0 13 8887.0 14 14299.0 15 19937.0 16 24545.0 17 27618.0 18 29280.0 19 29877.0 20 30412.0 21 31613.0 22 32188.0 23 33880.0 24 35955.0 25 39885.0 26 44107.0 27 50481.0 28 58919.0 29 71510.0 30 88961.0 31 111766.0 32 146104.0 33 194141.0 34 262684.0 35 361095.0 36 509922.0 37 778991.0 38 1567845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.825442279582184 21.187315631020514 12.984562763395871 23.00267932600143 2 14.29989606836777 21.840812105651402 40.81435938154178 23.044932444439052 3 18.530984263904717 28.009937537152535 29.48487817436763 23.974200024575122 4 11.953799062762412 17.920264376845356 38.42515914077525 31.700777419616976 5 12.53393435717146 38.977509712247056 35.12760705141207 13.360948879169419 6 30.39846166483774 38.36013072901213 18.041778405264704 13.199629200885427 7 25.054134239041975 33.5153304307137 24.052680617417497 17.377854712826828 8 23.801756848249266 37.93061897129106 21.741863979062767 16.525760201396913 9 24.701496402958778 17.242238776435972 22.10622568911412 35.95003913149113 10 14.844947286997446 30.664808243354337 33.53976541126285 20.95047905838537 11 31.51161659210367 26.99477419689009 22.586617583604074 18.906991627402167 12 20.768528080500285 28.57008178113172 31.931722995267815 18.729667143100183 13 26.516097544483507 24.3484327232866 26.66702353743307 22.468446194796822 14 19.99924144896252 24.073657473028092 28.46777025006177 27.45933082794762 15 24.092554017621925 29.83265173515484 24.055730593849773 22.01906365337346 16 20.88891258420869 31.45831111897102 26.92939863879057 20.723377658029722 17 20.445476669919824 30.521703718200712 28.94772602996242 20.08509358191704 18 20.821337118331662 29.694574233236352 29.696764893562104 19.78732375486988 19 20.710013386091642 29.432387232581608 30.082661716818272 19.774937664508478 20 22.245980589274446 28.456147065688754 28.706773034604975 20.59109931043182 21 21.78234322813644 29.54067825371957 28.501467110140865 20.175511408003125 22 20.8375380592719 29.123774127324303 28.545667658461817 21.493020154941984 23 20.177284451621727 29.78772377019992 28.912831785235227 21.122159992943125 24 20.696512560417883 29.5292715174372 28.528122764809154 21.24609315733576 25 21.534753574625523 28.253705754729864 27.45925783036432 22.75228284028029 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5167.0 1 5167.0 2 5313.0 3 5459.0 4 5459.0 5 5459.0 6 8151.5 7 10844.0 8 10844.0 9 10844.0 10 16166.5 11 21489.0 12 21489.0 13 21489.0 14 33864.5 15 46240.0 16 46240.0 17 46240.0 18 72138.0 19 98036.0 20 98036.0 21 98036.0 22 138490.5 23 178945.0 24 178945.0 25 178945.0 26 230393.0 27 281841.0 28 281841.0 29 281841.0 30 334571.5 31 387302.0 32 387302.0 33 387302.0 34 431771.5 35 476241.0 36 476241.0 37 476241.0 38 504305.0 39 532369.0 40 532369.0 41 532369.0 42 533180.0 43 533991.0 44 533991.0 45 533991.0 46 588915.0 47 643839.0 48 643839.0 49 643839.0 50 579134.5 51 514430.0 52 514430.0 53 514430.0 54 431188.0 55 347946.0 56 347946.0 57 347946.0 58 290508.0 59 233070.0 60 233070.0 61 233070.0 62 194203.5 63 155337.0 64 155337.0 65 155337.0 66 118378.5 67 81420.0 68 81420.0 69 81420.0 70 60278.5 71 39137.0 72 39137.0 73 39137.0 74 27475.0 75 15813.0 76 15813.0 77 15813.0 78 10658.0 79 5503.0 80 5503.0 81 5503.0 82 3673.5 83 1844.0 84 1844.0 85 1844.0 86 1188.5 87 533.0 88 533.0 89 533.0 90 345.0 91 157.0 92 157.0 93 157.0 94 100.5 95 44.0 96 44.0 97 44.0 98 36.5 99 29.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10119067988787589 2 0.05315109769795535 3 0.05637828333650276 4 0.05200317260504922 5 0.04838612561419407 6 0.06946029760282918 7 0.06569163786385435 8 0.06181468330479404 9 0.06703449363291435 10 0.06668795020864081 11 0.06079671199599049 12 0.06239947533325565 13 0.06159809366462308 14 0.06424048727470888 15 0.06746767291325628 16 0.0626810418654779 17 0.0659948633600937 18 0.14173626052788094 19 0.07108471990411143 20 0.06452205380693113 21 0.06179302434077694 22 0.0686589159341966 23 0.06681790399274339 24 0.07203771432086369 25 0.07052158683966692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4617026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.91249879774864 #Duplication Level Percentage of deduplicated Percentage of total 1 83.81955628958023 56.92395515743942 2 11.290636243961604 15.335506406877192 3 2.678507244387104 5.457123600426007 4 0.9383382485185715 2.548995806975962 5 0.38978658178790376 1.3235690383524785 6 0.22313062798129907 0.9092015102712523 7 0.1384149915650375 0.6580075563775698 8 0.08267528709740463 0.4491748268484812 9 0.06536898181722518 0.39954338091651215 >10 0.3251299161239791 3.967879634866385 >50 0.025352161855902333 1.202304762194059 >100 0.018527127836313283 2.4686842134346896 >500 0.002127011915980812 1.0005398455076266 >1k 0.001901419062086429 2.7404913248512575 >5k 3.5450185903293096E-4 1.7254191511700694 >10k+ 1.9336465038159874E-4 2.8896037834912254 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 39482 0.8551392173230126 No Hit CCCATGTACTCTGCGTTGATACCAC 37888 0.8206148286797604 No Hit GAGTACATGGGAAGCAGTGGTATCA 17977 0.3893631961353477 No Hit CATGTACTCTGCGTTGATACCACTG 14177 0.3070591328703802 No Hit GCGTTGATACCACTGCTTCCCATGT 11811 0.25581402400592934 No Hit GTATCAACGCAGAGTACATGGGAAG 10692 0.23157764327079813 No Hit ACGCAGAGTACATGGGAAGCAGTGG 9376 0.20307444662429883 No Hit GTATCAACGCAGAGTACTTTTTTTT 9326 0.20199149842344402 No Hit TATCAACGCAGAGTACATGGGAAGC 8451 0.18303990490848437 No Hit ACTCTGCGTTGATACCACTGCTTCC 7762 0.16811687870070474 No Hit GCTTCCCATGTACTCTGCGTTGATA 7761 0.16809521973668765 No Hit GGTATCAACGCAGAGTACATGGGAA 7298 0.15806711939677187 No Hit GGTATCAACGCAGAGTACTTTTTTT 6380 0.13818419042907706 No Hit ATTCCATTCCATTCCATTCCATTCC 6253 0.13543350199890578 No Hit GTGGTATCAACGCAGAGTACATGGG 5656 0.12250310048069904 No Hit TATCAACGCAGAGTACTTTTTTTTT 5489 0.11888605348984391 No Hit CAGTGGTATCAACGCAGAGTACATG 5083 0.11009251409890262 No Hit GAATGGAATGGAATGGAATGGAATG 4787 0.10368146074984198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.331792803419344E-5 0.0 5 0.0 0.0 0.0 4.331792803419344E-5 0.0 6 0.0 0.0 0.0 4.331792803419344E-5 0.0 7 0.0 0.0 0.0 4.331792803419344E-5 0.0 8 2.165896401709672E-5 0.0 0.0 8.663585606838687E-5 0.0 9 2.165896401709672E-5 0.0 0.0 1.7327171213677375E-4 0.0 10 2.165896401709672E-5 0.0 0.0 2.165896401709672E-4 0.0 11 2.165896401709672E-5 0.0 0.0 2.165896401709672E-4 0.0 12 2.165896401709672E-5 0.0 0.0 2.5990756820516063E-4 0.0 13 2.165896401709672E-5 0.0 0.0 3.248844602564508E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCC 85 5.344978E-5 11.172958 8 AGCGCAA 70 0.0014960141 10.853967 10 GTATTAG 610 0.0 10.12361 1 CCCGTAC 445 0.0 10.037952 16 TACACCG 95 1.6493797E-4 9.997615 5 CCGTACA 440 0.0 9.936127 17 CTAGACA 435 0.0 9.825243 4 TATCGCG 290 0.0 9.824817 7 CGCCCTA 505 0.0 9.786316 16 TGTCCCG 525 0.0 9.767935 11 TAGACAG 615 0.0 9.729386 5 CCCCGTA 430 0.0 9.7249365 15 TATTCCG 335 0.0 9.639492 5 CGCGCGA 80 0.0045049796 9.5034 12 TGTATCG 370 0.0 9.241039 5 TGGACAG 630 0.0 9.196219 5 TCGCGCG 300 0.0 9.180647 9 GTATAGG 540 0.0 9.148744 1 GAACCGG 260 0.0 9.13828 13 GTCCCGC 555 0.0 9.075319 12 >>END_MODULE